We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus, and compared the response of screened and unscreened (control) P...We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus, and compared the response of screened and unscreened (control) P. trituberculatus challenged with V. alginolyticus. We measured superoxide dismutase, catalase, acid phosphatase, alkaline phosphatase, and peroxidase activity and the content of hemocyanin in the plasma and phenoloxidase activity in serum. The cumulative survival rate after 24-h challenge with V. alginolyticus was significantly higher in the screened crabs than in the unscreened crabs (P<0.05). T-SOD and PO activity were significantly lower in the screened stock than in the unscreened stock (P<0.05). POD, CAT, and ACP activity and hemocyanin content were significantly higher in the screened stock than in the unscreened stock. Our results suggest that the screened stock was more resistant to infection. Furthermore, the indices we measured may be used to evaluate the health state of P. trituberculatus.展开更多
基金Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A409)the National Natural Science Foundation of China (No. 41106123)+10 种基金the Transformation Fund for Agricultural Science (No. 2010GB2C220537)the Research Fund for the Doctoral Program of Higher Education (No. 20103305120002)the Scientific Research Fund of Zhejiang Provincial Education Department (No.Z201121258)the Zhejiang Key Innovative Team of Marine Aquaculture(No. 2010R50025-08)the Ningbo Natural Science Foundation (No.2010A610004)the Ningbo Innovative Program of Agriculture (No.2011C92014)the Ningbo Special Program of Agriculture (No. 201015)the Ningbo Special Program of Marine Fishing (No. 2011-1-7)the Public Interest Program of Zhejiang Province (No. 2011C33025)the Program for Science and Technology Innovative Research Team of Ningbo (No.2011B81003)the K C Wong Magana Fund in Ningbo University
文摘We established a line (screened) of Portunus trituberculatus by selectively breeding individuals that survived from challenge with Vibrio alginolyticus, and compared the response of screened and unscreened (control) P. trituberculatus challenged with V. alginolyticus. We measured superoxide dismutase, catalase, acid phosphatase, alkaline phosphatase, and peroxidase activity and the content of hemocyanin in the plasma and phenoloxidase activity in serum. The cumulative survival rate after 24-h challenge with V. alginolyticus was significantly higher in the screened crabs than in the unscreened crabs (P<0.05). T-SOD and PO activity were significantly lower in the screened stock than in the unscreened stock (P<0.05). POD, CAT, and ACP activity and hemocyanin content were significantly higher in the screened stock than in the unscreened stock. Our results suggest that the screened stock was more resistant to infection. Furthermore, the indices we measured may be used to evaluate the health state of P. trituberculatus.
