Flavonoids of Rosa rugosa Thunb.inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Rosa rugosa Thunb.is recognized as both medicine and edible in China.The article investigated the antitumor activity of rose flavonoids.Water-extracted rose flavonoids(RFW)and ethanol-extracted rose flavonoids(RFE)were achieved by extracting with distilled water and 70%ethanol,respectively.The effects of the two extracts on proliferation inhibition,apoptosis inducement and metastasis prevention of human HepG2 hepatocellular carcinoma cell lines were tested,via optical/fluorescence microscopy,MTT detection,Transwell assay,flow cytometry and Western blot,etc.The results indicated that rose flavonoids at low concentration(10-40μg/mL)had a better inhibitory effect on migration and invasion of HepG2 cells in a dose-dependent manner,while rose flavonoids at high concentration(80-160μg/mL)could induce apoptosis of HepG2 cells by up-regulating the expression of pro-apoptotic proteins p53 and Bax,and down-regulating the expression of anti-apoptotic proteins Bcl-2,leading to the functioning of caspase-3 and caspase-9.The effect of RFE at the same concentration was significantly better than that of RFW.Conclusion,this study found that rose flavonoids had a certain inhibitory effect on proliferation and metastasis of human liver cancer cells HepG2,indicating the application of rose flavonoids in preventing and treating of liver cancer.

Selective adsorption-involved formation of NMC532/PANI microparticles with high ageing resistance and improved electrochemical performance

Surface modification offers an alternative strategy to improve both ageing resistance and electrochemical performance of cathode materials for lithium-ion batteries.From the viewpoint of real application,surface modification of the cathode materials should be designed with scientificity,effectiveness,low cost,less Li+leaching,and remained tap density.In this contribution,a selective adsorption-involved in-situ growth of polyaniline(PANI)nanoparticles on LiNi_(0.5)Mn_(0.3)Co_(0.2)O_(2)(NMC532)has been designed through a room-temperature-and-pressure chemical vapor deposition technique.The selective growth of PANTI on NMC532 is based on theoretical computation results that multivalent Ni,Mn,and Co are capable of specifically conjugating and activating aniline molecules and,hence,initiating in-situ oxidation polymerization.With only trace amount of aniline monomer,the resulting PANI nanoparticles-inlaid NMC532 microparticles can endure four-month ageing in ambient atmosphere and exhibit improved electrochemical performance at both room temperature and 55℃ compared with pristine NMC532.The improved electrochemical performance of NMC532/PANI is attributed to the enhanced structural stability of NMC532 and inhibited side reactions related to Li_(2)CO_(3) formation,PVDF degradation,electrolyte decomposition,and transition-metal dissolution,owing to PANI modification.

Cloning and Sequence Analysis of Mutant OmpF from Antibiotic Resistant Escherichia coli

[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF(mOmpF)from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into the expression vector pET28a.Subsequently,the expression vector pET28-mOmpF was sequenced and analyzed by DNAMAN software and Swiss-Model online.[Result]Sequence analysis revealed that the open reading fragment of mOmpF was 903 bp long,which was mutated dramatically compared to that of the 1 020 bp long model OmpF.The DNA sequence shared only54.5%homology with OmpF.mOmpF was 44.6%identical to that of OmpF.Protein structure predication and analysis through Swiss-Model online suggested that the structure of mOmpF changed dramatically compared to OmpF.[Conclusion]The present study provided basis for further analyzing the relationships between the structure and functions of mOmpF from antibiotic resistant E.coli.

Syntheses, Characterization and X-ray Crystal Structures of Diiron Ethanedithiolate Complexes Fe_2(S_2C_2H_4 )(CO)_5 (2-Ph_2PC_6H_4NH_2 ) and Fe_2 (S_2C_2H_4 )(CO)_5 (2-Ph_2PC_6H_4CH_2NH_2)

Two new diiron ethanedithiolate complexes Fe2(S2C2H4)(CO)5 (2-Ph2PC6H4NH2) (1) and Fe2(S2C2H4)(CO)5 (2-Ph2PC6H4CH2NH2) (2) as active site models of [FeFe] hydrogenases have been prepared by the treatment of (μ-SCH2CH2 S-μ)Fe2(CO)6 with 2-Ph2PC6H4NH2 or 2-Ph2PC6H4CH2NH2 in the presence of the decarbonylating agent Me3NO·2H2O. As new complexes, both 1 and 2 were fully characterized by elemental analysis, IR and 1H ( 13C, 31P) NMR spectroscopies. In addition, the molecular structure of complex 1 was established by X-ray crystallography. The crystal of Fe2(S2C2H4 )(CO) 5(2-Ph2PC6H4NH2) (1) crystallizes in orthorhombic, spacegroup Pna21 with a = 20.9461(17), b = 13.7615(11), c = 9.3133(7), V = 2684.6(4)3 , Z = 4, C25H20Fe2NO5PS2, Mr = 621.21, Dc = 1.537 g/cm 3 , F(000) = 1264. The final R = 0.0197 and wR = 0.0495 for 4605 observed reflections with I > 2σ(I) and R = 0.0206 and wR = 0.0501 for all data.

TiO_2/NaY Composite as Photocatalyst for Degradation of Omethoate

Photocatalytic activities of TiO2 and NaY zeolites-supported TiO2 were investigated with omethoate as a model pollutant.The physical and chemical states of NaY zeolite-supported TiO2 were evaluated via XRD,FTIR,BET and SEM.Photodegradation of omethoate was studied with H2O2 as oxidant and TiO2 supported on NaY zeolite as photocatalyst.Parameters involved in the photo-catalysis of omethoate,i.e.,the calcination temperature of the photocatalyst,initial omethoate concentration,the amount of TiO2 loaded on NaY zeolite,photocatalyst dosage and H2O2 concentration were investigated in detail.The results show that TiO2/NaY zeolite prepared by means of sol-gel method exhibited a good photocatalytic activity for the degradation of omethoate.Optimum conditions included the calcination temperature of photocatalyst 550 °C,initial omethoate concentration 500 mg/L,the amount of TiO2 loaded on NaY zeolite 35%(mass fraction),the amount of photocatalyst 5 g/L,H2O2 concentration 30 mL/L and an irradiation time of 180 min.The removal of omethoate was up to 93%.Kinetics parameters of the photocatalytic degradation of omethoate were measured and calculated.The result shows the kinetics of photocatalytic degradation of omethoate is first-order.

Theoretical Study on the Mechanism of VO_2^+ + H_2 Reaction in the Gas Phase

The mechanism of VO2+ + H2 reaction in the gas phase was investigated by using density functional theory (DFT) at the CCSD//B3LYP/6-311G(2d, p) level. According to our calculation results, the different reaction mechanisms were found for the singlet and triplet potential energy surfaces (PESs). Especially, the crossing points (CPs) among different PESs were located by means of the intrinsic reaction coordinate (IRC) approach presented by Yoshizawa et al., and the structures and energies of the corresponding minimum energy crossing points (MECPs) were obtained by the mathematical algorithm proposed by Harvey et al. Finally, the frontier molecular orbital (FMO) interaction analyses about MECP1 and MECP2 were used to prove our calculation results.

Optimization of Fermentation Process for the Production of Bacteriocins from Lactic Acid Bacteria

[Objective]This study aimed to improve the yield of bacteriocins from lactic acid bacteria by optimizing the fermentation process for production of bacteriocins from lactic acid bacteria.[Method]By single-factor analysis,fermentation temperature,seed age,inoculation volume,fermentation duration and fermentation media p H were optimized to determine the best fermentation process.The inhibitory zone of bacteriocins from lactic acid bacteria was analyzed with oxford cup method,based on which the fermentation process was evaluated.[Result]The optimal fermentation process was optimized:fermentation temperature 37℃,seed age 14 h,inoculation volume 2%,fermentation duration 48 h,fermentation media p H 5.0.[Conclusion]Under the optimized fermentation conditions,the yield of bacteriocins from lactic acid bacteria was improved significantly.

Mechanisms of MYB-bHLH-WD40 Complex in the Regulation of Anthocyanin Biosynthesis in Plants

Anthocyanins are main coloring substances in plants with various functions such as antioxidant,preventing cardiovascular diseases,and inhibiting oncogenesis. The regulation of transcriptional levels plays a decisive role in the biosynthesis of anthocyanins. Studies have shown that the transcriptional levels of anthocyanins are mainly regulated by MYB-b HLH-WD40 ternary complex. This paper summarized the structure characteristics of MYB-b HLH-WD40 ternary complex and its regulatory role in anthocyanin biosynthesis pathway,focusing on the regulation mechanism of several important model plants by MYB-b HLH-WD40 complex.

Analysis on the Stability of Dendrobium Polysaccharides in Simulated Beverages

[Objective]This study aimed to investigate the stability of Dendrobium polysaccharides in simulated beverages,thus providing theoretical basis for further development of Dendrobium beverages. [Method] The prepared simulated Dendrobium juice beverages and extracted Dendrobium polysaccharides were added into wine and Chinese liquor and treated under different p H,temperature and light conditions to determine changes in Dendrobium polysaccharide content. [Result] Dendrobium polysaccharides exhibited high stability in beverages under neutral p H,room temperature,dark conditions. [Conclusion] Dendrobium polysaccharides can be used for the development of Dendrobium beverages.

Optimization of Fermentation Process for Hyaluronic Acid Production by Streptococcus zooepidemicus

Hyaluronic acid(HA) is a high molecular weight glycosaminoglycan consisting of alternating D-glucuronic acid and N-acetylglucosamine and plays extremely important roles in many biological processes.In this study,we optimized fermentation process for the production of HA by Streptococcus zooepidemicus ATCC35246,including fermentation broth composition and various fermentation parameters.The experimental results showed that the optimal fermentation broth composition was:glucose 45 g/L,yeast extract 10 g/L,tryptone 12 g/L,KH_2PO_42 g/L,K_2HPO_4·3H_2O 2 g/L,MgSO_4 ·7H_2O2 g/L,and(NH_4)_2SO_40.4g/L.The optimal parameters involved in fermentation was:liquid volume 20%,pH 6.0,rotation speed 180 r/min,fermentation temperature 35 °C,fermentation duration 18 h,CTAB concentration 25 mg/L.Under the optimized conditions,the yield of HA was 0.305 g/L,which was dramatically improved by 43.87%compared to that of 0.212 g/L before optimization.

Comparative Study of Variable Selection Using Genetic Algorithm with Various Types of Chromosomes

In this study,different methods of variable selection using the multilinear step-wise regression(MLR) and support vector regression(SVR) have been compared when the performance of genetic algorithms(GAs) using various types of chromosomes is used.The first method is a GA with binary chromosome(GA-BC) and the other is a GA with a fixed-length character chromosome(GA-FCC).The overall prediction accuracy for the training set by means of 7-fold cross-validation was tested.All the regression models were evaluated by the test set.The poor prediction for the test set illustrates that the forward stepwise regression(FSR) model is easier to overfit for the training set.The results using SVR methods showed that the over-fitting could be overcome.Further,the over-fitting would be easier for the GA-BC-SVR method because too many variables fleetly induced into the model.The final optimal model was obtained with good predictive ability(R2 = 0.885,S = 0.469,Rcv2 = 0.700,Scv = 0.757,Rex2 = 0.692,Sex = 0.675) using GA-FCC-SVR method.Our investigation indicates the variable selection method using GA-FCC is the most appropriate for MLR and SVR methods.

Spectral properties of LH2 exhibit very similar even when heterologously express LH2 with <i>β</i>-subunit fusion protein in <i>Rhodobacter</i><i>sphaeroides</i>

Interactions between the light-harvesting subunits and the non-covalently bound photopigments attribute considerably to the spectral properties of photosynthetic bacteria light-harvesting complexes. In our previous studies, we have constructed a novel Rhodobacter sphaeroides expression system. In the present study, we focus on the spectral properties of LH2 when heterologously express LH2 with β-subunit- GFP fusion protein in Rb. sphaeroides. Near infra-red spectrum of LH2 remained nearly unchanged as measured by spectroscopy. Fluorescence spectrum suggested that the LH2 with β-subunit-GFP fusion protein complexes still possessed normal activity in energy transfer. However, photopigments contents were significantly decreased to a very low level in the LH2 with β-subunit-GFP fusion protein complexes compared to that of LH2. FT-IR spectra indicated that interactions between photopigments and LH2 α/β- subunits appeared not to be changed. It was concluded that the LH2 spectral properties exhibited very similar even when heterologously expressed LH2 b-subunit fusion protein in Rb. sphaeroides. Our present study may supply a new insight into better understand the interactions between light-harvesting subunits and photopigments and bacterial photosynthesis and promote the development of the novel Rb. sphaeroides expression system.

Recent advances in multimodal sensing integration and decoupling strategies for tactile perception

Human skin perceives external environmental stimulus by the synergies between the subcutaneous tactile corpuscles.Soft electronics with multiple sensing capabilities by mimicking the function of human skin are of significance in health monitoring and artificial sensation.The last decade has witnessed unprecedented development and convergence between multimodal tactile sensing devices and soft bioelectronics.Despite these advances,traditional flexible electronics achieve multimodal tactile sensing for pressure,strain,temperature,and humidity by integrating monomodal sensing devices together.This strategy results in high energy consumption,limited integration,and complex manufacturing process.Various multimodal sensors and crosstalk-free sensing mechanisms have been proposed to bridge the gap between natural sensory system and artificial perceptual system.In this review,we provide a comprehensive summary of tactile sensing mechanism,integration design principles,signal-decoupling strategies,and current applications for multimodal tactile perception.Finally,we highlight the current challenges and present the future perspectives to promote the development of multimodal tactile perception.

Synthesis and Antitumor Activity Evaluation of γ-Monofluorinated and γγy-Difluorinated Goniothalamin Analogues

一系列小说， -difluorinated Goniothalamin 类似物 4a4i 和 6a6i 被综合。关键步通过脂肪分解酵素 AK 包括了邻近 gem-difluoromethylene 组的 C-5 stereocenter 的构造催化运动分辨率，经由联合的 Stille 的芳基组的介绍，和由 1,5-oxidative 的 lactonization 环合。这些， -difluorinated Goniothalamin 类似物 4a4i 和他们的 enantiomers 6a6i，和几相应 -monofluorinated Goniothalamin，类似物生物学上与四根不同癌症房间线被作比较。复合 7h 在 micromolar 范围作为父母(R)-Goniothalamin 显示出将近相等的力量。在在 antitumor 活动的 fluoromethylene 和 gem-difluoromethylene 之间的不同氟的符号效果通过生物鉴定数据的分析被讨论。

A Mitochondria-Specific Orange/Near-Infrared-Emissive Fluorescent Probe for Dual-Imaging of Viscosity and H_(2)0_(2) in Inflammation and Tumor Models

Elucidating the intrinsic relationship between viscosity/H_(2)0_(2) and mitochondria-associated diseases remains a great challenge owing to the lack of research on multiple diseases models,such as inflammation and malignant tumor models.In this work,we have developed a mitochondria-specific orange/near-infrared-emissive fluorescent probe TTPB,for dual-imaging of viscosity and H_(2)0_(2) levels in two different channels.The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm,and was highly sensitive to H_(2)0_(2) in orange channel with emission peak at 586 nm.Moreover,TTPB has good mitochondria-specific ability and permits individual detecting of viscosity in NIR channels and H_(2)0_(2) levels in orange channel in living cells.More notably,TTPB was successfully applied to simultaneously image the viscosity and H_(2)0_(2) levels in inflammation and cancer models.

Controllable synthesis and photocatalytic activities of rod-shaped mesoporous titanosilicate composites with varied aspect ratios

Rod-shaped mesoporous titanosilicate composites(RMTSs) with controllable aspect ratios(ARs) were fabricated using cetyltrimethylammonium bromide(CTAB) and ammonium hydroxide(NH_4OH) at a continuous stirring rate,resulting in ARs ranging from 1 to 5.Slowing the stirring rate or increasing the concentration of CTAB mainly impacted the length growth,whereas NH_4OH affected the width growth.Photocatalytic activity studies revealed that the length of RMTSs played a more significant role than the width at lower ARs in their photocatalytic activity.

Hybrid Antimicrobial Peptide Buforin Ⅱ-Cecropin B Exhibits Antimicrobial Activity

[Objective] This study aimed to investigate the antimicrobial activity of hybrid antimicrobial peptide buforin II-cecropin B. [Method] Gene fragment BC encoding the hybrid antimicrobial peptide Buforin II-Cecropin B was synthesized by SOE-PCR with six primers designed according to the published amino acid sequences. Then,the BC gene fragment was ligated into p ET32 a vector and expressed in BL21. Antimicrobial ability of the crude hybrid antimicrobial peptide was detected with the Oxford cup method. [Result]The BC gene fragment was correctly amplified by SOE-PCR and ligated into p ET32 a vector,to construct the expression vector p ET32-BC. The hybrid antimicrobial peptide buforin II-cecropin B was expressed in BL21 after induction by IPTG. The optimal induction time was 2 h.Antimicrobial activity assay suggested that the hybrid antimicrobial peptide possessed antimicrobial activity to Escherichia coli and Bacillus subtilis. [Conclusion]The hybrid antimicrobial peptide buforin II-cecropin B exhibited antimicrobial activity to Gram-negative E. coli and Gram-positive B. subtilis strains.

Mosaic structure ZnO formed by secondary crystallization with enhanced photocatalytic performance

Zinc acetate is used as a raw material to synthesize the desired ZnO in hot solvent by controlling the amount of citric acid(CA)added.Notably,the amount of CA added has a significant relationship with the control of the morphology of ZnO.Spherical ZnO wrapped in nanosheets is synthesized through the secondary crystallization of Zn^(2+).The optical properties of the ZnO sample are tested through the degradation of organic pollutants.Notably,the photocatalytic properties of ZnO vary with the different amounts of CA added.Exposure of the active crystal face increases the photocatalytic activity of ZnO.In addition,the number of defects on the surface of the ZnO sample increases because of its large specific surface area,thus changing the bandgap of ZnO.Therefore,the resulting sample can respond under visible light.

Amino terminus mutant OmpA from an isolated antibiotic resistant <i>Escherichia</i><i>coli</i>still possess resistance to environmental stresses

Antibiotic resistant Escherichia coli strains are becoming more common recently. OmpA is a very important antigen protein of E. coli, which consists of two separate domains, N-terminal and C-terminal domain. The N-terminal domain contains eight β- barrel regions that plays important roles in the multifaceted functions of OmpA. In the present study, we cloned a mutant OmpA gene from a multi-antibiotic resistant E. coli strain. Sequence analysis indicated that the N-terminal DNA sequence of the mutant OmpA shared 81.05% homology with the modeled OmpA from E. coli K12 and the N-terminal amino acid sequence of the mutant OmpA was 81.22% identical to that of the E. coli K12 OmpA. Moreover, several amino acids located in the β-barrel region were mutated. The mutant OmpA was expressed in BL21 suggested by SDS-PAGE. Resistance to environmental stress assay indicated that the N-terminus mutant OmpA still possessed excellent activities in pH, temperature and osmotic pressure resistance. Our pre- sent study may supply insights into better and deeper understand the relationships between OmpA N-terminal regions and its functions in environmental stress conditions and the mechanisms on antibiotic resistance of E. coli.

Hydrogels totally from inorganic nanosheets and water with mechanical robustness,self-healing,controlled lubrication and anticorrosion

Synthetic hydrogels with attractive mechanical strength and self-healing are particular appealing,in light of their significance and prospects in industrial,engineering and biomimetic fields.Fabricating various mechanically robust and self-healable hydrogels have achieved some successes in using strong covalently bonded organic polymers as building blocks.However,creation of such soft materials entirely building on rigid inorganic components remains greatly challenging,because inorganic materials are usually poorly flexible and processable.In this study,mechanical robustness and self-recovery are successfully integrated into a single-component colloidal hydrogel system of aluminium hydroxide nanosheets(AHNSs).The inorganic colloidal hydrogel gains an excellent elasticity and stiffness,as indicated by its elastic modulus>10 MPa,due to the use of tough AHNS gelator and the formation of long-range ordered lamellar architectures consisting of self-assembled side-to-side or interlaced-stacking NS superstructures.The metastability in internal gel network endows the hydrogel a self-healing efficiency of larger than 100%.The AHNS hydrogel has been demonstrated to be effectively lubricative and anti-corrosive.Its mechanical,tribological and anticorrosion properties can be optimized by tuning its internal NS configuration and salt content.Our study may be a potent replenishment to the scope of materials science and may provide new insights into nanotechnology,colloidal chemistry,green tribology and mechanical engineering.