CLINICAL OBSERVATION AND TREATMENT OF HYPERKINESIA IN CHILDREN BY TRADITIONAL CHINESE MEDICINE

Sixty-six children with hyperkinesia were treated with the Yizhi (wit-increasing) syrup, af-ter which their scores on behavior dropped, their school records improved, and the rate of appearance of soft neurotic signs lowered, all three changes being significant, giving a total effectiveness rate of 84.8%. After the treatment, examination of the 24-hour urine showed significant increases in its content of norepinephrine (NE), dopamine (DA), 3-4 dihydroxy phenylacetic acid (DOPAC), cyclic adenosine monophosphate (cAMP), and creatinine (Cr).

Treatment of nutcracker syndrome with spermatic vein ligation and iliac vein anastomosis:Case report of three cases

Three patients with nutcracker syndrome(NCS) were enrolled in the study.Their clinical symptoms mainly included proteinuria and serious varicocele.Color Doppler ultrasound and computed tomography showed that the patients had constricted abdominal aortas,reduced superior mesenteric artery angles,and thinned left renal veins.Patients then underwent left spermatic vein ligation and iliac vein anastomosis.They were checked tliree months after their operation,and results showed that their sperms were improved and their proteinuria disappeared. The color Doppler ultrasound showed new cycle pathways.NCS clinical manifestations are complicated,and need combination diagnosis of symptoms and auxiliary examination to detennine surgical indications.Ligation of spermatic vein and vein anastomosis can be used to treat NCS.

Experimental Study on Gufusheng in Treatment of Steroid-Induced Ischemic Necrosis of Femoral Head in Rabbits

Objective: To explore the mechanisim of compound Gufusheng in treatment of steroid-induced ischemic necrosis of femoral head (SINFH). Thirty-two rabbits were randomly divided into a blank control group (n=12) and a model group (n=20). The rabbits of the model group were established by intramuscular injection of prednisone acetate (0.32mg/kg/d) for 8 weeks, and 2 rabbits in each group were killed at the 6th and the 8th week respectively to investigate whether the model was successfully prepared. Then remainder in the model group was again randomly divided into group A (Gufusheng group, n=8) and group B (model group, n=8), while the rest of rabbits in the blank group constituted group C (n=8). The rabbits in group B and C were fed with normal saline. After treatment for 4 weeks, the serum insulin-like growth factor-1 (IGF-1) and tumor necrosis factor-α (TNF-α) levels in the rabbits of the 3 groups were determined by radioimmunoassay (RIA). The serum TNF-α decreased significantly and the IGF-1 level increased significantly in the Gufusheng group as compared with the model group (both P＜0.01). Compound Gufusheng can prevent SINFH and promote repair of the tissue of necrosis of femoral head possibly via decreasing the TNF-α and increasing IGF-1 level.

Effects of Bushen Huoxue Decoction on Nitric Oxide (NO) in Serum,Articular Cartilage and Synovium in Rabbits of Knee Osteoarthritis

Forty-eight New Zealand rabbits were divided into normal group (n=18), control group (n=18) and Chinese herbs treatment group (n=12) randomly. The rabbits in the normal group received sham-operation, and the OA model was established by Hulth's method. All the rabbits in the treatment group were given Bushen Huoxue Decoction (补肾活血汤) from the 6th week after the operation. At 6th, 8th and 12th week after the operation, the NO concentrations of the serum, joint cartilage and synovium were examined. Results: Indicated that the NO concentrations of the serum,joint cartilage and synovium in the control group were all significantly higher than those in the normal group, with the joint cartilage more obvious (P<0.05). In the Chinese herbs treatment group the NO concentrations in all the parts obviously decreased as compared with the control group (P<0.05). It is suggested that Bushen Huoxue Decoction decrease the levels of NO in the serum, synovium and joint cartilage in the OA rabbit.

Olfactory ensheathing cell transplantation and inhibition of NogoA,NgR and RhoA expression in the damaged zone to ameliorate spinal cord injury

BACKGROUND： Olfactory ensheathing cell （OEC） transplantation promotes repair of spinal cord injury. Neural regeneration inhibits binding of the myelin protein Nogo to its receptor （NgR）, activates downstream inhibitory signal RhoA, and leads to axonal degeneration. OBJECTIVE： To determine the relationship between OECs transplantation for spinal cord injury and NogoA, NgR, and RhoA protein expression in the damaged zone. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed from September 2006 to May 2007 at the Key Laboratory of Environment and Genes in Xi＇an Jiaotong University School of Medicine, China. MATERIALS： OECs were harvested from healthy, adult, male, Sprague Dawley rats aged 6 months. Mouse anti-rat NogoA, NgR, and RheA monoclonal antibodies were utilized for detection. METHODS： A total of 40 adult Sprague Dawley rats were randomly assigned to four groups： normal, model, OECs, and DF12, with 10 animals in each group. Transverse section spinal cord injury was established in the OECs and DF12 groups, followed by injection of 1μL OECs suspension （1×10^8/mL） or equivalent DF12 medium at 1 mm above and below the injury site. MAIN OUTCOME MEASURES： Immunohistochemistry and Western blot were utilized to detect NogoA, NgR, and RhoA expression in the spinal cord injury lesions. Morphological changes were observed by argyrophilia staining, and lower extremity function of the animals was assessed using Basso, Beattie, and Bresnahan scores. RESULTS： Eight weeks following OECs transplantation, a significant increase in new axons was observed in the OECs group, and nerve fibers crossed the injury site to repair spinal cord injury. Qualitative and quantitative results from the OECs group were superior to the model and DF12 groups. At 8 weeks after transplantation, Basso, Beattie, and Bresnahan scores were significantly greater in the OECs group compared with the model and DF12 groups （P 〈 0.01）, but expression of NogoA, NgR, and RhoA protein was significantly decreased compared with the model and DF12 groups （P〈 0.05）. CONCLUSION： OEC transplantation could inhibit NogoA, NgR, and RhoA expression in spinal cord injury lesions, thereby promoting repair of spinal cord injury.

A Clinical Study of Suogudan Granule (索骨丹颗粒) in the Treat ment of Rheumatoid Arthritis

Objective： To study the clinical efficacy of Suogudan Granule （SGDG, 索骨丹颗粒） in the treatment of rheumatoid arthritis （RA）. Methods： Ninety patients with RA were randomly divided into the treated group and the control group. The treated group was administered orally with SGDG 6 g each time, thrice a day, while the control group with the combined therapy of Fenbid Oapsules 0.3 g each time, twice a day and Tripterygium tablet 20 mg each time, thrice a day. The treatment course for both groups was 6 weeks. The changes of clinical symptoms and signs, and laboratory indices such as erythrocyte sedimentation rate （ESR）, rheumatoid factor （RF）, antistreptolysin O （ASO）, routine examination of blood and urine, liver and kidney function, etc. before and after treatment were observed. Results： （1） The total effective rate in the treated group （88.0 %） was obviously higher than that in the control group （67.5 %） with significant difference （P〈0.05）. （2） The improvement in arthralgia, joint swelling, time of morning stiffness, 15-meter walking, analgesia initiation and persistence in the treated group was better than that in the control group （P〈0.05, P〈0.01）, but there was no obvious difference in improvement of joint tenderness, range of joint motion, grip strength, and initiating detumescence time （P〉0.05）. （3） The improvement in ESR and RF in the treated group was better than that in the control group with significant difference （P〈0.05）. The negative-conversion rate of ASO in the treated group was also higher than that in the control group （P〈0.01）. （4） No evident abnormality in blood, urine, liver or kidney function was found in either group. Conclusion： SGDG is effective and safe for the treatment of RA.

The Role of Different Therapeutic Courses in Treating 47 Cases of Rheumatoid Arthritis with Acupuncture

Objective: To observe the effect of acupuncture therapeutic courses on rheumatoid arthritis. Method: Forty-seven patients were treated with acupuncture for 6 courses and at the end of the third and sixth course of treatment, the therapeutic effects of acupuncture on morning rigidity, swelling and pain of joints as well as rheumatoid factor (RF), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were observed. Result: At the end of the third course, the markedly effective rate was 34.0% for morning rigidity, 9.5% for swelling and 23.4% for pain, the RF negative-turning rate was 2.2%, and there was no significant difference (P>0.05) in the reduction of ESR and CRP as compared to that before treatment. However, at the end of the sixth course, the markedly effective rate was 80.9% for morning rigidity, 64.3% for swelling and 87.2% for pain, the RF negative-turning rate was 26.7%, and there was a very significant difference (P<0.01) in the reduction of ESR and CRP as compared to that before treatment. Conclusion: More acupuncture therapeutic courses can bring about better therapeutic results on rheumatoid arthritis.

CLINICAL OBSERVATION ON EFFICACY OF ELECTRO-ACUPUNCTURE THERAPY IN HYPERPLASIA OF MAMMARY GLANDS AND ITS EFFECT ON IMMUNOLOGICAL FUNCTION

Based on our work on electro-acupuncture therapy of hyperplasia of mammary glands and in order to observe further the relationship between immunological function and E2 in hyperplasia of mammary glands, we determined the change not only in immunological function before and after electro-acupuncture in 44 in-patients with hyperplasia of mammary glands and having the same menstruation date, but also in electro-acupuncture treated rat hyperplasia of mammary glands model. We found that there was decrease of immunological function in hyperplasia of mammary glands and this function could be remarkably enhanced by electro-acupuncture both in hyperplasia of mammary glands patients and in rat hyperplasia of mammary glands model. The mechanism implies it might be that the inhibitory effect on immunological function exerted by high concentration of E2 was lowered by electro-acupuncture, hence the immunological function was reinforced. The results of this study are important in exploration of both the pathogenesis of this disease and the mechanism of electro-acupuncture, and in reducing the incidence of breast carcinoma.

TREATMENT OF CHRONIC CORONARY INSUFFICIENCY WITH ACUPUNCUTRE ON XIMEN POINT

For many years in treatment of acutemyocardial insufficiency,the authors havefound that acupuncture on Ximen(P 4)im-proves myocardial blood supply and relievesthe clinical symptoms.For further study ofthe therapeutic effect of Ximen(P 4),the au-thors have treated a series of coronaryinsufficiency patients by acupuncture on thispoint with concurrent observation on thechanges in EKG.The results are reported inthe following.

Study on Endoscopic Treatment of Non-Varicose Upper Gastrointestinal Bleeding

Upper gastrointestinal bleeding is a common clinical disease with a relatively high incidence rate,and it is also an acute bleeding disease with a greater impact.If not rescued in time,it will greatly threaten the patient's physical and mental health and may even be fatal,especially in cases of non-varicose upper hemorrhage,which is closely related to digestive ulcers.In most cases,related diseases are selflimiting-However,some of the cases are relatively urgent and require internal meridian treatment to promote coagulation and improve existing disease situation.For endoscopic treatment process,related treatment methods have certain specificities,and their characteristics need to be fully understood.

Potential Value of Splenectomy for the Life Quality and Long-term Survival of Patients with Cirrhosis

Patients with cirrhosis will gradually develop portal hypertension(PHT)and esophagogastric varices,and subsequently the more severe and often lethal complications such as bleeding esophageal varices,ascites,spontaneous bacterial peritonitis,renal dysfunction and hepatic encephalopathy.To date,there is still not any effective therapy for the prevention of the development these serious complications.The initial factor leading to PHT is an increase in hepatic resistance.Then,the increase in portal blood flow contributes to the maintenance and exacerbation of PHT,as well as the formation of esophagogastric varices and lethal complications.Increasing evidence shows that,enlarged spleen not only contributes to the increase in portal venous inflow,but also plays a contributory role in promoting almost all lethal complications of cirrhosis through its resulted hypersplenism together with other mechanisms.So,we hypothesize that prophylactic splenectomy may efficiently delay the progression of cirrhosis and improve life quality and long-term survival of patients with cirrhosis.

Effects of Preventive Administration of Juanbi Capsules on TNF-α、 IL-1 And IL-6 Contents of Joint Fluid in the Rabbit with Knee Osteoarthritis

Objective:To probe into the mechanism of the Chinese herbs with functions of reinforcing kidney and supplementing qi for preventing knee osteoarthritis of the rabbit. Methods: Totally 72 healthy Japan long-ear white rabbits, aged 4 months, were randomly divided into 6 groups, blank group (A), model group (B), high dose Chinese herb group (C), middle dose Chinese herb group (D), small dose Chinese herb group (E), aminoglucose hydrochloride capsule control group (F), 12 rabbits in each group. All the rabbits in the groups, except the group A, were fixed with plaster cast for six weeks to establish rabbit knee osteoarthritis. At the same time of modeling, the different doses of Juanbi Capsules and aminoglucose hydrochloride capsule were administrated intragastrically in the group C, D, E, F, respectively, for 4 weeks, for preventive treatment. In the group B, the rabbit was administrated intragastrically with equal volume of normal saline to the medication groups, twice each day, in the morning and the evening, and in the group A, nothing was administrated. After modeling for 6 weeks, the joint fluid was taken and TNF-α, IL-1 and IL-6 contents were detected with ELISA method, and the articular cartilage was taken for macroscopic and microscopic examinations. Results: In all the preventive treatment groups, the articular cartilage color changed to varying degrees with formation of osteophyte and bone cyst, superficial erosion on the chondral articular surface, and the cartilage defect reached to the mid layer in a part of specimens with cartilage exfoliation, but which in the extent were significantly lower than those in the model group. There were significant differences between the group A and B in TNF-α , IL-1 and IL-6 contents in the joint fluid (P<0.05), indicating that the modeling is successful; and there were significant differences as group B compared with the group C,D, E, F, showing that TNF-α , IL-1 and IL-6 contents are decreased in all the medication groups; and significant differences between group C, D, E suggests that the increase of Chinese herb doses strengthened the effect of reducing TNF-α , IL-1 and IL-6 contents in joint fluid. Conclusion: The Juanbi Capsule prevents osteoarthritis possibly through decreasing serum TNF-α, IL-1 and IL-6 contents.

Clinical Effects of Acupuncture for Diabetic Peripheral Neuropathy

Objective:To observe the clinical effects of acupuncture for diabetic peripheral neuropathy.Methods:Totally 65 patients were randomly divided into a treatment group of 32 cases and a control group of 33 cases. On the basis of conventional treatment of diabetes,acupuncture was used in the treatment group,and inositol was orally administered in the control group.During a 3-month treatment,changes in the symptoms were observed.Results:In the treatment group,16 cases were markedly relieved,12 cases improved,and 4 cases failed,with a total effective rate of 87.5%.In the control group,7 cases were markedly relieved,14 cases improved and 12 cases failed,with a total effective rate of 63.6%.There was a significant difference in the total effective rate between the 2 groups(P<0.05) .Conclusion:Acupuncture may show good effects for diabetic peripheral neuropathy.

Clinical Study on Treatment of Vascular Dementia with Scalp Acupuncture

Objective： To explore a treatment method for vascular dementia. Methods： Sixty patients were randomly allocated into two groups. The observation group was treated with long-term needle-retaining method of scalp acupuncture and the control group was treated by medications. Evaluation was made respectively before and after the treatment by means of Hasegawa dementia scale （HDS）, neurological function deficit （NFD） score and main symptom （MS） score. Results： There were statistically significant differences in HDS, NFD score and MS score in both groups （P〈0.01） before and after the treatment. There were statistically significant differences in HDS, NFD score and MS score between the two groups （P〈0.05） before and after the treatment. The total effective rate was 86.8% in the observation group and 80.0% in the control group. There was a statistically significant difference in the effects between the two groups （P〈0.05）. Conclusion： This long-term needle-retaining method of scalp acupuncture is precisely effective for vascular dementia.

Study on Perfume Stimulating Olfaction with Volatile Oil of Acorus Gramineus for Treatment of the Alzheimer’s Disease Rat

Objective:To probe into the therapeutic efect of perfume stimulating olfaction with volatile oil of Acorus Gramineus on the Alzheimer’s disease (AD) rat. Methods: Totally 50 adult SD rats, male,weighing 300±10 g, were randomly divided into 5 groups, normal group (group A), olfactory nerve severing model group (group B), AD model group (group C), AD model plus perfume stimulation group (group D), AD model olfactory nerve severing plus perfume stimulation group (group E), 10 rats in each group. After perfume stimulation, Morris maze test was conducted for valuating the learning and memory ability; Malondaldehyde (MDA) content, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the brain, and the brain weight were detected. Results: Compared with the AD model group,the average escape latency and swimming distance in 6 days were significantly shorter than those in the group A, B, D (P<0.01), with no significant differences between the group C and the group E (P>0.05); Compared with the group A, B and D, MDA content in the group C significantly increased (P<0.01), and SOD and GSH-Px activities significantly decreased (P<0.01), and brain weight/body weight decreased significantly in the group C (P<0.01), with no significant differences between the group C and the group E (P>0.05). Conclusion: Perfume stimultating olfaction with volatile oil of Acorus Gramineus can significantly increase the learning-memory ability, decrease MDA content and increase SOD and GSH-Px activities and weight of brain in AD rats.

Post-marketing Re-evaluation of Tongxiening Granules (痛泻宁颗粒) in Treatment of Diarrhea-Predominant Irritable Bowel Syndrome:A Multi-center,Randomized,Double-Blind,Double-Dummy and Positive Control Trial

Objective: To evaluate the efficacy and safety of Tongxiening Granules(痛泻宁颗粒, TXNG) in the treatment of irritable bowel syndrome with predominant diarrhea(IBS-D). Methods: A randomized, double-blind, double-dummy, and positive paral el control ed clinical trial was conducted from October 2014 to March 2016. Total y 342 patients from 13 clinical centers were enrolled and randomly assigned(at the ratio of 1:1) to a treatment group(171 cases) and a control group(171 cases) by a random coding table. The patients in the treatment group were administered oral y with TXNG(5 g per time) combined with pinaverium bromide Tablet simulator(50 mg per time), 3 times per day. The patients in the control group were given TXNG simulator(5 g per time) combined with pinaverium bromide Tablets(50 mg per time), 3 times per day. The treatment course lasted for 6 weeks. The improvement of Irritable Bowel Syndrome Symptom Severity Score(IBS-SSS) was used to evaluate the primary outcome. Secondary outcomes included adequate relief(AR) rate, Irritable Bowel Syndrome-Quality of Life Questionnaire(IBS-QOL), Hamilton Anxiety Scale(HAMA), Hamilton Depression Scale(HAMD), and the recurrence rate at fol ow-ups. Safety indices including the adverse events(AEs) and related laboratory tests were evaluated. Results: Primary outcome: IBS-SSS at baseline, weeks 2, 4, 6 showed no statistical significance in both full analysis set(FAS) and per protocol set(PPS, P>0.05). After 6 weeks of treatment, the total effective rate of IBS-SSS scores in the treatment group(147/171, 86.0%) was higher than the control group(143/171, 83.6%) by FAS(P>0.05). In regard to secondary outcomes, after 6-week treatment, there was no significant difference in AR rate, total score of IBS-QOL, improvement of HAMD and HAMA total scores between the two groups(P>0.05). The recurrence rate at 8-week fol ow-up was 12.35%(10/18) in treatment group and 15.79%(12/76) in control group, respectivery(P>0.05). A total of 21 AEs occurred in 15 cases, of which 11 occurred in 8 cases in the treatment group and 10 AEs in 7 cases in the control group. The incidence of AEs had no statistical significance between the two goups(P>0.05). Conclusion: Tongxiening Granules could relieve the symptoms of patients with IBS-D and the treatment effect was comparable to pinaverium bromide.(No. ChiCTR-IPR-15006415)

Clinical effect of Sidong Wubu method in treatment of closed fracture of upper limbs

OBJECTIVE:To objectively evaluate the clinically curative effect of sidong wubu method on closed fracture of upper limbs.METHODS:A single-blind randomized controlled trial was used to divide 654 patients with closed fracture of upper limbs at early stage into two groups.298 patients in the surgical group were treated with open reduction and internal fixation and 356 patients in the treatment group with sidong wubu method for 6 months as a course of treatment.RESULTS:As for short-term curative effect(after 6 courses of treatment),the total effective rate was 97.7% in the treatment group and 92.9% in the control group,and the excellent and good rate was 83.7% and 76.5% respectively.Fracture-healing time,treatment cost,function-recovering time,scores of symptoms and signs obviously declined in both groups with remarkable difference between the two groups.As to long-term curative effect(after follow-up visit for one year to 5 years and 2 months),there was still noticeable difference(χ 2 = 7.536,P<0.05) in total curative effect and in excellent and good rate between the two groups.CONCLUSION:With low cost,short treatment course,good function and other advantages,sidong wubu method can be first used to treat closed fracture of upper limbs.

Treatment of 35 Cases of Epistaxis with Acupressure on Jingbailao （Ex-HN 15）

Nasal bleeding, namely epistaxis, is a common symptom in clinical practice, which is often caused by nasal, or systemic diseases. The author used acupressure on Jingbailao （Ex-HN 15） in treating 35 cases of epistaxis. Now it is reported as follows.

Clinical Experience of Acupoints Needling Sequences

This paper summarizes and analyzes the needling sequences in clinical practice. It is concluded that between the cardinal acupoints and adjunct acupoints, the former ones are first needled; in the emergency conditions, the branch （biao） acupoints are first needled; in the chronic diseases, the root （ben） are first needled; as to several diseases, the first-happened disease is first treated; furthermore, the needling sequence also depends upon the treatment method, and the acupoints of special functions are first needled.

A simple,water-soluble,Fe^(3+)-selective fluorescent probe and its application in bioimaging

A simple, water-soluble, Fe3＋-selective fluorescent probe, derived from rhodamine B, was synthesized and characterized. The probe exhibits a fluorescence response toward Fe3＋ with acceptable sensitivity and selectivity and even facilitates visual or naked-eye detection of Fe3＋. The experiment results show that the response of the probe to Fe3＋ is pH-independent over a wide range of 4.0-10.0. In addition, fluorescence microscopic imaging experiments have proven that the probe is cell permeable and can be used for monitoring intracellular Fe3＋ in living cells.