Identification and Expression Profile of a Neuropeptide LFRFamide-Like Gene During Different Stages of Gonadal Development in the Cephalopod Sepia pharaonis

Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.

Data Quality Control Method of a New Drifting Observation Technology Named Drifting Air-Sea Interface Buoy

An integral quality control(QC)procedure that integrates various QC methods and considers the design indexes and operational status of the instruments for the observations of drifting air-sea interface buoy was developed in the order of basic in-spection followed by targeted QC.The innovative method of combining a moving Hampel filter and local anomaly detection com-plies with statistical laws and physical processes,which guarantees the QC performance of meteorological variables.Two sets of observation data were used to verify the applicability and effectiveness of the QC procedure,and the effect was evaluated using the observations of the Kuroshio Extension Observatory buoy as the reference.The results showed that the outliers in the time series can be correctly identified and processed,and the quality of data improved significantly.The linear correlation between the quality-controlled observations and the reference increased,and the difference decreased.The correlation coefficient of wind speed before and after QC increased from 0.77 to 0.82,and the maximum absolute error decreased by approximately 2.8ms^(-1).In addition,air pressure and relative humidity were optimized by 10^(-3)–10^(-2) orders of magnitude.For the sea surface temperature,the weight of coefficients of the continuity test algorithm was optimized based on the sea area of data acquisition,which effectively expanded the applicability of the algorithm.

Inhibition of Aspergillus parasiticus and Cancer Cells by Marine Actinomycete Strains

Ten actinomycete strains isolated from the Yellow Sea off China＇s coasts were identified as belonging to two genera by 16S rDNA phylogenetic analysis： Streptomyces and Nocardiopsis. Six Streptomyces strains （MA10, 2SHXF01-3, MA35, MA05-2, MA05-2-1 and MA08-1） and one Nocardiopsis strain （MA03） were predicted to have the potential to produce aromatic polyketides based on the analysis of the KSa （ketoacyl-synthase） gene in the type II PKS （polyketides synthase） gene cluster. Four strains （MA03, MA01, MA10 and MA05-2） exhibited significant inhibitory effects on mycelia growth （inhibition rate 〉50%） and subsequent aria- toxin production （inhibition rate 〉75%） of the mutant aflatoxigenic Aspergillus parasiticus NFRI-95. The ethyl acetate extracts of the broth of these four strains displayed significant inhibitory effects on mycelia growth, and the IC50 values were calculated （MA03： 0.275 mg mL-1, MA01：0.106 mg mL-1, MA10：1.345 mg mL-1 and MA05-2：1.362 mg mL-1）. Five strains （2SHXF01-3, MA03, MA05-2, MA01 and MA08-1） were selected based on their high cytotoxic activities. The ethyl acetate extract of the Nocardiopsis strain MA03 was particularly noted for its high antitumor activity against human carcinomas of the cervix （HeLa）, lung （A549）, kidney （Caki-1） and liver （HepG2） （IC50： 2.890, 1.981, 3.032 and 2.603 μgmL-1, respectively）. The extract also remarkably inhibited colony formation of HeLa cells at an extremely low concentration （0.5μgmL 1）. This study highlights that marine-derived actinomycetes are a huge resource of compounds for the biological control of aflatoxin contamination and the development of novel drugs for human carcinomas.

Analysis of characteristics and mechanism of current system on the west coast of Guangdong of China in summer

On the basis of data of drifting bottles＇ tracks and the current measured in anchored stations, as well as temperature and salinity observed in cruise investigations and coastal stations, ADCP current data and AVHRR surface sea temperature （SST） data on the western coast of Guangdong, synthetic results of analysis showed that the coastal currents in the west of the mouth of the Zhujiang River were mainly westward in summer, which constituted the north branch of cyclonic gyre in the east of the Qiongzhou Straits. Part of its water flowed westward into the Beibu Gulf through the Qiongzhou Straits. The coastal current pattern was not identical with the traditional current system which flowed westward in the Qiongzhou Straits in winter and eastward in summer. The summertime＇s coastal current was always westward, maybe temporarily turning northeast only when the southwest wind was strong. The important characteristics of coastal current on the western coast of Guangdong, in the Qiongzhou Straits and in the north of the Beibu Gulf were analyzed and their mechanisms also were explained.

Modeling and Testing of Hydrodynamic Damping Model for a Complex-shaped Remotely-operated Vehicle for Control

In this paper,numerical modeling and model testing of a complex-shaped remotely-operated vehicle（ROV） were shown.The paper emphasized the systematic modeling of hydrodynamic damping using the computational fluid dynamic software ANSYS-CFXTM on the complex-shaped ROV,a practice that is not commonly applied.For initial design and prototype testing during the developmental stage,small-scale testing using a free-decaying experiment was used to verify the theoretical models obtained from ANSYS-CFXTM.Simulation results are shown to coincide with the experimental tests.The proposed method could determine the hydrodynamic damping coefficients of the ROV.

Hydrodynamic Characteristics and Supercavity Shape of Supercavitating Projectiles Launched with Supersonic Speed

A supercavitating projectile is launched underwater with supersonic speed,and then,the speed decreases to transonic and subsonic conditions orderly because of the drag coming from surrounding water.The flow regime and hydrodynamic characteristics are significantly influenced by the flying speed,the influence laws in supersonic,transonic,and subsonic regions are totally different.These issues aren’t well studied.A numerical model consisting of VOF model,moving frame method and state equation of liquid is established to calculate the compressible supercavitation flow field,and validated by comparing with a published result.The influences of water compressibility and Mach number on supercavity shape and hydrodynamic characteristics are quantitatively summarized.The results show that the flying speed of supercavitating projectiles exerts significant influences on the flow regime,supercavity shape and hydrodynamic characteristics for the transonic and supersonic conditions.With the decrease of flying speed,the drag coefficient decreases gradually,and the dimensions of the supercavity near supercavitating projectiles significantly increases in the high-speed conditions.An underwater bow shock is numerically observed before the disk cavitator in supersonic condition.However,no obvious changes are found for the incompressible water cases with different speeds.For supersonic conditions,the supercavity near supercavitating projectiles of compressible water is smaller than that of incompressible water,the drag coefficient is larger,and the relative difference significantly increases with the flying speed.For the case of Ma 1.214,the relative difference of supercavity diameter at the tail section 3.98%,and the difference of the drag coefficient is 23.90%.

Identification of heavy metal-contaminated Tegillarca granosa using laser-induced breakdown spectroscopy and linear regression for classification

Tegillarca granosa(T.granosa)is susceptible to heavy metals,which may pose a threat to consumer health.Thus,healthy and polluted T.granosa should be distinguished quickly.This study aimed to rapidly identify heavy metal pollution by using laser-induced breakdown spectroscopy(LIBS)coupled with linear regression classification(LRC).Five types of T.granosa were studied,namely,Cd-,Zn-,Pb-contaminated,mixed contaminated,and control samples.Threshold method was applied to extract the significant variables from LIBS spectra.Then,LRC was used to classify the different types of T.granosa.Other classification models and feature selection methods were used for comparison.LRC was the best model,achieving an accuracy of 90.67%.Results indicated that LIBS combined with LRC is effective and feasible for T.granosa heavy metal detection.

Antagonizing Aspergillus parasiticus and Promoting Peanut Growth of Bacillus Isolated from Peanut Geocarposphere Soil

A set of 38 Bacillus strains isolated from peanut geocarposphere soil were screened as potential biological control agent anti-Aspergillus parasiticus. Tip-culture method with rapid and quantitative characteristics was used to determine anti-A. parasiticus activity and the process of isolation could be accelerated with this method. 12 out of 38 Bacillus strains showed high anti-alfatoxin production activity. These 12 Bacillus strains were selected to identify the characteristics of promoting peanuts germination rate. Pot experiment in greenhouse was carried out by using these strains which can promote peanuts germination. Phytohormones in the fermentation broth were also detected as another important reference factor to select the isolates as biological control agent with PGPR features. These Bacillus strains isolated from peanut geocarposphere soil not only had high ability anti-Aspergillus parasiticus, but also promoted peanut growth. Therefore, these Bacillus strains were well adapted to peanut production in the ifeld as biological control agent with plant growth promoting rhizobacteria （PGPR） features.

Range estimation of few-shot underwater sound source in shallow water based on transfer learning and residual CNN

Taking the real part and the imaginary part of complex sound pressure of the sound field as features,a transfer learning model is constructed.Based on the pre-training of a large amount of underwater acoustic data in the preselected sea area using the convolutional neural network(CNN),the few-shot underwater acoustic data in the test sea area are retrained to study the underwater sound source ranging problem.The S5 voyage data of SWellEX-96 experiment is used to verify the proposed method,realize the range estimation for the shallow source in the experiment,and compare the range estimation performance of the underwater target sound source of four methods:matched field processing(MFP),generalized regression neural network(GRNN),traditional CNN,and transfer learning.Experimental data processing results show that the transfer learning model based on residual CNN can effectively realize range estimation in few-shot scenes,and the estimation performance is remarkably better than that of other methods.

Buckling of composite cylindrical shells with ovality and thickness variation subjected to hydrostatic pressure

The initial geometric imperfection is one of the primary factors affecting the buckling behaviors of composite cylindrical shells under hydrostatic pressure.In this study,ovality and thickness variations as two representative types of the geometric imperfections are considered.After measuring the geometric imperfections,a typical carbon fiber reinforced polymers(CFRP)cylindrical shell is tested to obtain the buckling pressure.The buckling behaviors of the shell sample are analyzed in combination with the strain responses.By using the nonlinear numerical analysis,the buckling shapes of the CFRP cylinder shells with different combinations of ovality and thickness variation are firstly discussed.The rules of influence of such imperfections on the buckling pressure are then obtained by nonlinear regression method.Finally,an empirical formula is proposed to predict the buckling pressure of the composite cylinder shells,and the calculated results from the formula are in good agreement with the numerical results.

Study on vibration of dragon wash basin and free surface waves inside

We conducted experimental and numerical studies on the vibrating modes of a dragon wash basin (DWB) and the free surface waves inside the DWB. Both the vibration of the DWB and the sound produced were studied carefully. It was found that the DWB can be excited at different intrinsic modes under different excitation, including striking and rubbing it fast/slowly. However, with gentle rubbing, the DWB will be excited mainly at the first vibrating mode. We showed that the concave side wall of a DWB decreases the intrinsic frequencies (compared with a straight side wall), and the ears of a DWB lead to breaking of the vibrating-axisymmetry and cause the separation of modes I/II and IV/V as well. A theoretical model, in which the water is assumed to be incompressible and inviscid, is applied to the first order vibration and predicts the relation of vibrating frequency versus water depth in the system. The measurement shows that both the radial and azimuthal waves are produced as DWB is working. The frequency of the first component of the surface wave is twice as large as the second one induced by non-linear effects. For both the radial and azimuthal waves, the dispersion relation is presented in the framework of capillary wave theory.

Modeling and Simulation of aCounter-Rotating Turbine System for Underwater Vehicles

The structure of a counter-rotating turbine of an underwater vehicle is designed by adding the counter-rotating second-stage turbine disk to the conventional single-stage turbine. The available kinetic energy and the absorption power of the auxiliary system are calculated at different working conditions, and the results show that the power of the main engine and auxiliary system at the counter-rotating turbine system matches well with each other. The technology scheme of the counter-rotating turbine system is proposed, then the experimental simulation of the lubricating oil loop, fuel loop, and seawater loop is completed. The simulation results indicate that the hydraulic transmission system can satisfy the requirements for an underwater vehicle running at a steady sailing or variable working conditions.

Straight-Path Following and Formation Control of USVs Using Distributed Deep Reinforcement Learning and Adaptive Neural Network

Dear Editor,This letter presents a distributed deep reinforcement learning(DRL) based approach to deal with the path following and formation control problems for underactuated unmanned surface vehicles(USVs). By constructing two independent actor-critic architectures,the deep deterministic policy gradient(DDPG) method is proposed to determine the desired heading and speed command for each USV.

Spatiotemporal distributions and environmental drivers of diversity and community structure of nosZ-type denitrifiers and anammox bacteria in sediments of the Bohai Sea and North Yellow Sea, China

Denitrification and anammox processes are major nitrogen removal processes in coastal ecosystems. However, the spatiotemporal dynamics and driving factors of the diversity and community structure of involved functional bacteria have not been well illustrated in coastal environments, especially in human-dominated ecosystems. In this study, we investigated the distributions of denitrifiers and anammox bacteria in the eutrophic Bohai Sea and the northern Yellow Sea of China in May and November of 2012 by constructing clone libraries employing nosZ and 16S rRNA gene biomarkers. The diversity of nosZ-denitrifier was much higher at the coastal sites compared with the central sites, but not significant among basins or seasons. Alphaproteobacteria were predominant and prevalent in the sediments, whereas Betaproteobacteria primarily occurred at the site near the Huanghe (Yellow) River estuary. Anammox bacteria Candidatus Scalindua was predominant in the sediments, and besides, Candidatus Brocadia and Candidatus Kuenenia were also detected at the site near the Huanghe River estuary that received strong riverine and anthropogenic impacts. Salinity was the most important in structuring communities of nosZ-denitrifier and anammox bacteria. Additionally, anthropogenic perturbations (e.g. nitrogen overloading and consequent high primary productivity, and heavy metal discharges) contributed significantly to shaping community structures of denitrifier and anammox bacteria, suggesting that anthropogenic activities would influence and even change the ecological function of coastal ecosystems.

Statistical characteristics and mechanisms of mesoscale eddies in the North Indian Ocean

The statistical characteristics and mechanisms of mesoscale eddies in the North Indian Ocean are investigated by adopting multi-sensor satellite data from 1993 to 2019.In the Arabian Sea(AS),seasonal variation of eddy characteristics is remarkable,while the intraseasonal variability caused by planetary waves is crucial in the Bay of Bengal(BOB).Seasonal variation of the eddy kinetic energy(EKE)is distinct along the west boundary of AS,especially in the Somali Current region.In the BOB,larger EKE occurs at the northwest basin from March to May,to the east of Sri Lanka from June to September,and along the east coast of India from November to December.The wind stress work(WW)is further studied to figure out the direct influence of wind forcing on EKE.The WW exerts positive effects on EKE along the west boundary of AS and in the south of India/Sri Lanka during the two monsoon seasons.Besides,the WW also has impact on EKE along the east coast of India in November and December.Eventually,we investigate the characteristics and the driving mechanisms of long lifespan eddies.In the AS,long lifespan anti-cyclonic eddies(AEs)mainly generate in the Socotra,the West Indian Coastal Current and the East Arabian Current regions,while cyclonic eddies(CEs)are concentrated in the northwest region.In the BOB,long lifespan AEs mostly form near the west of Myanmar,while CEs are accumulated at the north and northwest basin.The instabilities caused by Rossby waves,coastal Kelvin waves,seasonal currents,together with wind stress forcing exert enormous efforts on the generation and evolution of these eddies.

Observation of an anti-cyclonic mesoscale eddy in the subtropical northwestern Pacific Ocean from altimetry and Argo profiling floats

The comprehensive three-dimensional structures of an anti-cyclonic mesoscale eddy(AE)in the subtropical northwestern Pacific Ocean were investigated by combining the Argo floats profiles with enhanced vertical and temporal sampling and satellite altimetry data.The AE originated near the Kuroshio Extension and then propagated westward with mean velocity of 8.9 cm/s.Significant changes and evolutions during the AE’s growing stage(T1)and further growing stage(T2)were revealed through composite analysis.In the composite eddy core,maximum temperature(T)and salinity(S)anomalies were of 1.7(1.9)℃ and 0.04(0.07)psu in T1(T2)period,respectively.The composite T anomalies showed positive in almost whole depth,but the S anomalies exhibited a sandwich-like pattern.The eddy’s intensification and its influence on the intermediate ocean became more significant during its growth.The trapping depth increased from 400×10^4 Pa to 580×10^4 Pa while it was growing up,which means more water volume,heat and salt content in deeper layers can be transported.The AE was strongly nonlinear in upper oceans and can yield a typical mean volume transport of 0.17×10^6 m^3/s and a mean heat and salt transport anomaly of 3.6×10^11 W and–2.1×10^3 kg/s during the observation period.The Energy analysis showed that eddy potential and kinetic energy increased notably as it propagated westward and the baroclinic instability is the major energy source of the eddy growth.The variation of the remained Argo float trapped within the eddy indicated significant water advection during the eddy’s propagation.

Influence of Ramjets’ Water Inflow on Supercavity Shape and Cavitator Drag Characteristics

Water ramjets using outer water as an oxidizer have been demonstrated as a potential propulsion mode for underwater High Speed Supercavitating Vehicles (HSSVs) because of their higher energy density, power density, and specific impulse, but water flux changes the shapes of supercavity. To uncover the cavitator drag characteristics and the supercavity shape of HSSVs with water inflow for ramjets, supercavitation flows around a disk cavitator with inlet hole are studied using the homogenous model. By changing the water inflow in the range of 0-10 L/s through cavitators having different water inlet areas, a series of numerical simulations of supercavitation flows was performed. The water inflow flux of ramjets significantly influences the drag features of disk cavitators and the supercavity shape, but it has little influence on the slender ratio of supercavitaty. Furthermore, as the water inlet area increases, the drag coefficient of the cavitators' front face decreases, but this increase does not influence the diameter of the supercavity's maximum cross section and the drag coefficient of the entire cavitator significantly. In addition, with increasing waterflux of the ramjet, both the drag coefficient of cavitators and the maximum diameter of supercavities decrease stably. This research will be helpful for layout optimization and supercavitaty scheme design of HSSVs with water inflow for ramjets.

Rapid changes in the near-bottom temperature of the bottom aquaculture area around the Zhangzi Island in summer

Rapid changes in the near-bottom water temperature are important environmental factors that can significantly affect the growth and development of species in the bottom culture.The object of this research is to investigate the mechanism causing these rapid changes within a bottom culture area near the Zhangzi Island.The hydrographic transects observations in the North Yellow Sea(NYS)suggest that our mooring station is very close to the tidal mixing front.The horizontal advection of the tidal front has induced the observed tidal change of bottom temperature at the mooring station.Analysis of the mooring near-bottom temperature and current measurements show that the angle between the tidal current horizontal advection and the swing of the tidal front is crucial in determining the variation trend of temperature.When the angle equals 90°,the horizontal tidal current advects along the isotherms so the temperature remains the same.When the angle is between 0°and 90°,the seawater moves from deep water to the warmer coastal zone and the temperature decreases.In contrast,the horizontal tidal advection moves the coastal warm water to the mooring station and the water temperature increases when the angle is between 90°and 180°.The amplitude of the temperature change is proportional to the magnitude of the horizontal temperature gradient and the tidal excursion in the direction of the temperature gradient.This study may facilitate the choice of culture area in order to have a good aquaculture production.

Velocity structure in the South Yellow Sea basin based on first-arrival tomography of wide-angle seismic data and its geological implications

The South Yellow Sea basin is filled with Mesozoic-Cenozoic continental sediments overlying pre-Palaeozoic and Mesozoic-Palaeozoic marine sediments.Conventional multi-channel seismic data cannot describe the velocity structure of the marine residual basin in detail,leading to the lack of a deeper understanding of the distribution and lithology owing to strong energy shielding on the top interface of marine sediments.In this study,we present seismic tomography data from ocean bottom seismographs that describe the NEE-trending velocity distributions of the basin.The results indicate that strong velocity variations occur at shallow crustal levels.Horizontal velocity bodies show good correlation with surface geological features,and multi-layer features exist in the vertical velocity framework(depth:0–10 km).The analyses of the velocity model,gravity data,magnetic data,multichannel seismic profiles,and drilling data showed that high-velocity anomalies(>6.5 km/s)of small(thickness:1–2 km)and large(thickness:>5 km)scales were caused by igneous complexes in the multi-layer structure,which were active during the Palaeogene.Possible locations of good Mesozoic and Palaeozoic marine strata are limited to the Central Uplift and the western part of the Northern Depression along the wide-angle ocean bottom seismograph array.Following the Indosinian movement,a strong compression existed in the Northern Depression during the extensional phase that caused the formation of folds in the middle of the survey line.This study is useful for reconstructing the regional tectonic evolution and delineating the distribution of the marine residual basin in the South Yellow Sea basin.

Inhibition of norsolorinic acid accumulation to Aspergillus parasiticus by marine actinomycetes

Thirty-six strains of marine actinomycetes were isolated from a sample of marine sediment collected from the Yellow Sea and evaluated in terms of their inhibitory activity on the growth of Aspergillus parasiticus and the production of norsolorinic acid using dual culture plate assay and agar diffusion methods.Among them,three strains showed strong antifungal activity and were subsequently identified as Streptomyces sp.by 16 S rRNA gene sequencing analysis.The supernatant from the fermentation of the MA01 strain was extracted sequentially with chloroform and ethyl acetate,and the activities of the extracts were determined by tip culture assay.The assay results show that both extracts inhibited mycelium growth and toxin production,and the inhibitory activities of the extracts increased as their concentrations increased.The results of this study suggest that marine actinomycetes are biologically important for the control of mycotoxins,and that these bacteria could be used as novel biopesticides against mycotoxins.