We successfully obtained 3D skeletal images of Hyla suweonensis, employing a nondestructive method by applying appropriate anesthesia and limiting the radiation dose. H. suweonensis is a tree frog endemic to Korea and...We successfully obtained 3D skeletal images of Hyla suweonensis, employing a nondestructive method by applying appropriate anesthesia and limiting the radiation dose. H. suweonensis is a tree frog endemic to Korea and is on the list of endangered species. Previous studies have employed caliper-based measurements and two-dimensional （2D） X-ray imaging for anatomical analyses of the skeletal system or bone types of H. suweonensis. In this work we reconstructed three-dimensional （3D） skeletal images of H. suweonensis, utilizing a nondestructive micro-computed tomography （micro-CT） with a short scan and low radiation dose （i.e. 4 min and 0.16 Gy）. Importantly, our approach can be applied to the imaging of 3D skeletal systems of other endangered frog species, allowing both versatile and high contrast images of anatomical structures without causing any significant damages to the living animal.展开更多
Sesame is an ancient oilseed crop with high oil content and quality.However,the evolutionary history and genetic mechanisms of its valuable agronomic traits remain unclear.Here,we report chromosome-scale genomes of cu...Sesame is an ancient oilseed crop with high oil content and quality.However,the evolutionary history and genetic mechanisms of its valuable agronomic traits remain unclear.Here,we report chromosome-scale genomes of cultivated sesame(Sesamum indicum L.)and six wild Sesamum species,representing all three karyotypes within this genus.Karyotyping and genome-based phylogenic analysis revealed the evolutionary route of Sesamum species from n=13 to n=16 and revealed that allotetraploidization occurred in the wild species Sesamum radiatum.Early divergence of the Sesamum genus(48.5–19.7 million years ago)during the Tertiary period and its ancient phylogenic position within eudicots were observed.Pan-genome analysis revealed 9164 core gene families in the 7Sesamumspecies.These families are significantly enriched in variousmetabolic pathways,including fatty acid(FA)metabolism and FA biosynthesis.Structural variations in SiPT1 and SiDT1 within the phosphatidyl ethanolamine-binding protein gene family lead to the genomic evolution of plant-architecture and inflorescence-development phenotypes in Sesamum.A genome-wide association study(GWAS)of an interspecific population and genome comparisons revealed a long terminal repeat insertion and a sequence deletion inDIR genes of wildSesamum angustifoliumand cultivated sesame,respectively;both variations independently cause high susceptibility toFusariumwilt disease.A GWAS of 560 sesame accessions combined with an overexpression study confirmed that the NAC1andPPOgenes play an important role in upregulating oil content of sesame.Our study provides high-quality genomic resources for cultivated and wild Sesamum species and insights that can improve molecular breeding strategies for sesame and other oilseed crops.展开更多
Retinoid X receptor a （RXRα） and its N-terminally trun- cated version tRXRα play important roles in tumorige. nesis, while some RXRg ligands possess potent anti- cancer activities by targeting and modulating the t...Retinoid X receptor a （RXRα） and its N-terminally trun- cated version tRXRα play important roles in tumorige. nesis, while some RXRg ligands possess potent anti- cancer activities by targeting and modulating the tumorigenic effects of RXRo and tRXRa. Here we describe NSC-640358 （N-6）, a thiazolyl-pyrazole derived compound, acts as a selective RXRα ligand to promote TNFα-mediated apoptosis of cancer cell. N-6 binds to RXRa and inhibits the transactivation of RXRα homod- imer and RXRa/TR3 heterodimer. Using mutational analysis and computational study, we determine that Arg316 in RXRa, essential for 9-cis-retinoic acid binding and activating RXRg transactivation, is not required for antagonist effects of N-6, whereas Trp305 and Phe313 are crucial for N-6 binding to RXRα by forming extra w-w stacking interactions with N-6, indicating a distinct RXRα binding mode of N-6. N-6 inhibits TR3-stimulated transactivation of Gal4-DBD-RXRα-LBD by binding to the ligand binding pocket of RXRa-LBD, suggesting a strategy to regulate TR3 activity indirectly by using small molecules to target its interacting partner RXRα. For its physiological activities, we show that N-6 strongly inhibits tumor necrosis factor a （TNFα）-induced AKT activation and stimulates TNFa-mediated apoptosis in cancer cells in an RXRa/tRXRo dependent manner.The inhibition of TNFα-induced tRXRα/p85α complex formation by N-6 implies that N-6 targets tRXRa to inhibit TNFα-induced AKT activation and to induce cancer cell apoptosis. Together, our data illustrate a new RXRa ligand with a unique RXRα binding mode and the abilities to regulate TR3 activity indirectly and to induce TNFa-mediated cancer cell apoptosis by targeting RXRα/tRXRα.展开更多
Neuroinflammation following immune activation in the central nervous system（CNS）leads to neuronal loss in specific areas of the CNS resulting in neurodegenerative disorders.Thus,fine tuning the immune responses with...Neuroinflammation following immune activation in the central nervous system（CNS）leads to neuronal loss in specific areas of the CNS resulting in neurodegenerative disorders.Thus,fine tuning the immune responses within the brain is essential,because most of the brain diseases are associated with chronic inflammation,展开更多
This paper reports on a new disorder that first appeared in eucalypt plantations in south China in2003. The plantations were destructively sampled near Leizhou, Yanxi, Kaiping and Gaoyao in September toDecember 2003. ...This paper reports on a new disorder that first appeared in eucalypt plantations in south China in2003. The plantations were destructively sampled near Leizhou, Yanxi, Kaiping and Gaoyao in September toDecember 2003. Whilst some 5% of 0.5 to 1.5 year-old Eucalyptus urophylla trees declined rapidly andexhibited symptoms of wilting, the majority of the affected trees declined slowly and exhibited two foliarsymptoms. Young leaves initially developed an interveinal chlorosis extending from the leaf margin inwardsto the midrib. Most leaves then developed pockets of necrotic tissue that became bleached but typically hadbrown margins. The former symptoms have been recognised as being caused by limited boron supply in soil,but the latter symptoms have not been previously observed in eucalypts in China. Inorganic analysisshowed that the leaves with the latter symptoms had deficient B concentrations whereas the leaves fromhealthy trees had adequate B concentrations. Bacterial wilt, Ralstonia solanacearum, was present at all siteswhere foliar symptoms and tree death were present. The root system of all trees with foliar chlorosis andnecrosis had signs of infection in some roots and attrition of lateral roots. The trunk and branches of therecently died trees and the trees with advanced leaf drop had discoloured xylem, which on cutting, oozedbacterial slime. Possible relationships between root damage caused by abiotic (e.g. typhoons, root damagefrom hand weeding) and biotic factors and reduced B uptake are discussed. Recommendations are made forreducing bacterial wilt disease and improving B management in fast-growing short-rotation eucalypt plantations.展开更多
基金supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (grant numbers: NRF-2015R1C1A1A01052498 and NRF2014R1A1A1006010)
文摘We successfully obtained 3D skeletal images of Hyla suweonensis, employing a nondestructive method by applying appropriate anesthesia and limiting the radiation dose. H. suweonensis is a tree frog endemic to Korea and is on the list of endangered species. Previous studies have employed caliper-based measurements and two-dimensional （2D） X-ray imaging for anatomical analyses of the skeletal system or bone types of H. suweonensis. In this work we reconstructed three-dimensional （3D） skeletal images of H. suweonensis, utilizing a nondestructive micro-computed tomography （micro-CT） with a short scan and low radiation dose （i.e. 4 min and 0.16 Gy）. Importantly, our approach can be applied to the imaging of 3D skeletal systems of other endangered frog species, allowing both versatile and high contrast images of anatomical structures without causing any significant damages to the living animal.
基金supported by earmarked funding for the China Agricultural Research System of MOF and MARA (CARS-14),Chinathe China National"973"Project (2011CB109304),China+5 种基金the Henan Zhongyuan Scientist Work Station Construction Fund (092101211100),Chinathe National Natural Science Foundation of China (U1204318,U1304321,31301653,31471537,and 32172094),Chinathe Key Project of Science and Technology of Henan Province (201300110600),Chinathe Key Research Project of the Shennong Laboratory (SN01-2022-04),Chinathe Key Research and Development Project of Henan Province (221111520400),Chinathe Innovation Scientists and Technicians Troop Construction Project of the Henan Academy of Agricultural Sciences (2023TD04),China.
文摘Sesame is an ancient oilseed crop with high oil content and quality.However,the evolutionary history and genetic mechanisms of its valuable agronomic traits remain unclear.Here,we report chromosome-scale genomes of cultivated sesame(Sesamum indicum L.)and six wild Sesamum species,representing all three karyotypes within this genus.Karyotyping and genome-based phylogenic analysis revealed the evolutionary route of Sesamum species from n=13 to n=16 and revealed that allotetraploidization occurred in the wild species Sesamum radiatum.Early divergence of the Sesamum genus(48.5–19.7 million years ago)during the Tertiary period and its ancient phylogenic position within eudicots were observed.Pan-genome analysis revealed 9164 core gene families in the 7Sesamumspecies.These families are significantly enriched in variousmetabolic pathways,including fatty acid(FA)metabolism and FA biosynthesis.Structural variations in SiPT1 and SiDT1 within the phosphatidyl ethanolamine-binding protein gene family lead to the genomic evolution of plant-architecture and inflorescence-development phenotypes in Sesamum.A genome-wide association study(GWAS)of an interspecific population and genome comparisons revealed a long terminal repeat insertion and a sequence deletion inDIR genes of wildSesamum angustifoliumand cultivated sesame,respectively;both variations independently cause high susceptibility toFusariumwilt disease.A GWAS of 560 sesame accessions combined with an overexpression study confirmed that the NAC1andPPOgenes play an important role in upregulating oil content of sesame.Our study provides high-quality genomic resources for cultivated and wild Sesamum species and insights that can improve molecular breeding strategies for sesame and other oilseed crops.
文摘Retinoid X receptor a （RXRα） and its N-terminally trun- cated version tRXRα play important roles in tumorige. nesis, while some RXRg ligands possess potent anti- cancer activities by targeting and modulating the tumorigenic effects of RXRo and tRXRa. Here we describe NSC-640358 （N-6）, a thiazolyl-pyrazole derived compound, acts as a selective RXRα ligand to promote TNFα-mediated apoptosis of cancer cell. N-6 binds to RXRa and inhibits the transactivation of RXRα homod- imer and RXRa/TR3 heterodimer. Using mutational analysis and computational study, we determine that Arg316 in RXRa, essential for 9-cis-retinoic acid binding and activating RXRg transactivation, is not required for antagonist effects of N-6, whereas Trp305 and Phe313 are crucial for N-6 binding to RXRα by forming extra w-w stacking interactions with N-6, indicating a distinct RXRα binding mode of N-6. N-6 inhibits TR3-stimulated transactivation of Gal4-DBD-RXRα-LBD by binding to the ligand binding pocket of RXRa-LBD, suggesting a strategy to regulate TR3 activity indirectly by using small molecules to target its interacting partner RXRα. For its physiological activities, we show that N-6 strongly inhibits tumor necrosis factor a （TNFα）-induced AKT activation and stimulates TNFa-mediated apoptosis in cancer cells in an RXRa/tRXRo dependent manner.The inhibition of TNFα-induced tRXRα/p85α complex formation by N-6 implies that N-6 targets tRXRa to inhibit TNFα-induced AKT activation and to induce cancer cell apoptosis. Together, our data illustrate a new RXRa ligand with a unique RXRα binding mode and the abilities to regulate TR3 activity indirectly and to induce TNFa-mediated cancer cell apoptosis by targeting RXRα/tRXRα.
基金funded by Department of Science and Technology,Government of India (Grant No.SR/CSI/59/2011(G)Gujarat State Biotechnology Mission (Grant No.GSBTM/MD/PROJECTS/.SSA/3385/2012/2013)
文摘Neuroinflammation following immune activation in the central nervous system（CNS）leads to neuronal loss in specific areas of the CNS resulting in neurodegenerative disorders.Thus,fine tuning the immune responses within the brain is essential,because most of the brain diseases are associated with chronic inflammation,
文摘This paper reports on a new disorder that first appeared in eucalypt plantations in south China in2003. The plantations were destructively sampled near Leizhou, Yanxi, Kaiping and Gaoyao in September toDecember 2003. Whilst some 5% of 0.5 to 1.5 year-old Eucalyptus urophylla trees declined rapidly andexhibited symptoms of wilting, the majority of the affected trees declined slowly and exhibited two foliarsymptoms. Young leaves initially developed an interveinal chlorosis extending from the leaf margin inwardsto the midrib. Most leaves then developed pockets of necrotic tissue that became bleached but typically hadbrown margins. The former symptoms have been recognised as being caused by limited boron supply in soil,but the latter symptoms have not been previously observed in eucalypts in China. Inorganic analysisshowed that the leaves with the latter symptoms had deficient B concentrations whereas the leaves fromhealthy trees had adequate B concentrations. Bacterial wilt, Ralstonia solanacearum, was present at all siteswhere foliar symptoms and tree death were present. The root system of all trees with foliar chlorosis andnecrosis had signs of infection in some roots and attrition of lateral roots. The trunk and branches of therecently died trees and the trees with advanced leaf drop had discoloured xylem, which on cutting, oozedbacterial slime. Possible relationships between root damage caused by abiotic (e.g. typhoons, root damagefrom hand weeding) and biotic factors and reduced B uptake are discussed. Recommendations are made forreducing bacterial wilt disease and improving B management in fast-growing short-rotation eucalypt plantations.
