Objective To establish a rapid method for detecting acetylbritannilactone （ABL） by online sweeping-micellar electrokinetic chromatography （MEKC） and to elevate the sensitivity of the detection. Methods The combina...Objective To establish a rapid method for detecting acetylbritannilactone （ABL） by online sweeping-micellar electrokinetic chromatography （MEKC） and to elevate the sensitivity of the detection. Methods The combination of online sweeping technique with MEKC was used to determine the content of ABL in the extract of Inula britannica in plasma of rats. Results ABL was completely separated within 15 min in running buffer and sample buffer. The optimal conditions were as follows： on uncoated fused quartz silica capillary, with separation voltage of 23 kV, capillary temperature of 25 oC, and detection wavelength of 195 nm. The regression equations revealed good linear relationships between the peak area and concentration of ABL （r = 0.998）, with the detection limits of 0.005-0.15 mg/mL. The relative standard deviations of migration time and peak areas for intra- and inter-batch were 〈 2.45% and 〈 2.26%, respectively. The recovery rate of this method was 96.3~97.2%. Conclusion This method provides some advantages in separation speed, testing sensitivity, and operating convenience, with low sample and reagent consumption. The online sweeping-MEKC is an effective method for pharmacokinetic study and analysis on tracing biological samples.展开更多
The usefulness of biopartitioning micellar chromatography （BMC） for predicting oral drug acute toxicity and apparent bioavailability was demonstrated. A logarithmic model （an LD50 model） and the second order polyn...The usefulness of biopartitioning micellar chromatography （BMC） for predicting oral drug acute toxicity and apparent bioavailability was demonstrated. A logarithmic model （an LD50 model） and the second order polynomial models （apparent bioavailability model） have been obtained using the retention data of the selective calcium channel blockers to predict pharmacological properties of compounds. The use of BMC is simple, reproducible and can provide key information about the acute toxicity and transport properties of new compounds during the drug discovery process.展开更多
基金Hebei Province Supporting Natural Science Fund(No.06276470)
文摘Objective To establish a rapid method for detecting acetylbritannilactone （ABL） by online sweeping-micellar electrokinetic chromatography （MEKC） and to elevate the sensitivity of the detection. Methods The combination of online sweeping technique with MEKC was used to determine the content of ABL in the extract of Inula britannica in plasma of rats. Results ABL was completely separated within 15 min in running buffer and sample buffer. The optimal conditions were as follows： on uncoated fused quartz silica capillary, with separation voltage of 23 kV, capillary temperature of 25 oC, and detection wavelength of 195 nm. The regression equations revealed good linear relationships between the peak area and concentration of ABL （r = 0.998）, with the detection limits of 0.005-0.15 mg/mL. The relative standard deviations of migration time and peak areas for intra- and inter-batch were 〈 2.45% and 〈 2.26%, respectively. The recovery rate of this method was 96.3~97.2%. Conclusion This method provides some advantages in separation speed, testing sensitivity, and operating convenience, with low sample and reagent consumption. The online sweeping-MEKC is an effective method for pharmacokinetic study and analysis on tracing biological samples.
基金Project supported by the National Natural Science Foundation of China （No. 20375010）, Natural Science Foundation of Hebei Province （No. 202096）, Bairen Project of Chinese Academy of Sciences, and the Excellent Teacher Program and the Specialized Research Fund for the Doctoral Program of Higher Education.Acknowledgements The authors wish to thank professor Yang LIANG and Yongjian ZHANG for a generous gift of m-nisoldipine, nisoldipine and nicardipine.
文摘The usefulness of biopartitioning micellar chromatography （BMC） for predicting oral drug acute toxicity and apparent bioavailability was demonstrated. A logarithmic model （an LD50 model） and the second order polynomial models （apparent bioavailability model） have been obtained using the retention data of the selective calcium channel blockers to predict pharmacological properties of compounds. The use of BMC is simple, reproducible and can provide key information about the acute toxicity and transport properties of new compounds during the drug discovery process.
