Professor Henry N.C.Wong obtained his B.Sc.degree with First Class Honours from The Chinese University of Hong Kong(CUHK)in 1973 and his Ph.D.degree from University College London(with Professor Franz Sondheimer)in 19...Professor Henry N.C.Wong obtained his B.Sc.degree with First Class Honours from The Chinese University of Hong Kong(CUHK)in 1973 and his Ph.D.degree from University College London(with Professor Franz Sondheimer)in 1976.After working at Harvard University as a Postdoctoral Associate with Professor Robert B.Woodward,he returned to University College London to begin his independent research undertaking as Ramsay Memorial Fellow.From 1980 to 1982,Professor Wong did research at Shanghai Inst让ute of Organic Chemistry,The Chinese Academy of Sciences.In 1982,he went back to Hong Kong,and started his career at CUHK his alma mater.He retired in 2018,and is now Emeritus Professor of Chemistry and Research Profess or.展开更多
Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the un...Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post-translational regulation of the activities of transcription factors(TFs) is a dynamic and essential mechanism for plant growth and development. CRL3 BPME3 ligase is a CULLIN3-based E3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over-expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106-1 mutants, including Flowering Locus T(FT) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays(EMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction(ChIP-q PCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3 BPME3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3 BPM E3 ligases in Arabidopsis.展开更多
文摘Professor Henry N.C.Wong obtained his B.Sc.degree with First Class Honours from The Chinese University of Hong Kong(CUHK)in 1973 and his Ph.D.degree from University College London(with Professor Franz Sondheimer)in 1976.After working at Harvard University as a Postdoctoral Associate with Professor Robert B.Woodward,he returned to University College London to begin his independent research undertaking as Ramsay Memorial Fellow.From 1980 to 1982,Professor Wong did research at Shanghai Inst让ute of Organic Chemistry,The Chinese Academy of Sciences.In 1982,he went back to Hong Kong,and started his career at CUHK his alma mater.He retired in 2018,and is now Emeritus Professor of Chemistry and Research Profess or.
基金supported by National Natural Science Foundation of China(31670179,and 91854201)the Research Grants Council of Hong Kong(Ao E/M-05/12,CUHK14104716,and C4002-17G)to L.J.+1 种基金RGC(CUHK14104716)CUHK Direct Grants(4053143,4053174,4053243)to L.C.
文摘Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post-translational regulation of the activities of transcription factors(TFs) is a dynamic and essential mechanism for plant growth and development. CRL3 BPME3 ligase is a CULLIN3-based E3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over-expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106-1 mutants, including Flowering Locus T(FT) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays(EMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction(ChIP-q PCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3 BPME3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3 BPM E3 ligases in Arabidopsis.
