Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and...Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and in vivo inhibitory effect on BcaP-37 cells by BRM-SJS. Results: BRM-SJS showed an inhibitory rate of 33.8% on in vivo transplanted tumor (P<0.05, compared with control). The flow cytometry analysis of BRM-SJS treated BcaP-37 (2.5 靘ol/L, 5 mol/L, 10 靘ol/L for 48 h and 72 h) revealed typical sub-G1 peak. The specific DNA Ladders were exhibited with BRM-SJS BcaP-37 cells treated. Conclusion: BRM-SJS has marked antitumor activity on BcaP-37 and its inhibitory effects on tumor were realized by both induction of apoptosis and necrosis of the tumor cells.展开更多
文摘Objective: To investigate the antitumor activity and the mechanism of BRM-SJS on breast cancer cells. Methods: Flow cytometry, DNA agarose gel electrophoresis and other techniques were used to study the in vitro and in vivo inhibitory effect on BcaP-37 cells by BRM-SJS. Results: BRM-SJS showed an inhibitory rate of 33.8% on in vivo transplanted tumor (P<0.05, compared with control). The flow cytometry analysis of BRM-SJS treated BcaP-37 (2.5 靘ol/L, 5 mol/L, 10 靘ol/L for 48 h and 72 h) revealed typical sub-G1 peak. The specific DNA Ladders were exhibited with BRM-SJS BcaP-37 cells treated. Conclusion: BRM-SJS has marked antitumor activity on BcaP-37 and its inhibitory effects on tumor were realized by both induction of apoptosis and necrosis of the tumor cells.
