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NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor 被引量:12
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作者 HUXiangyang S.J.Neill 《Chinese Science Bulletin》 SCIE EI CAS 2003年第4期358-363,共6页
Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice Xiushui? The elicitor from the cell wall of M. grisea race 98-186-1G1, termed IE, strongly ... Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice Xiushui? The elicitor from the cell wall of M. grisea race 98-186-1G1, termed IE, strongly in-duced the HR response in suspension cultures of rice Xiushui? including increased PAL activity, transcription of pal , pr1, chi, cell death and the generation of NO. The elici-tor prepared from the cell wall of M. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in Xiushui?rice. The increased PAL activity and transcription of pr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription of pr1, pal and chi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induc-tion of cell death by IE in rice suspension cells. 展开更多
关键词 水稻 悬浮培养 植物细胞 不相容性病原 超敏反应 NO 一氧化氮 介导
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Kinetics of the Phenotype and Function of Murine Peritoneal Macrophages Following Acute Inflammation 被引量:1
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作者 QingliWu YonghongFeng +6 位作者 YifuYang Jingliu WenliangZhou PeilanHe RuZhou XiaoyuLi JianpingZou 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2004年第1期57-62,共6页
This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages.A comprehensive kinetics of cytokine productio... This study was undertaken to have a better understand for the process and the underlying mechanisms to limit macrophage activation and population of activated macrophages.A comprehensive kinetics of cytokine production was performed in murine peritoneal macrophages recovered from Balb/c mice at various time during the course of an intraperitoneal injection with thioglycollate (TG).The expression of cell surface molecules such as MHC-Ⅰ,MHC-Ⅱ,B7-1 and B7-2 of these macrophages were also determined by flow cytometry.The present findings of our research suggested that the population of activated macrophages and the activation of macrophages (including cytokines production and expression of cell surface functional molecules) were strictly controlled during inflammation process.This is one of the important mechanisms to retain the host homeostasis.Cellular & Molecular Immunology.2004;1(1):57-62. 展开更多
关键词 MACROPHAGE CYTOKINE PHENOTYPE SELF-REGULATION
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