Objective: To evaluate the ability of Actinomadura madurae(A. madurae) and Nocardia asteroids(N. asteroides), using Candida albicans(C. albicans) as prototypic control, to elicit the activation and IL-1β secretion of...Objective: To evaluate the ability of Actinomadura madurae(A. madurae) and Nocardia asteroids(N. asteroides), using Candida albicans(C. albicans) as prototypic control, to elicit the activation and IL-1β secretion of blood phagocytic cells from healthy donors. Methods: Microcopic evaluation of phagocytosis/activation, cell viability and spectrophotometric quantitation of endocytosis/activation, were assessed by using formazan blue test in human blood phagocytes infected with C. albicans, A. madurae or N. asteroides treated with either normal human serum(NHS) or with decomplemented NHS. Interlukin-1β from culture supernatants of infected polymorphonuclear was tested by ELISA kit assay. Results: Microscopic assay showed that phagocytosis and activation of adherent mononuclear phagocytes were greater with C. albicans followed by A. madurae and then by N. asteroides. Spectrophotometric assay in polymornuclear phagocytes infected with NHS-treated pathogens indicated that activation was similarly higher by C. albicans and A. madurae and lower by N. asteroides. Kinetic assays in infected polymorphonuclear cells showed that viability was decreased by C. albicans and N. asteroides or unaffected with A. madurae. Levels of IL-1β at 8 h of incubation were higher with C. albicans followed by A. madurae whereas lower levels were found with N. asteroides. Conclusions: The extent of cell-viability and activation as well IL-1β secretion may be related with the virulence of C. albicans and N. asteroides and other parameters remain to be explored for assesing the virulence of A. madurae.展开更多
Babesiosis is a rare worldwide-distributed protozoal zoonosis caused by a haemoprotozoan of the genus Babesia,transmitted through bites of tick of the genus Ixodes.The first demonstrated case of human babesiosis in th...Babesiosis is a rare worldwide-distributed protozoal zoonosis caused by a haemoprotozoan of the genus Babesia,transmitted through bites of tick of the genus Ixodes.The first demonstrated case of human babesiosis in theworldwas discovered in Europe,in 1957.However,most of the cases were reported later in the north-east of the United States where Babesia microti has been the cause of over 300 cases of human babesiosis since 1969.In Europe,the most severe cases are observed in asplenic patients infected by a parasite of cattle,the Babesia divergens.Only two cases of babesiosis have been reported in Spain.We present a case of erythema figuratum associated to septic babesiosis in a non-splenectomized man,which is currently the third case of babesiosis in Spain.展开更多
Multinucleate cell angiohistiocytoma (MCAH) was first described by Smith and Wilson- Jones in 1985. It is an uncommon entity but probably underdiagnosed because of lack of recognition by clinicians and pathologists. W...Multinucleate cell angiohistiocytoma (MCAH) was first described by Smith and Wilson- Jones in 1985. It is an uncommon entity but probably underdiagnosed because of lack of recognition by clinicians and pathologists. We report a 47- year- old man with asymptomatic grouped violaceous papules on the dorsum of the hands for 3 years. The histopathological and immunopathological features of ou case revealed characteristics of MCAH similar to the initial description of Smith and Wilson- Jones and other reports.展开更多
文摘Objective: To evaluate the ability of Actinomadura madurae(A. madurae) and Nocardia asteroids(N. asteroides), using Candida albicans(C. albicans) as prototypic control, to elicit the activation and IL-1β secretion of blood phagocytic cells from healthy donors. Methods: Microcopic evaluation of phagocytosis/activation, cell viability and spectrophotometric quantitation of endocytosis/activation, were assessed by using formazan blue test in human blood phagocytes infected with C. albicans, A. madurae or N. asteroides treated with either normal human serum(NHS) or with decomplemented NHS. Interlukin-1β from culture supernatants of infected polymorphonuclear was tested by ELISA kit assay. Results: Microscopic assay showed that phagocytosis and activation of adherent mononuclear phagocytes were greater with C. albicans followed by A. madurae and then by N. asteroides. Spectrophotometric assay in polymornuclear phagocytes infected with NHS-treated pathogens indicated that activation was similarly higher by C. albicans and A. madurae and lower by N. asteroides. Kinetic assays in infected polymorphonuclear cells showed that viability was decreased by C. albicans and N. asteroides or unaffected with A. madurae. Levels of IL-1β at 8 h of incubation were higher with C. albicans followed by A. madurae whereas lower levels were found with N. asteroides. Conclusions: The extent of cell-viability and activation as well IL-1β secretion may be related with the virulence of C. albicans and N. asteroides and other parameters remain to be explored for assesing the virulence of A. madurae.
文摘Babesiosis is a rare worldwide-distributed protozoal zoonosis caused by a haemoprotozoan of the genus Babesia,transmitted through bites of tick of the genus Ixodes.The first demonstrated case of human babesiosis in theworldwas discovered in Europe,in 1957.However,most of the cases were reported later in the north-east of the United States where Babesia microti has been the cause of over 300 cases of human babesiosis since 1969.In Europe,the most severe cases are observed in asplenic patients infected by a parasite of cattle,the Babesia divergens.Only two cases of babesiosis have been reported in Spain.We present a case of erythema figuratum associated to septic babesiosis in a non-splenectomized man,which is currently the third case of babesiosis in Spain.
文摘Multinucleate cell angiohistiocytoma (MCAH) was first described by Smith and Wilson- Jones in 1985. It is an uncommon entity but probably underdiagnosed because of lack of recognition by clinicians and pathologists. We report a 47- year- old man with asymptomatic grouped violaceous papules on the dorsum of the hands for 3 years. The histopathological and immunopathological features of ou case revealed characteristics of MCAH similar to the initial description of Smith and Wilson- Jones and other reports.
