A cloned cDNA designated as MS3 sequence can detect an RNA species with a size of about 6 kb in undifferentiated embryonaI carcinoma (EC) cell lines but not in several differentiated cell lines derived from EC cells.T...A cloned cDNA designated as MS3 sequence can detect an RNA species with a size of about 6 kb in undifferentiated embryonaI carcinoma (EC) cell lines but not in several differentiated cell lines derived from EC cells.This paper deals with the cytoplasmic distribution of this RNA species,MS3 RNA.NO MS3 RNA is observed in the cytoplasm of TDMI(trophoblastoma) cells.In the cytoplasm of PCC3 and F9(EC) cells,however。MS3 RNA is found in both polvsomal fraction and postpolysolnal fraction.About 20~55% MS3 RNA are included in polysome structure.This result implies that MS3 RNA is an actively translated message sequence and the protein specified by MS3 RNA will be a good marker for multipotent early embryonal cells.展开更多
文摘A cloned cDNA designated as MS3 sequence can detect an RNA species with a size of about 6 kb in undifferentiated embryonaI carcinoma (EC) cell lines but not in several differentiated cell lines derived from EC cells.This paper deals with the cytoplasmic distribution of this RNA species,MS3 RNA.NO MS3 RNA is observed in the cytoplasm of TDMI(trophoblastoma) cells.In the cytoplasm of PCC3 and F9(EC) cells,however。MS3 RNA is found in both polvsomal fraction and postpolysolnal fraction.About 20~55% MS3 RNA are included in polysome structure.This result implies that MS3 RNA is an actively translated message sequence and the protein specified by MS3 RNA will be a good marker for multipotent early embryonal cells.
