[Objective] To investigate the optimal determination conditions of melamine in animal blood products by high performance liquid chromatography （HPLC）. [ Method] The blood samples were extracted with ultrasonic in 1%...[Objective] To investigate the optimal determination conditions of melamine in animal blood products by high performance liquid chromatography （HPLC）. [ Method] The blood samples were extracted with ultrasonic in 1% trichloroacetic acid （TDA） and acetonitrile. After purifying by solide phase extraction （SPE）, the samples were analyzed by H PLC. r Result I The optimal conditions of HPLC were as follows： the chromatographic column was Zorbax SB-CS; the mobile phase was ion-pairs buffer-acetonitrile （95/5, V/V） ; the flow rate was 1.0 ml/min; the column temperature was 25 ℃ and the UV detection wavelength was 235 nm. The determined melamine concentration range was 0.001 -0.050 mg/ml; the linear correlation coefficient was 0.999 4; the concentration limit of melamine was 0.1 mg/kg; the average recovery rate of the melamine were 97.60% - 100.65%, and the relative standard deviation （RSD） was 1.23% -3.04%.[ Conclusion] The HPLC is simple, accurate and repeatable for determination of the melamine in animal blood products.展开更多
基金supported by the Shanghai Key Development Project of Agriculture Science and Technology (2009 No.6-3)
文摘[Objective] To investigate the optimal determination conditions of melamine in animal blood products by high performance liquid chromatography （HPLC）. [ Method] The blood samples were extracted with ultrasonic in 1% trichloroacetic acid （TDA） and acetonitrile. After purifying by solide phase extraction （SPE）, the samples were analyzed by H PLC. r Result I The optimal conditions of HPLC were as follows： the chromatographic column was Zorbax SB-CS; the mobile phase was ion-pairs buffer-acetonitrile （95/5, V/V） ; the flow rate was 1.0 ml/min; the column temperature was 25 ℃ and the UV detection wavelength was 235 nm. The determined melamine concentration range was 0.001 -0.050 mg/ml; the linear correlation coefficient was 0.999 4; the concentration limit of melamine was 0.1 mg/kg; the average recovery rate of the melamine were 97.60% - 100.65%, and the relative standard deviation （RSD） was 1.23% -3.04%.[ Conclusion] The HPLC is simple, accurate and repeatable for determination of the melamine in animal blood products.
