Lower Continental Crusts Involved in the Alpine Orogeny:New Insight from the Santa Lucia Nappe Metasediments(Corsica,France)

In the Alpine Corsica(France),the Santa Lucia Nappe represents a peculiar unit preserving the unique relicts of Paleozoic lower to medium continental crust.It consists of composite basement affected by Permian granulite facies metamorphic conditions unconformably covered by a Late Cretaceous clastic sequence(Tomboni Conglomerate and Tralonca Flysch)affected by polyphase deformation and low-grade-metamorphism.In this work,we present a new reconstruction of the deformation events registered by the Tralonca Flysch during the Alpine orogeny.The D1 phase was testified by rare isoclinal folds.The D2 phase produced a continuous foliation and a map-scale sheath-fold with a top-to-W sense of shear.The D3 phase produced E-verging non-cylindrical folds and S3 crenulation cleavage that is not associated to metamorphic blastesis.We present the first temperature-pressure-deformation(P-T-d)path for the Tralonca Flysch,demonstrating that the Santa Lucia Nappe underwent accretion and then first stage exhumation in the Alpine wedge during the D1 phase with pressure and temperature peaks both occurred under blueschist metamorphic conditions.The D2 phase occurred at lower pressure-temperature conditions during a second stage exhumation.This pressure-temperaturedeformation path is comparable with those of the Lower Units(i.e.,the subducted continental units of Alpine Corsica)suggesting a common geodynamic history.

Taq1B CETP Polymorphism and Cardiovascular Risk in an Endogamous Pop-ulation of Diabetic Men: A Study in Santa Rosa Del Conlara, San Luis, Argentina

Objectives: Type 2 diabetes mellitus (T2DM) patients are at increased risk of cardiovascular diseases (CVDs). Several polymorphisms in the cholesterol ester transfer protein (CETP) gene have been reported. The aim of this study was to determine the distribution and effect of the Taq1B polymorphism in the CETP gene on clinical and biochemical indicators of CVD risk in a population of endogamous-T2DM men. Methods: 102 men (57.5 ± 9.3 years old) inhabitants of Santa Rosa del Conlara, San Luis, Argentina, were recruited and assigned into two groups (22 control and 80 T2DM). Further, these two groups were subdivided according to their Taq1B CETP gene genotypes (i.e., B1B1, B1B2 and B2B2). Clinical and fasting-plasma biochemical indicators of CVD risk were measured and their association with the B1 allele was determined. Results: Compared to control, T2DM men had more central obesity, hypertension, atherogenic index, insulin resistance and poorly controlled diabetes. Compared to T2DM men having the B2 allele, those T2DM men having the B1 allele have increased risk of CVD as assessed by systolic blood pressure (156 ± 16.0 vs 135.8 ± 19.2, p = 0.015), atherogenic index (6.15 ± 1.3 vs 4.4 ± 0.7, p = 0.0008), HDL-c levels (38.9 ± 5.3 vs 64.4 ± 8.2, p ± 3.0 vs 2.4 ± 0.78, p = 0.004). Interestingly, only body mass index (r = ﹣0.559, p = 0.01) and HDL-c concentration (r = ﹣0.492, p = 0.02) negatively correlated with CVD risk in the endogamous population of B1B1 and B1B2 T2DM men. Conclusion: The B1 allele of the CETP gene predicts cardiovascular complications in an endogamous population of T2DM men.

The Prevalence of Heterozygous Familial Hypercholesterolemia among Adult Filipino Patients with Dyslipidemia at Universidad de Santa Isabel Health Services Department: An Observational Descriptive Prospective Study

Objectives: It is to determine the prevalence of familial hypercholesterolemia (FH) among adult Filipino patients with dyslipidemia at Universidad de Santa Isabel Health Services Department in one year. Methods: An observational descriptive prospective study involves Filipino patients, aged 19 years and older, with dyslipidemia. The Dutch Lipid Network (DLN) Criteria was used to diagnose FH. Prevalence data and categorical variables were expressed as percentages, while continuous variables were reported as mean and standard deviations. Results: 529 patients were included in the study. 302 were females, and 227 were males. 180 (34%) scored Unlikely, 100 (19%) scored Probable, 185 (35%) scored Possible, and 64 (12%) were classified under Definite Familial Hypercholesterolemia. Most of the patients diagnosed with definite FH did not have diabetes, cerebrovascular disease (CVD), and coronary artery disease (CAD). The diagnosis was not affected by gender, BMI, smoking, and alcohol consumption. Hypertension was significantly correlated to the diagnosis of FH, as most of them were already hypertensive at diagnosis. It was noted that hypertension, diabetes, CVD, and CAD were seen at an earlier age among patients with definite FH. Conclusion: The prevalence of heterozygous FH at 12% among dyslipidemia patients and 1.3% among the general population was described for the first time in our region. This result should raise the awareness of our healthcare providers that FH, which is a major risk factor for premature CAD and CVD, exists, and early detection and management are important.

Environmental Accounting in the Context of Environmental Information： Case Study of a Reforestation Company in Campo Belo do Sul City, Santa Catarina State, Brazil

In Brazil, environmental accounting is inserted in a set of emerging matters, which are arousing great interest among researchers. One of the important points regarding the environmental issue is how to develop tools in order to analyze and manage the natural resources in a balanced way. However, in the literature there are no studies that made use of a knowledge-based approach to implement environmental accounting in organizations. This work aims to examine how environmental accounting can contribute to the knowledge creation in organizations. It held an exploratory, descriptive study and a research-action. The methodological procedures held were： literature review and development of a research-action in a forest enterprise between October 2008 and January 2011. During the interviews, the creation and change of routines and activities was pointed out, such as classification of expenditures schedules, creation of new accounts and new knowledge which help the planning and decision-making process in the organization. This work has an original character due to the practical application within an organization, in which there is a longitudinal follow up for a period of two years and three months, showing the results obtained through interviews with people involved in this process of implementing environmental accounting.

Cytochemical, Structural and Ultrastructural Characterization of Tetrasporogenesis in Bostrychia radicans (Ceramiales, Rhodophyta) from the Mangroves of Itacorubi and Rio Ratones, Santa Catarina, Brazil

Little is known about the morphology and location of macromolecules, especially proteins and carbohydrates, in vegetative and reproductive structures of mangrove species, including Bostrychia radicans. Therefore, to gain a better understanding of tetrasporogenesis in B. radicans, cytochemical, structural and ultrastructual analyses were performed. Thalli were collected from mangroves in Florianópolis, Santa Catarina, Brazil. Fertile branches were fixed and processed for light microscopy (LM), confocal microcopy and transmission electron microscopy (TEM) observations. The LM sections were stained with toluidine blue, periodic acid-Schiff and Coomassie brilliant blue. Tetrasporogenesis occurs in the pericentral cells of the terminal branches. This process is initially characterized by an increase in cell volume, resulting from the proliferation of organelles. The young tetrasporangia remain connected to the basal cell by pit connections. After a considerable increase in volume, the tetrasporocytes divide tetrahedrally, giving rise to haploid spores. During this process, there is an increasing production of starch grains, causing the organelles to group. As the organelles proliferate, the plasma membrane undergoes simultaneous invaginations toward the tetrasporangium center. The most conspicuous organelle throughout tetrasporogenesis was the Golgi complex. Polysaccharidic components are predominant in the tetrasporangium cell wall throughout tetrasporogenesis. Although protein components prevail in the cytoplasm of younger tetrasporangia, there is a predominance of reserve material with maturation. In the initial phase, there is an increase in the number of chloroplasts and a significant increment of Golgi bodies which contribute to the formation of the amorphous portion of the cell wall and possibly the biosynthesis of starch grains.

Diabetes Mellitus, Leprosy: Intensive Education Protocol in Foot at Risk, Santa Marcelina Hospital, Amazon, Brazil

Objective: To apply an intensive and multidisciplinary education protocol in order to decrease, improve, delay or cancel the beginning of neuropathy and the manifestation of lesions in diabetic patients. Methodology: This is a cross-sectional descriptive study carried out at the Diabetes Mellitus Outpatient Clinic and Ward of Santa Marcelina Hospital in Porto Velho. This research was based on cases of patients with Diabetes and Diabetics with Leprosy. The criteria used to include the patients were: being treated with insulin therapy, not to be amputated, being on high medication of the leprosy at the moment of the evaluation and to present nutritional risk classification by the screening. The population was divided into two groups of fifteen patients: eight diabetic patients, four male and four female. Seven diabetics associated with leprosy were 4 males and 3 females. The monofilament test (Semmes-Weinstein 10 g) was used as the classification basis. Results and Discussion: Group A, called the intensive care group, began diabetes education work with medical, nutritional and rehabilitation guidelines by a multidisciplinary team for an average period of 15 days in the ward and after discharged with biweekly monitoring. Group B, called conventional care, received the same guidelines in outpatient care and the monitoring followed the quarterly protocol. Both groups were evaluated and reassessed for a period of 180 days in the outpatient clinic. Group A consisted of 8 (100%) patients, 4 (50%) diabetics and 4 (50%) diabetics and leprosy patients. Conclusion: Intensive education in diabetes showed an improvement in the sensitivity, healing and nutritional status of the patients, leading to an improvement in quality of life and disability level, reducing or delaying the beginning of neurological complications. The protocol intensive method demonstrated a 100% improvement in patients in group A.

Phenology and Vegetative Growth in a New Production Region of Grapevines:Case Study in Sao Joaquim,Santa Catarina,Southern Brazil

In Brazil winemaking is expanding not only in the renewal of vineyards in traditional production areas, but also in the introduction of new crops in the newer winemaking regions. Promising characteristics were observed in the quality of the grapes and wines produced in altitude regions of Santa Catarina. The aim of this study is to characterize the geographic and climatic conditions which affect the phenology and vegetative growth of grapevine in S?o Joaquim, in the mountains region of Santa Catarina. The vineyards are located in areas with undulated and strongly undulated relief, predominantly in soils originating from basaltic rocks, in climate conditions classified as Cfb according to the K?ppen, as Region I according to Winkler classification. The phenological stage of the grapevines in the S?o Joaquim region begins later and lasts longer in comparison to other winegrowing regions in Brazil. Decrease in photoperiod and low temperatures (<10?C) were the determining factors for the induction of growth cessation of the shoots in S?o Joaquim. The geographic location in a lower latitude and its high altitude results in a distinct growing climate compared to the other winegrowing regions in Brazil.

Populational Fluctuation and Distribution of Epitrix spp.Foudras(Coleoptera:Chrysomelidae)in the Organic Tobacco Management in Santa Cruz do Sul,RS,Brazil

Among the chrysomelids, some species are considered agricultural pests, such as some representatives of Epitrix spp. Popularly known as tobacco flea, these beetles cause damage to tobacco (Nicotiana tabacum L.) by feeding on the leaf surface, causing injuries, reducing productivity and the final quality of the product to be marketed. However, studies of the biology and interactions of these individuals with the environment become essential in order to develop effective and sustainable control measures. The objective of this study was to analyze the distribution and population fluctuation of Epitrix spp., as well as to evaluate the possible influence of temperature, rainfall and adjacent vegetation on the population dynamics of this beetle in organic tobacco growing in the municipality of Santa Cruz of the South, RS. The collections were carried out weekly during the 2011/2012 harvest, using Malaise and Pit-fall traps, distributed in four points along three lines. A total of 1584 individuals belonging to the genus Epitrix were collected, and their greatest abundance was observed in late January and early February 2012 due to climatic factors, such as rainfall and temperature, favorable to the development of specimens and the tobacco leaf harvesting reduced the availability of food and conditioned a greater movement of these individuals in the crop. The results presented here are of great importance for deciding the best methodology for controlling the smoke flea, as well as knowing the influence of abiotic factors on the behavior of these organisms in the field.

Implementation of Quick HIV Tests in a Basic Health Unit in the Town of Santa Cruz-RN:Experiment Experienced by the PRO/PET-Saude Students

This article is about a report of the experiment of PRO/PET-Saúde students in the anti-HIV tests implementation in the town of Santa Cruz-RN. The goal is to report the importance and discuss the aspects of the moment experienced above inside the basic health unit in the town of Santa Cruz-RN. The experience occurred in the months from February to March 2014 and they were conducted within 8 hours per week. This way, it is observed that the period lived made possible great reflection about the making of the examination in the pre-natal appointment routines, cataloguing challenges which come up during their adequacy within the services. Lastly, the action developed aimed for the aggregation of an active learning process in the search for a critical sense in the experienced practice, allowing and prioritizing the basic health unit as a scope of reference and counter-reference in the public health service, increasing concrete answers and the needs of the population, guaranteeing an adequate service delivery and strengthening the SUS more and more. The insertion of students through the PRO/PET-Saúde-Rede Cegonha enables a reorientation of professional formation, from the integration between service and teaching.

Auditory Gain, Quality of Life, and Audiological Benefits in Bone-Anchored Hearing Device Users in Fundación SantaféDe Bogotá, Colombia

The objective of this study is to determine the auditory gain, quality of life, audiological benefits, in bone-anchored hearing device users (BAHA). It is a retrospective and concurrent evaluation of thirty patients fitted unilaterally and seven fitted bilaterally for at least six months. Patients were assessed with audiometric testing and application of Glasgow Benefit Inventory (GBI) and Abbreviated Profile of Hearing Aid Benefit (APHAB). Regarding sound-field pure audiometry results, we found a statistically significant gain in all frequencies using the bone-anchored device. APHAB scores showed statistically significant subjective audiological gains in all subscales except for the aversiveness subscale. GBI mean scores for all items in both groups were all above 3, suggesting quality of life improvement in conductive and mixed hearing loss patients. BP100 users showed a greater clinical gain in the APHAB global score and subscales compared with Divino users. In conclusion the BAHA provides significant auditory gain, subjective audiological benefits and improves quality of life in all BAHA users. This study shows a significant clinical and statistical benefit of BAHA measured by audiometric testing and by the APHAB and GBI questionnaires.

Maternal Death and Potential Years of Life Lost(PYLL)in Santa Catarina,Brazil,in 2000 and 2014

Introduction: Indicators of maternal health are often used to evaluate the social development and overall health of a population, as well as the accessibility of health services. Among these indicators, the Potential Years of Life Lost, which is associated with maternal deaths, is useful for the definition of priorities, monitoring, evaluation, and intervention, identifying the highest risk groups. Objective: To analyze the Potential Years of Life Lost by maternal death in Santa Catarina in 2000 and 2014. Method: An Ecological study with exploratory spatial analysis was conducted with data obtained from the Information System on Mortality and Live Births. Results: In the Information System, 35 maternal deaths in the year 2000 were identified and 24 in 2014. The total estimated years of life lost were 845 years in 2000 and 780 years in 2014, dominated by direct obstetric causes. In 2000, women who died lost, on average, 39.8 years of life;and 41.5 years in 2014. Conclusion: The spatial pattern observed in 2000 highlights areas of high risk in different regions of Santa Catarina. The greatest loss of years occurred in younger women, confirming the need to prevent and control maternal mortality and review strategies for compliance with public policies in the State.

Prevalence of Giardia duodenalis among dogs seized by the Center for Control of Zoonoses (CCZ) of the city of Lages, Santa Catarina, Brazil

The knowledge of the epidemiology of parasitic infections in stray and domestic animals, especially of its incidence and prevalence, is fundamental to adopting effective prophylactic measures. Stray dogs play an important role in environmental contamination favoring the transmission cycle of zoonotic agents. Among the parasitic infections that affect humans, Giardia duodenalis is the most common intestinal protozoa and was designated as a re-emerging infectious disease. This study aimed to determine the prevalence of G. duodenalis in dogs siezed by the Center for Control of Zoonoses (CCZ) of the city of Lages, Santa Catarina, Brazil using two diagnostic techniques. In 357 stool samples analysed, the prevalence of G. duodenalis cysts was 5.3% (19/357) and 4.8% (17/357) detected by floatation and sedimentation techniques, respecttively. No correspondence between gender and age was found among the methods used for analyzing the infected dogs in this study. Our data suggested that two diagnostic techniques should be used in a complementary way to ensure that false negatives are not neglected.

CLUE-S model based on GIS applied to management strategies of territory with oil wells—Case study:Santa Elena,Ecuador

Some cities worldwide have oil wells directly affecting the management of the territory.For example,La Libertad and Salinas districts contain 467 oil wells in urban areas representing a major land-use planning challenge.The objective is to apply the CLUE-S land use model in coastal cities with oil wells(Salinas-La Libertad),using geographic information systems considering environmental and security characteristics for territorial development.The stages of the study are:i)classification and categorisation of oil wells;ii)application of the GIS-CLUE-S method and visualisation of land use dynamics;iii)use the SWOT-TOWS matrix,for the analysis of the situation and the main factors affecting the territory.The results indicate high vulnerability in many urban sectors and those close to the coastline.Furthermore,the CLUE-S analysis shows that the population growth in the urban sector is close to oil well areas,making it a complex pole of human-industry interaction that impacts the management of the territory.This study synthesises three technical aspects:some oil wells do not comply with municipal ordinance regulations;identification of vulnerable zones due to environmental and security factors,which recommends a territorial reordering policy;as well as an education plan for the application of territorial ordering policies,with awareness and sustainability projections.

利用区域地震资料监测朝鲜地下核试验的研究进展

根据朝鲜6次地下核试验在中国东北及邻近地区产生的宽频带区域地震资料,中国科学院地质与地球物理研究所地震学学科组发展了一套用于监测地下核试验的地震学方法,主要包括震级测量和当量估计、高精度震中定位以及爆炸与天然地震事件识别等.在全球范围内,存在多个用于震级-当量转换的经验公式,确定何种公式适合于朝鲜半岛地区至关重要.通过搜集一组用于地震测深的化学爆炸的当量和埋深,并以这些已知爆炸源作为量规事件,能够标定朝鲜地下核试验场的震级-当量关系,从而实现对朝鲜地下核爆进行当量估计.在精确定位方面,发展了利用多事件之间波形互相关方法精确测定它们之间的走时差从而完成高精度相对震中定位.通过进一步利用Pn和Pg等多震相数据还实现了对核爆深度的相对定位.在爆炸震源识别方面,利用统计处理方法分析研究了不同类型事件所产生地震波的特征,包括6次地下核试验、4次天然地震和3次化学爆炸,以及一些矿区塌陷等.结果发现P和S类型波的振幅比,例如Pn/Lg、Pg/Lg和Pn/Sn等,在2 Hz以上能够较好区分各类震源.其中爆炸震源产生的振幅比显著高于天然地震的值.特别是,通过计算谱振幅比的台网平均值能够显著提高识别的可靠性.利用中国东北和附近地区地震台网所提供的数据可以准确地将朝鲜地下核试验从周边天然地震事件中识别出来.该方法同时得到化学爆炸的P/S谱振幅比值介于核爆炸和天然地震的群组之间.

How dopamine tunes parvalbumin interneurons in the hippocampus:new experimental observations in Alzheimer's disease

Despite decades of dedicated resea rch,Alzheimer's disease (AD) is an age-related and progressive neurodegenerative disorder for which the mechanisms of onset are sti unc ear.AD is cha racterized by featured histological alterations including amyloid-beta (AB) plaque deposition,accumulation of neurofibrillary to ngles of hyperphosphorylated-tau,and neuronal loss,accompanied by progressive cognitive decline and behavioral changes.

Permutation orbifolds of vertex operator superalgebras and associative algebras

Let V be a vertex operator superalgebra and g=(12···k)be a k-cycle which is viewed as an automorphism of the tensor product vertex operator superalgebra V■k.In this paper,we construct an explicit isomorphism from Ag(V■k)to A(V)if k is odd and to Aσ(V)if k is even whereσis the canonical automorphism of V of order 2 determined by the superspace structure of V.These recover previous results by Barron(2016)and Barron and Vander Werf(2014)that there is a one-to-one correspondence between irreducible g-twisted V■k-modules and irreducible V-modules(resp.irreducibleσ-twisted V-modules)when k is odd(resp.even).This explicit isomorphism is expected to be useful in our further study on the Zhu algebra of the fixed point subalgebra.

Assessment of skeletal muscle alterations and circulating myokines in metabolic dysfunction-associated steatotic liver disease:A crosssectional study

BACKGROUND Skeletal muscle alterations(SMAs)are being increasingly recognized in patients with metabolic dysfunctionassociated steatotic liver disease(MASLD)and appear to be associated with deleterious outcomes in these patients.However,their actual prevalence and pathophysiology remain to be elucidated.AIM To determine the prevalence of SMAs and to assess the significance of circulating myokines as biomarkers in patients with MASLD.METHODS Skeletal muscle strength and muscle mass were measured in a cross-sectional study in a cohort of 62 patients fulfilling MASLD criteria,recruited from the outpatient clinics of a tertiary level hospital.The degree of fibrosis and liver steatosis was studied using abdominal ultrasound and transitional elastography.Anthropometric and metabolic characteristics as well as serum levels of different myokines were also determined in the MASLD cohort.Statistical analysis was performed comparing results according to liver fibrosis and steatosis.RESULTS No significant differences were found in both skeletal muscle strength and skeletal muscle mass in patients with MASLD between different stages of liver fibrosis.Interestingly,serum levels of fibroblast growth factor-21(FGF21)were significantly higher in patients with MASLD with advanced hepatic fibrosis(F3-F4)than in those with lower fibrosis stages(F0-F2)(197.49±198.27 pg/mL vs 95.62±83.67 pg/mL;P=0.049).In addition,patients with MASLD with severe hepatosteatosis(S3)exhibited significantly higher serum levels of irisin(1116.87±1161.86 pg/mL)than those with lower grades(S1-S2)(385.21±375.98 pg/mL;P=0.001).CONCLUSION SMAs were uncommon in the patients with MASLD studied.Higher serum levels of irisin and FGF21 were detected in patients with advanced liver steatosis and fibrosis,respectively,with potential implications as biomarkers.

Personal identification and missing persons initiatives in Santa Catarina state,Brazil:forensic perspectives from 2019 to 2021

Santa Catarina is a small,developed,and relatively safe state in South Brazil.Despite having positive social economic indicators,it still faces multiple challenges regarding forensic practices for personal identification.The objective of this paper is to discuss the recent advances and current challenges in the region,from the perspectives of anthropological and dental postmortem human identification,missing persons,and disaster victim identification(DVI)from 2019 to 2021.The recent creation of a Forensic Anthropology Sector(SAF)in the state’s official forensic institution(Polícia Científica-PCI)has significantly improved identification of unidentified remains and optimised resources available for DNA analysis.However,SAF is still quite understaffed,which negatively affects the recovery of skeletal material,its preparation,and the time needed for filing reports.Santa Catarina has passed legislation for missing persons in 2015,4 years prior to the sanction of federal laws implementing the national policy for the disappeared.Nonetheless,a lack of integration between stakeholders remains a problem that PCI has tried to circumvent with the Conecta Programme,a multidisciplinary and integrated initiative between families of the missing persons,police agencies,and the Public Ministry.The programme aims to collect not only reference DNA samples,but also relevant anthropological and dental data.It also offers facial progression services in cases of disappearances that occurred many years ago.Despite a history of disasters in the state,PCI still needs to implement international DVI standards at an institutional level.Recent training on Phase 1 DVI procedures,integrated with other responding institutions,indicates better preparation for future disasters.There are many challenges ahead for Santa Catarina’s forensic institution and professionals that have yet to be addressed,but the overall situation on routine personal identification,missing persons initiatives,and DVI has improved over the last 2 years.

人口普查遗漏的组合式估计方法

以人口普查标准时点上的人口总体(全国、全国以下各行政区)为研究对象,研究如何利用对总体登记的普查人口名单和覆盖调查人口名单构造的组合式遗漏估计量估计人口普查遗漏人口数。目前各国政府统计部门在人口普查遗漏估计中主要使用单重遗漏估计量,但该方法未包括同时遗漏于这两份调查人口名单的双重遗漏人口,低估了总体普查遗漏人口数。组合式遗漏估计量除了包括单重遗漏人口外,还包括了双重遗漏人口,避免了单重遗漏估计量的缺陷。采取抽样估计和数理模型相结合的方法研究单重遗漏估计量、双重遗漏估计量和组合式遗漏估计量,理论和数据分析结果表明:组合式遗漏估计量在抽样估计精度和遗漏人口数估计上优于单重遗漏估计量,尤其是在分析双重遗漏人口特征和改进未来人口普查登记工作等方面;在使用刀切法近似计算总体的组合式遗漏估计量的抽样方差时,不能忽视等概率人口层之间的协方差,否则可能低估或高估其抽样方差;单重遗漏估计量和组合式遗漏估计量属于有偏估计量,要使用均方误差比较其估计精度。本文创新之处在于,利用原始数据全面演示了单重遗漏估计量、双重遗漏估计量和组合式遗漏估计量及其抽样方差估计量等的详细计算过程,同时讨论了其统计性质,尤其是利用设置的数据模拟了有偏性,有助于中国人口普查遗漏估计方案的科学制定及估计精度的提高。