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Immunomodulated signaling in macrophages: Studies on activation of Raf-1, MAPK, cPLA_2 and secretion of IL-12 被引量:5
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作者 张宗梁 宋秋宝 +3 位作者 林明群 丁跃梅 康小伟 姚錱 《Science China(Life Sciences)》 SCIE CAS 1997年第6期583-592,共10页
Little is known about the mechanism and signal transduction by LPS-mediated immunomodulation of murine peritoneal macrophages. It is found that the signal molecules of the down-stream of Ras, Raf-1, MAPK p44, and MAPK... Little is known about the mechanism and signal transduction by LPS-mediated immunomodulation of murine peritoneal macrophages. It is found that the signal molecules of the down-stream of Ras, Raf-1, MAPK p44, and MAPK p42 are phosphorylated, and cPLA2 is activated with a significant increase of the release of [ H3 ] AA by macrophages in response to LPS and PMA. Compared with the very recent finding that LPS and PMA trigger the activation and translocation of PKC-α and PKC-ε, these findings suggest that there is a connection between PKC signaling pathway and the Raf-1/MAPK pathway and that the activation of these main signaling events may be closely related to the secretion of IL-12 during LPS-induced modulation of macrophages. 展开更多
关键词 immunomodulation of murine suppressor macrophages signal transduction PKC isoforms/Raf-1 /MAPK/cPLA_(2). IL-12 secretion.
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Comparison of dark- and light-adapted carp retinas with NADPH diaphorase staining 被引量:1
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作者 叶冰 杨雄里 《Science China(Life Sciences)》 SCIE CAS 1996年第3期251-257,共7页
The carp retina was examined by NADPH diaphorase histochemistry to determine if the staining pattern of retinal cells was changed depending on the adaptation state of the retina. When dark-adapted for 5 h, ellipsoids ... The carp retina was examined by NADPH diaphorase histochemistry to determine if the staining pattern of retinal cells was changed depending on the adaptation state of the retina. When dark-adapted for 5 h, ellipsoids of inner segments of both rods and cones and some horizontal cells were heavily stained. Staining was also found in subpopulations of amacrine cells and ganglion cells. In addition, Muller cells were strongly positive for NADPH diaphorase. When light-adapted for 5h, ellipsoids of photoreceptors and ganglion cells were less intensely stained, whereas Muller cells and horizontal cells became negative for NADPH diaphorase. Furthermore, rod ON-center bipolar cells were clearly stained. The difference of staining of amacrine cells between dark- and light-adapted retinas was not significant. The differences in diaphorase-staining pattern between dark- and light-adapted retinas suggest that Muller cells, some horizontal cells and rod ON-center bipolar cells contain inducible nitric oxide synthase, whose induction depends on the adaptation state. 展开更多
关键词 retinal cells NADPH DIAPHORASE adaptation NITRIC oxide synthase.
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Differential effects of nitric oxide on rod and cone pathways in carp retina
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作者 叶冰 杜久林 杨雄里 《Science China(Life Sciences)》 SCIE CAS 1997年第1期71-78,共8页
The effects of nitric oxide (NO) on electroretinograms and light responses of horizontal cells intra-cellularly recorded from isolated, superfused carp retinas were studied. Sodium nitroprusside (SNP), an NO donor, su... The effects of nitric oxide (NO) on electroretinograms and light responses of horizontal cells intra-cellularly recorded from isolated, superfused carp retinas were studied. Sodium nitroprusside (SNP), an NO donor, suppressed scotopic b wave, while enhancing photopic b wave, and the effects could be blocked by hemoglobin, an NO chelator. Furthermore, following SNP application, light responses of rod horizontal cells were reduced in size and those of cone horizontal cells were increased. These results suggest that NO suppresses the activity of rod pathway, but enhances that of cone pathway in the outer retina. Moreover, the effects of methylene blue, an inhibitor of soluble guanylate cyclase, on rod and cone horizontal cells were just opposite to those of SNP, implying that the effects of NO may be mediated by cGMP. 展开更多
关键词 NITRIC oxide ROD PATHWAY CONE PATHWAY ELECTRORETINOGRAM (ERG) horizontal cell.
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Characterization of S1 nuclease sensitive site at transcription initiation region of Attacus ricini rDNA
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作者 何明亮 赵慕钧 +1 位作者 靳嘉瑞 李载平 《Science China(Life Sciences)》 SCIE CAS 1997年第4期398-405,共8页
A single-stranded S1 nuclease hypersensitive site which contains a d(AT)18 sequence structure locat-ed in the 5 -non transcription spacer of silkworm A . ricini ribosomal RNA gene has been reported[1] Using starved-re... A single-stranded S1 nuclease hypersensitive site which contains a d(AT)18 sequence structure locat-ed in the 5 -non transcription spacer of silkworm A . ricini ribosomal RNA gene has been reported[1] Using starved-refed silkworms, another S1 nuclease sensitive site was found existing in the rDNA chromatin, while under merely starving, this S1 sensitive site disappeared[2] . Recently this inducible S1 sensitive site has been further determined. It consists of a d(GT)10-d(AT)10 special DNA sequence at the transcription initiation region, and shows a behavior of ease in DNA-unwinding, indicating that S1 nuclease sensitive sites may have an important function in the regulation of rDNA transcription and replication. 展开更多
关键词 S1 NUCLEASE sensitive SITE RIBOSOMAL RNA gene DNA-unwinding element. SOUTHERN hybridiza-
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