Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology...Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology represented by the Internet of Things,cloud computing,mobile networks and big data,and urbanization.How to effectively achieve the long-term preservation of massive,heterogeneous,and multi-source digital electronic records in smart cities is a key issue thatmust be solved.Digital continuity can ensure the accessibility,integrity and availability of information.The quality management of electronic record,like the quality management of product,will run through every phase of the urban lifecycle.Based on data quality management,this paper constructs digital continuity of smart city electronic records.Furthermore,thework in this paper ensures the authenticity,integrity,availability and timeliness of electronic documents by quality management of electronic record.This paper elaborates on the overall technical architecture of electronic record,as well as the various technical means needed to protect its four characteristics.展开更多
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobac...Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.展开更多
基金the NSFC (Nos. 61772280, 62072249)the AIrecognition scoring system of weather map (No. SYCX202011)+1 种基金the national training programsof innovation and entrepreneurship for undergraduates (Nos. 201910300123Y, 202010300200)the PAPD fund from NUIST. Jinyue Xia is the corresponding author.
文摘Under the co-promotion of the wave of urbanization and the rise of data science,smart cities have become the new concept and new practice of urban development.Smart cities are the combination of information technology represented by the Internet of Things,cloud computing,mobile networks and big data,and urbanization.How to effectively achieve the long-term preservation of massive,heterogeneous,and multi-source digital electronic records in smart cities is a key issue thatmust be solved.Digital continuity can ensure the accessibility,integrity and availability of information.The quality management of electronic record,like the quality management of product,will run through every phase of the urban lifecycle.Based on data quality management,this paper constructs digital continuity of smart city electronic records.Furthermore,thework in this paper ensures the authenticity,integrity,availability and timeliness of electronic documents by quality management of electronic record.This paper elaborates on the overall technical architecture of electronic record,as well as the various technical means needed to protect its four characteristics.
基金supported by the National Natural Science Foundation of China (No. 21177009)
文摘Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.
