The immunophenotype, rearrangements of T cell receptor(TCR) γ andδchain genes as well as the immunoglobulinheavy chain (IgH)gene were studied in 37 cases ofmorphologically defined acute lymphoblastic leukemi...The immunophenotype, rearrangements of T cell receptor(TCR) γ andδchain genes as well as the immunoglobulinheavy chain (IgH)gene were studied in 37 cases ofmorphologically defined acute lymphoblastic leukemia (ALL).According to the expression of differentiation antigens, 8 caseswere classified as T-ALL, 26 B lineage ALL, 2 acute un-differentiated leukemia (AUL) and myeloid phenotype. An or-der of TCR gene rearrangements was observed in T-ALL,with the rearrangement of δgene preceding that of γgene.Both genes were also found frequently rearranged and / or de-leted in high proportions of the ALL of B cell lineage. Howev-er, the patterns of gene rearrangements were somewhat differ-ent between the T and B lineage ALLs. In contrast, the lgHgene rearrangements were observed only in the B lineage ALL.The immunogenotype analysis of ALL proved to be a usefulmarker of the clonality and provided us with important informa-tion on early human lymphoid differentiation. We concludethat the determination of T展开更多
基金This work was supported by grants from the Shanghai Foundation for Natural Sciences,the Ministry of Health,.for Young Researchers and the National Natural Sciences Foundation of China
文摘The immunophenotype, rearrangements of T cell receptor(TCR) γ andδchain genes as well as the immunoglobulinheavy chain (IgH)gene were studied in 37 cases ofmorphologically defined acute lymphoblastic leukemia (ALL).According to the expression of differentiation antigens, 8 caseswere classified as T-ALL, 26 B lineage ALL, 2 acute un-differentiated leukemia (AUL) and myeloid phenotype. An or-der of TCR gene rearrangements was observed in T-ALL,with the rearrangement of δgene preceding that of γgene.Both genes were also found frequently rearranged and / or de-leted in high proportions of the ALL of B cell lineage. Howev-er, the patterns of gene rearrangements were somewhat differ-ent between the T and B lineage ALLs. In contrast, the lgHgene rearrangements were observed only in the B lineage ALL.The immunogenotype analysis of ALL proved to be a usefulmarker of the clonality and provided us with important informa-tion on early human lymphoid differentiation. We concludethat the determination of T
