Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powde...Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powder of fruits of Vidang （Embelia ribs）, glandular trichomes of the fruits of Kamala （Mallotus philippensis）, mature fruits of Harde （Terminalia chehula）, Saindhava and Yavakshara. The use of reversed phase C18 column eluted with gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers in 22 rain. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curves showed good linear correlation coefficients （r2〉0.995） within the tested ranges. Three markers in Vidangadi churna were quantitied with respect to Embelin （0.647%, w/w）, Rottlerin （4.419%, w/w）, and Ellagic acid （0.459%, w/w）. lntraand inter-day RSDs of retention times and peak areas were less than 3.12%. The recoveries were between 99.66% and 102.33%. In conclusion, a method has been developed for the simultaneous quantification of three markers in Vidangadi churna. The RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.展开更多
Colorectal cancer(CRC)is the third most prevalent cancer.Ongoing research aims to uncover the causes of CRC,with a growing focus on the role of gut microbiota(GM)in carcinogenesis.The GM influences CRC development,pro...Colorectal cancer(CRC)is the third most prevalent cancer.Ongoing research aims to uncover the causes of CRC,with a growing focus on the role of gut microbiota(GM)in carcinogenesis.The GM influences CRC development,progression,treatment efficacy,and therapeutic toxicities.For example,Fusobacterium nucleatum and Escherichia coli can regulate microbial gene expression through the incorporation of human small noncode RNA and potentially contribute to cancer progression.Metallic nanoparticles(MNPs)have both negative and positive impacts on GM,depending on their type.Several studies state that titanium dioxide may increase the diversity,richness,and abundance of probiotics bacteria,whereas other studies demonstrate dose‐dependent GM dysbiosis.The MNPs offer cytotoxicity through the modulation of MAPK signaling pathways,NF‐kB signaling pathways,PI3K/Akt signaling pathways,extrinsic signaling pathways,intrinsic apoptosis,and cell cycle arrest at G1,G2,or M phase.MNPs enhance drug delivery,enable targeted therapy,and may restore GM.However,there is a need to conduct well‐designed clinical trials to assess the toxicity,safety,and effectiveness of MNPs‐based CRC therapies.展开更多
Pleurotus sajorcaju(P.sajorcaju),an edible and non-toxic mushroom,was evaluated as antioxidant,antitumor,an-ti-inflammatory and antihypertensive activities.P.sajorcaju is a good source of carbohydrates,dietary fiber,e...Pleurotus sajorcaju(P.sajorcaju),an edible and non-toxic mushroom,was evaluated as antioxidant,antitumor,an-ti-inflammatory and antihypertensive activities.P.sajorcaju is a good source of carbohydrates,dietary fiber,essential amino acids,minerals,vitamin B,folic acid and steroids.Anti-inflammatory,immunomodulatory and analgesic activities of aqueous and methanolic extracts of mycelium of P.sajorcaju were investigated(data is not shown).This finding suggests that extracts of P.sajorcaju can be used against inflammatory and autoimmune disease.So,P.sajorcaju examined for its antiarthritic activity.Plant was collected and separately extracted with water and methanol.For antiarthritic activity 500 and 1 000 mg·kg-1 of both extracts were prepared and ad-ministered by oral route.Body weight,paw edema(inflammation),hematological parameter,spleen weight,radiological and histologi-cal analysis of bone damage were assessed in rats with Freund's adjuvant induced paw inflammation.Both extracts showed significant and dose-dependent anti-inflammatory and anti-arthritic effects compared to control group.展开更多
文摘Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powder of fruits of Vidang （Embelia ribs）, glandular trichomes of the fruits of Kamala （Mallotus philippensis）, mature fruits of Harde （Terminalia chehula）, Saindhava and Yavakshara. The use of reversed phase C18 column eluted with gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers in 22 rain. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curves showed good linear correlation coefficients （r2〉0.995） within the tested ranges. Three markers in Vidangadi churna were quantitied with respect to Embelin （0.647%, w/w）, Rottlerin （4.419%, w/w）, and Ellagic acid （0.459%, w/w）. lntraand inter-day RSDs of retention times and peak areas were less than 3.12%. The recoveries were between 99.66% and 102.33%. In conclusion, a method has been developed for the simultaneous quantification of three markers in Vidangadi churna. The RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.
文摘Colorectal cancer(CRC)is the third most prevalent cancer.Ongoing research aims to uncover the causes of CRC,with a growing focus on the role of gut microbiota(GM)in carcinogenesis.The GM influences CRC development,progression,treatment efficacy,and therapeutic toxicities.For example,Fusobacterium nucleatum and Escherichia coli can regulate microbial gene expression through the incorporation of human small noncode RNA and potentially contribute to cancer progression.Metallic nanoparticles(MNPs)have both negative and positive impacts on GM,depending on their type.Several studies state that titanium dioxide may increase the diversity,richness,and abundance of probiotics bacteria,whereas other studies demonstrate dose‐dependent GM dysbiosis.The MNPs offer cytotoxicity through the modulation of MAPK signaling pathways,NF‐kB signaling pathways,PI3K/Akt signaling pathways,extrinsic signaling pathways,intrinsic apoptosis,and cell cycle arrest at G1,G2,or M phase.MNPs enhance drug delivery,enable targeted therapy,and may restore GM.However,there is a need to conduct well‐designed clinical trials to assess the toxicity,safety,and effectiveness of MNPs‐based CRC therapies.
基金supported by the Department of Pharmacology, S.K. Patel College of Pharmaceutical Education and Research,Ganpat University,Gujarat,India
文摘Pleurotus sajorcaju(P.sajorcaju),an edible and non-toxic mushroom,was evaluated as antioxidant,antitumor,an-ti-inflammatory and antihypertensive activities.P.sajorcaju is a good source of carbohydrates,dietary fiber,essential amino acids,minerals,vitamin B,folic acid and steroids.Anti-inflammatory,immunomodulatory and analgesic activities of aqueous and methanolic extracts of mycelium of P.sajorcaju were investigated(data is not shown).This finding suggests that extracts of P.sajorcaju can be used against inflammatory and autoimmune disease.So,P.sajorcaju examined for its antiarthritic activity.Plant was collected and separately extracted with water and methanol.For antiarthritic activity 500 and 1 000 mg·kg-1 of both extracts were prepared and ad-ministered by oral route.Body weight,paw edema(inflammation),hematological parameter,spleen weight,radiological and histologi-cal analysis of bone damage were assessed in rats with Freund's adjuvant induced paw inflammation.Both extracts showed significant and dose-dependent anti-inflammatory and anti-arthritic effects compared to control group.
