BACKGROUND Ulcerative colitis(UC)is a chronic,nonspecific intestinal inflammatory disease.Acupuncture and moxibustion is proved effective in treating UC,but the mechanism has not been clarified.Proteomic technology ha...BACKGROUND Ulcerative colitis(UC)is a chronic,nonspecific intestinal inflammatory disease.Acupuncture and moxibustion is proved effective in treating UC,but the mechanism has not been clarified.Proteomic technology has revealed a variety of biological markers related to immunity and inflammation in UC,which provide new insights and directions for the study of mechanism of acupuncture and moxibustion treatment of UC.AIM To investigate the mechanism of electroacupuncture(EA)and herb-partitioned moxibustion(HM)on UC rats by using proteomics technology.METHODS Male Sprague-Dawley rats were randomly divided into the normal(N)group,the dextran sulfate sodium(DSS)-induced UC model(M)group,the HM group,and the EA group.UC rat model was prepared with 3%DSS,and HM and EA interventions at the bilateral Tianshu and Qihai acupoints were performed in HM or EA group.Haematoxylin and eosin staining was used for morphological evaluation of colon tissues.Isotope-labeled relative and absolute quantification(iTRAQ)and liquid chromatography-tandem mass spectrometry were performed for proteome analysis of the colon tissues,followed by bioinformatics analysis and protein-protein interaction networks establishment of differentially expressed proteins(DEPs)between groups.Then western blot was used for verification of selected DEPs.RESULTS The macroscopic colon injury scores and histopathology scores in the HM and EA groups were significantly decreased compared to the rats in the M group(P<0.01).Compared with the N group,a total of 202 DEPs were identified in the M group,including 111 up-regulated proteins and 91 down-regulated proteins,of which 25 and 15 proteins were reversed after HM and EA interventions,respectively.The DEPs were involved in various biological processes such as biological regulation,immune system progression and in multiple pathways including natural killer cell mediated cytotoxicity,intestinal immune network for immunoglobulin A(IgA)production,and FcγR-mediated phagocytosis.The Kyoto Encyclopedia of Genes and Genomes pathways of DEPs between HM and M groups,EA and M groups both included immuneassociated and oxidative phosphorylation.Network analysis revealed that multiple pathways for the DEPs of each group were involved in protein-protein interactions,and the expression of oxidative phosphorylation pathway-related proteins,including ATP synthase subunit g(ATP5L),ATP synthase beta subunit precursor(Atp5f),cytochrome c oxidase subunit 4 isoform 1(Cox4i1)were down-regulated after HM and EA interventions.Subsequent verification of selected DEPs(Synaptic vesicle glycoprotein 2A;nuclear cap binding protein subunit 1;carbamoyl phosphate synthetase 1;Cox4i1;ATP synthase subunit b,Atp5f1;doublecortin like kinase 3)by western blot confirmed the reliability of the iTRAQ data,HM and EA interventions can significantly downregulate the expression of oxidative phosphorylation-associated proteins(Cox4i1,Atp5f1)(P<0.01).CONCLUSION EA and HM could regulate the expression of ATP5L,Atp5f1,Cox4i1 that associated with oxidative phosphorylation,then might regulate immune-related pathways of intestinal immune network for IgA production,FcγR-mediated phagocytosis,thereby alleviating colonic inflammation of DSSinduced UC rats.展开更多
Women worldwide are dealing with hot flashes (潮热), a perimenopausal feature that is often tiring,embarrassing, and energeticallydraining. Conventionally, Chinese medicine defines hot flashes as Kidney-yin deficiency...Women worldwide are dealing with hot flashes (潮热), a perimenopausal feature that is often tiring,embarrassing, and energeticallydraining. Conventionally, Chinese medicine defines hot flashes as Kidney-yin deficiency and its clinical manifestations include red face,sweating, hot sensations in the palms and soles, mouth and nose dryness, constipation, insomnia, lumbar soreness, knee weakness, redtongue, etc. To obtain a broader perspective and understand the dynamics of hot flashes, we examined the mechanisms behind hot flashesbased on both the knowledge from ancient Chinese medicinal texts, as well as novel research findings of Chinese and Western medicine.This perspective was the foundation for the acupuncture study of our traditional Chinese medicine conducted in 2018-2019 in Shanghai.This study, designed as a pragmatic randomized control trial with two parallel groups, focused on regulating and unblocking conceptionand governor vessels. The results confirmed that our acupuncture method could effectively reduce both the frequency and severity of hotflashes and improve life quality of middle-aged women.展开更多
Taiji studies are a field of study that has its roots in the philosophical thought of the Yi Jing or Book of changes.It integrates the self‑cultivation traditions of Confucianism,Buddhism,and Daoism,and centers its ac...Taiji studies are a field of study that has its roots in the philosophical thought of the Yi Jing or Book of changes.It integrates the self‑cultivation traditions of Confucianism,Buddhism,and Daoism,and centers its academic system mostly on the perspective of the“states view”(jing jie guan).From the standpoint of its philosophical theory,Taiji studies divide the understanding of Dao into three theoretical states:the state of existence(you jie),the state of existence‑nonexistence(you wu jie),and the state of nonexistence(wu jie).It also establishes a theoretical structure that mainly includes“three states and nine axioms,”“One Dao and Nine theory sections,”and“Three practice levels and Nine secrets.”Based on traditional Chinese culture and philosophy,Taiji studies are continuously integrating the essence of them for better understanding and raising,and finally set up a rational school of Taiji Da Dao。展开更多
Acupuncture has been used for 1000 years, but how it gives therapeutic effect is not completely understood. Integration of brain wave as the central nervous system plays an important role in acupuncture efficacy. To e...Acupuncture has been used for 1000 years, but how it gives therapeutic effect is not completely understood. Integration of brain wave as the central nervous system plays an important role in acupuncture efficacy. To explore the effect of acupuncture stimulation in the central nervous system, it is imperative to decide the adjustments in brain waves that produced because of acupuncture stimulation. Electroencephalogram(EEG) is often used in the acupuncture research area. EEG records spontaneous cerebral activity after some time by positioning electrodes on scalp surface. Using EEG, we can observe the brain connectivity regarding the acupuncture stimulation. An overview regarding the effect of manual acupuncture on human brain wave through EEG observation will be discussed in this review.展开更多
Objective: To evaluate the efficacy and safety of essential oil treatment for type Ⅲ chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS). Methods: A randomized controlled trial was conducted from December 2014 ...Objective: To evaluate the efficacy and safety of essential oil treatment for type Ⅲ chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS). Methods: A randomized controlled trial was conducted from December 2014 to October 2015. Seventy type Ⅲ CP/CPPS patients were assigned to the essential oil group(35 cases) or almond placebo oil control group(35 cases) by a random number table. The oil was smeared by self-massage on the suprapubic and sacral region once a day for 4 weeks. The National Institutes of Health Chronic Prostatitis Syndrome Index(NIH-CPSI) and expressed prostatic secretions(EPS) were examined. The primary outcome was NIH-CPSI pain domain. The secondary outcomes included other NIH-CPSI domains and laboratory examinations of EPS. Adverse events were also observed. Results: Sixty-six subjects completed the full 4-week treatment. There was no significant difference between almond oil control and essential oil groups in terms of the total score of NIH-CPSI, pain, quality of life and urination domain scores of NIH-CPSI and EPS examinations(P>0.05). In the essential oil group, pain between rectum and testicles(perineum) in the domain of pain or discomfort was significantly reduced at week 2 and week 4 compared with almond oil control group(P<0.01). No serious adverse events occurred. Conclusion: The essential oil may reduce the pain or discomfort in the perineum region in patients with CP/CPPS.展开更多
Objective:To evaluate the effectiveness and safety of Chinese herbal external umbilicus treatment with Modified Dinggui Powder(加味丁桂散,MDGP)in patients with chronic nonbacterial prostatitis(CNP).Methods:A randomize...Objective:To evaluate the effectiveness and safety of Chinese herbal external umbilicus treatment with Modified Dinggui Powder(加味丁桂散,MDGP)in patients with chronic nonbacterial prostatitis(CNP).Methods:A randomized,double-blind,placebo-controlled clinical trial was conducted among 72 patients with CNP.Participants were randomly allocated to a treatment group and a placebo group using computer software in a 1:1 ratio,and received either MDGP external umbilicus treatment(MDGP group,36 cases)or placebo(control group,36 cases)at acupoints Shenque(CV 8),twice a week for 4 weeks.In addtion,patients all received herbal medicine treatment twice a day for 4 weeks.The primary outcome was the US National Institutes of Health Chronic Prostatitis Symptom Scores Index(NIH-CPSI)with a questionnaire at weeks 2 and 4.The secondary outcomes including prostatic fluid examination(white blood cells and lecithin bodies),the clinical efficacy evaluation,and the adverse events were also assessed during the entire trial.Results:The NIH-CPSI scores regarding pain or discomfort scores showed greater improvement in the MDGP group than placebo control group at weeks 2(P=0.001)and week 4(P=0.004),respectively.NIH-CPSI scores of symptom severity,total scores and leukocytes number in the prostatic fluid in the MDGP group were significantly improved(P<0.05).There was no statistical difference in the urinary symptoms,quality of life,lecithin and other scores between two groups(P>0.05).The clinical effective rate was 73.53%(25/34)in the MDGP group,which was significally higher than the placebo control group with 48.39%(25/31,P<0.05).Patients were blinded successfully,and no serious adverse effects were found during the trial.Conclusion:A 4-week course of umbilicus treatment with modified Dinggui Powder seems to relieve pain and symptom severity effectively and increase the amount of leukocytes number in patients with CNP(Trial registration No.ChiCTRI 800014687).展开更多
Objective:The objective of this study is to systematically review and analyze the efficacy of acupuncture for pain caused by primary liver cancer(PLC).Materials and Methods:We searched databases,including PubMed,Medli...Objective:The objective of this study is to systematically review and analyze the efficacy of acupuncture for pain caused by primary liver cancer(PLC).Materials and Methods:We searched databases,including PubMed,Medline,Embase,Cochrane Library,Web of Science,China National Knowledge Infrastructure,Chinese Science and Technology Periodicals Database(VIP),Wanfang,and SinoMed/Chinese Biomedical Database(CBM),and retrieved randomized controlled trials(RCTs)that used acupuncture as the primary intervention to treat pain caused by PLC.Two investigators then screened the articles,extracted and pooled data,and evaluated the risk of bias of the included articles according to the Cochrane Handbook.RevMan5.3 was used for the meta-analysis of eligible RCTs.Results:A total of 145 articles were retrieved;after screening,8 RCTs involving 496 patients were eventually included in this meta-analysis.The results showed that acupuncture effectively improved cancer pain and was superior to Western medicine.Moreover,acupuncture was fast-acting for pain relief,prolonged the relief,and prevented relapse.Its adverse reaction rate was also significantly lower than that of Western medicine.No significant difference was observed in Visual Analog Scale score between acupuncture and Western medicine.Conclusion:Acupuncture relieves pain caused by liver cancer and can be used as an adjunct and alternative therapy for drug treatment.The existing research evidence is not yet objective or comprehensive,and more rigorous clinical trials are needed to validate the results.展开更多
基金the National Natural Science Foundation of China No.81973955,82004475 and 82174501Shanghai Clinical Research Center for Acupuncture and Moxibustion No.20MC1920500+1 种基金Clinical Key Specialty Construction Foundation of Shanghai No.shslczdzk04701Natural Science Foundation of Shanghai No.21ZR1460200
文摘BACKGROUND Ulcerative colitis(UC)is a chronic,nonspecific intestinal inflammatory disease.Acupuncture and moxibustion is proved effective in treating UC,but the mechanism has not been clarified.Proteomic technology has revealed a variety of biological markers related to immunity and inflammation in UC,which provide new insights and directions for the study of mechanism of acupuncture and moxibustion treatment of UC.AIM To investigate the mechanism of electroacupuncture(EA)and herb-partitioned moxibustion(HM)on UC rats by using proteomics technology.METHODS Male Sprague-Dawley rats were randomly divided into the normal(N)group,the dextran sulfate sodium(DSS)-induced UC model(M)group,the HM group,and the EA group.UC rat model was prepared with 3%DSS,and HM and EA interventions at the bilateral Tianshu and Qihai acupoints were performed in HM or EA group.Haematoxylin and eosin staining was used for morphological evaluation of colon tissues.Isotope-labeled relative and absolute quantification(iTRAQ)and liquid chromatography-tandem mass spectrometry were performed for proteome analysis of the colon tissues,followed by bioinformatics analysis and protein-protein interaction networks establishment of differentially expressed proteins(DEPs)between groups.Then western blot was used for verification of selected DEPs.RESULTS The macroscopic colon injury scores and histopathology scores in the HM and EA groups were significantly decreased compared to the rats in the M group(P<0.01).Compared with the N group,a total of 202 DEPs were identified in the M group,including 111 up-regulated proteins and 91 down-regulated proteins,of which 25 and 15 proteins were reversed after HM and EA interventions,respectively.The DEPs were involved in various biological processes such as biological regulation,immune system progression and in multiple pathways including natural killer cell mediated cytotoxicity,intestinal immune network for immunoglobulin A(IgA)production,and FcγR-mediated phagocytosis.The Kyoto Encyclopedia of Genes and Genomes pathways of DEPs between HM and M groups,EA and M groups both included immuneassociated and oxidative phosphorylation.Network analysis revealed that multiple pathways for the DEPs of each group were involved in protein-protein interactions,and the expression of oxidative phosphorylation pathway-related proteins,including ATP synthase subunit g(ATP5L),ATP synthase beta subunit precursor(Atp5f),cytochrome c oxidase subunit 4 isoform 1(Cox4i1)were down-regulated after HM and EA interventions.Subsequent verification of selected DEPs(Synaptic vesicle glycoprotein 2A;nuclear cap binding protein subunit 1;carbamoyl phosphate synthetase 1;Cox4i1;ATP synthase subunit b,Atp5f1;doublecortin like kinase 3)by western blot confirmed the reliability of the iTRAQ data,HM and EA interventions can significantly downregulate the expression of oxidative phosphorylation-associated proteins(Cox4i1,Atp5f1)(P<0.01).CONCLUSION EA and HM could regulate the expression of ATP5L,Atp5f1,Cox4i1 that associated with oxidative phosphorylation,then might regulate immune-related pathways of intestinal immune network for IgA production,FcγR-mediated phagocytosis,thereby alleviating colonic inflammation of DSSinduced UC rats.
文摘Women worldwide are dealing with hot flashes (潮热), a perimenopausal feature that is often tiring,embarrassing, and energeticallydraining. Conventionally, Chinese medicine defines hot flashes as Kidney-yin deficiency and its clinical manifestations include red face,sweating, hot sensations in the palms and soles, mouth and nose dryness, constipation, insomnia, lumbar soreness, knee weakness, redtongue, etc. To obtain a broader perspective and understand the dynamics of hot flashes, we examined the mechanisms behind hot flashesbased on both the knowledge from ancient Chinese medicinal texts, as well as novel research findings of Chinese and Western medicine.This perspective was the foundation for the acupuncture study of our traditional Chinese medicine conducted in 2018-2019 in Shanghai.This study, designed as a pragmatic randomized control trial with two parallel groups, focused on regulating and unblocking conceptionand governor vessels. The results confirmed that our acupuncture method could effectively reduce both the frequency and severity of hotflashes and improve life quality of middle-aged women.
文摘Taiji studies are a field of study that has its roots in the philosophical thought of the Yi Jing or Book of changes.It integrates the self‑cultivation traditions of Confucianism,Buddhism,and Daoism,and centers its academic system mostly on the perspective of the“states view”(jing jie guan).From the standpoint of its philosophical theory,Taiji studies divide the understanding of Dao into three theoretical states:the state of existence(you jie),the state of existence‑nonexistence(you wu jie),and the state of nonexistence(wu jie).It also establishes a theoretical structure that mainly includes“three states and nine axioms,”“One Dao and Nine theory sections,”and“Three practice levels and Nine secrets.”Based on traditional Chinese culture and philosophy,Taiji studies are continuously integrating the essence of them for better understanding and raising,and finally set up a rational school of Taiji Da Dao。
文摘Acupuncture has been used for 1000 years, but how it gives therapeutic effect is not completely understood. Integration of brain wave as the central nervous system plays an important role in acupuncture efficacy. To explore the effect of acupuncture stimulation in the central nervous system, it is imperative to decide the adjustments in brain waves that produced because of acupuncture stimulation. Electroencephalogram(EEG) is often used in the acupuncture research area. EEG records spontaneous cerebral activity after some time by positioning electrodes on scalp surface. Using EEG, we can observe the brain connectivity regarding the acupuncture stimulation. An overview regarding the effect of manual acupuncture on human brain wave through EEG observation will be discussed in this review.
文摘Objective: To evaluate the efficacy and safety of essential oil treatment for type Ⅲ chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS). Methods: A randomized controlled trial was conducted from December 2014 to October 2015. Seventy type Ⅲ CP/CPPS patients were assigned to the essential oil group(35 cases) or almond placebo oil control group(35 cases) by a random number table. The oil was smeared by self-massage on the suprapubic and sacral region once a day for 4 weeks. The National Institutes of Health Chronic Prostatitis Syndrome Index(NIH-CPSI) and expressed prostatic secretions(EPS) were examined. The primary outcome was NIH-CPSI pain domain. The secondary outcomes included other NIH-CPSI domains and laboratory examinations of EPS. Adverse events were also observed. Results: Sixty-six subjects completed the full 4-week treatment. There was no significant difference between almond oil control and essential oil groups in terms of the total score of NIH-CPSI, pain, quality of life and urination domain scores of NIH-CPSI and EPS examinations(P>0.05). In the essential oil group, pain between rectum and testicles(perineum) in the domain of pain or discomfort was significantly reduced at week 2 and week 4 compared with almond oil control group(P<0.01). No serious adverse events occurred. Conclusion: The essential oil may reduce the pain or discomfort in the perineum region in patients with CP/CPPS.
基金supported by National Natural Science Foundation of China(No.81303029)National Basic Research Program of China(973 Program,No.2009CB522900)Natural Science Foundation of Shanghai(No.12ZR1429700)~~
基金supported by National Basic Research Program of China 973 Program(No.2015CB554501,No.2009CB522900)Shanghai Rising-star Program(No.16QA1403400)the Program for New Century Excellent Talents in University of Ministry of Education(No.NCET-13-0907)~~
基金supported by National Natural Science Foundation of China(No.81173331,No.81303033,No.81473758)the 3-year Action Plan for Traditional Chinese Medicine of Shanghai Municipal Health Bureau(No.ZYSNXD-CC-ZDYJ053)+1 种基金National Basic Research Program of China(973 Program,No.2015CB554500)Innovation Program of Shanghai Municipal Education Commission(No.2014YZ052)~~
基金supported by National Basic Research Program of China(973 Program,No.2015CB554501)Natural Science Foundation of Shanghai(No.13ZR1439400)+1 种基金Program for New Century Excellent Talents in University(No.NCET-13-0907)Leading Academic Discipline Project of Shanghai(No.JZ2012019)~~
基金the Shanghai Three-Year Development Plan Project for Traditional Chinese Medicine[No.ZY(2018-2020)-CCCX-2001-05]。
文摘Objective:To evaluate the effectiveness and safety of Chinese herbal external umbilicus treatment with Modified Dinggui Powder(加味丁桂散,MDGP)in patients with chronic nonbacterial prostatitis(CNP).Methods:A randomized,double-blind,placebo-controlled clinical trial was conducted among 72 patients with CNP.Participants were randomly allocated to a treatment group and a placebo group using computer software in a 1:1 ratio,and received either MDGP external umbilicus treatment(MDGP group,36 cases)or placebo(control group,36 cases)at acupoints Shenque(CV 8),twice a week for 4 weeks.In addtion,patients all received herbal medicine treatment twice a day for 4 weeks.The primary outcome was the US National Institutes of Health Chronic Prostatitis Symptom Scores Index(NIH-CPSI)with a questionnaire at weeks 2 and 4.The secondary outcomes including prostatic fluid examination(white blood cells and lecithin bodies),the clinical efficacy evaluation,and the adverse events were also assessed during the entire trial.Results:The NIH-CPSI scores regarding pain or discomfort scores showed greater improvement in the MDGP group than placebo control group at weeks 2(P=0.001)and week 4(P=0.004),respectively.NIH-CPSI scores of symptom severity,total scores and leukocytes number in the prostatic fluid in the MDGP group were significantly improved(P<0.05).There was no statistical difference in the urinary symptoms,quality of life,lecithin and other scores between two groups(P>0.05).The clinical effective rate was 73.53%(25/34)in the MDGP group,which was significally higher than the placebo control group with 48.39%(25/31,P<0.05).Patients were blinded successfully,and no serious adverse effects were found during the trial.Conclusion:A 4-week course of umbilicus treatment with modified Dinggui Powder seems to relieve pain and symptom severity effectively and increase the amount of leukocytes number in patients with CNP(Trial registration No.ChiCTRI 800014687).
基金supported by Key Project of Chinese National Programs for Fundamental Research and Development(973 Program)(No.2009CB522900,No.2015CB554501)the Project of Three-Year Action Plan for development of traditional Chinese medicine in Shanghai(No.ZY(2018-2020)-CCCX-2004-01)Shanghai Clinical Research Center for Acupuncture and Moxibustion(No.20MC1920500)。
文摘Objective:The objective of this study is to systematically review and analyze the efficacy of acupuncture for pain caused by primary liver cancer(PLC).Materials and Methods:We searched databases,including PubMed,Medline,Embase,Cochrane Library,Web of Science,China National Knowledge Infrastructure,Chinese Science and Technology Periodicals Database(VIP),Wanfang,and SinoMed/Chinese Biomedical Database(CBM),and retrieved randomized controlled trials(RCTs)that used acupuncture as the primary intervention to treat pain caused by PLC.Two investigators then screened the articles,extracted and pooled data,and evaluated the risk of bias of the included articles according to the Cochrane Handbook.RevMan5.3 was used for the meta-analysis of eligible RCTs.Results:A total of 145 articles were retrieved;after screening,8 RCTs involving 496 patients were eventually included in this meta-analysis.The results showed that acupuncture effectively improved cancer pain and was superior to Western medicine.Moreover,acupuncture was fast-acting for pain relief,prolonged the relief,and prevented relapse.Its adverse reaction rate was also significantly lower than that of Western medicine.No significant difference was observed in Visual Analog Scale score between acupuncture and Western medicine.Conclusion:Acupuncture relieves pain caused by liver cancer and can be used as an adjunct and alternative therapy for drug treatment.The existing research evidence is not yet objective or comprehensive,and more rigorous clinical trials are needed to validate the results.
基金supported by National Natural Science Foundation of China,No.81001547Shanghai Leading Academic Discipline Project,No.S30304Cultivation Fund for Young Teacher in Shanghai Colleges and Universities,No.ZZszy13052~~
