A sensitive, specific, noncompetitive, sandwich-type radioimmunoassayfor carcinoembryonic antigen (CEA) has been developed in our laboratoryl which canbe performed conveniently. The assay involves two monoclonal antib...A sensitive, specific, noncompetitive, sandwich-type radioimmunoassayfor carcinoembryonic antigen (CEA) has been developed in our laboratoryl which canbe performed conveniently. The assay involves two monoclonal antibodies, selectedfor high affinity and specificity and also for reaction against antigenic sites on CEAthat are distal from each other. One of these antibodies was labeled with 1251 andthe other was conjugated covalently to biotin. Polystyrene tubes were conjugatedcovalently to avidin. These tubes represent a rapid, simple method for separating theCEA-bound antibody from the free antibody. The biotin-antibody-CEA-"’I-labeledantibody complexes bind to the tubes and CEA concentration is directly related tocounts per minute. This assay can detect the CEA at a concentration of 0.22 ig/L inserum.展开更多
A nuclear microprobe with high spatial resolution and high analytical sensitivity was applied to analyze trace elements, especially lead, in vehicle exhaust of Shanghai city. The result shows that the chemical composi...A nuclear microprobe with high spatial resolution and high analytical sensitivity was applied to analyze trace elements, especially lead, in vehicle exhaust of Shanghai city. The result shows that the chemical composition and its corresponding x-ray relative intensity are different among different vehicle exhausts. There are many kinds of metal elements in particles of vehicle exhaust, most arc harmful to people, such as Ti, Cr, Mn, Ph. etc. We found that the lead concentration was 6820 μg/g and the bromine concentration was 5300 μg/g iii the exhaust from Santana using leaded gasoline (SULG), which is higher than any other kinds of vehicle exhausts. We have also detected the minimum lead in the particles of unleaded gasoline and its content varies from one to another. Its mean concentration was 450 μg/g and the highest reached 6210 μg/g. The unleaded gasoline’s Pb existed in the whole particle while the leaded gasoline’s enriched in the surface of the particle and was more harmful to the human beings.展开更多
Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was...Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was used as reduction agent of antibody and the reduction of 186 ReO4^- was finished in sodium gluconate solution(pH=2.54),the labeling efficiency was 96.2%,After purification,the labeled products were tested in respect of immunoreactivity,in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice,188ReO4^- labeled colloid was synthesized by using a two-step method.The size of radiocolloid particles was around 2-5μm,and the radiochemical yield was over 95%,Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-collied and ^188 Re-McAb-SZ39 was 90% and 65% ,respectively.展开更多
文摘A sensitive, specific, noncompetitive, sandwich-type radioimmunoassayfor carcinoembryonic antigen (CEA) has been developed in our laboratoryl which canbe performed conveniently. The assay involves two monoclonal antibodies, selectedfor high affinity and specificity and also for reaction against antigenic sites on CEAthat are distal from each other. One of these antibodies was labeled with 1251 andthe other was conjugated covalently to biotin. Polystyrene tubes were conjugatedcovalently to avidin. These tubes represent a rapid, simple method for separating theCEA-bound antibody from the free antibody. The biotin-antibody-CEA-"’I-labeledantibody complexes bind to the tubes and CEA concentration is directly related tocounts per minute. This assay can detect the CEA at a concentration of 0.22 ig/L inserum.
基金Supported by the National Natural science Foundation of China (Major Project No. 19392100, andProject No. 19475061)
文摘A nuclear microprobe with high spatial resolution and high analytical sensitivity was applied to analyze trace elements, especially lead, in vehicle exhaust of Shanghai city. The result shows that the chemical composition and its corresponding x-ray relative intensity are different among different vehicle exhausts. There are many kinds of metal elements in particles of vehicle exhaust, most arc harmful to people, such as Ti, Cr, Mn, Ph. etc. We found that the lead concentration was 6820 μg/g and the bromine concentration was 5300 μg/g iii the exhaust from Santana using leaded gasoline (SULG), which is higher than any other kinds of vehicle exhausts. We have also detected the minimum lead in the particles of unleaded gasoline and its content varies from one to another. Its mean concentration was 450 μg/g and the highest reached 6210 μg/g. The unleaded gasoline’s Pb existed in the whole particle while the leaded gasoline’s enriched in the surface of the particle and was more harmful to the human beings.
文摘Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was used as reduction agent of antibody and the reduction of 186 ReO4^- was finished in sodium gluconate solution(pH=2.54),the labeling efficiency was 96.2%,After purification,the labeled products were tested in respect of immunoreactivity,in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice,188ReO4^- labeled colloid was synthesized by using a two-step method.The size of radiocolloid particles was around 2-5μm,and the radiochemical yield was over 95%,Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-collied and ^188 Re-McAb-SZ39 was 90% and 65% ,respectively.
