Nondestructive X-ray imaging of inner structure of soft tissues in phase contrast

An experimental study on nondestructive X-ray imaging of inner structure of soft tissues in phase con-trast has been conducted with Beijing Synchrotron Radiation Facility (BSRF). Modification to the beamline setupwas made to enlarge the X-ray beam section and consequently larger samples could be imaged. In-line setup was em-ployed for experiments. Results on a series of samples were given and soft-tissue details of less than 50 μm inside afresh goldfish were obtained. Diagnosis of tumor in its early stage was also investigated taking SD rats as the model.Tumor at the size of ～ 100μm was observed. Potential of this technique in clinic diagnosis was discussed.

Radiolabeling of N-Succinimidyl-3-[(125)~I]iodobenzoate intermediate

Organostannanes were important precursors which was easy to radioiodinate. N-Succinimidyl-3- (tri-n-butylstannyl) benzoate (STB) was radiolabeled using Iodogen to get radioactive N-Succinimidyl-3-iodobenzo- ate (S125IB) with 96% of high radiochemical yield. The optimization of labeling condition was explored in this study. S125IB was stable at room temperature in dark. Cold SIB was prepared as a standard and IR and NMR results were given in this article.

Avidin chase reduces side effects of radioimmunotherapy in nude mice bearing human colon carcinoma

AIM: To evaluate the influence of avidin chase on the side effects of radioimmunotherapy (RIT) in nude mice bearing human colon carcinoma and therapeutic outcome.METHODS: Purified anti-CEA monoclonal antibody (McAb)was biotinylated with NHS-biotin, and then radiolabeled with 188Re by the direct method. 188Re-labeledbiotinylated anti-CEA McAb (188Re-CEA McAb-Bt) was intravenously injected followed by intravenous injection of avidin after 24 h. SPECT imaging and biodistribution study were performed at 28-48 h after the injection of 188Re-CEA McAb-Bt. Three groups of nude mice subcutaneously grafted with human colon carcinoma were treated 7 d after the graft. Mice in the avidin chase group received intravenous injection of 188Re-CEA McAb-Bt (11.1 MBq/20 μg) followed by intravenous injection of cold avidin (80 μg) after 24 h. Mice in the control group (treated group without avidin chase) only received the injection of 188Re-CEA McAb-Bt (11.1 MBq/20 μg), another control group (non-treated group) only received 0.1 mL normal saline solution. Toxicity was evaluated on the basis of change of body weight and peripheral WBC counts, and therapy effects were determined by variation in tumor volume. Histological analysis of tumors was also performed.RESULTS: Avidin chase markedly accelerated the clearance of 188Re-CEA McAb-Bt from the blood and normal tissues. The tumor uptakes of 188Re-CEA Mc Ab-Bt at 28 h were 5.90 and 6.42% ID/g, respectively, in chase group and in non-chase group, while the tumor-to-background (T/NT) ratios were 3.19 and 0.56, respectively. The tumor uptake was slightly decreased by avidin chase, but the T/NT ratios were increased. In treated groups the growth rate of body weight and the number of WBC decreased after injection of 188Re-CEA McAb-Bt, and the WBC counts recovered earlier in the group with avidin chase than in the group without avidin chase. Compared to the nontreated group, treated groups with and without avidin chase showed significant anti-tumor effects.CONCLUSION: Avidin chase can effectively reduce the side effects of RIT, and improve therapeutic efficacy.

Induced ion-channel instability

In this paper, a new mechanism of electromagnetic instability, the induced ion-channel instability, is stud- ied. It is based on the transverse driven betatron oscillation of relativistic electron beam induced by an additional magnetic undulator with a period close to the betatron wavelength in an ion channel. As its amplitude is sensitive to the electron beam energy, the driven betatron oscillation may determine electron beam grouping in the ponderomotive potential by selecting the undulator strength and period, and it provides a new mechanism of electron bunching, re- sulting in electromagnetic instability. Under proper condition, a new free-electron laser based on this mechanism may be realized.

Recent progress of nuclear liquid gas phase transition

Recent progress on nuclear liquid gas phase transition (LGPT) has been reviewed, especially for the signals of LGPT in heavy ion collisions. These signals include the power-law charge distribution, cluster emission rate, nuclear Zipf law, bimodality, the largest fluctuation of the fragments, Δ-scaling, caloric curve, phase coexis- tence diagram, critical temperature, critical exponent analysis, negative specific heat capacity and spinodal instability etc. The systematic works of the authors on experimental and theoretical LGPT are also introduced.

Digital RF phase detector for Linac in FEL accelerator

The digital RF (Radio Frequency) phase detector based on commercial PXI (PCI eXtensions for Instru- mentation) modules for the Linac is fully described in the paper. The DBM (Double Balance Mixer) is used as the phase detector and its control and data acquisition system is based on the PXI bus. The software adopts a curve fitting algorithm. The prototype has been tested in the laboratory and the good resolution, accuracy, reproducibility and re- liability are expected. The system does not present the problems of analog solution.

Characteristics of coherent diffraction terahertz radiation at SDUV-FEL

Coherent diffraction radiation (CDR), generated by relativistic femtosecond electron bunches passing through an aperture in a metallic foil, is becoming widely used as a high brightness, coherent, polarized, and broad- band terahertz radiation source. It is one of the most promising technique for non-destructive beam diagnostics in ac- celerators. In this paper, how to produce CDR is studied by utilizing femtosecond electron bunches at wavelengths equal to or longer than the bunch length with the aperture size at the condition of a≤γλ/(2π), which is provided by Shanghai Deep Ultraviolet-Free Electron Laser (SDUV-FEL) facility. General characteristics of CDR at SDUV-FEL are analyzed and numerically calculated. The results show that the radiance of this radiation within the frequency range from 0.2 up to 5 terahertz greatly exceeds that available from conventional black body radiation or synchrotron radiation.

Terahertz propagation in metal templates

Terahertz (THz) propagation in a metal template has been theoretically demonstrated. The possible propagating frequencies and power transmittance have been calculated. The power transmittance curves are displayed with different incident frequency and different geometric parameters. The oscillations of transmittance dependent on length of the template and the theoretical propagating bandwidth have been found.

Phase servo tuner control system of SSRF 500MHz cavity

Eight 500 MHz cavities are applied in the storage ring of the Shanghai Synchrotron Radiation Factility (SSRF). In order to tune the cavities for different changing parameters and operation modes, the control system is es- sential for operations. The tuning is achieved by synchronizing the drive signal and the cavity probe signal. The error signal defined as their subtraction is amplified and used to drive a stepping motor, which in turn moves a metallic plunger in or out of the cavity by programmable logic controller (PLC). The tune speed is set to be 1kHz/s.

Studies on aggregation-propensities and secondary structural transformations of proteins

The insoluble and fibrillar aggregates of some proteins are thought to be the pathological cause of neu- rodegenerative diseases. The aggregation-propensities of different types of proteins were investigated by Thioflavine T fluorescence assay and atomic force microscopy imaging. Then, the structural transformations of the proteins from aqueous state to solid state were studied by circular dichroism spectroscopy. The results indicate that proteins of dif- ferent secondary structure show variations in their aggregation-propensities, together with their various structural transformations from aqueous state to solid state. Our studies imply that the structural transformation of proteins from solution to solid state is closely associated with their aggregation-propensities, which will provide insight into the molecular mechanism of protein aggregation in neurodegenerative diseases.

The property of Si/SiGe/Si heterostructure during thermal budget characterized by HRXRD

Si/SiGe/Si heterostructures grown by ultra-high-vacuum chemical vapor deposition (UHVCVD) werecharacterized by Rutherford backscattering/Channeling (RBS/C) together with high resolution X ray diffraction(HRXRD). High quality SiGe base layer was obtained. The Si/SiGe/Si heterostructures were subject to conventionalfurnace annealing and rapid thermal annealing with temperature between 750 ℃ and 910 ℃. Both strain and its re-laxation degree in SiGe layer are calculated by HRXRD combined with elastic theory, which are never reported inother literatures. The rapid thermal annealing at elevated temperature between 880 ℃ and 910 ℃ for very short timehad almost no influence on the strain in Si0.84Ge0. 16 epilayer. However, high temperature (900℃) furnace annealingfor 1h prompted the strain in Si0.84Ge0.16 layer to relax.

Study of radiation-induced grafting of acrylic acid-sodium styrene sulfonate onto poly (tetrafluoroethylene-co-hexafluoropropylene) film

Poly(tetrafluoroethylene-co-hexafluoropropylene) (FEP) films were immersed in aqueous solution of acrylic acid (AA) and sodium styrene sulfonate (SSS), then irradiated by Co γ-rays at 25℃. The effects of reaction 60 time,absorbed dose, dose-rate, inhibitor and monomer concentration on the grafting yield were studied. Grafting yields of both AA and SSS onto FEP, respectively, increase with irradiation dose, but some saturation will appear at high dose and monomer concentration. The grafting yield increases with reaction time and then levels off. The graft- ing of SSS onto FEP is more difficult than the grafting of AA. The analysis of grafted membranes using DSC and FT-IR have been done.

Use of sampling based correction for non-radioactivity X-ray energy calibration

As the requirement of non-radioactivity measurement has increased in recent years,various energy cali- bration methods applied in portable X-ray fluorescence (XRF) spectrometers have been developed. In this paper,a sampling based correction energy calibration has been discussed. In this method both history information and current state of the instrument are considered and relative high precision and reliability can be obtained.

Pulse radiolysis study on aqueous solution of nicotine

Nicotine has been studied for the first time by pulse radiolysis techniques. It has been found that hy- drated electrons, hydrogen radicals and hydroxyl radicals can react with nicotine to produce anion radicals and neu- tral radicals, respectively, and the related rate constants have been determined.

Nano-manipulation of single DNA molecules

Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotech- nology. This review paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM). Precise manipulation has been realized including varied manipulating modes such as “cutting”, “pushing”, “folding”, “kneading”, “picking up”, “dipping”, etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.