TO THE EDITOR Angiogenesis consists of the sprouting of capillaries from pre-existing vessels . It is well-known that tumor growth is angiogenesis-dependent. Vascular endothelial growth factor (VEGF) and basic fibrobl...TO THE EDITOR Angiogenesis consists of the sprouting of capillaries from pre-existing vessels . It is well-known that tumor growth is angiogenesis-dependent. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) stimulated vascular endothelial cell proliferation and are involved in the neoplastic angiogenesis of several types of tumors including those of the intestinal tract.Authors usually investigated VEGF and bFGF protein expressions using immunohistochemistry or Western blotting and VEGF and bFGF transcripts using reverse transcriptase polymerase chain reaction (RT-PCR). We recently reported in a previous issue of the World Journal of Gastroenterology that cirrhotic liver tissue levels of these two展开更多
TO THE EDITORWe read with a great interest the recent work of Deli andcolleagues. in the World Journal of Gastroenterology reportingvascular endothelial growth factor (VEGF) expressionin hepatocellular carcinoma (HCC)...TO THE EDITORWe read with a great interest the recent work of Deli andcolleagues. in the World Journal of Gastroenterology reportingvascular endothelial growth factor (VEGF) expressionin hepatocellular carcinoma (HCC) and cirrhotic livertissues. This well-documented work shows that VEGFwas significantly higher in surrounding cirrhotic livertissues than in HCC. Authors assessed VEGF expressionusing immunohistochemistry.The展开更多
AIM: To study the implication of prokineticin 1 (PK1/EG- VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction of PK1/EG-VEGF and PK2/Bv8 was investigated in 10 norma...AIM: To study the implication of prokineticin 1 (PK1/EG- VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction of PK1/EG-VEGF and PK2/Bv8 was investigated in 10 normal, 28 fibrotic and 28 tumoral livers by using real time PCR. Their expression was compared to the expression of VEGF (an angiogenesis marker), vWF (an endothelial cell marker) and to CD68 (a monocyte/macrophage marker). Furthermore, the mRNA levels of PK1/EG-VEGF, PK2/Bv8, prokineticin receptor 1 and 2 were evaluated by real time PCR in isolated liver cell populations. Finally, PK2/Bv8 protein was detected in normal liver paraffin sections and in isolated liver cells by immunohistochemistry and immunocytochemistry. RESULTS: PK2/Bv8 mRNA but not PK1/EG-VEGF was expressed in all types of normal liver samples examined. In the context of liver tumor development, we reported that PK2/Bv8 correlates only with CD68 and showed a significant decrease in expression as the pathology evolves towards cancer. Whereas, VEGF and vWF mRNA were significantly upregulated in both fibrosis and HCC,as expected. In addition, out of all isolated liver cells examined, only Kupffer cells (liver resident macrophages) express significant levels of PK2/Bv8 and its receptors, prokineticin receptor 1 and 2. CONCLUSION: In normal liver PK2/Bv8 and its receptors were specifically expressed by Kupffer cells. PK2/Bv8 expression decreased as the liver evolves towards cancer and did not correlate with HCC angiogenesis.展开更多
基金Supported by La Ligue Nationale Francaise Contre le Cancer (Comitéde la Corrèze et de la Haute Vienne)
文摘TO THE EDITOR Angiogenesis consists of the sprouting of capillaries from pre-existing vessels . It is well-known that tumor growth is angiogenesis-dependent. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) stimulated vascular endothelial cell proliferation and are involved in the neoplastic angiogenesis of several types of tumors including those of the intestinal tract.Authors usually investigated VEGF and bFGF protein expressions using immunohistochemistry or Western blotting and VEGF and bFGF transcripts using reverse transcriptase polymerase chain reaction (RT-PCR). We recently reported in a previous issue of the World Journal of Gastroenterology that cirrhotic liver tissue levels of these two
文摘TO THE EDITORWe read with a great interest the recent work of Deli andcolleagues. in the World Journal of Gastroenterology reportingvascular endothelial growth factor (VEGF) expressionin hepatocellular carcinoma (HCC) and cirrhotic livertissues. This well-documented work shows that VEGFwas significantly higher in surrounding cirrhotic livertissues than in HCC. Authors assessed VEGF expressionusing immunohistochemistry.The
基金INSERM,the Ministère de l'Education Nationale de la Recherche et de la Technologie,the Région Bretagne.No.2079
文摘AIM: To study the implication of prokineticin 1 (PK1/EG- VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction of PK1/EG-VEGF and PK2/Bv8 was investigated in 10 normal, 28 fibrotic and 28 tumoral livers by using real time PCR. Their expression was compared to the expression of VEGF (an angiogenesis marker), vWF (an endothelial cell marker) and to CD68 (a monocyte/macrophage marker). Furthermore, the mRNA levels of PK1/EG-VEGF, PK2/Bv8, prokineticin receptor 1 and 2 were evaluated by real time PCR in isolated liver cell populations. Finally, PK2/Bv8 protein was detected in normal liver paraffin sections and in isolated liver cells by immunohistochemistry and immunocytochemistry. RESULTS: PK2/Bv8 mRNA but not PK1/EG-VEGF was expressed in all types of normal liver samples examined. In the context of liver tumor development, we reported that PK2/Bv8 correlates only with CD68 and showed a significant decrease in expression as the pathology evolves towards cancer. Whereas, VEGF and vWF mRNA were significantly upregulated in both fibrosis and HCC,as expected. In addition, out of all isolated liver cells examined, only Kupffer cells (liver resident macrophages) express significant levels of PK2/Bv8 and its receptors, prokineticin receptor 1 and 2. CONCLUSION: In normal liver PK2/Bv8 and its receptors were specifically expressed by Kupffer cells. PK2/Bv8 expression decreased as the liver evolves towards cancer and did not correlate with HCC angiogenesis.