Using the hydrogen-bonding interaction between graphene oxide（GO） and sulfonated polyethersulfone （SPES）, we constructed the multilayer structure of GO and SPES on the polyester tiber mats via layer-by-layer self-...Using the hydrogen-bonding interaction between graphene oxide（GO） and sulfonated polyethersulfone （SPES）, we constructed the multilayer structure of GO and SPES on the polyester tiber mats via layer-by-layer self-assembly. In each self-assembled layer, sulfonic acid groups are arranged along the a^s of fiber, which provides the long-range proton transmission channels, promoting the rapidly proton conduction. The performances of the composite membranes based on SPES and multilayer assembled polyester fiber mats were studied. The results show that the proton conductivity of composite membranes increases with the increasing assembly layers. At the same time, the mechanical properties and methanol-resistance of the composite membranes were obviously improved.展开更多
Lithium ion-doped polyglycerol sebacate scaftbld（PGS）-Li was synthesized by adding lithium ions to po- lyglycerol sebacate（PGS） during its crosslinking process due to the specific effects of lithium ions on period...Lithium ion-doped polyglycerol sebacate scaftbld（PGS）-Li was synthesized by adding lithium ions to po- lyglycerol sebacate（PGS） during its crosslinking process due to the specific effects of lithium ions on periodontal li- gament cells, cementoblasts and the eminent performance of PGS. The molecular mass, composition, structure, porosity, thermal properties, and hydrophilicity of the composite were characterized by gel permeation chromatogra- phy（GPC）, Fourier transform infrared spectroscopy（FTIR）, inductively coupled plasma optical emission spectrome- ter（ICP-OES）, scanning electron microscopy（SEM）, X-ray photoelectron spectroscopy（XPS）, thermogravimetric analyzer（TGA） and contact angle measurments, and the degradation of the material was evaluated by in vitro degra- dation experiments. The biological activity of PGS-Li scaffold was detected by calcein-AM staining and cytotoxicity test. The results indicate that PGS-Li scaffold has been successfully synthesized, which has similar composition and structure to PGS, but slightly larger molecular weight. In addition, the porosity and pore size of PGS-Li scaffold ba- sically meet the requirements of engineering scaffold materials and the seaffold shows better performance in terms of hydrophilicity and thermal stability than PGS. In vitro degradation experimental results show that the degradation rate of PGS-Li scaffold is higher than that of PGS. What＇s more, the results of cytotoxicity test and cell staining show that there is no significant difference in the proliferation and cell morphology of cementoblasts.展开更多
In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protei...In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.展开更多
基金Supported by the National Natural Science Foundation of China（No.21574017）.
文摘Using the hydrogen-bonding interaction between graphene oxide（GO） and sulfonated polyethersulfone （SPES）, we constructed the multilayer structure of GO and SPES on the polyester tiber mats via layer-by-layer self-assembly. In each self-assembled layer, sulfonic acid groups are arranged along the a^s of fiber, which provides the long-range proton transmission channels, promoting the rapidly proton conduction. The performances of the composite membranes based on SPES and multilayer assembled polyester fiber mats were studied. The results show that the proton conductivity of composite membranes increases with the increasing assembly layers. At the same time, the mechanical properties and methanol-resistance of the composite membranes were obviously improved.
文摘Lithium ion-doped polyglycerol sebacate scaftbld（PGS）-Li was synthesized by adding lithium ions to po- lyglycerol sebacate（PGS） during its crosslinking process due to the specific effects of lithium ions on periodontal li- gament cells, cementoblasts and the eminent performance of PGS. The molecular mass, composition, structure, porosity, thermal properties, and hydrophilicity of the composite were characterized by gel permeation chromatogra- phy（GPC）, Fourier transform infrared spectroscopy（FTIR）, inductively coupled plasma optical emission spectrome- ter（ICP-OES）, scanning electron microscopy（SEM）, X-ray photoelectron spectroscopy（XPS）, thermogravimetric analyzer（TGA） and contact angle measurments, and the degradation of the material was evaluated by in vitro degra- dation experiments. The biological activity of PGS-Li scaffold was detected by calcein-AM staining and cytotoxicity test. The results indicate that PGS-Li scaffold has been successfully synthesized, which has similar composition and structure to PGS, but slightly larger molecular weight. In addition, the porosity and pore size of PGS-Li scaffold ba- sically meet the requirements of engineering scaffold materials and the seaffold shows better performance in terms of hydrophilicity and thermal stability than PGS. In vitro degradation experimental results show that the degradation rate of PGS-Li scaffold is higher than that of PGS. What＇s more, the results of cytotoxicity test and cell staining show that there is no significant difference in the proliferation and cell morphology of cementoblasts.
基金Supported by the National Natural Science Foundation of China（Nos.81572082, 81472030, 20973074）, the Science Founda tion of Jilin Provincial Science＆Technology Department, China（No.2011713, 20130413017GH, 20150414015GH）, the Health and Family Planning Commission of Jilin Province, China（No.2015Z041） and the Scientific Support Project from Bethune Department of Jilin University, China（Nos.2012210, 2013207051）.
文摘In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.
