Objective:To characterize the genetic structure of various populations of Culex quinquefasciatus (Cx.quinquefasciatus) from India representing different geoclimatic locations.Methods:Inter simple sequence repeat(ISSR)...Objective:To characterize the genetic structure of various populations of Culex quinquefasciatus (Cx.quinquefasciatus) from India representing different geoclimatic locations.Methods:Inter simple sequence repeat(ISSR) markers were used.A set of 20 primers were screened with the laboratory populations of mosquito species.Finally the IS 40 primer was chosen based on the scorable banding pattern showing 100 percent polymorphism among the various populations.The statistical analysis was done using POPGENE 1.31 software.The consensus tree was generated based on UPGMA modified from NEIGHBOR procedure of PHYLIP Version 3.5.Results:The cluster analysis shows the main cluster which is divided into two sub cluster representing all the populations separated as per their phylogeographic and geoclimatic condition.Conclusions: The findings will be helpful in understanding the population variation under different ecological conditions and development of effective vector management strategies.展开更多
Exposure to mercuric chloride(HgCl2;5 mg kg–1 body weight;i.p.)induced oxidative stress in mice and substantially increased lipid peroxidation(LPO)and oxidized glutathione(GSSG)levels,decreased the level of reduced g...Exposure to mercuric chloride(HgCl2;5 mg kg–1 body weight;i.p.)induced oxidative stress in mice and substantially increased lipid peroxidation(LPO)and oxidized glutathione(GSSG)levels,decreased the level of reduced glu-tathione(GSH)and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum.Therapy with propolis extract,a resinous wax-like beehive product(200 mg kg–1 orally,after mercury administration),for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver.Release of serum transaminases,alkaline phosphatase,lactate dehydrogenase and-glutamyl transpeptidase were significantly restored after propolis treatment.The activities of antioxidant enzymes,that is,superoxide dismutase,catalase,glutathione-S-transferase and glucose-6-phosphate dehydrogenase,were also concomitantly restored towards normal levels after propolis administration.These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury-induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent.展开更多
文摘Objective:To characterize the genetic structure of various populations of Culex quinquefasciatus (Cx.quinquefasciatus) from India representing different geoclimatic locations.Methods:Inter simple sequence repeat(ISSR) markers were used.A set of 20 primers were screened with the laboratory populations of mosquito species.Finally the IS 40 primer was chosen based on the scorable banding pattern showing 100 percent polymorphism among the various populations.The statistical analysis was done using POPGENE 1.31 software.The consensus tree was generated based on UPGMA modified from NEIGHBOR procedure of PHYLIP Version 3.5.Results:The cluster analysis shows the main cluster which is divided into two sub cluster representing all the populations separated as per their phylogeographic and geoclimatic condition.Conclusions: The findings will be helpful in understanding the population variation under different ecological conditions and development of effective vector management strategies.
基金The authors wish to thank the Indian Council of Medical Research,New Delhi,India(45/15/2002/PHA/BMS)for financial assistance.
文摘Exposure to mercuric chloride(HgCl2;5 mg kg–1 body weight;i.p.)induced oxidative stress in mice and substantially increased lipid peroxidation(LPO)and oxidized glutathione(GSSG)levels,decreased the level of reduced glu-tathione(GSH)and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum.Therapy with propolis extract,a resinous wax-like beehive product(200 mg kg–1 orally,after mercury administration),for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver.Release of serum transaminases,alkaline phosphatase,lactate dehydrogenase and-glutamyl transpeptidase were significantly restored after propolis treatment.The activities of antioxidant enzymes,that is,superoxide dismutase,catalase,glutathione-S-transferase and glucose-6-phosphate dehydrogenase,were also concomitantly restored towards normal levels after propolis administration.These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury-induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent.
