The environmental analysis and site selection of mussel and large yellow croaker aquaculture areas based on high resolution remote sensing

Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was proposed and applied to determine the basic environmental characteristics of the best mussel and large yellow croaker aquaculture areas.This methodology includes the first step of extraction of the location distribution and the second step of the extraction of internal environmental factors.The fishery ranching index(FRI1,FRI2)was established to extract the mussel and the large yellow croaker aquaculture area in Zhoushan,using Gaofen-1(GF-1)and Gaofen-6(GF-6)satellite data with a special resolution of 2 m.In the second step,the environmental factors such as sea surface temperature(SST),chlorophyll a(Chl-a)concentration,current and tide,suspended sediment concentration(SSC)in mussel aquaculture area and large yellow croaker aquaculture area were extracted and analyzed in detail.The results show the following three points.(1)For the extraction of the mussel aquaculture area,FRI1 and FRI2 are complementary,and the combination of FRI1 and FRI2 is suitable to extract the mussel aquaculture area.As for the large yellow croaker aquaculture area extraction,FRI2 is suitable.(2)Mussel aquaculture and the large yellow croaker aquaculture area in Zhoushan are mainly located on the side near the islands that are away from the eastern open waters.The water environment factor template suitable for mussel and large yellow croaker aquaculture was determined.(3)This two-step remote sensing method can be used for the preliminary screening of potential site selection for the mussels and large yellow croaker aquaculture area in the future.the fishery ranching index(FRI1,FRI2)in this paper can be applied to extract the mussel and large yellow croaker aquaculture areas in coastal waters around the world.

Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis

BACKGROUND Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology.Alkaline sphingomyelinase(alk-SMase)is specifically expressed by intestinal epithelial cells,and has been reported to play an anti-inflammatory role.However,the underlying mechanism is still unclear.AIM To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium(DSS)-induced colitis.METHODS Mice were administered 3%DSS drinking water,and disease activity index was determined to evaluate the status of colitis.Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran,and bacterial translocation was evaluated by measuring serum lipopolysaccharide.Intestinal epithelial cell ultrastructure was observed by electron microscopy.Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA,respectively.Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels.RESULTS Compared to wild-type(WT)mice,inflammation and intestinal permeability in alk-SMase knockout(KO)mice were more severe beginning 4 d after DSS induction.The mRNA and protein levels of intestinal barrier proteins,including zonula occludens-1,occludin,claudin-3,claudin-5,claudin-8,mucin 2,and secretory immunoglobulin A,were significantly reduced on 4 d after DSS treatment.Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells.Furthermore,by day 4,mitochondria appeared swollen and degenerated.Additionally,compared to WT mice,serum malondialdehyde levels in KO mice were higher,and the antioxidant capacity was significantly lower.The expression of the transcription factor nuclear factor erythroid 2-related factor 2(Nrf2)in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment.mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased.Finally,colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone,which is an Nrf2 activator.CONCLUSION Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.

Comprehensive understanding of the thriving electrocatalytic nitrate/nitrite reduction to ammonia under ambient conditions

Ammonia(NH_(3))is a multifunctional compound that is an important feedstock for the agricultural and pharmaceutical industries and attractive energy storage medium.At present,NH_(3)synthesis is highly dependent on the conventional Haber–Bosch process that operates under harsh conditions,which consumes large quantities of fossil fuels and releases a large amount of carbon dioxide.As an alternative,electrosynthesis is a prospective method for producing NH_(3)under normal temperature and pressure conditions.Although electrocatalytic nitrogen reduction to ammonia has attracted considerable attentions,the low solubility of N_(2)and high N≡N cracking energy render the achievements of high NH_(3) yield rate and Faradaic efficiency difficult.Nitrate and nitrite(NO_(x)^(-))are common N-containing pollutants.Due to their high solubilities and low dissociation energy of N=O,NO_(x)^(-)−are ideal raw materials for NH_(3) production.Therefore,electrocatalytic NO_(x)^(-)−reduction to NH_(3)(eNO_(x)RR)is a prospective strategy to simultaneously realise environmental protection and NH_(3) synthesis.This review offers a comprehensive understanding of the thriving eNO_(x)RR under ambient conditions.At first,the popular theory and mechanism of eNO_(x)RR and a summary of the measurement system and evaluation criteria are introduced.Thereafter,various strategies for developing NO_(x)−reduction catalysts are systematically presented and discussed.Finally,the challenges and possible prospects of electrocatalytic NO_(x)^(-1) reduction are outlined to facilitate energy-saving and environmentally friendly large-scale synthesis of NH_(3) in the future.

RPL-Based IoT Networks under Decreased Rank Attack:Performance Analysis in Static and Mobile Environments

The RPL(IPv6 Routing Protocol for Low-Power and Lossy Networks)protocol is essential for efficient communi-cation within the Internet of Things(IoT)ecosystem.Despite its significance,RPL’s susceptibility to attacks remains a concern.This paper presents a comprehensive simulation-based analysis of the RPL protocol’s vulnerability to the decreased rank attack in both static andmobilenetwork environments.We employ the Random Direction Mobility Model(RDM)for mobile scenarios within the Cooja simulator.Our systematic evaluation focuses on critical performance metrics,including Packet Delivery Ratio(PDR),Average End to End Delay(AE2ED),throughput,Expected Transmission Count(ETX),and Average Power Consumption(APC).Our findings illuminate the disruptive impact of this attack on the routing hierarchy,resulting in decreased PDR and throughput,increased AE2ED,ETX,and APC.These results underscore the urgent need for robust security measures to protect RPL-based IoT networks.Furthermore,our study emphasizes the exacerbated impact of the attack in mobile scenarios,highlighting the evolving security requirements of IoT networks.

Analysis of the Effect and Influencing Factors of Rural Domestic Sewage Treatment Based on A^(2)O-MBBR Integrated Process

[Objectives] This study was conducted to solve the prominent problems in the treatment of domestic sewage in southern rural areas of China. [Methods] An integrated process treatment mode of anaerobic/anoxic/aerobic moving bed biofilm reactor (A 2O-MBBR) was proposed to analyze and study its operating effect and influencing factors. [Results] The A^(2)O-MBBR mode had good COD removal efficiency and nitrogen and phosphorus removal performance, and the water quality index of the effluent met the Class A standard of GB181918-2002. This mode is suitable for treating rural domestic sewage, and has high treatment effects in different operating periods. In spring, the average removal rates of COD, NH_(4)^(+)-N, TN, TP and SS reached (83.53 ± 2.15)%, (89.44 ± 4.97)%, (67.36±18.53)%, (88.22±11.21)% and (91.73±2.25)%, respectively;In the autumn period, the average removal rates of COD, NH_(4)^(+)-N, TN, TP and SS were (83.49±2.64)%, (89.26±9.19)%, (66.05±17.00)%, (87.48±9.68)%, and (91.13±2.35)%. [Conclusions] This study provides theoretical reference and technical support for the popularization and application of A^(2)O-MBBR integrated process.

Design and Analysis of Power and Transmission System of Downhole Pure Electric Command Vehicle

Electric vehicles use electric motors, which turn electrical energy into mechanical energy. As electric motors are conventionally used in all the industry, it is an established development site. It’s a mature technology with ideal power and torque curves for vehicular operation. Conventional vehicles use oil and gas as fuel or energy storage. Although they also have an excellent economic impact, the continuous use of oil and gas threatened the world’s reservation of total oil and gas. Also, they emit carbon dioxide and some toxic ingredients through the vehicle’s tailpipe, which causes the greenhouse effect and seriously impacts the environment. So, as an alternative, electric car refers to a green technology of decarbonization with zero emission of greenhouse gases through the tailpipe. So, they can remove the problem of greenhouse gas emissions and solve the world’s remaining non-renewable energy storage problem. Pure electric vehicles (PEV) can be applied in all spheres, but their special implementation can only be seen in downhole operations. They are used for low noise and less pollution in the downhole process. In this study, the basic structure of the pure electric command vehicle is studied, the main components of the command vehicle power system, namely the selection of the drive motor and the power battery, are analyzed, and the main parameters of the drive motor and the power battery are designed and calculated. The checking calculation results show that the power and transmission system developed in this paper meets the design requirements, and the design scheme is feasible and reasonable.

Catalogue of the Type Specimens of Amphibians and Reptiles in the Herpetological Museum of the Chengdu Institute of Biology,Chinese Academy of Sciences:Ⅱ.Hynobiidae (Amphibia,Urodela)

In order to facilitate herpetological research for herpetologists in and outside of China,we will report all the type specimens of Amphibia and Reptilia deposited in the Herpetological Museum of the Chengdu Institute of Biology(CIB),Chinese Academy of Sciences(CAS) in series. This report focuses on the type specimens of the family Hynobiidae of Urodela in Amphibia,which comprises seven species. Each entry provides the genus name,species name,information of the original description,the catalog number of holotype,sex,type locality,information of allotype(if any) and paratype,number and distribution of deposited specimens,and comments where they are appropriate.

Numerical Simulation and Reinforcing Technology Research on Collapse of Weak Interlayer Shaft

The three-level shaft of the mixed pit shaft of Yixin Mine,built in 1983,failed continuously due to the influence of geological structure and the effect of water,which formed a collapsed space with a length of 18m,a depth of 12m,and a height of 12m at the level of-255m and to the southwest end.The 54m pit shaft below the-258m elevation of the pit shaft was filled with collapsed rocks forming a large loose body.The comprehensive processing plan for falling grouting and anchoring was proposed according to the geological conditions of the vertical shaft of the mixed pit shaft,adopting three-dimensional numerical simulation software as well as calculating the mechanical state of the vertical pit shaft surrounding rocks and using 3 to 5 parameters of Williams-Warnke failure criterion.

Comparative mitochondrial genome analysis of Cynoglossidae(Teleost:Pleuronectiformes)and phylogenetic implications

Generally,a teleostean group(e.g.,family or genus)owns one type or a set of similar mitochondrial gene arrangement.It is interesting,however,that four different types of gene arrangement have been found in the mitochondrial genome(mitogenome)of Cynoglossidae species.So far,the possible mechanisms of mitogenomic gene rearrangement and its potential implications have aroused widespread attention and caused lots of controversy.Here,a total of 21 Cynoglossidae mitogenomes and a newly sequenced mitogenome of Cynoglossus puncticpes(Pleuronectiformes:Cynoglossidae)were compared.The length ranges from 16417 bp to 18369 bp,which is mainly caused by the length heteroplasmy of control region(CR).Further analysis reveals that the difference of tandem repeats acts as a determining factor resulting in the length heterogeneity.Like most gene rearrangements of Cynoglossinae mitogenomes,tRNA-Gln gene encoded by the L-strand has translocated to the H-strand(Q inversion),accompanied by the translocation of CR in C.puncticpes mitogenome.The typical IQM order(tRNA-Ile-Gln-Met)changed to QIM order.Tandem duplication/random loss and mitochondrial recombination were accepted as the most possible models to account for the rearrangements in C.puncticpes mitogenome.Phylogenetic trees showed a strong correlation between the gap spacer in the rearranged QIM area and phylogeny,which provides a fresh idea for phylogenetic studies in future.

Two complete mitogenomes of Ocypodoidea(Decapoda: Brachyura), Cleistostoma dilatatum(Camptandriidae) and Euplax sp.(Macrophthalmidae) and its phylogenetic implications

Complete mitochondrial genomes(mitogenomes) can provide useful information for phylogenetic relationships,gene rearrangement, and molecular evolution. In the present study, two newly sequenced mitogenomes of Ocypodoidea(Cleistostoma dilatatum and Euplax sp.) were reported for the first time, which are 15 444 bp and16 129 bp in length, respectively. Cleistostoma dilatatum is the first species in the family Camptandriidae whose complete mitogenome was sequenced. Each mitogenome contains an entire set of 37 genes and a putative control region, but their gene arrangements are largely different. Tandem duplication and random loss model is proposed to account for their gene arrangements. Comparative genomic analyses of 19 mitogenomes clustering in one branch reveal that 18 of them shared the same gene rearrangement, while that of C. dilatatum mitogenome was consistent with the ancestral gene arrangement of Brachyura. The dN/dS ratio analysis shows that all PCGs are evolving under purifying selection. Phylogenetic analyses show that all Macrophalmidae species cluster together as a group, and then form a sister clade with Camptandriidae. Moreover, the polyphyly of three superfamilies(Ocypodoidea, Eriphioidea, and Grapsoidea) is reconfirmed. These findings help to confirm the phylogenetic position of Camptandriidae, as well as provide new insights into the phylogeny of Brachyura.

Study of coexisting upstream solitary wave packet and downstream wakes induced by Diaoyu Dao and its affiliated islands detected by satellite sun glitter

Taking the advantage of sun glitter(SG)observed from high-resolution satellites Gaofen-1(GF-1)and Gaofen-6(GF-6),a new method named Shield and Vortex 1-2(SAV1-2)for extracting spatial texture information from sun glitter was established.Sea surface texture detail information around Diaoyu Dao and its affiliated islands was extracted using SAV1-2.Meanwhile,the dynamic characteristics of the water environment were analyzed by combining the water color,temperature and dynamic data.The results show the following information.(1)Stable westward shield effect and eastward Karman vortex streets,extracted from SG,appear upstream(westward)and downstream(eastward)of the Diaoyu Dao and its affiliated islands respectively.(2)The dominant direction of the Kuroshio Current in the Diaoyu Dao and its affiliated islands area is east,the Diaoyu Dao and its affiliated islands interacting with Kuroshio,inducing western shield and eastern shelter and wake.Furthermore,wave intervals of the shield in front of the island range from hundreds to thousands of metres,and extend wider than the island itself.(3)Combining with high-resolution sea surface temperature(SST)and chlorophyll a(Chl a)concentration,the directional changes of water color elements are mutually supported and proved with the stable shield(wave packet)effect in the west and vortex street effect in the east.The change of SST upstream of the island(western shield area)is not significant,the temperature in the downstream shelter area decreases slightly,and the SST in the downstream wake area shows a general trend of obvious decrease.The wake area is dominated by Karman vortex streets in shape,supplementing by vortices.The concentration of Chl a in the west shield of island is as low as that in downstream shelter area,while it increases significantly in the downstream wake area with shape in eddy or ribbon.(4)The SAV methods of extracting sea surface texture detail using SG can be widely used in different sea areas and water quality.This paper can provide reference for the protection and development of Diaoyu Dao and its affiliated islands and its surrounding waters.It is suggested that some kind of current buffer,such as marine wind farm,should be established in the western waters of Diaoyu Dao and its affiliated islands to protect the islands,and marine pasture should be developed in the downstream of eastern waters of Diaoyu Dao and its affiliated islands.

Development of Pandemic Monitoring System Based on Constellation of Nanosatellites

Covid-19 is a global crisis and the greatest challenge we have faced.It affects people in different ways.Most infected people develop a mild to moderate form of the disease and recover without hospitalization.This presents a problem in spreading the pandemic with unintentionally manner.Thus,this paper provides a new technique for COVID-19 monitoring remotely and in wide range.The system is based on satellite technology that provides a pivotal solution for wireless monitoring.This mission requires a data collection technique which can be based on drones’technology.Therefore,the main objective of our proposal is to develop a mission architecture around satellite technology in order to collect information in wide range,mostly,in areas suffer network coverage.A communication method was developed around a constellation of nanosatellites to cover Saudi Arabia region which is the area of interest in this paper.The new proposed architecture provided an efficient monitoring application discussing the gaps related to thermal imaging data.It reached 15.8 min as mean duration of visibility for the desired area.In total,the system can reach a coverage of 5.8 h/day,allowing to send about 21870 thermal images.

Optimization of Extraction Conditions of Pomegranate Peel Polyphenols and Its Protective Effect on Acute Alcoholic Liver Injury in Mice

[Objectives]This study was conducted to explore the optimization of ultrasonic-assisted organic solvent extraction of pomegranate peel polyphenols(PPPs),and to study the protective effect of PPPs on acute alcoholic liver injury in mice.[Methods]The optimal extraction conditions of PPPs were determined by single factor and orthogonal experiments,and an acute alcoholic liver injury model in mice was established.Bifendate was used as the positive control group to investigate the protective effect of low,medium and high doses of PPPs on acute alcoholic liver injury.[Results]The optimum extraction process parameters were followed as 60%ethanol concentration,solid-liquid ratio of 1:40(w/v),extraction temperature of 50℃,and extraction time of 1.5 h,and the yield was 1.42%.The results of animal experiments showed that PPPs could effectively reduce the degree of alcoholic liver injury in mice,reduce the levels of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and reduce the inflammation and necrosis of liver tissue in mice.Meanwhile,the total polyphenols from pomegranate peel also significantly reduced the expression levels of malondialdehyde(MDA),tumor necrosis factor(TNF-α)and interleukin-6(IL-6)in mice,and increased the levels of superoxide dismutase(SOD)and reduced glutathione(GSH)in liver tissue of mice,indicating its antioxidant and anti-inflammatory effects,further illustrating its protective effect on alcoholic liver injury.[Conclusions]PPPs could reduce the expression levels of TNF-α,IL-6 and MDA in mice,and increase the expression levels of SOD and GSH to achieve the protective effect on acute alcoholic liver injury in mice.This study will provide new ideas for the development of new anti-alcoholic liver injury drug resources.

Optimization of Extraction Conditions for Total Flavonoids from Fructus Aurantii Immaturus and Its Anti-UVB Radiation Activity

[Objectives]Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus(TFFAI)and its resistance activity to ultraviolet radiation were investigated in present research.[Methods]The optimal extraction conditions of TFFAI were determined by single factor and orthogonal experiments,and the survival rate of TFFAI on HaCaT cells irradiated with UVB rays was investigated.It s antioxidant capacity was determined by ABTS.[Results]The results showed that the highest yield of TFFAI was obtained with 70%ethanol at a solid-to-liquid ratio of 1:50(w/v)and 40℃for 1.5 h by single-factor and orthogonal experiments.Total flavonoids(0.25-1.00 mg/ml)could significantly improve the survival rate of HaCaT cell line.Meanwhile,the maximum absorption peak of TFFAI was found at 283 nm,and in-vitro antioxidant experiment identified that TFFAI had a good clearance rate to ABTS.It suggestes that TFFAI could protect the cells from UVB damage by absorption of UVB rays and anti-oxidation.[Conclusions]TFFAI played a protective role on UVB irradiated cells through UVB ultraviolet absorption and antioxidant pathways.

3D Model Construction and Ecological Environment Investigation on a Regional Scale Using UAV Remote Sensing

The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achieve using satellite remote sensing.Considering the convenient,facilitative,and flexible characteristics of UAV(unmanned air vehicle)remote sensing tech-nology,this study selects a campus as a typical research area and uses the Pegasus D2000 equipped with a D-MSPC2000 multi-spectral camera and a CAM3000 aerial camera to acquire oblique images and multi-spectral data.Using professional software,including Context Capture,ENVI,and ArcGIS,a 3D(three-dimensional)campus model,a digital orthophoto map,and multi-spectral remote sensing map drawing are realized,and the geometric accuracy of typical feature selection is evaluated.Based on a quantitative remote sensing model,the campus ecological environment assessment is performed from the perspectives of vegetation and water body.The results presented in this study could be of great significance to the scientific management and sustainable development of regional natural resources.

Monocular 3D object detection with Pseudo-LiDAR confidence sampling and hierarchical geometric feature extraction in 6G network

The high bandwidth and low latency of 6G network technology enable the successful application of monocular 3D object detection on vehicle platforms.Monocular 3D-object-detection-based Pseudo-LiDAR is a low-cost,lowpower solution compared to LiDAR solutions in the field of autonomous driving.However,this technique has some problems,i.e.,(1)the poor quality of generated Pseudo-LiDAR point clouds resulting from the nonlinear error distribution of monocular depth estimation and(2)the weak representation capability of point cloud features due to the neglected global geometric structure features of point clouds existing in LiDAR-based 3D detection networks.Therefore,we proposed a Pseudo-LiDAR confidence sampling strategy and a hierarchical geometric feature extraction module for monocular 3D object detection.We first designed a point cloud confidence sampling strategy based on a 3D Gaussian distribution to assign small confidence to the points with great error in depth estimation and filter them out according to the confidence.Then,we present a hierarchical geometric feature extraction module by aggregating the local neighborhood features and a dual transformer to capture the global geometric features in the point cloud.Finally,our detection framework is based on Point-Voxel-RCNN(PV-RCNN)with high-quality Pseudo-LiDAR and enriched geometric features as input.From the experimental results,our method achieves satisfactory results in monocular 3D object detection.

Decision Analysis on IoV Routing Transmission and Energy Efficiency Optimization Algorithm with AmBC

The improvement of the quality and efficiency of vehicle wireless network data transmission is always a key concern in the Internet of Vehicles(IoV).Routing transmission solved the limitation of transmission distance to a certain extent.Traditional routing algorithm cannot adapt to complex traffic environment,resulting in low transmission efficiency.In order to improve the transmission success rate and quality of vehicle network routing transmission,make the routing algorithm more suitable for complex traffic environment,and reduce transmission power consumption to improve energy efficiency,a comprehensive optimized routing transmission algorithm is proposed.Based on the routing transmission algorithm,an optimization algorithmbased on road condition,vehicle status and network performance is proposed to improve the success rate of routing transmission in the IoV.Relative distance difference and density are used as decision-making indicators to measure Road Side Unit(RSU)assisted transmission.And the Ambient backscatter communication(AmBC)technology and energy collection are used to reduce the energy consumption of routing relay transmission.An energy collection optimization algorithm is proposed to optimize the energy efficiency of AmBC and improve the energy efficiency of transmission.Simulation results show that the proposed routing optimization algorithm can effectively improve the success rate of packet transmission in vehicular ad hoc networks(VANETs),and theAmBC optimization algorithmcan effectively reduce energy consumption in the transmission process.The proposed optimization algorithm achieves comprehensive optimization of routing transmission performance and energy efficiency.

Li^(+)-ion bound crown ether functionalization enables dual promotion of dynamics and thermodynamics for ambient ammonia synthesis

Electrosynthesis of ammonia from the reduction of nitrogen is still confronted with the limited supply of gas reactant in dynamics as well as high activation barrier in thermodynamics.Unfortunately,despite tremendous efforts devoted to electrocatalysts themselves,they still fail to tackle the above two challenges simultaneously.Herein,we employ a heterogeneous catalyst adlayer-composed of crown ethers associated with Li^(+)ions-to achieve the dual promotion of dynamics and thermodynamics for ambient ammonia synthesis.Dynamically,the bound Li^(+)ions interact with the strong quadrupole moment of nitrogen,and trigger considerable reactant flux toward the catalyst.Thermodynamically,Li^(+)associated with the oxygen of crown ether achieves a higher density of states at the Fermi level for the catalyst,enabling effortless electron transfer from the catalysts to nitrogen and thus greatly reducing the activation barrier.As expected,the proof-of-concept system achieves an ammonia yield rate of 168.5μg h^(-1)mg^(-1)and a Faradaic efficiency of 75.3%at-0.3 V vs.RHE.This system-level approach opens up pathways for tackling the two key challenges that have limited the field of ammonia synthesis.