Background Soil-transmitted helminths(STHs)were previously endemic in Shandong Province,China.This study aimed to analyze the STHs prevalence trend and the natural,social,and human cognitive and behavioural factors in...Background Soil-transmitted helminths(STHs)were previously endemic in Shandong Province,China.This study aimed to analyze the STHs prevalence trend and the natural,social,and human cognitive and behavioural factors infuencing the discrepancies between high and low infection levels from 2016 to 2020 in Shandong Province in eastern China.Methods STHs surveillance data of Shandong Province from 2016 to 2020 were obtained from China Information Management System for Prevention and Control of Parasitic Diseases.STHs infections were detected by modifed Kato–Katz method.Comprehensive information on the natural and social factors,STHs-related knowledge and behaviours were collected through questionnaire surveys.Retrospective spatial scan analysis was performed using SaTScan v10.1 to evaluate any identifed spatial clusters of STHs infection for statistical signifcance and Bayes discriminant analysis was used to discriminate the high or low infection groups of the villages.Results In total,72,160 participants were involved in our survey from 2016 to 2020.The overall STHs prevalence rate was 1.13%,with the eastern region of Shandong Province having the highest rate(2.02%).The predominant species was T.trichiura,with the prevalence rate of 0.99%and the≥70-year age group possessed the highest rate of 2.21%.The STHs prevalence rate showed an annual linear downward trend from 2016 to 2020(χ_(trend)^(2) =127.600,P<0.001).Respondents aged≥60 years had the lowest awareness level of STHs-related prevention knowledge(all P<0.05),and were the most likely to adopt the practice of fertilizing with fresh stool(χ^(2)=28.354,P<0.001).Furthermore,the southern region demonstrated the highest temperature and rainfall level and the lowest GNP and annual net income per capita(all P<0.05).Conclusions There is a remarkable declining in STHs prevalence in Shandong Province from 2016 to 2020.However,the prevalence rates of STHs especially T.trichiura in the southern and eastern regions were still high,and the elderly were more susceptible to be infected with STHs owning to their low awareness level of STHS-related prevention knowledge and high adoption rate of dangerous production and living behaviours.Integrated approaches of health education,environment improvement and behaviour change should be strengthened to obtain a further reduction of STHs prevalence in China.展开更多
Objective: To investigate the azole susceptibility of Candida albicans(C.albicans)from vulvovaginal candidosis patients and to analyze the relationship between ERG11 gene mutations in these isolates and azole resistan...Objective: To investigate the azole susceptibility of Candida albicans(C.albicans)from vulvovaginal candidosis patients and to analyze the relationship between ERG11 gene mutations in these isolates and azole resistance.Methods: Three hundred and two clinical isolates of Candida species were collected.Azole susceptibility was tested in vitro in microdilution studies. The ERG11 genes of 17 isolates of C. albicans(2 susceptibles, 5 dose-dependent resistants and 10 resistants) were amplified and sequenced.Results: Of the 302 isolates collected, 70.2% were C. albicans, of which 8.5%, 3.8% and4.2% were resistant to fluconazole, itraconazole and voriconazole, respectively. In total,27 missense mutations were detected in ERG11 genes from resistant/susceptible dosedependent isolates. Among them, Y132 H, A114 S, and Y257 H substitutions were most prevalent and were known to cause fluconazole resistance. G464 S and F72 S also have been proved to cause fluconazole resistance. Two novel substitutions(T285A, S457P) in hotspot regions were identified.Conclusions: Twenty seven mutations in the ERG11 gene were identified in azoleresistant C. albicans isolates, which indicated a possible relation with the increase in resistance to azole drugs and the recurrence of vulvovaginal candidosis. The relationship of two novel substitutions(T285A, S457P) with fluconazole resistance needs to be further verified by site-directed mutagenesis.展开更多
China used to be one of the most heavily endemic countries for lymphatic filariasis(LF)in the world.There were 864 endemic counties/cities in 16 provinces/autonomous regions/municipalities(P/A/M)with a total populatio...China used to be one of the most heavily endemic countries for lymphatic filariasis(LF)in the world.There were 864 endemic counties/cities in 16 provinces/autonomous regions/municipalities(P/A/M)with a total population of 330 million at risk of infection.Since the founding of the People’s Republic of China in 1949,the Chinese Government has designated the control of the disease to be a top priority.Due to decades of sustained efforts,close cooperation related to LF control among government departments,and active participation of endemic populations,an all-round campaign for prevention and control has been carried out vigorously and successfully.Over many years,great achievements have been made through persistent endeavors of Chinese scientists and disease control workers.The ultimate goal to eliminate LF in the country was achieved in 2006.展开更多
Background Neurocysticercosis is the infection of the nervous system by the larvae of Taenia solium （T. solium）. Despite continuous effort, the experimental diagnosis of neurocysticercosis remains unresolved. Since ...Background Neurocysticercosis is the infection of the nervous system by the larvae of Taenia solium （T. solium）. Despite continuous effort, the experimental diagnosis of neurocysticercosis remains unresolved. Since the cerebrospinal fluid （CSF） contacts with the brain, dynamic information about pathological processes of the brain is likely to be reflected in CSF. Therefore, CSF may serve as a rich source of putative biomarkers related to neurocysticercosis. Comparative proteomic analysis of CSF of neurocysticercosis patients and control subjects may find differentially expressed proteins.Methods Two-dimensional difference in gel electrophoresis （2D-DIGE） was used to investigate differentially expressed proteins in CSF of patients with neurocysticercosis by comparing the protein profile of CSF from neurocysticercosis patients with that from control subjects. The differentially expressed spots/proteins were recognized with matrix-assisted laser desorption/ionization-time of flight-time of flight （MALDI-TOF-TOF） mass spectrometry.Results Forty-four enzyme digested peptides were obtained from 4 neurocysticercotic patients. Twenty-three were identified through search of the NCBI protein database with Mascot software, showing 19 up-expressed and 4 down-expressed. Of these proteins, 26S proteosome related to ATP- and ubiquitin-dependent degradation of proteins and lipocalin type prostaglandin D synthase involved in PGD2-synthesis and extracellular transporter activities were up-expressed, while transferrin related to iron metabolism within the brain was down-expressed.Conclusions This study established the proteomic profile of pooled CSF from 4 patients with neurocysticercosis, suggesting the potential value of proteomic analysis for the study of candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.展开更多
Background:The echinococcosis is prevalent in 10 provinces/autonomous region in western and northern China.Epidemiological survey of echinococcosis in China in 2012 showed the average prevalence of four counties in Ti...Background:The echinococcosis is prevalent in 10 provinces/autonomous region in western and northern China.Epidemiological survey of echinococcosis in China in 2012 showed the average prevalence of four counties in Tibet Autonomous Region(TAR)is 4.23%,much higher than the average prevalence in China(0.24%).It is important to understand the transmission risks and the prevalence of echinococcosis in human and animals in TAR.Methods:A stratified and proportionate sampling method was used to select samples in TAR.The selected residents were examined by B-ultrasonography diagnostic,and the faeces of dogs were tested for the canine coproantigen against Echinococcus spp.using enzyme-linked immunosorbent assay.The internal organs of slaughtered domestic animals were examined by visual examination and palpation.The awareness of the prevention and control of echinococcosis among of residents and students was investigated using questionnaire.All data were inputted using double entry in the Epi Info database,with error correction by double-entry comparison,the statistical analysis of all data was processed using SPSS 21.0,and the map was mapped using ArcGIS 10.1,the data was tested by Chi-square test and Cochran-Armitage trend test.Results:A total of 80384 people,7564 faeces of dogs,and 2103 internal organs of slaughtered domestic animals were examined.The prevalence of echinococcosis in humans in TAR was 1.66%,the positive rate in females(1.92%)was significantly higher than that in males(1.41%),(X2=30.31,P<0.01),the positive rate of echinococcosis was positively associated with age(X2trend=-423.95,P<0.01),and the occupational populations with high positive rates of echinococcosis were herdsmen(3.66%)and monks(3.48%).The average positive rate of Echinococcus coproantigen in TAR was 7.30%.The positive rate of echinococcosis in livestock for the whole region was 11.84%.The average awareness rate of echinococcosis across the region was 33.39%.Conclusions:A high prevalence of echinococcosis is found across the TAR,representing a very serious concern to human health.Efforts should be made to develop an action plan for echinococcosis prevention and control as soon as possible,so as to control the endemic of echinococcosis and reduce the medical burden on the population.展开更多
Summary What is already known about this topic?Echinococcosis,also known as hydatid disease,is a zoonotic parasitic disease caused by the larvae of Echinococcus granulosus.Western China has one of the most severe epid...Summary What is already known about this topic?Echinococcosis,also known as hydatid disease,is a zoonotic parasitic disease caused by the larvae of Echinococcus granulosus.Western China has one of the most severe epidemics worldwide.Echinococcosis is endemic in 370 counties of 9 provincial-level administrative divisions(PLADs)including Inner Mongolia,Sichuan,Yunnan,Tibet,Shaanxi,Gansu,Qinghai,Ningxia,Xinjiang and Xinjiang Production and Construction Corps(XPCC).展开更多
Since the first discovery of cyanobacterian plasmids in 1973, more than 60 strains have been found to have extra-chromosomal DNA, and their distribution covers almost all groups of the cyanobacteria. However, they wer...Since the first discovery of cyanobacterian plasmids in 1973, more than 60 strains have been found to have extra-chromosomal DNA, and their distribution covers almost all groups of the cyanobacteria. However, they were not discovered in the two unicellular genera Gloeocapsa and Gloeothece, and no further report was there on them ever since. Some species of these two genera having the ability to fix atmospheric nitrogen aerobically carry展开更多
文摘Background Soil-transmitted helminths(STHs)were previously endemic in Shandong Province,China.This study aimed to analyze the STHs prevalence trend and the natural,social,and human cognitive and behavioural factors infuencing the discrepancies between high and low infection levels from 2016 to 2020 in Shandong Province in eastern China.Methods STHs surveillance data of Shandong Province from 2016 to 2020 were obtained from China Information Management System for Prevention and Control of Parasitic Diseases.STHs infections were detected by modifed Kato–Katz method.Comprehensive information on the natural and social factors,STHs-related knowledge and behaviours were collected through questionnaire surveys.Retrospective spatial scan analysis was performed using SaTScan v10.1 to evaluate any identifed spatial clusters of STHs infection for statistical signifcance and Bayes discriminant analysis was used to discriminate the high or low infection groups of the villages.Results In total,72,160 participants were involved in our survey from 2016 to 2020.The overall STHs prevalence rate was 1.13%,with the eastern region of Shandong Province having the highest rate(2.02%).The predominant species was T.trichiura,with the prevalence rate of 0.99%and the≥70-year age group possessed the highest rate of 2.21%.The STHs prevalence rate showed an annual linear downward trend from 2016 to 2020(χ_(trend)^(2) =127.600,P<0.001).Respondents aged≥60 years had the lowest awareness level of STHs-related prevention knowledge(all P<0.05),and were the most likely to adopt the practice of fertilizing with fresh stool(χ^(2)=28.354,P<0.001).Furthermore,the southern region demonstrated the highest temperature and rainfall level and the lowest GNP and annual net income per capita(all P<0.05).Conclusions There is a remarkable declining in STHs prevalence in Shandong Province from 2016 to 2020.However,the prevalence rates of STHs especially T.trichiura in the southern and eastern regions were still high,and the elderly were more susceptible to be infected with STHs owning to their low awareness level of STHS-related prevention knowledge and high adoption rate of dangerous production and living behaviours.Integrated approaches of health education,environment improvement and behaviour change should be strengthened to obtain a further reduction of STHs prevalence in China.
基金Supported by the National Natural Science Foundation of China(No.31072125 and 31272549)by a National Science and Technology Project (China) grant (2008ZX-10004-011)
文摘Objective: To investigate the azole susceptibility of Candida albicans(C.albicans)from vulvovaginal candidosis patients and to analyze the relationship between ERG11 gene mutations in these isolates and azole resistance.Methods: Three hundred and two clinical isolates of Candida species were collected.Azole susceptibility was tested in vitro in microdilution studies. The ERG11 genes of 17 isolates of C. albicans(2 susceptibles, 5 dose-dependent resistants and 10 resistants) were amplified and sequenced.Results: Of the 302 isolates collected, 70.2% were C. albicans, of which 8.5%, 3.8% and4.2% were resistant to fluconazole, itraconazole and voriconazole, respectively. In total,27 missense mutations were detected in ERG11 genes from resistant/susceptible dosedependent isolates. Among them, Y132 H, A114 S, and Y257 H substitutions were most prevalent and were known to cause fluconazole resistance. G464 S and F72 S also have been proved to cause fluconazole resistance. Two novel substitutions(T285A, S457P) in hotspot regions were identified.Conclusions: Twenty seven mutations in the ERG11 gene were identified in azoleresistant C. albicans isolates, which indicated a possible relation with the increase in resistance to azole drugs and the recurrence of vulvovaginal candidosis. The relationship of two novel substitutions(T285A, S457P) with fluconazole resistance needs to be further verified by site-directed mutagenesis.
文摘China used to be one of the most heavily endemic countries for lymphatic filariasis(LF)in the world.There were 864 endemic counties/cities in 16 provinces/autonomous regions/municipalities(P/A/M)with a total population of 330 million at risk of infection.Since the founding of the People’s Republic of China in 1949,the Chinese Government has designated the control of the disease to be a top priority.Due to decades of sustained efforts,close cooperation related to LF control among government departments,and active participation of endemic populations,an all-round campaign for prevention and control has been carried out vigorously and successfully.Over many years,great achievements have been made through persistent endeavors of Chinese scientists and disease control workers.The ultimate goal to eliminate LF in the country was achieved in 2006.
文摘Background Neurocysticercosis is the infection of the nervous system by the larvae of Taenia solium （T. solium）. Despite continuous effort, the experimental diagnosis of neurocysticercosis remains unresolved. Since the cerebrospinal fluid （CSF） contacts with the brain, dynamic information about pathological processes of the brain is likely to be reflected in CSF. Therefore, CSF may serve as a rich source of putative biomarkers related to neurocysticercosis. Comparative proteomic analysis of CSF of neurocysticercosis patients and control subjects may find differentially expressed proteins.Methods Two-dimensional difference in gel electrophoresis （2D-DIGE） was used to investigate differentially expressed proteins in CSF of patients with neurocysticercosis by comparing the protein profile of CSF from neurocysticercosis patients with that from control subjects. The differentially expressed spots/proteins were recognized with matrix-assisted laser desorption/ionization-time of flight-time of flight （MALDI-TOF-TOF） mass spectrometry.Results Forty-four enzyme digested peptides were obtained from 4 neurocysticercotic patients. Twenty-three were identified through search of the NCBI protein database with Mascot software, showing 19 up-expressed and 4 down-expressed. Of these proteins, 26S proteosome related to ATP- and ubiquitin-dependent degradation of proteins and lipocalin type prostaglandin D synthase involved in PGD2-synthesis and extracellular transporter activities were up-expressed, while transferrin related to iron metabolism within the brain was down-expressed.Conclusions This study established the proteomic profile of pooled CSF from 4 patients with neurocysticercosis, suggesting the potential value of proteomic analysis for the study of candidate biomarkers involved in the diagnosis or pathogenesis of neurocysticercosis.
基金The survey funds are from public health project of central government transfer payment of China.
文摘Background:The echinococcosis is prevalent in 10 provinces/autonomous region in western and northern China.Epidemiological survey of echinococcosis in China in 2012 showed the average prevalence of four counties in Tibet Autonomous Region(TAR)is 4.23%,much higher than the average prevalence in China(0.24%).It is important to understand the transmission risks and the prevalence of echinococcosis in human and animals in TAR.Methods:A stratified and proportionate sampling method was used to select samples in TAR.The selected residents were examined by B-ultrasonography diagnostic,and the faeces of dogs were tested for the canine coproantigen against Echinococcus spp.using enzyme-linked immunosorbent assay.The internal organs of slaughtered domestic animals were examined by visual examination and palpation.The awareness of the prevention and control of echinococcosis among of residents and students was investigated using questionnaire.All data were inputted using double entry in the Epi Info database,with error correction by double-entry comparison,the statistical analysis of all data was processed using SPSS 21.0,and the map was mapped using ArcGIS 10.1,the data was tested by Chi-square test and Cochran-Armitage trend test.Results:A total of 80384 people,7564 faeces of dogs,and 2103 internal organs of slaughtered domestic animals were examined.The prevalence of echinococcosis in humans in TAR was 1.66%,the positive rate in females(1.92%)was significantly higher than that in males(1.41%),(X2=30.31,P<0.01),the positive rate of echinococcosis was positively associated with age(X2trend=-423.95,P<0.01),and the occupational populations with high positive rates of echinococcosis were herdsmen(3.66%)and monks(3.48%).The average positive rate of Echinococcus coproantigen in TAR was 7.30%.The positive rate of echinococcosis in livestock for the whole region was 11.84%.The average awareness rate of echinococcosis across the region was 33.39%.Conclusions:A high prevalence of echinococcosis is found across the TAR,representing a very serious concern to human health.Efforts should be made to develop an action plan for echinococcosis prevention and control as soon as possible,so as to control the endemic of echinococcosis and reduce the medical burden on the population.
文摘Summary What is already known about this topic?Echinococcosis,also known as hydatid disease,is a zoonotic parasitic disease caused by the larvae of Echinococcus granulosus.Western China has one of the most severe epidemics worldwide.Echinococcosis is endemic in 370 counties of 9 provincial-level administrative divisions(PLADs)including Inner Mongolia,Sichuan,Yunnan,Tibet,Shaanxi,Gansu,Qinghai,Ningxia,Xinjiang and Xinjiang Production and Construction Corps(XPCC).
文摘Since the first discovery of cyanobacterian plasmids in 1973, more than 60 strains have been found to have extra-chromosomal DNA, and their distribution covers almost all groups of the cyanobacteria. However, they were not discovered in the two unicellular genera Gloeocapsa and Gloeothece, and no further report was there on them ever since. Some species of these two genera having the ability to fix atmospheric nitrogen aerobically carry
