Coal mining with ploughs is an ideal coal mining technology for thin and thinner coal seams. The existence conditions of coal seams are different for each other, which affects coal mining with ploughs to different deg...Coal mining with ploughs is an ideal coal mining technology for thin and thinner coal seams. The existence conditions of coal seams are different for each other, which affects coal mining with ploughs to different degrees, and the application results are also different. The authors analyze the affecting factors by means of mathematical methods. The research is useful to the wide application of ploughs.展开更多
AIM:To study the morphologic and cellular immunologic changes after homologous transplantation of the abdominal aorta in rats after programmed cryopreservation (-196℃).METHODS:Abdominal aorta was harvested from anest...AIM:To study the morphologic and cellular immunologic changes after homologous transplantation of the abdominal aorta in rats after programmed cryopreservation (-196℃).METHODS:Abdominal aorta was harvested from anesthetized Spraque Dawley (SD) rats for cryopreservation (group B) or immediate implantation (group A). The survival rates and apoptotic rates of aortic endothelial cells (ECs) were examined. The patency rates, histology and cellular immunologic changes of the abdominal aorta were examined on days 1, 3, 7, 14, 30, 60 after transplantation respectively.RESULTS:The survival rate of ECs after programmed cryopreservation was 90.1±1.79%, about 3.4% lower than that of uncryopreservation (93.5±1.96%). The apoptotic rates of ECs was increased after cryopreservation (7.15% vs 4.86%, P<0.05). The patency rate of group B was significantly higher than that of group A (91.6±12.9% vs62.5±26.2%, P<0.01). CD4/CD8 ratio, TCRαβ and CD11b/CD18 ratio of group B were significantly lower than those of group A (P<0.05). Revivification of the cryopreserved abdominal aorta showed normal adventitia and intact smooth muscle cells.CONCLUSION:Cryopreservation can reduce homologous abdominal aortic antigenecity. Even if without administration of immunosuppressive agents, it is still feasible to implement homologous artery grafting in rats.展开更多
文摘Coal mining with ploughs is an ideal coal mining technology for thin and thinner coal seams. The existence conditions of coal seams are different for each other, which affects coal mining with ploughs to different degrees, and the application results are also different. The authors analyze the affecting factors by means of mathematical methods. The research is useful to the wide application of ploughs.
基金Supported by the Natural Science Fundation of Jiangsu Province,China,No.BK2003010the Special Scientific Research Fund of Nanjing,Jiangsu Province,China,No.ZKS0012
文摘AIM:To study the morphologic and cellular immunologic changes after homologous transplantation of the abdominal aorta in rats after programmed cryopreservation (-196℃).METHODS:Abdominal aorta was harvested from anesthetized Spraque Dawley (SD) rats for cryopreservation (group B) or immediate implantation (group A). The survival rates and apoptotic rates of aortic endothelial cells (ECs) were examined. The patency rates, histology and cellular immunologic changes of the abdominal aorta were examined on days 1, 3, 7, 14, 30, 60 after transplantation respectively.RESULTS:The survival rate of ECs after programmed cryopreservation was 90.1±1.79%, about 3.4% lower than that of uncryopreservation (93.5±1.96%). The apoptotic rates of ECs was increased after cryopreservation (7.15% vs 4.86%, P<0.05). The patency rate of group B was significantly higher than that of group A (91.6±12.9% vs62.5±26.2%, P<0.01). CD4/CD8 ratio, TCRαβ and CD11b/CD18 ratio of group B were significantly lower than those of group A (P<0.05). Revivification of the cryopreserved abdominal aorta showed normal adventitia and intact smooth muscle cells.CONCLUSION:Cryopreservation can reduce homologous abdominal aortic antigenecity. Even if without administration of immunosuppressive agents, it is still feasible to implement homologous artery grafting in rats.
