Changes of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment and their clinical value

BACKGROUND Osteoarthritis is a chronic degenerative disease with an incidence of 50%in people over 65 years old and 80%in people over 80 years old worldwide.It is the second leading reason of loss of working capacity after cardiovascular diseases and severely affects the society and families.Therefore,finding biological markers related to the diagnosis and treatment of osteoarthritis is of great significance in clinical practice.AIM To observe the changes and clinical value of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment.METHODS Eighty-three patients with knee osteoarthritis(observation group)who were admitted to our hospital from April 2013 to June 2015,and 60 healthy people(control group)during the same period were selected.After 4 wk of treatment,the levels of miR-145,tumor necrosis factor(TNF)-α,interleukin(IL)-6,and IL-10 were compared between the control group and the observation group before treatment.The correlation of miR-145,TNF-α,IL-6,and IL-10 levels with visual analogue scale(VAS),Lysholm,and Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)scores was assessed by Pearson correlation analysis.The correlation of the expression of miR-145,TNF-α,IL-6,and IL-10 with Kellgren-Lawrence(K-L)grades was assessed by Spearman correlation analysis.The critical levels of miR-145,TNF-α,IL-6,and IL-10 in distinguishing different K-L grades were determined by receiver operating characteristic(ROC)curve analysis.RESULTS The expression level of miR-145 in the observation group was significantly higher than that in the control group before treatment(P<0.05).After treatment,the expression level of miR-145 in the observation group was significantly lower than that before treatment(P<0.05).The levels of TNF-αand IL-6 in the observation group were significantly higher than those in the control group(P<0.05),and the level of IL-10 was significantly lower than that in the control group(P<0.05).After treatment,the levels of TNF-αand IL-6 in the observation group were significantly lower than those before treatment(P<0.05),and IL-I0 level was significantly higher than that before treatment(P<0.05).VAS and WOMAC scores were both positively correlated with miR-145,TNF-α,and IL-6(P<0.05),and negatively correlated with IL-10(P<0.05),while Lysholm scores were negatively correlated with miR-145,TNF-α,and IL-6(P<0.05),and positively correlated with IL-10(P<0.05).K-L grades were positively correlated with miR-145,TNF-α,and IL-6(P<0.05),and negatively correlated with IL-10(P<0.05).The area under the ROC curve(AUC)and specificity of TNF-αin differentiating K-L grades I-II were the highest,which were 0.785 and 97.45%,respectively,and miR145 had the highest sensitivity of 94.59%;the AUC and sensitivity of IL-6 in differentiating K-L grades II-III were the highest,which were 0.766 and 97.30%,respectively,and TNF-αhad the highest specificity of 86.68%.CONCLUSION MiR-145 and inflammatory factors have certain diagnostic value in osteoarthritis,and they are expected to become potential indicators for the diagnosis and evaluation of osteoarthritis in the future.

Safety and Efficacy of Frameless Stereotactic Brain Biopsy Techniques

Objective To explore the safety and efficacy of frameless stereotactic brain biopsy.Methods Diagnostic accuracy was calculated by comparing biopsy diagnosis with definitive pathology in 62 patients who underwent frameless stereotactic brain biopsy between January 2008 and December 2010 in Xiamen University Southeast Hospital.Preoperative characteristics and histological diagnosis were reviewed and then information was analysed to identify factors associated with the biopsy not yielding a diagnosis and complications.Results Diagnostic yield was 93.5%.No differences were found between pathological diagnosis and frozen pathological diagnosis.The most common lesions were astrocytic lesions,included 16 cases of low-grade glioma and 12 cases of malignant glioma.Remote hemorrhage,metastasis,and lymphoma were following in incidence.Multiple brain lesions were found in 17 cases (27.4%).Eleven cases were frontal lesions (17.7%),8 were frontotemporal (12.9%),6 were frontoparietal (9.7%),and 5 each were temporal,parietal,and parietotemporal lesions (8.1%).Postoperative complications occurred in 21.0% of the patients after biopsies,including 10 haemorrhages (16.1%) and 3 temporary neurological deficits (1 epilepsy,1 headache,and 1 partial hemiparesis).No patient required operation for hematoma evacuation.Conclusion Frameless stereotactic biopsy is an effective and safe technique for histologic diagnosis of brain lesions,particularly for multifocal and frontal lesions.

Treatment of Lacunar Cerebral Infarction with Huo Xue Tong Luo Tang

Based on our clinical experience,we formulated Huo Xue Tong Luo Tang(活血通络汤HXTL Decoction),a prescription for treatment of lacunar cerebral infarction.Most of the ingredients are those in the prescriptions of Di Dang Tang(抵当汤)and Gui Gan Long Mu Tang(桂甘龙牡汤)described in the book Treatise of Febrile Diseases(伤寒论)in accordance with the compatible theory of principal,assistant,adjuvant and guiding drugs for improving blood circulation,removing stasis,dispelling endogenous wind and phlegm,inducing resuscitation,removing obstruction in the channels,and balancing the yin and yang.

T-bet, GATA-3 and Foxm1 expression in chronic sinusitis mucosa and their correlation with inflammatory molecule expression

Objective: To study the T-bet, GATA-3 and Foxm1 expression in chronic sinusitis mucosa and their correlation with inflammatory molecule expression. Methods: Patients with sinusitis and deflection of nasal septum who received nasal endoscopic surgery in Shengli hospital between June 2015 and February 2017 were selected and enrolled in CRS group and control group respectively. The nasal mucosa tissue was collected during operation to determine the mRNA expression of T-bet, GATA-3 and Foxm1 as well as the protein expression of inflammatory molecules. Results: T-bet, GATA-3 and Foxm1 mRNA expression as well as IL-1β, IFN-γ, TNF-α, IL-4, IL-5, IL-19, IL-20R1, IL-20R2, MMP2, MMP9, PI3K, Akt, GSK-3β and p38MAPK protein expression in nasal mucosa tissue of CRS group were significantly higher than those of control group;T-bet, GATA-3 and Foxm1 mRNA expression in nasal mucosa tissue of patients with CRS were positively correlated with IL-1β, IFN-γ, TNF-α, IL-4, IL-5, IL-19, IL-20R1, IL-20R2, MMP2, MMP9, PI3K, Akt, GSK-3β and p38MAPK protein expression. Conclusions: The high expression of T-bet, GATA-3 and Foxm1 in chronic sinusitis mucosa tissue can activate Th1 and Th2 immune response and cause mucosal inflammatory response.

Behavioral problems and related factors in children of different aged patients with schizophrenia: A cross-sectional study

BACKGROUND： With the age growing, changes of behavioral problems in children whose parents had schizophrenia, differences of related factors and ways for particular intervention should be further studied. OBJECTIVE： To survey the behavioral problems in children of different aged patients with chizophrenia and investigate the correlation between behavioral problems and related factors in different aged groups. DESIGN： Cross-sectional study. SETTING： Shandong Mental Health Center. PARTICIPANTS： A total of 364 patients with schizophrenia were selected from eleven psychiatric hospitals from June 1999 to June 2000. There were 179 males and 185 females, and their ages ranged from 28 to 45 years. All patients met modified diagnostic criteria of schizophrenia （the second edition）, China Classification of Mental Diseases and Diagnostic Criteria. Meanwhile, children of the 364 patients were grouped based on their ages, including 6 - 1 l years old group [n -217; 114 males and 103 females; mean age of （9±2） years] and 12 - 16 years old group [n = 147; 99 males and 48 females; mean age of （14±1） years]. The Chinese norms of Achenbach＇s Child Behavior Check List were regarded as the normal control group. METHODS： Children who received self-made mental health related factors inventory and Achenbach＇s Child Behavior Check List were involved in this study. All children and their parents provided the confirmed consent. Achenbach＇s Child Behavior Check List was used for parents to mainly evaluate children, and the results manifested various behavioral problems based on different sexes and different ages. Self-made mental health related factors inventory contained questionnaires for parents and children, respectively. In this study, home situation of parents as well as personality characteristics and educational styles of children were mainly evaluated. MAIN OUTCOME MEASURES： ① Scores of Achenbach＇s Child Behavior Check List in different aged children; ② Scores of self-made mental health related factors inventory based on different home situations and educational styles. RESULTS： Children of 364 patients were involved in the final analysis. ① There were no significant differences in total score of Achenbach＇s Child Behavior Check List and sex in the two groups （P 〉 0.05）. There were significant differences in schizoid, body recount, aggression, depression, hyperactivity, violating discipline, compulsion and total abnormal rate of boys between the 6 - 11 years old group and the normal control group （P 〈 0.05 - 0.01）. In addition, there were also significant differences in poor communication, depression, hyperactivity, violating discipline, compulsion and total abnormal rate of girls between the 6 - 11 years old group and the normal control group （P 〈 0.05 - 0.01）. ② As compared with normal control group,scores of home environment in the two aged groups demonstrated that educational level was low; interpersonal relationship was poor; relation to children was not closed; relationship between husband and wife was not harmonious; positive rate of family history was high; economic status was poor; family atmosphere was poor. Therefore, there was significant difference （P 〈 0.01）. Educational level in parental generation and single parent family in the 6 - 11 years old group were higher than that in the 12 - 16 years old group, with significant difference （P 〈 0.05）. As compared with the normal control group, results of education demonstrated that children in the two aged groups had poor communication with other children; they had poor behaviors in schools; most of them were boarded and had poor relationship with their parents; teaching styles for children were different. CONCLUSION： All children of different aged patients with schizophrenia had poor home environment and education; and they were often affected by other factors. Behavioral problems were changed with the age growing; in addition, these changes were related to home environment, education and society. Therefore, comprehensive intervention may be an ideal method.