[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol ...[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.展开更多
[Objectives]This study was conducted to explore the best extraction technology of total flavonoids from Morinda citrifolia L.[Methods]An orthogonal test was carried out to select the best extraction process with the y...[Objectives]This study was conducted to explore the best extraction technology of total flavonoids from Morinda citrifolia L.[Methods]An orthogonal test was carried out to select the best extraction process with the yield of total flavonoids in the extract as the investigation index.[Results]Among the four factors of experimental design,the extraction time had a very significant impact on the results,the ethanol concentration had a significant impact on the results,and the extraction temperature and ethanol dosage had no significant impacts on the results.The optimal extraction process conditions for the total flavonoids of M.citrifolia were as follows:extraction time 1.5 h,ethanol concentration 90%,extraction temperature 60-65℃,and ethanol dosage 1∶20(g/ml).The total flavonoids in M.citrifolia fruit were extracted according to the optimal extraction process conditions,and the yield was 2.936%.[Conclusions]This study provides a theoretical basis for obtaining the optimal extraction process conditions for total flavonoids of M.citrifolia.展开更多
基金College Students'Innovation and Entrepreneurship Training Program of Liaoning Province(201911430039).
文摘[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.
基金2020 National Undergraduate Innovation and Enterpreneurship Training Program (202011430133).
文摘[Objectives]This study was conducted to explore the best extraction technology of total flavonoids from Morinda citrifolia L.[Methods]An orthogonal test was carried out to select the best extraction process with the yield of total flavonoids in the extract as the investigation index.[Results]Among the four factors of experimental design,the extraction time had a very significant impact on the results,the ethanol concentration had a significant impact on the results,and the extraction temperature and ethanol dosage had no significant impacts on the results.The optimal extraction process conditions for the total flavonoids of M.citrifolia were as follows:extraction time 1.5 h,ethanol concentration 90%,extraction temperature 60-65℃,and ethanol dosage 1∶20(g/ml).The total flavonoids in M.citrifolia fruit were extracted according to the optimal extraction process conditions,and the yield was 2.936%.[Conclusions]This study provides a theoretical basis for obtaining the optimal extraction process conditions for total flavonoids of M.citrifolia.