Expression level of miR-155 in peripheral blood

Objective:To investigate the relationship between the expression level of miR-155 and the severity of coronary lesion,and explore the action mechanism.Methods:Peripheral blood mononuclear cells(PBMC) were isolated form blood simple from patients with acute myocardial infarction(AMI),unstable angina(UAP).stable angina(SAP) and chesl pain syndrome(CPS).RT-PCR was performed to analysis the expression level of miR-155 in peripheral blood mononuclear cells,plasma and RAW264.7 macrophagocyte.MTT was used to analyze the cell viability of OxLDL treated RAW264.7 macrophagocyte.Results:The expression level of miR-155 in blood sample from coronary heart disease patients was much lower than in the blood sample of non-coronary heart disease(P<0.05).The level of miR-155 in PBMCs was much higher in the blood sample from CPS group than the other three group,and the level of miR-155 in plasma was higher in the CPS group than in the UAP and the AMI group,the difference was statistically significant(P<0.05).The expression level of miR-155 in PBMCs is positively associated with the level in the plasma(r=0.861,P=0.000).OxLDL can induce the expression of miR-155 in RAW264.7 macrophagocyte.decrease the cell viability of RAW264.7 macrophagocyte.and with the concentration and the treatment time of OxLDL increased,the effort become more obvious.The inhibition effort of OxLDL to RAW264.7macrophagocyte with high miR-155 expression is much lower than the control group,and it is statistically significant after treated for 12.24 and 48 h.Conclusions:miR-155 plays a protective role in the progression of atherosclerosis,and it may be achieved by reducing the apoptosis effort of OxLDL to RAW264.7 macrophagocyte.

INFLUENCE OF ACUPUNCTURE ON INSULIN RESISTANCE IN PATIENTS WITH SIMPLE OBESITY

Objective To investigate clinical therapeutic effect and regulative function of acupuncture on insulin resistance in patients with simple obesity. Methods Acupuncture was applied at the eight points surrounding the umbilicus: bilateral Tianshu (天枢ST 25), Huaroumen (滑肉门ST 24) and Wailing (外陵ST 26), Yinjiao (阴交CV 7) and Shuifen (水分CV 9), and also Guanyuan (关元CV 4) and Zusanli (足三里ST 36), and the ear embedding method was used in combination in 50 cases of simple obesity. The changes in FBG, INS, ISI, TC, TG, LDL-C and HDL-C after the treatment were observed. The changes in waistline (WL) and hip circumference (HC), their ratio (WL/HC), body weight (BW) and body mass index (BMI) after the treatment were also observed, and the therapeutic effects were compared between the patients with obesity in Grade Ⅰ and Grade Ⅱ. The aforementioned values were compared with those in the control group comprised of 30 normal subjects. Results Hyperinsulinemia (HI) appeared in the patients with simple obesity, and the value of ISI was obviously decreased, compared with that in normal subjects ( P<0.01 ). After the treatment, the values of WL, HC, WL/HC, BW and BMI were all obviously decreases ( P<0.05 ), and those of FBG, INS, TC, TG and LDL-C also reduced ( P<0.01 ), but those of ISI and HDL-C obviously increased ( P<0.05 ). Conclusion Acupuncture can not only efficiently improve the parameters in body fat in the patients with simple obesity, but also regulate their abnormal lipid metabolism and improve the situation of insulin resistance.

Estrogen receptor gene polymorphisms and bone mineral density in Chinese postmenopausal women

Objective To investigate the relationships between the polymorphisms of estrogen receptor (ER) gene, bone mineral density (BMD) and bone biochemical markers in Chinese postmenopausal women. Methods BMD of lumbar spine and femoral neck were measured using dual-energy X-ray absorptiometry (DEXA)in 186 Chinese postmenopausal women. The PvuⅡ and XbaⅠ polymorphisms of the ER gene were detected using polymerase chain reaction (PCR). Bone biochemical markers, serum alkaline phosphatase, osteocalcin and pyridinoline were measured by ELISA. Results The femoral neck(FN) BMD (Z score) was higher in pp compared to Pp (-0.01±0.12 vs. -0.35±0.09, P<0.05) while lumbar spine BMD (Z score) was higher in XX type compared to Xx and xx genotypes (0.01±0.45 vs -1.53±0.17, -1.29±0.10, P<0.001 and 0.001, respectively). Women without Px haplotype (n=79) had a higher BMD Z-score for the lumbar spine (-1.03±0.14 vs -1.45±0.11, P<0.05) and femoral neck (-0.01±0.11 vs -0.31±0.09, P<0.05) than those who had it (n=107). Conclusions The present study suggested that the pp and XX genotypes of ER gene might play a certain role in maintaining FN and lumbar spine BMD. ER genotypes without Px haplotype might be favorable to bone mass, while those with it might exert some harmful effect on bone mineral density.

Influence of Acupuncture on Insulin Resistance in Simple Obesity Patients

Objective： To investigate the regulatory effect of acupuncture on insulin resistance in simple obesity patients and its clinical effect. Methods： By the method ＂to benefit qi, strengthen the spleen, dissolve phlegm and remove turbidity＂, eight periumbilical acupoints, Tianshu （ST 25）, Huaroumen （ST 24）, Wailing （ST 26）, Yinjiao （CV 7） and Shuifen （CV 9）, and Guanyuan （CV 4） and Zusanli （ST 36） were used, in cooperation with ear-points-embedding method, and together with 30 healthy persons for comparison, in order to observe the changes in FBG, INS, ISI, TC, TG, LDLC and HDLC and to observe the changes in the waist circumference, hip circumference, waist-hip ratio, body weight and BMI in simple obesity patients before and after acupuncture treatments, and to observe the differences of the therapeutic effects between grade Ⅰ and grade Ⅱ obesity. Results： Hyperinsulinemia （HI） existed in the patients with simple obesity before the treatment, and ISI significantly decreased （P〈0.01） in comparison with the healthy group, and waist circumference, hip circumference, waist-hip ratio, body weight and BMI significantly decreased （P〈0.05, P〈0.01）, and FBG, INS, TC, TG and LDLC also significantly decreased （P〈0.01, P〈0.05）, and ISI and HDLC significantly increased （P〈0.05） after the treatment. Conclusion： Acupuncturecan not only effectively improve the body fat parameters but also regulate the abnormal fat metabolism and improve insulin resistance status in simple obesity patients.