This paper aims to research the effects of combined application of humic acid and urea on the wheat yield and yield component,and establish rational fertilizer application scheme of wheat. Split plot experiment was ad...This paper aims to research the effects of combined application of humic acid and urea on the wheat yield and yield component,and establish rational fertilizer application scheme of wheat. Split plot experiment was adopted. Two types of phosphorus fertilizer( adding humic acid or not) was assigned to the main plot,and four types of top dressing modes( different amount of urea and humic acid urea) were used in subplots. Effects of combined application of humic acid and urea on the wheat yield and yield component were researched. 375 kg / ha ordinary DAP + 150 kg / ha urea was used as base fertilizer; topdressing of 300 kg / ha urea humate could effectively promote the plant height,leaf area coefficient and aboveground dry matter amount. Adding humic acid in urea in topdressing significantly enhanced the wheat yield and economic coefficient of wheat. Adding humic acid in urea in topdressing has better effects of yield increasing than base fertilizer,and can be used for large scale extension.展开更多
Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus...Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials, to compare the effects of pH in suspension medium, addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.展开更多
基金Supported by National Modern Agricultural Industrial Technological System Foundation of the Ministry of Agriculture(CARS-3)
文摘This paper aims to research the effects of combined application of humic acid and urea on the wheat yield and yield component,and establish rational fertilizer application scheme of wheat. Split plot experiment was adopted. Two types of phosphorus fertilizer( adding humic acid or not) was assigned to the main plot,and four types of top dressing modes( different amount of urea and humic acid urea) were used in subplots. Effects of combined application of humic acid and urea on the wheat yield and yield component were researched. 375 kg / ha ordinary DAP + 150 kg / ha urea was used as base fertilizer; topdressing of 300 kg / ha urea humate could effectively promote the plant height,leaf area coefficient and aboveground dry matter amount. Adding humic acid in urea in topdressing significantly enhanced the wheat yield and economic coefficient of wheat. Adding humic acid in urea in topdressing has better effects of yield increasing than base fertilizer,and can be used for large scale extension.
基金Supported by National Natural Science Foundation of China(31201218)National Science and Technology Project of "Twelfth Five Year" Plan of China(2011BAD35B01)+3 种基金Taishan Scholars Program of Shandong Province(Crop Cultivation and Breeding)Basic Research Program of Science and Technology Project of Qingdao City[12-1-4-5-(11)-jch]Starting Fund for High-level Personnel of Qingdao Agricultural University(No.630740)Shandong Key Laboratory of Maize Breeding and Cultivation Techniques
文摘Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials, to compare the effects of pH in suspension medium, addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.
