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Antibacterial properties of lucifensin in Lucilia sericata maggots after septic injury
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作者 Ivana Valachova Emanuel Prochazka +3 位作者 Jana Bohova Petr Novak Peter Takac Juraj Majtan 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第5期358-361,共4页
Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic injury.Mettiods:In our preliminary study we have shown that injuring the maggots with a needle soaked in ... Objective:To investigate the antibacterial properties of lucifensin in maggots of IjicUia sericala after septic injury.Mettiods:In our preliminary study we have shown that injuring the maggots with a needle soaked in lipopolysaccharide solution induced within 24 h lucifensin expression in the fat body and in the grease coupler of the salivary glands.It is assumed that lucifensin is secreted solely from this tissue into the haemoiymph(similar to other insect defensins) and not into secreted/excreted products.We used high-performance liquid chromatography fractionation and radial diffusion assay to investigate the antibacterial properties of haemoiymph extracted from larvae after septic injury.Results:After septic injury,production of lucifensin in the haemoiymph is increased.This led to higher antibacterial activity of such haemoiymph in comparison to non-stimulated larvae.Coclusions:These results suggest that beside the previously demonstrated role of lucifensin in the debridement therapy,lucifensin is simultaneously important as a part of the systematic immune response. 展开更多
关键词 LUCILIA sericata WOUND bacteria DEFENSIN Lucifensin Immune-challenge
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