A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signa...A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace leve1s; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.展开更多
A new dendrobine-type alkaloid has been isolated from stems of Dendrobium nobile lindl.. Its structure has been identified on the basis of spectroscopic method.
The present study was designed to determine the changes of phosphorylation of cAMP- response ele-ment binding protein (CREB) in hippocampus induced by ohmefentanyl stereoisomers (F9202 and F9204)in conditioned place p...The present study was designed to determine the changes of phosphorylation of cAMP- response ele-ment binding protein (CREB) in hippocampus induced by ohmefentanyl stereoisomers (F9202 and F9204)in conditioned place preference (CPP) paradigm. The results showed that mice receiving F9202 and F9204displayed obvious CPP. They could all significantly stimulate CREB phosphorylation and maintained for along time without affecting total CREB protein levels. The effect of F9204 was similar to morphine whicheffect was more potent and longer than F9202. We also examined the effects of ketamine, a noncompetitiveN-mthyl-D-aspartate receptor (NR) antagonist, on morphine-, F9202- and F9204- induced CPP and phos-phorylation of CREB in hippocampus. Ketamine could suppress not only the place preference but also thephosphorylation of CREB produced by morphine, F9202 and F9204. These findings suggest that alterationsin the phosphorylation of CREB be relevant to opiates signaling and the development of opiates dependence.NR antagonists may interfere with opiates dependence and may have potential therapeutic implications.展开更多
A new bibenzyl glycoside has been isolated from the stems of Dendrobium moniliforme (L.) Sw. (Orchidaceae). Its structure has been identified on the basis of spectroscopic and chemical methods.
Aim: To ascertain whether the side effects of gossypol, hypokalemia and irreversibility, could be avoided on dose re-duction. Methods: Seventy-seven male volunteers were divided into 3 groups: control (22 cases), 10 m...Aim: To ascertain whether the side effects of gossypol, hypokalemia and irreversibility, could be avoided on dose re-duction. Methods: Seventy-seven male volunteers were divided into 3 groups: control (22 cases), 10 mg gossypol(29 cases) and 12.5 mg (26 cases). Serum levels of testosterone, FSH and LH were measured by RIA and potassiumby flame photometry. Spema counts and motility were examined before and regularly after treatment for the evaluationof contraceptive efficacy. Results: The average sperm density and motility started to decrease significantly by theend of month 2 of medication and gradually reached the infertility levels ( < 4 million /mL) in both treated groups. Af-ter that the 10 mg group was asked to take the same dose every other day for up to a total observation period of 16-18months for the maintenance of infertility. Subjects in the 12.5 mg group did not take gossypol any more so as to ob-serve the length of the loading dose required, but in a few, a maintenance dose of 12.5 mg every other day was insti-tuted for a few more months. In both treated groups, none of the spouses was pregnant during the maintenance dose pe-riod. Serum levels of potassium, FSH, LH and testosterone were not significantly changed and not a single volunteercomplained of myoasthenia. After cessation of drug administratioin, the semen data returned to pretreatment levels.Conclusion: A regimen with 10 or 12.5 mg of gossypol as the daily loading dose and 35 or 43.75 mg as the week-ly maintenance dose could induce infertility in male volunteers without developing hypokalemia or irreversibility.(Asian J Androl 2000 Dec; 2: 283-287)展开更多
A new picrotoxane type sesquiterpene named dendrodensiflorol has been isolated from the stems of Dendrobium densiflorum. Its structure was identified on the basis of spectroscopic method.
A new approach for asymmetric synthesis of alkannin and shikonin is presented. The chiral centers of the targets were introduced via an asymmetric C-arylation of protected chiral glyceraldehyde in high de. The two e...A new approach for asymmetric synthesis of alkannin and shikonin is presented. The chiral centers of the targets were introduced via an asymmetric C-arylation of protected chiral glyceraldehyde in high de. The two enantiomers were prepared with the D-isopropylidenegly- ceraldehyde as the starting material.展开更多
A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s...A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s camphor sultam as chiral auxiliary.展开更多
Two new phenolic glycosides, everninic acid methyl ester-2-O-b-D- xylopyran- osyl-(16)-b-D-glucopyranoside 1 and 7-hydroxy-5-methoxyphthalide-7-b-D- xylopyranosyl- (16)-b-D-glucopyranoside 2 were isolated from the roo...Two new phenolic glycosides, everninic acid methyl ester-2-O-b-D- xylopyran- osyl-(16)-b-D-glucopyranoside 1 and 7-hydroxy-5-methoxyphthalide-7-b-D- xylopyranosyl- (16)-b-D-glucopyranoside 2 were isolated from the roots of Rhododendron molle G. Don. Their structures were determined by spectral evidence including 2D NMR techniques.展开更多
H3, a homogeneous acidic polysaccharide was obtained from the seeds of Cuscuta chinensisLam. Its structure was characterized for the first time by chemical and spectroscopic methods to be a highly branchedheteropolysa...H3, a homogeneous acidic polysaccharide was obtained from the seeds of Cuscuta chinensisLam. Its structure was characterized for the first time by chemical and spectroscopic methods to be a highly branchedheteropolysaccharide with mean molecular weight of more than lxl0^6. It was composed of 1,6-1inked-β-D Galp, 1,4-linked-β-D Galp, 1,4-1inked-β-D GalA, 1,3,6-1inked-β-D Galp and 1,2,4-1inked Rhap, with branching points at O-2or 0-4 of 1,2,4-1inked Rhap and 0-3 of 1,3,6-1inked-β-D Galp. Its side chains included 1-1inked Araf, 1,5-1inked Arafand 1,3,5-1inked Araf at O-3 of 1,6-1inked Galp in the main chain.展开更多
A new furostanoside, aspafilioside D (1) has been isolated from the root of Asparagus filicinus. Its structure was determined by spectral and chemical methods.
Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshow...Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshown to inhibit the growth of cultured rat luteal cells.It has been known that 2-phenylindole derivatives couldcompete with estradiol for the estrogen receptor. Theseresults prompted us to design and synthesize the follow-ing six new 3-oxamoyl-2-phenylindole compounds 1a-fand test their effects on the growth of luteal cells, aimingat seeking new lead compounds possessing antifertility activities.展开更多
A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the a...A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the anti-osteoporosis agents.展开更多
文摘A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace leve1s; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.
文摘A new dendrobine-type alkaloid has been isolated from stems of Dendrobium nobile lindl.. Its structure has been identified on the basis of spectroscopic method.
文摘The present study was designed to determine the changes of phosphorylation of cAMP- response ele-ment binding protein (CREB) in hippocampus induced by ohmefentanyl stereoisomers (F9202 and F9204)in conditioned place preference (CPP) paradigm. The results showed that mice receiving F9202 and F9204displayed obvious CPP. They could all significantly stimulate CREB phosphorylation and maintained for along time without affecting total CREB protein levels. The effect of F9204 was similar to morphine whicheffect was more potent and longer than F9202. We also examined the effects of ketamine, a noncompetitiveN-mthyl-D-aspartate receptor (NR) antagonist, on morphine-, F9202- and F9204- induced CPP and phos-phorylation of CREB in hippocampus. Ketamine could suppress not only the place preference but also thephosphorylation of CREB produced by morphine, F9202 and F9204. These findings suggest that alterationsin the phosphorylation of CREB be relevant to opiates signaling and the development of opiates dependence.NR antagonists may interfere with opiates dependence and may have potential therapeutic implications.
文摘A new bibenzyl glycoside has been isolated from the stems of Dendrobium moniliforme (L.) Sw. (Orchidaceae). Its structure has been identified on the basis of spectroscopic and chemical methods.
文摘Aim: To ascertain whether the side effects of gossypol, hypokalemia and irreversibility, could be avoided on dose re-duction. Methods: Seventy-seven male volunteers were divided into 3 groups: control (22 cases), 10 mg gossypol(29 cases) and 12.5 mg (26 cases). Serum levels of testosterone, FSH and LH were measured by RIA and potassiumby flame photometry. Spema counts and motility were examined before and regularly after treatment for the evaluationof contraceptive efficacy. Results: The average sperm density and motility started to decrease significantly by theend of month 2 of medication and gradually reached the infertility levels ( < 4 million /mL) in both treated groups. Af-ter that the 10 mg group was asked to take the same dose every other day for up to a total observation period of 16-18months for the maintenance of infertility. Subjects in the 12.5 mg group did not take gossypol any more so as to ob-serve the length of the loading dose required, but in a few, a maintenance dose of 12.5 mg every other day was insti-tuted for a few more months. In both treated groups, none of the spouses was pregnant during the maintenance dose pe-riod. Serum levels of potassium, FSH, LH and testosterone were not significantly changed and not a single volunteercomplained of myoasthenia. After cessation of drug administratioin, the semen data returned to pretreatment levels.Conclusion: A regimen with 10 or 12.5 mg of gossypol as the daily loading dose and 35 or 43.75 mg as the week-ly maintenance dose could induce infertility in male volunteers without developing hypokalemia or irreversibility.(Asian J Androl 2000 Dec; 2: 283-287)
文摘A new picrotoxane type sesquiterpene named dendrodensiflorol has been isolated from the stems of Dendrobium densiflorum. Its structure was identified on the basis of spectroscopic method.
基金the National Natural Science Foundation of China[32071532]for financial supports
文摘A new approach for asymmetric synthesis of alkannin and shikonin is presented. The chiral centers of the targets were introduced via an asymmetric C-arylation of protected chiral glyceraldehyde in high de. The two enantiomers were prepared with the D-isopropylidenegly- ceraldehyde as the starting material.
文摘A new method for preparation of the potent hypoglycemic KAD-1229 was developed. The key step of this method is diasteroselective alkylation in high optical purity and good yield by using easily available Oppolzer’s camphor sultam as chiral auxiliary.
基金This work is supported by National Natural Science Foundation of China (Grant No. 30170104).
文摘Two new phenolic glycosides, everninic acid methyl ester-2-O-b-D- xylopyran- osyl-(16)-b-D-glucopyranoside 1 and 7-hydroxy-5-methoxyphthalide-7-b-D- xylopyranosyl- (16)-b-D-glucopyranoside 2 were isolated from the roots of Rhododendron molle G. Don. Their structures were determined by spectral evidence including 2D NMR techniques.
文摘H3, a homogeneous acidic polysaccharide was obtained from the seeds of Cuscuta chinensisLam. Its structure was characterized for the first time by chemical and spectroscopic methods to be a highly branchedheteropolysaccharide with mean molecular weight of more than lxl0^6. It was composed of 1,6-1inked-β-D Galp, 1,4-linked-β-D Galp, 1,4-1inked-β-D GalA, 1,3,6-1inked-β-D Galp and 1,2,4-1inked Rhap, with branching points at O-2or 0-4 of 1,2,4-1inked Rhap and 0-3 of 1,3,6-1inked-β-D Galp. Its side chains included 1-1inked Araf, 1,5-1inked Arafand 1,3,5-1inked Araf at O-3 of 1,6-1inked Galp in the main chain.
基金This work was supported by the National Natural Science Fundation of China (30100229) and Science and Technology Development Fundation of Shanghai China (01QB14051) and these supports are gratefully acknowledged.
文摘A new furostanoside, aspafilioside D (1) has been isolated from the root of Asparagus filicinus. Its structure was determined by spectral and chemical methods.
文摘Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshown to inhibit the growth of cultured rat luteal cells.It has been known that 2-phenylindole derivatives couldcompete with estradiol for the estrogen receptor. Theseresults prompted us to design and synthesize the follow-ing six new 3-oxamoyl-2-phenylindole compounds 1a-fand test their effects on the growth of luteal cells, aimingat seeking new lead compounds possessing antifertility activities.
文摘A simple and efficient procedure for synthesis of 1-hydroxymethylene-1,1-bisphos- phoates from aldehydes is described. This method was applied to the synthesis of novel catechol substituted bisphosphonates as the anti-osteoporosis agents.