Objective: To investigate further into the osteogenic potential of rabbit dermal fibroblasts with scanning electron microscope. Methods: Split thickness rabbit skin was processed into small chips that were cultured in...Objective: To investigate further into the osteogenic potential of rabbit dermal fibroblasts with scanning electron microscope. Methods: Split thickness rabbit skin was processed into small chips that were cultured in vitro and then subjected to scanning electron microscopic study. Results: The fibroblasts swam out from the skin chips and they increased in number rapidly and became confluent. The cells exhibited squamous configuration, possessing arboreal bifurcation and forming multi layer structure. The fibroblasts then excreted numerous minute granules, heaping up on and around the cells. Thenceforth emerged on the cell surface fine needle like crystals, that agglomerated with the granules to form nodules. The fibroblasts orientated themselves in a radiating pattern around the large nodules. Neighboring nodules could be linked up into trabecular structures. Conclusions: Sequence of events of new bone formation by rabbit dermal fibroblasts cultured in vitro is fully depicted and confirmed.展开更多
Objective To investigate ultrastructurallyinto traumatic avascular necrosis of femoralhead. Methods Femoral heads were procuredduring endoprosthetic replacement for 8 casesof intracapsular fracture of femoral neck,whi...Objective To investigate ultrastructurallyinto traumatic avascular necrosis of femoralhead. Methods Femoral heads were procuredduring endoprosthetic replacement for 8 casesof intracapsular fracture of femoral neck,which had been subjected to internal fixationand developed Stage Ⅱ avascular necrosis.The specimens were processed and展开更多
文摘Objective: To investigate further into the osteogenic potential of rabbit dermal fibroblasts with scanning electron microscope. Methods: Split thickness rabbit skin was processed into small chips that were cultured in vitro and then subjected to scanning electron microscopic study. Results: The fibroblasts swam out from the skin chips and they increased in number rapidly and became confluent. The cells exhibited squamous configuration, possessing arboreal bifurcation and forming multi layer structure. The fibroblasts then excreted numerous minute granules, heaping up on and around the cells. Thenceforth emerged on the cell surface fine needle like crystals, that agglomerated with the granules to form nodules. The fibroblasts orientated themselves in a radiating pattern around the large nodules. Neighboring nodules could be linked up into trabecular structures. Conclusions: Sequence of events of new bone formation by rabbit dermal fibroblasts cultured in vitro is fully depicted and confirmed.
基金Shanghai No.2 Medical University,Shanghai 200025,China (Tang XM).
文摘Objective To investigate ultrastructurallyinto traumatic avascular necrosis of femoralhead. Methods Femoral heads were procuredduring endoprosthetic replacement for 8 casesof intracapsular fracture of femoral neck,which had been subjected to internal fixationand developed Stage Ⅱ avascular necrosis.The specimens were processed and
