Tripartite motif 33(TRIM33), a member of the transcription intermediate factor 1(TIF1) family of transcription cofactors,mediates transforming growth factor-beta(TGF-β) signaling through its PHD-Bromo cassette in mes...Tripartite motif 33(TRIM33), a member of the transcription intermediate factor 1(TIF1) family of transcription cofactors,mediates transforming growth factor-beta(TGF-β) signaling through its PHD-Bromo cassette in mesendoderm differentiation during early mouse embryonic development. However, the role of the TRIM33 RING domain in embryonic differentiation is less clear. Here, we report that TRIM33 mediates Wnt signaling by directly regulating the expression of a specific subset of Wnt target genes, and this action is independent of its RING domain. We show that TRIM33 interacts with β-catenin, a central player in Wnt signaling in mouse embryonic stem cells(mESCs). In contrast to previous reports in cancer cell lines, the RING domain does not appear to function as the E3 ligase for β-catenin, since neither knockout nor overexpression of TRIM33 had an effect on β-catenin protein levels in mESCs. Furthermore, we show that although TRIM33 seems to be dispensable for Wnt signaling through a reporter assay, loss of TRIM33 significantly impairs the expression of a subset of Wnt target genes, including Mixl1,in a Wnt signaling-dependent manner. Together, our results indicate that TRIM33 regulates Wnt signaling independent of the E3 ligase activity of its RING domain for β-catenin in mESCs.展开更多
In the present work, we report the studies concerning liquid-liquid-solid equilibria for the ternary system sodium thiosulphate （Na2S203）＋t-butanol＋water at ambient pressure and at room temperature （303±2 K...In the present work, we report the studies concerning liquid-liquid-solid equilibria for the ternary system sodium thiosulphate （Na2S203）＋t-butanol＋water at ambient pressure and at room temperature （303±2 K）. The solubility data of Na2S203 are reported for solutions in water, t-butanol and solutions of varying concentrations of t-butanol in water. The phase diagram for the said system is developed, described and compared with similar systems studied such as Na2S2O3＋ethanol＋water, K2CO3＋methanol＋water, etc. These results have been explained in terms of structural properties of aqueous t-butanol solutions and further discussed in terms of the effect of ions to cause phase separation.展开更多
基金supported by the National Natural Science Foundation of China (20141300584, 20151301548 to Qiaoran Xi)the THU-PKU Center for Life Sciences
文摘Tripartite motif 33(TRIM33), a member of the transcription intermediate factor 1(TIF1) family of transcription cofactors,mediates transforming growth factor-beta(TGF-β) signaling through its PHD-Bromo cassette in mesendoderm differentiation during early mouse embryonic development. However, the role of the TRIM33 RING domain in embryonic differentiation is less clear. Here, we report that TRIM33 mediates Wnt signaling by directly regulating the expression of a specific subset of Wnt target genes, and this action is independent of its RING domain. We show that TRIM33 interacts with β-catenin, a central player in Wnt signaling in mouse embryonic stem cells(mESCs). In contrast to previous reports in cancer cell lines, the RING domain does not appear to function as the E3 ligase for β-catenin, since neither knockout nor overexpression of TRIM33 had an effect on β-catenin protein levels in mESCs. Furthermore, we show that although TRIM33 seems to be dispensable for Wnt signaling through a reporter assay, loss of TRIM33 significantly impairs the expression of a subset of Wnt target genes, including Mixl1,in a Wnt signaling-dependent manner. Together, our results indicate that TRIM33 regulates Wnt signaling independent of the E3 ligase activity of its RING domain for β-catenin in mESCs.
文摘In the present work, we report the studies concerning liquid-liquid-solid equilibria for the ternary system sodium thiosulphate （Na2S203）＋t-butanol＋water at ambient pressure and at room temperature （303±2 K）. The solubility data of Na2S203 are reported for solutions in water, t-butanol and solutions of varying concentrations of t-butanol in water. The phase diagram for the said system is developed, described and compared with similar systems studied such as Na2S2O3＋ethanol＋water, K2CO3＋methanol＋water, etc. These results have been explained in terms of structural properties of aqueous t-butanol solutions and further discussed in terms of the effect of ions to cause phase separation.
