The study of tumor nanovaccines(NVs)has gained interest because they specifically recognize and eliminate tumor cells.However,the poor recognition and internalization by dendritic cells(DCs)and insufficient immunogeni...The study of tumor nanovaccines(NVs)has gained interest because they specifically recognize and eliminate tumor cells.However,the poor recognition and internalization by dendritic cells(DCs)and insufficient immunogenicity restricted the vaccine efficacy.Herein,we extracted two molecular-weight Astragalus polysaccharides(APS,12.19 k D;APSHMw,135.67 k D)from Radix Astragali and made them self-assemble with OVA257–264directly forming OVA/APS integrated nanocomplexes through the microfluidic method.The nanocomplexes were wrapped with a sheddable calcium phosphate layer to improve stability.APS in the formed nanocomplexes served as drug carriers and immune adjuvants for potent tumor immunotherapy.The optimal APS-NVs were approximately 160 nm with uniform size distribution and could remain stable in physiological saline solution.The FITC-OVA in APS-NVs could be effectively taken up by DCs,and APS-NVs could stimulate the maturation of DCs,improving the antigen cross-presentation efficiency in vitro.The possible mechanism was that APS can induce DC activation via multiple receptors such as dectin-1 and Toll-like receptors 2 and 4.Enhanced accumulation of APS-NVs both in draining and distal lymph nodes were observed following s.c.injection.Smaller APS-NVs could easily access the lymph nodes.Furthermore,APS-NVs could markedly promote antigen delivery efficiency to DCs and activate cytotoxic T cells.In addition,APS-NVs achieve a better antitumor effect in established B16-OVA melanoma tumors compared with the OVA+Alum treatment group.The antitumor mechanism correlated with the increase in cytotoxic T cells in the tumor region.Subsequently,the poor tumor inhibitory effect of APS-NVs on the nude mouse model of melanoma also confirmed the participation of antitumor adaptive immune response induced by NVs.Therefore,this study developed a promising APS-based tumor NV that is an efficient tumor immunotherapy without systemic side effects.展开更多
本文以Web of Science核心合集为文献源,分别以1960年1月1日至2017年12月31日、 1968年1月1日至2017年12月31日、 2008年1月1日至2018年9月1日三个时间段的科学文献为分析对象,借助CiteSpace软件,讨论了地震预测预报研究的发展进程,以1...本文以Web of Science核心合集为文献源,分别以1960年1月1日至2017年12月31日、 1968年1月1日至2017年12月31日、 2008年1月1日至2018年9月1日三个时间段的科学文献为分析对象,借助CiteSpace软件,讨论了地震预测预报研究的发展进程,以10年为单位的研究议程的演变,以及汶川地震以来地震预测预报研究的主要领域,试图对地震预测预报研究以及相关领域发展战略研究提供一定的参考。结果显示,地震预测预报相关科技进程一直处在动态的发展中,在重大事件的推动下,每一个时间段中呈现不同的且趋向于多样化的发展趋势,同时愈来愈向着多种方法并重的更加深入和精细的阶段发展。展开更多
This paper deals with the application of microbiological parameters obtained by usingAODC and SEM methods, and ~3H-thymidine incorporation into DNA method for quantitative estimation ofbacterial biomass、productivity ...This paper deals with the application of microbiological parameters obtained by usingAODC and SEM methods, and ~3H-thymidine incorporation into DNA method for quantitative estimation ofbacterial biomass、productivity and growth doubbing time in order to gain better understanding of the role ofmarine bacteria and their activities in Xiamen western sea waters. The results showed that in the seawatersurface, the mean value of total coliforms was 1. 3×104 cells/ml; the mean value of bacterial productionwas 10. 5×10~8 cells/(ml·h), the growth doubling time ranged from 0. 9 to 71. 3 hours. In the bottom sea-展开更多
With the problem of robot motion control in dynamic environment represented by mobile obstacles,working pieces and external mechanisms considered, a relevant control actions design procedure has been pro-posed to prov...With the problem of robot motion control in dynamic environment represented by mobile obstacles,working pieces and external mechanisms considered, a relevant control actions design procedure has been pro-posed to provide coordination of robot motions with respect to the moving external objects so that an extension ofrobot spatial motion techniques and active robotic strategies based on approaches of nonlinear control theory canbe achieved.展开更多
A novel 3-D temperature field reconstruction method is proposed in this paper, which is based on multi-wavelength thermometry and Hopfield neural network computed tomography. A mathematical model of multi-wavelength t...A novel 3-D temperature field reconstruction method is proposed in this paper, which is based on multi-wavelength thermometry and Hopfield neural network computed tomography. A mathematical model of multi-wavelength thermometry is founded, and a neural network algorithm based on multiobjective optimization is developed. Through computer simulation and comparison with the algebraic reconstruction technique (ART) and the filter back-projection algorithm (FBP), the reconstruction result of the new method is discussed in detail. The study shows that the new method always gives the best reconstruction results. At last, temperature distribution of a section of four peaks candle flame is reconstructed with this novel method.展开更多
Topology gives the following theorem: Let X and Y be topological spaces; Let f: X→Y be a continuous map. If X is connected, then f(X) is also connected. It is called connectedness invariant theorem which is not alway...Topology gives the following theorem: Let X and Y be topological spaces; Let f: X→Y be a continuous map. If X is connected, then f(X) is also connected. It is called connectedness invariant theorem which is not always very explicit for the people in its actual applications. Lately, the writer discovered that the theorem has extensive and important applications in biology. In this paper, its applications in pharmacology, genetics and physiology will be discussed.展开更多
由南加州Rancho Santa Fe建筑风格演绎而来的兰乔圣菲别墅不像古典式豪宅那样复杂与张扬，没有任何刻意炫耀的形式，惟有质朴纯粹，充满手工韵昧与岁月痕迹的建筑语汇，仿佛在平静中诉说一段悠长久远的历史，一个意味深长的传奇，一种...由南加州Rancho Santa Fe建筑风格演绎而来的兰乔圣菲别墅不像古典式豪宅那样复杂与张扬，没有任何刻意炫耀的形式，惟有质朴纯粹，充满手工韵昧与岁月痕迹的建筑语汇，仿佛在平静中诉说一段悠长久远的历史，一个意味深长的传奇，一种阅尽辉煌的人生。展开更多
基金supported by Key Project at Central Government Level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302-2305-04)CAMS Innovation Fund for Medical Sciences(2021-1-I2M-031,2022-I2M-1-018,2022-I2M-2-002)+1 种基金Jilin Provincial Fiscal Construction Program for High-Tech Industries and Technologies(2022C041-5,20220401117YY)Hohhot Science and Technology Program(2021-Social-4)。
文摘The study of tumor nanovaccines(NVs)has gained interest because they specifically recognize and eliminate tumor cells.However,the poor recognition and internalization by dendritic cells(DCs)and insufficient immunogenicity restricted the vaccine efficacy.Herein,we extracted two molecular-weight Astragalus polysaccharides(APS,12.19 k D;APSHMw,135.67 k D)from Radix Astragali and made them self-assemble with OVA257–264directly forming OVA/APS integrated nanocomplexes through the microfluidic method.The nanocomplexes were wrapped with a sheddable calcium phosphate layer to improve stability.APS in the formed nanocomplexes served as drug carriers and immune adjuvants for potent tumor immunotherapy.The optimal APS-NVs were approximately 160 nm with uniform size distribution and could remain stable in physiological saline solution.The FITC-OVA in APS-NVs could be effectively taken up by DCs,and APS-NVs could stimulate the maturation of DCs,improving the antigen cross-presentation efficiency in vitro.The possible mechanism was that APS can induce DC activation via multiple receptors such as dectin-1 and Toll-like receptors 2 and 4.Enhanced accumulation of APS-NVs both in draining and distal lymph nodes were observed following s.c.injection.Smaller APS-NVs could easily access the lymph nodes.Furthermore,APS-NVs could markedly promote antigen delivery efficiency to DCs and activate cytotoxic T cells.In addition,APS-NVs achieve a better antitumor effect in established B16-OVA melanoma tumors compared with the OVA+Alum treatment group.The antitumor mechanism correlated with the increase in cytotoxic T cells in the tumor region.Subsequently,the poor tumor inhibitory effect of APS-NVs on the nude mouse model of melanoma also confirmed the participation of antitumor adaptive immune response induced by NVs.Therefore,this study developed a promising APS-based tumor NV that is an efficient tumor immunotherapy without systemic side effects.
文摘本文以Web of Science核心合集为文献源,分别以1960年1月1日至2017年12月31日、 1968年1月1日至2017年12月31日、 2008年1月1日至2018年9月1日三个时间段的科学文献为分析对象,借助CiteSpace软件,讨论了地震预测预报研究的发展进程,以10年为单位的研究议程的演变,以及汶川地震以来地震预测预报研究的主要领域,试图对地震预测预报研究以及相关领域发展战略研究提供一定的参考。结果显示,地震预测预报相关科技进程一直处在动态的发展中,在重大事件的推动下,每一个时间段中呈现不同的且趋向于多样化的发展趋势,同时愈来愈向着多种方法并重的更加深入和精细的阶段发展。
基金This work was supported by NSF(No.30070175),NSF of Fujian Province(No D00200001)and Ministry of Education Foundation for Univ.Key Teachers
文摘This paper deals with the application of microbiological parameters obtained by usingAODC and SEM methods, and ~3H-thymidine incorporation into DNA method for quantitative estimation ofbacterial biomass、productivity and growth doubbing time in order to gain better understanding of the role ofmarine bacteria and their activities in Xiamen western sea waters. The results showed that in the seawatersurface, the mean value of total coliforms was 1. 3×104 cells/ml; the mean value of bacterial productionwas 10. 5×10~8 cells/(ml·h), the growth doubling time ranged from 0. 9 to 71. 3 hours. In the bottom sea-
基金Sponsored by Russian Foundation of Basic Research (Grant No. 97-01-00432)
文摘With the problem of robot motion control in dynamic environment represented by mobile obstacles,working pieces and external mechanisms considered, a relevant control actions design procedure has been pro-posed to provide coordination of robot motions with respect to the moving external objects so that an extension ofrobot spatial motion techniques and active robotic strategies based on approaches of nonlinear control theory canbe achieved.
基金This project was supported by China Aeronautical Basic Science Foundation under Grant No. 00156004 by Foundation of Jiangxi Test Technology and Control Engineering Center under Grant No. KG200104002.
文摘A novel 3-D temperature field reconstruction method is proposed in this paper, which is based on multi-wavelength thermometry and Hopfield neural network computed tomography. A mathematical model of multi-wavelength thermometry is founded, and a neural network algorithm based on multiobjective optimization is developed. Through computer simulation and comparison with the algebraic reconstruction technique (ART) and the filter back-projection algorithm (FBP), the reconstruction result of the new method is discussed in detail. The study shows that the new method always gives the best reconstruction results. At last, temperature distribution of a section of four peaks candle flame is reconstructed with this novel method.
文摘Topology gives the following theorem: Let X and Y be topological spaces; Let f: X→Y be a continuous map. If X is connected, then f(X) is also connected. It is called connectedness invariant theorem which is not always very explicit for the people in its actual applications. Lately, the writer discovered that the theorem has extensive and important applications in biology. In this paper, its applications in pharmacology, genetics and physiology will be discussed.
