Effects of special brain area regional cerebral blood flow abnormal perfusion on learning and memory function and its molecular mechanism in rats

To study the effect of special brain area regional cerebral blood flow(rCBF)abnormal perfusion on learning and memory function and its molecular mechanism,64 adult male healthy Sprague-Dawley(SD)rats were randomly divided into two groups,the false operation group(control group)and the operation group(model group).After surgical operation,the operation group undertook bilateral common carotid artery permanent ligation,while the other group did not.Learning and memory function were measured by Y-maze at 4 h,8 h,24 h and 3 d after surgical operation,respectively.The rCBF of the right frontal lobe and hippocampus was also detected by the PerifluxPF model laser Doppler flowmetry,and the expressions of c-fos or c-jun or Bcl-2 and Bax were also measured by immune histochemistry S-P method accordingly.Results showed that the rCBF of the right frontal lobe and hippocampus in the operation group was significantly lower than that in the false operation group(P,0.05).The learning indexes,error number(EN),day of reach standard and total reaction time(TRT)in the operation group,were significantly higher than that in the false operation group(P,0.05).However,the initiative evasion rate in the operation group was significantly lower than that in the false operation group.The study also found that the rCBF was relatively more,the indexes(EN,the day of reach standard and TRT)relatively fewer,but the initiative evasion rate and the memory keeping rate were relatively more.The positive expression and the average absorbency of Fos and Jun in the operation group were significantly higher than that in the false operation group(P<0.05).Furthermore,Bax and Bcl-2 positive cells were all increased over time in the operation group,and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group(P<0.01).In conclusion,rCBF decrease can impair the learning and memory function in rats,which may be related to the increase of the expression ratio of c-fos or c-jun or Bcl-2 or Bax in the frontal cortex and hippocampus.

Effects of regional cerebral blood flow perfusion on learning and memory function and its molecular mechanism in rats

Objective:To study the effects of regional cerebral blood flow(r CBF)perfusion on learning and memory function in special brain areas and its molecular mechanism in rat.Methods:Sixty-four adult male healthy Sprague-Dawley(SD)rats were randomly divided into two groups:A false operation group and an operation group.The false operation group was randomly divided into four subgroups(A_0,B_0,C_0,and D_0)and the operation group was randomly divided into four subgroups(A,B,C,and D),with eight rats in each subgroup.The operation group underwent bilateral common carotid artery permanent ligation,while the other group only underwent a skin incision without the bilateral common carotid artery permanent ligation.Learning memory function of rats in each subgroup was measured using a Y-maze at 4 h,8 h,24 h,and 3 days after surgery.The r CBF in the right frontal lobe and hippocampus was detected using the Periflux PF model laser Doppler flowmetry and c-fos,c-jun,Bcl-2,and Bax protein expression in the right frontal lobe and hippocampus was measured using immunohistochemistry.Results:The r CBF in the right frontal lobule division and right hippocampus division was significantly lower in the operation group than in the false operation group(P<0.05).The error number(EN),time to reach the target,and total reaction time(TRT)for the learning index using the Y-type labyrinth test in the operation group were significantly higher than that in the false operation group(P<0.05);however,the active avoid rate in the operation group was significantly lower than that of the false operation group.Expression of c-fos and c-jun as well as the average absorbency in the right frontal lobule division and right hippocampus division in the operation group were significantly higher than those in the false operation group(P<0.05).The number of Bax and Bcl-2-positive cells was significantly higher in the operation group,and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group(P<0.01).Conclusions:r CBF decrease can impair learning and memory function in rats,which may be related to the increased expression of c-fos,c-jun,Bcl-2,and Bax proteins in the frontal cortex and hippocampus.