Effects of temperature on self-interaction of human-like collagen （HLC） were investigated by hydrophobic in- teraction chromatography, calorimetric measurement, and sodium dodecyl sulphate-polyacrylamide gel electro...Effects of temperature on self-interaction of human-like collagen （HLC） were investigated by hydrophobic in- teraction chromatography, calorimetric measurement, and sodium dodecyl sulphate-polyacrylamide gel electropho- resis （SDS-PAGE） analysis. Results show that three types of interaction roles may exist between ttLC molecules at 3--50 ℃, which were divided into three narrower temperature ranges. In temperature range from 3--22 ℃, hy- drogen bonding plays a key role in the formation of a gelatinous aggregate. In the range of 22--38 ℃, hydrophobic bonds accompanied by hydrogen bonds are involved in the formation compact aggregates. When temperature is above 38 ℃ the hydrophobic effect formed in the HLC monomer results in the loss of its ability to self-interact.展开更多
文摘Effects of temperature on self-interaction of human-like collagen （HLC） were investigated by hydrophobic in- teraction chromatography, calorimetric measurement, and sodium dodecyl sulphate-polyacrylamide gel electropho- resis （SDS-PAGE） analysis. Results show that three types of interaction roles may exist between ttLC molecules at 3--50 ℃, which were divided into three narrower temperature ranges. In temperature range from 3--22 ℃, hy- drogen bonding plays a key role in the formation of a gelatinous aggregate. In the range of 22--38 ℃, hydrophobic bonds accompanied by hydrogen bonds are involved in the formation compact aggregates. When temperature is above 38 ℃ the hydrophobic effect formed in the HLC monomer results in the loss of its ability to self-interact.
