In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogr...In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogroup A,and 11%positive for Mannheimia heamolytica.Eight isolates were recovered from 26 P.multocida positive samples,and characterized by biochemical and molecular typing methods.Among these isolates,two strains(S13 and S14)were selected for genes(RNA16S and rpoB)sequence analysis and virulence study in mice,guinea pigs and sheep.Phylogeny study showed similarities of both S14 and S13 isolates with strains from other species.In laboratory animals,the strain S14 was more virulent than S13 and induced severe illness in sheep.The high mortality of infected mice suggests that this model may represent an alternative for testing pathogenicity and vaccine efficacy.展开更多
文摘In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogroup A,and 11%positive for Mannheimia heamolytica.Eight isolates were recovered from 26 P.multocida positive samples,and characterized by biochemical and molecular typing methods.Among these isolates,two strains(S13 and S14)were selected for genes(RNA16S and rpoB)sequence analysis and virulence study in mice,guinea pigs and sheep.Phylogeny study showed similarities of both S14 and S13 isolates with strains from other species.In laboratory animals,the strain S14 was more virulent than S13 and induced severe illness in sheep.The high mortality of infected mice suggests that this model may represent an alternative for testing pathogenicity and vaccine efficacy.
