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Analysis of dynamic smile and upper lip curvature in young Chinese 被引量:11
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作者 Ling-Zhi Liang Wen-Jie Hu +1 位作者 Yan-Ling Zhang Kwok-Hung Chung 《International Journal of Oral Science》 SCIE CAS CSCD 2013年第1期49-53,共5页
During smile evaluation and anterior esthetic construction,the anatomic and racial variations should be considered in order to achieve better matching results.The aims of this study were to validate an objective metho... During smile evaluation and anterior esthetic construction,the anatomic and racial variations should be considered in order to achieve better matching results.The aims of this study were to validate an objective method for recording spontaneous smile process and to categorize the smile and upper lip curvature of Chinese Han-nationality youth.One hundred and eighty-eight Chinese Han-nationality youths(88 males and 100 females) ranged from 20 to 35 years of age were selected.Spontaneous smiles were elicited by watching comical movies and the dynamics of the spontaneous smile were captured continuously with a digital video camera.All subjects’ smiles were categorized into three types:commissure,cuspid and gummy smile based on video editing software and final images.Subjects’ upper lip curvatures were also measured and divided into three groups:upward,straight and downward.Reliability analysis was conducted to obtain intra-rater reliabilities on twice measurements.The Pearson Chi-square test was used to compare differences for each parameters(α=0.05).In smile classification,60.6%commissure smile,33.5%cuspid smile and 5.9%gummy smile were obtained.In upper lip measurement,26.1%upward,39.9%straight and 34.0%downward upper lip curvature were determined.The commissure smile group showed statistically significant higher percentage of straight(46.5%) and upward(40.4%) in upper lip curvatures(P<0.05),while cuspid smile group(65.1%) and gummy smile group(72.7%) showed statistically significant higher frequency in downward upper lip curvature(P<0.05).It is evident that differences in upper lip curvature and smile classification exist based on race,when comparing Chinese subjects with those of Caucasian descent,and gender. 展开更多
关键词 Chinese Han nationality image processing SMILES upper lip curvature
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Characterization and Properties of Duplex a-C:H/MAO Coatings on Magnesium Alloy using Combined Microarc Oxidation and Hybrid Magnetron Sputtering 被引量:3
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作者 杨巍 DENG Zhennan +2 位作者 YANG Zhong LI Jianping 汪爱英 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2015年第4期822-826,共5页
The combined microarc oxidation (MAO) and magnetron sputtering deposition process was used to deposit duplex a-C:H/MAO and Ti-a-C:H/MAO coatings on AM80 magnesium alloy. The microstructure, mechanical properties a... The combined microarc oxidation (MAO) and magnetron sputtering deposition process was used to deposit duplex a-C:H/MAO and Ti-a-C:H/MAO coatings on AM80 magnesium alloy. The microstructure, mechanical properties and tribological behavior of the two duplex coatings were investigated. The experimental results showed that the a-C:H and Ti-a-C:H top films on Si substrates were dense and had a low G peak position and ID/IG ratio, compared with the hydrogen-free amorphous carbon films. Numerous micropores were found on the duplex a-C:H/MAO and Ti-a-C:H/MAO coatings together with low values of hardness (H) and elastic modulus (E), which also showed good binding strength with the Mg alloy substrates. Compared to MAO treated substrate used for the protection of the Mg alloy, the duplex a-C:H/MAO and Ti-a- C:H/MAO coatings still had stable and low value of friction coefficient, even though the surface of the duplex coatings was rough and porous. Furthermore, the mechanism of friction reduction of the two duplex coatings on the Mg alloy substrates was discussed. 展开更多
关键词 magnesium alloy microarc oxidation hydrogenated amorphous carbon magnetronsputtering tribological behavior
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Mutual Effect between Neuropeptides and Inflammatory Cytokines in Neurogenic SMSCs of Human Temporomandibular Joint 被引量:2
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作者 刘志明 彭友俭 +3 位作者 龙星 李健 柯金 房维 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2014年第4期602-607,共6页
In temporomandibular disorders(TMD), pain takes place when neuropeptides stimulate synovial tissue to produce several cytokines such as interleukin(IL)-1β, IL-6 and tumor necrosis factor(TNF)-α, which activate... In temporomandibular disorders(TMD), pain takes place when neuropeptides stimulate synovial tissue to produce several cytokines such as interleukin(IL)-1β, IL-6 and tumor necrosis factor(TNF)-α, which activate neurons and glia of synovial membrane at the bilaminar regions of temporomandibular joint(TMJ). It has been reported that, after neurogenic differentiation, the synovial mesenchymal stem cells(SMSCs), deriving from TMJ, possess the same cytological features as the neuronal cells. This study examined the ability of substance P(SP) and calcitonin gene-related peptide(CGRP) to stimulate SMSCs and neurogenic SMSCs secreting inflammatory cytokines during TMD, evaluated the mutual effects of inflammatory cytokines and neuropeptides and tested the analgesic effect of hyaluronic acid(HA). The levels of IL-1β, IL-6 and TNF-α in SMSCs and neurogenic SMSCs in the presence of neuropeptides were measured by ELISA. SP and CGRP produced by SMSCs and neurogenic SMSCs were determined by RT-PCR and Western blotting. The results showed that the expression of SP and CGRP was significantly enhanced in the neurogenic SMSCs in response to IL-1β, IL-6 and TNF-α, and the effect was remarkably inhibited by HA. IL-1β, IL-6 and TNF-α, in return, could be enhanced in the neurogenic SMSCs upon stimulation by SP and CGRP. Neuropeptides and inflammatory cytokines might work mutually on the TMD pain. The HA-mediated analgesic effect may be implicated in the inhibition of SP and CGRP expression in neurogenic SMSCs. 展开更多
关键词 hyaluronic acid inflammatory cytokines substance P calcitonin gene-related peptide NEUROGENESIS temporomandibular disorders
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The Behavior of New Hydrophilic Composite Bone Cements for Immediate Loading of Dental Implant 被引量:1
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作者 马毅慧 WANG Ruoli +2 位作者 程祥荣 LIU Zhilan ZHANG Yufeng 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2013年第3期627-633,共7页
We introduced the hydrophilic groups to acrylic bone cement to improve compliance and achieve more interdigitation between the bone and the acrylic bone cement in order to create better substrates for immediate loadin... We introduced the hydrophilic groups to acrylic bone cement to improve compliance and achieve more interdigitation between the bone and the acrylic bone cement in order to create better substrates for immediate loading. FTIR-ATR, contact angle, and maximum breach torque were employed for measurement. The results reveal that the introduction of hydrophilic functional groups has increased PMMA's surface hydrophilicity after contact angle test. FTIR-ATR results suggest the hydrophilic groups participate in the polymerization reactions, and maximum breach torque of the hydrophilic acrylic bone cements is near 110 Ncm torque. Those effects make it possible for conventional acrylic bone cement application in immediate loading of dental implant. 展开更多
关键词 immediate loading hydrophilic acrylic bone cement tooth implant
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Immunogenicity and Prediction of Epitopic Region of Antigen Ag Ⅰ/Ⅱ and Glucosyltransferase from Streptococcus mutans 被引量:3
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作者 曹茜茜 樊牮 +2 位作者 陈江 李宇红 樊明文 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2016年第3期416-421,共6页
The levels of Streptococcus(S.) mutans infections in saliva were evaluated and a comparison for specific antibody levels among children with different levels of S. mutans infection was made. The promising epitopic r... The levels of Streptococcus(S.) mutans infections in saliva were evaluated and a comparison for specific antibody levels among children with different levels of S. mutans infection was made. The promising epitopic regions of antigen AgⅠ/ Ⅱ(PAc) and glucosyltransferase(GTF) for potential vaccine targets related to S. mutans adherence were screened. A total of 94 children aged 3–4 years were randomly selected, including 53 caries-negative and 41 caries-positive children. The values of S. mutans and those of salivary total secretory immunoglobulin A(s Ig A), anti-PAc and anti-Glucan binding domain(anti-GLU) were compared to determine the correlation among them. It was found the level of s-Ig A against specific antigens did not increase with increasing severity of S. mutans infection, and the complete amino acid sequence of PAc and GTFB was analyzed using the DNAStar Protean system for developing specific anti-caries vaccines related to S. mutans adherence. A significantly positive correlation between the amount of S. mutans and children decayed, missing, and filled teeth index was observed. No significant difference was detected in specific s Ig A against PAc or GLU between any two groups. No significant correlation was found between such specific s Ig A and caries index. A total of 16 peptides from PAc as well as 13 peptides from GTFB were chosen for further investigation. S. mutans colonization contributed to early children caries as an important etiological factor. The level of s Ig A against specific antigens did not increase with increasing severity of S. mutans infection in children. The epitopes of PAc and GTF have been screened to develop the peptide-based or protein-based anti-caries vaccines. 展开更多
关键词 anti-caries vaccine secretory immunoglobulin A Streptococcus mutans bioinformatics
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Orthodontic camouflage versus orthognathic surgery: A comparative analysis of long-term stability and satisfaction in moderate skeletal Class III 被引量:1
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作者 Xueyan Xiong Yaxin Yu Fengshan Chen 《Open Journal of Stomatology》 2013年第1期89-93,共5页
Objective: The purpose of this study was to compare long-term stability and satisfaction between orthodontic camouflage and orthognathic surgery in treatment of moderate skeletal Class III adults. Materials and Method... Objective: The purpose of this study was to compare long-term stability and satisfaction between orthodontic camouflage and orthognathic surgery in treatment of moderate skeletal Class III adults. Materials and Methods: A total of 25 adults females who had been treated with orthodontic camouflage for Class III malocclusions were recalled at least 3 years post-treatment to evaluate stability and satisfaction with treatment outcomes. The data were compared with similar data for long-term outcomes in 21 patients with the same Class III problems who had bimaxillary surgical correction. Results: In the camouflage patients, small mean changes in skeletal landmark positions occurred over the long term, although the changes were generally much smaller than in the surgery patients. Dental changes in the surgery group were more severe than those in the camouflage group. The camouflage patients reported fewer functional or temporomandibular joint problems than did the surgery patients. Both groups reported similar levels of overall satisfaction with treatment. Conclusion: The results suggest that both camouflage and surgical treatment in moderate skeletal Class III adults can achieve satisfactory outcomes and provide long-term stability. If patients do not readily accept surgery because of potential surgical complications or financial difficulties, camouflage treatment may be an effective alternative treatment. 展开更多
关键词 MODERATE SKELETAL Class III ADULTS ORTHODONTIC CAMOUFLAGE ORTHOGNATHIC Surgery
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In vitro Characterization of PBLG-g-HA/PLLA Nanocomposite Scaffolds 被引量:1
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作者 廖岚 YANG Shuang +3 位作者 Richard J Miron WEI Junchao ZHANG Yufeng 张萌 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2014年第4期841-847,共7页
The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vit... The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vitro behaviour on bone mesenchymal stromal cells(BMSCs). The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds' potentioal for healing critical bone defects. 展开更多
关键词 hydroxyapatite poly(L-lactide) tissue engineering biomaterials
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Influence of Three Remineralization Materials on Physicochemical Structure of Demineralized Enamel
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作者 雷晋梅 GUO Jinxin +4 位作者 FU Dongjie WANG Yake DU Xijin ZHOU Liqun 黄翠 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2014年第2期410-416,共7页
This study aimed to evaluate the effect of three inorganic materials:casein phosphopeptide- amorphous calcium phosphate (CPP-ACP), calcium sodium phosphosilicate (CSP) and sodium fluoride (NaF) on their reminer... This study aimed to evaluate the effect of three inorganic materials:casein phosphopeptide- amorphous calcium phosphate (CPP-ACP), calcium sodium phosphosilicate (CSP) and sodium fluoride (NaF) on their remineralization potential. CPP-ACP, CSP and NaF were applicated in demineralized enamel for 4 h and then all enamel samples were immersed in remineralization solution. After 10 days, all samples were prepared for microhardness test, scanning electron microscopy (SEM) observation and the elemental level (%) analysis by energy dispersive X-ray spectroscopy (EDX). The surface microhardness (SMH) values of three experimental groups were significantly higher than that of control group (P〈0.05). The SMH value of NaF group was significantly higher than other experimental groups (P〈0.05). The SEM micrographs showed that three remineralization materials caused distinct morphological changes and remineralized deposits were different from each other. EDX elemental analysis showed that there were significant differences in the Ca (wt%) and Ca:P molar ratio of the enamel layer among control and experimental groups. The Ca (wt%) and Ca:P molar ratio in NaF group were significantly higher than those of other experimental groups. There were no significant differences in P (wt%) among control and experimental groups (P〈0.05). Three remineralization materials used in our study had the potential of remineralizing the demineralized enamel. NaF is the best and most feasible choice to be used in remineralizing the demineralized enamel. 展开更多
关键词 remineralization materials MICROHARDNESS surface morphology elemental analysis
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Anti-biofilm Effect of Glass Ionomer Cements Incorporated with Chlorhexidine and Bioactive Glass
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作者 黄雪清 黄翠 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2012年第2期270-275,共6页
The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated. The experiment... The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated. The experimental results showed that groups incorporated with 1% chlorhexidine exhibited a significant reduction of optical density values of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm. However, groups incorporated with 10% bioactive glass did not affect the optical density values and the biofilm formation. The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine. Nevertheless, the compressive strength was lower when the materials were incorporated with bioactive glass. It can be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early S mutans biofilm formation. Controlled release/sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass. 展开更多
关键词 glass ionomer cements BIOFILM CHLORHEXIDINE bioactive glass
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Evaluation of Ion Release from Four Dental Sealants
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作者 Liang CHEN Su-juan ZENG +1 位作者 Yu-hong LI Min-quan DU 《Current Medical Science》 SCIE CAS 2018年第3期524-529,共6页
The purpose of this study was to determine the ion release from four commercially available pit-and-fissure sealants [3M Clinpro, 3M ConciseTM, BeautiSealant (BS), and GI FX-Ⅱ)]. With each brand, 18 specimens were... The purpose of this study was to determine the ion release from four commercially available pit-and-fissure sealants [3M Clinpro, 3M ConciseTM, BeautiSealant (BS), and GI FX-Ⅱ)]. With each brand, 18 specimens were prepared. Their fluoride release in de-ionized water was measured by fluoride electrode, while the release of silicate (Si), aluminum (A1), sodium (Na), calcium (Ca), strontium (Sr), and phosphorus (P) was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) on days 1, 3, 7, 14, 21, and 28. The result showed that fluoride was not released from 3M ConciseTM. GI FX-II displayed the largest fluoride release, which, however, dropped rapidly on day 3.3M Clinpro exhibited less fluoride release than GI FX-II and BS did. At any time during the 28-day experimental period, GI FX-II released more Na than the other sealants (P〈0.001). BS ranked the second in Na release, and a small amount of Na ions was released from the 3M Clinpro and 3M ConciseTM samples. Al ions were only detected from BS and GI FX-Ⅱ, but not from the 3M Clinpro and 3M ConciseTM. Additionally GI FX-II had the largest Si release among the four brands at any time during the experimental period (P〈0.001). Ca ions were detected from 3M Clinpro and 3M ConciseTM, but not from GI FX-Ⅱ. BS released more Sr than the other sealants at any time during the experimental period (P〈0.001). All the samples released similar amounts of P continuously during these 28 days. In conclusion, based on the type and the amount of ion release, BS is the best pit-and-fissure sealant among the four brands. 展开更多
关键词 ion release dental sealant
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Diverticulum of the buccal mucosa: A case report
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作者 Yu Zhang Lin Wang Ke Liu 《World Journal of Clinical Cases》 SCIE 2020年第4期820-824,共5页
BACKGROUND A diverticulum is the medical or biological term for outpouching of a hollow structure in the body.It particularly occurs in the digestive system,but rarely occurs in the oral mucosa.CASE SUMMARY This repor... BACKGROUND A diverticulum is the medical or biological term for outpouching of a hollow structure in the body.It particularly occurs in the digestive system,but rarely occurs in the oral mucosa.CASE SUMMARY This report describes a rare case of diverticulum presenting in the buccal mucosa in a 44-year-old woman that was initially diagnosed in Stomatology Hospital,Wuhan University.We made our diagnosis under the guidance of imaging data,and the patient underwent surgical resection.CONCLUSION This report is the first confirmed case of buccal mucosal diverticulum.in addition,we elucidated that in general,idiopathic developmental abnormalities caused by succinate muscle defects are responsible for diverticulum development. 展开更多
关键词 DIVERTICULUM Buccal mucosa PATHOGENESIS Computed tomography examination Digestive system Pathological examination Case report
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Nucleus-targeted Dmp1 transgene fails to rescue dental defects in Dmp1 null mice 被引量:2
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作者 Shu-Xian Lin Qi Zhang +4 位作者 Hua Zhang Kevin Yan Leanne Ward Yong-Bo Lu Jian-Quan Feng 《International Journal of Oral Science》 SCIE CAS CSCD 2014年第3期133-141,共9页
Dentin matrix protein 1(DMP1) is essential to odontogenesis. Its mutations in human subjects lead to dental problems such as dental deformities, hypomineralization and periodontal impairment. Primarily, DMP1 is cons... Dentin matrix protein 1(DMP1) is essential to odontogenesis. Its mutations in human subjects lead to dental problems such as dental deformities, hypomineralization and periodontal impairment. Primarily, DMP1 is considered as an extracellular matrix protein that promotes hydroxyapatite formation and activates intracellular signaling pathway via interacting with avb3 integrin. Recent in vitro studies suggested that DMP1 might also act as a transcription factor. In this study, we examined whether full-length DMP1 could function as a transcription factor in the nucleus and regulate odontogenesis in vivo. We first demonstrated that a patient with the DMP1M1 V mutation, which presumably causes a loss of the secretory DMP1 but does not affect the nuclear translocation of DMP1, shows a typical rachitic tooth defect. Furthermore, we generated transgenic mice expressingNLSDMP1, in which the endoplasmic reticulum(ER) entry signal sequence of DMP1 was replaced by a nuclear localization signal(NLS) sequence, under the control of a 3.6 kb rat type I collagen promoter plus a 1.6 kb intron 1. We then crossbred theNLSDMP1 transgenic mice with Dmp1 null mice to express the NLSDMP1 in Dmp1-deficient genetic background. Although immunohistochemistry demonstrated thatNLSDMP1 was localized in the nuclei of the preodontoblasts and odontoblasts, the histological, morphological and biochemical analyses showed that it failed to rescue the dental and periodontal defects as well as the delayed tooth eruption in Dmp1 null mice. These data suggest that the full-length DMP1 plays no apparent role in the nucleus during odontogenesis. 展开更多
关键词 autosomal recessive hypophosphatemic rickets dentin matrix protein 1 development ODONTOBLAST ODONTOGENESIS
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Condylar and Occlusal Changes after High Condylectomy and Orthodontic Treatment for Condylar Hyperplasia 被引量:4
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作者 Loaye A.El.mozen 孟庆功 +2 位作者 李颖杰 龙星 陈国新 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第2期265-270,共6页
Condylar hyperplasia(CH) of human temporomandibular joint(TMJ) often occurs unilaterally, and causes occlusal disturbance and facial asymmetry. The purpose of this study was to compare the effects of high condylec... Condylar hyperplasia(CH) of human temporomandibular joint(TMJ) often occurs unilaterally, and causes occlusal disturbance and facial asymmetry. The purpose of this study was to compare the effects of high condylectomy with and without postsurgical orthodontic treatment. Forty patients were diagnosed as having active CH and treated with high condylectomy. Patients in group A(n=24) took the postsurgical orthodontic therapy immediately after surgery, and those in group B(n=16) did not take orthodontic therapy. For both groups, the mandibular ramus height on the affected side was decreased significantly after surgery. Orthodontic treatment promoted maxillary alveolar remodeling significantly by depressing alveolar bone of the affected side and increasing alveolar bone of the nonaffected side. Better improvement for facial midline deviations was observed in group A than in group B. In both groups, the condylar remodeling was observed and manifested by the smoothening of condylar surface and returning of condyle to normal position in glenoid fossa. It was concluded that high condylectomy in the treatment of active CH of TMJ improved the functional occlusion and facial aesthetic. Postsurgical orthodontic therapy could more effectively enhance maxillary alveolar and condylar remodeling, and more rapidly and meticulously establish the stable occlusal and normal position of condyle than the spontaneous remodeling. 展开更多
关键词 remodeling alveolar facial occlusion disturbance immediately mandibular photograph inferior concluded
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Residual Monomer Content and Its Release into Water from the Denture Base Nanocomposite Using Organic Montmorillonite as Reinforcement
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作者 李洪波 李志安 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2008年第6期839-843,共5页
A novel kind of denture base nanocomposite was prepared by polymethyl methyacrylate(PMMA) and cethyltrimethylammonium bromide modified organic montmorillonite (OMMT). The dispersion of montmorillonite in the polym... A novel kind of denture base nanocomposite was prepared by polymethyl methyacrylate(PMMA) and cethyltrimethylammonium bromide modified organic montmorillonite (OMMT). The dispersion of montmorillonite in the polymer matrix was characterized by x-ray diffraction (XRD) and transimission electron microscope (TEM).The content of residual MMA in nanocomposites and the amount of MMA released to water from nanocomposites were determined by gas chromatography (GC). The analysis of TEM and XRD showed that exfoliated-intercalated and intercalated nanocomposites were formed when the content of OMMT was 3% and 5% in the PMMA powder respectively. The results of GC showed that the residual MMA increased with the increase of OMMT content in the polymer matrix. After 7 days in water, the amount of MMA released into water from the nanocomposites tended to be stable. The results of one-way ANOVA and t-test showed that OMMT gave a significant increase of residual MMA concentration (p〈0.05) in nanocomposites. 展开更多
关键词 methyl methacrylate denture base resin MONTMORILLONITE gas chromatography nanocomposite
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Bone Tissue Response to the Bone-like Tissue Coating on Titanium
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作者 王晓琪 YANG Xu +3 位作者 ZHANG Shuang JIANG Tao 周毅 王贻宁 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2015年第1期203-209,共7页
The objective of this study was to investigate the effects of mineralized bone nodules, formed in vitro by bone marrow stromal cells (BMSCs), on the new bone formation in bone defect and on implant surface. The mine... The objective of this study was to investigate the effects of mineralized bone nodules, formed in vitro by bone marrow stromal cells (BMSCs), on the new bone formation in bone defect and on implant surface. The mineralized bone nodules were generated by culture of Lewis rats BMSCs on titanium disks in osteogenic induction medium. The gap-healing animal model was used to create the bone defect facing the disk. The titanium disks in the presence orB group or in the absence of NB group bone nodules were randomly placed into one of the rat distal femurs. This self-control design was used to compare the bone formation in defects and on titanium surface, by Micro-CT, fluorescence staining, histological and histomorphometric analysis. The new bone formation parameters in bone defect area of B group were significantly higher than those of NB group at 2 weeks, including bone volume fraction, trabecular thickness and bone area ratio. The bone nodules pre-stained with Alizarin red disappeared mostly at 2 weeks, while the red fluorescence reappeared in the newly formed bone away from the disk surface. For the bone-implant contact, B group showed lower values than NB group at 2 weeks, but no significant difference was found at 4 weeks. Our results indicate that the mineralized bone nodules can be resorbed in vivo and promote the early osteogenesis in the bone defects, and bone nodules may be applicable for new bone generation in bone defect or modification of tissue engineering scaffold. 展开更多
关键词 bone regeneration bone marrow stromal cells mineralized bone nodules BIOMATERIALS
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3D-printed surface promoting osteogenic differentiation and angiogenetic factor expression of BMSCs on Ti6Al4V implants and early osseointegration in vivo 被引量:7
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作者 Jinkai Zhang Wenhui Zhou +2 位作者 Hui Wang Kaili Lin Fengshan Chen 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2019年第2期336-343,共8页
Three-dimensional-printed(3 D-P) titanium implants display many advantages, such as design flexibility,higher efficiency, the capability to easily construct complex or customized structures, etc., and is believed to p... Three-dimensional-printed(3 D-P) titanium implants display many advantages, such as design flexibility,higher efficiency, the capability to easily construct complex or customized structures, etc., and is believed to potentially replace traditional implants. However, the biological performance of the 3 D-P titanium surface has not been investigated systematically. Herein, we analyzed the surface characteristics of 3 D-P Ti6 Al4 V implants and evaluated the biological responses of bone marrow derived mesenchymal stromal cells(BMSCs) to the 3 D-P surface in vitro. Moreover, after implantation into the rat femoral condyle for3 and 6 weeks, the osseointegration performance was evaluated. The results showed the 3 D-P Ti6 Al4 V implant presented distinct fluctuant macroscale rough surface and relatively better hydrophilicity which enhanced the adhesion, proliferation, osteogenic differentiation and angiogenetic factor expression of BMSCs. Moreover, the in vivo osseointegration performance was also better than that of the control group at the early stage. The present study suggested the 3 D-P titanium alloy is a promising candidate to be used as implant material. 展开更多
关键词 Three-dimensional PRINTING Titanium alloy BMSCS OSTEOGENESIS OSSEOINTEGRATION
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Stress area of the mandibular alveolar mucosa under complete denture with linear occlusion at lateral excursion 被引量:8
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作者 LU Ya-lin LOU Hang-di +2 位作者 RONG Qi-guo DONG Jian XU Jun 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第7期917-921,共5页
Background The rocking and instability of a loaded complete denture (CD) during lateral excursion reduce the bearing area under the denture base, causing localized high stress concentrations. This can lead to mucosa... Background The rocking and instability of a loaded complete denture (CD) during lateral excursion reduce the bearing area under the denture base, causing localized high stress concentrations. This can lead to mucosal tenderness, ulceration, and alveolar bone resorption, and the linear occlusion design was to decrease the lateral force exerted on the denture and to ensure denture stability. But it is not known how the bearing areas of linear occlusal CDs (LOCDs) and anatomic occlusal CDs (AOCDs) differ. The purpose of this study was to analyze and compare the distributions of the high and low vertical stress-bearing areas in the mandibular alveolar mucosa under LOCDs and AOCDs at lateral excursion.Methods Computerized tomography (CT) and finite element analysis were used to establish three-dimensional models of an edentulous maxilla and mandible with severe residual ridge resorption. These models were composed of maxillary and mandibular bone structure, mucosa, and the LOCD or AOCD. Lateral excursion movements of the mandible were simulated and the vertical stress-bearing areas in the mucosa under both mandibular CDs were analyzed using ANSYS 7.0.Results On the working side, the high stress-bearing (-0.07 to -0.1 Mpa) area under the LOCD during lateral excursion was smaller than that under the AOCD, while the medium stress-bearing (-0.03 to -0.07 Mpa) area under the LOCD was 1.33-fold that under the AOCD. The medium stress-bearing area on the non-working side under the LOCD was 2.4-fold that under the AOCD. Therefore, the overall medium vertical stress-bearing area under the LOCD was 20% larger than that under the AOCD.Conclusions During lateral excursion, the medium vertical stress-bearing area under a mandibular LOCD was larger and the high vertical stress-bearing area was smaller than that under an AOCD. Thus, the vertical stress under the LOCD was distributed more evenly and over a wider area than that under the AOCD, thereby improving denture stability. 展开更多
关键词 complete denture linear occlusion stress area distribution finite element analysis
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Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis 被引量:3
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作者 Hui Xue Dike Tao +8 位作者 Yuteng Weng Qiqi Fan Shuang Zhou Ruilin Zhang Han Zhang Rui Yue Xiaogang Wang Zuolin Wang Yao Sun 《Frontiers of Medicine》 SCIE CAS CSCD 2019年第5期575-589,共15页
Fractures are frequently occurring diseases that endanger human health. Crucial to fracture healing is cartilage formation, which provides a bone-regeneration environment. Cartilage consists of both chondrocytes and e... Fractures are frequently occurring diseases that endanger human health. Crucial to fracture healing is cartilage formation, which provides a bone-regeneration environment. Cartilage consists of both chondrocytes and extracellular matrix (ECM). The ECM of cartilage includes collagens and various types of proteoglycans (PGs), which play important roles in maintaining primary stability in fracture healing. The PG form of dentin matrix protein 1 (DMP1-PG) is involved in maintaining the health of articular cartilage and bone. Our previous data have shown that DMP1-PG is richly expressed in the cartilaginous calluses of fracture sites. However, the possible significant role of DMP1-PG in chondrogenesis and fracture healing is unknown. To further detect the potential role of DMP1-PG in fracture repair, we established a mouse fracture model by using a glycosylation site mutant DMP1 mouse (S89G-DMP1 mouse). Upon inspection, fewer cartilaginous calluses and down-regulated expression levels of chondrogenesis genes were observed in the fracture sites of S89G-DMP1 mice. Given the deficiency of DMP1-PG, the impaired IL-6/JAK/STAT signaling pathway was observed to affect the chondrogenesis of fracture healing. Overall, these results suggest that DMP1-PG is an indispensable proteoglycan in chondrogenesis during fracture healing. 展开更多
关键词 fracture extracellular MATRIX DENTIN MATRIX protein 1 PROTEOGLYCAN CARTILAGE
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Microstructure and Tribological Behavior of Self-lubricating(Si:N)-DLC/MAO Coatings on AZ80 Magnesium Substrate 被引量:3
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作者 Wei YANG Zhennan DENG +2 位作者 Dong ZHANG Peiling KE Aiying WANG 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2013年第6期693-698,共6页
The combined micro arc oxidation (MAO) and a hybrid beam deposition process was used to deposit duplex (Si:N)-DLC/MAO coatings on AZ80 magnesium alloy. The microstructure and composition of the duplex coatings we... The combined micro arc oxidation (MAO) and a hybrid beam deposition process was used to deposit duplex (Si:N)-DLC/MAO coatings on AZ80 magnesium alloy. The microstructure and composition of the duplex coatings were analyzed by Raman spectroscopy, X-ray photoelectron spectroscope (XPS), scanning electron microscope (SEM) and atomic force microscopy (AFM). Tribological behaviors of the coatings were studied by ball-on-disk friction test. It was found that the ID/IG ratio of the (Si:N)-DLC (diamond-like carbon) top films increases with decreasing C2H2/N2 ratio. The (Si:N)-DLC top film with SigN4 was formed on the MAO coated sample as the C2H2/N2 ratio was 10sccm:5sccm, which showed an increasing critical load compared with the pure DLC directly deposited on the Mg alloy substrate. As a result, the (Si:N)-DLC/MAO coating exhibited an advanced wear protection for the substrate. 展开更多
关键词 Mg alloy Micro arc oxidation DLC Coating MICROSTRUCTURE Tribologicalbehavior
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