Fifty-five representative samples of Rhizoctonia solani isolates, which were collected and isolated from five different ecological regions in Sichuan Province, China, were purified and analyzed for the pathogenicity a...Fifty-five representative samples of Rhizoctonia solani isolates, which were collected and isolated from five different ecological regions in Sichuan Province, China, were purified and analyzed for the pathogenicity and molecular genetic variation. The hyphal fusion test revealed that almost all the isolates belonged to the AG-IIA group except the isolate D42. In addition, some of the isolates were ＇bridging isolates＇, which could fuse with several groups simultaneously. The pathogenicity analysis on in vitro leaves confirmed a significant pathogenicity variation in the tested isolates. The 55 isolates were then classified into 8 groups by further RAPD （randomly amplified polymorphic DNA） cluster analysis at the similarity coefficient of 0.941. The results suggest that under the certain ecological conditions in Sichuan Province, China, most of the R. solanistrains were genetically stable, but a few changed drastically.展开更多
基金the National High- tech Research and Development Program of China (Grant No. 2003AA212030)the Foundation for Doctorial Research in the Southwest University of Science and Technology (Grant No. 07ZX0101)the Program for Changjiang Scholars and Innovative Research Team in University of China (PCSIRT)(Grant No. IRT0453)
文摘Fifty-five representative samples of Rhizoctonia solani isolates, which were collected and isolated from five different ecological regions in Sichuan Province, China, were purified and analyzed for the pathogenicity and molecular genetic variation. The hyphal fusion test revealed that almost all the isolates belonged to the AG-IIA group except the isolate D42. In addition, some of the isolates were ＇bridging isolates＇, which could fuse with several groups simultaneously. The pathogenicity analysis on in vitro leaves confirmed a significant pathogenicity variation in the tested isolates. The 55 isolates were then classified into 8 groups by further RAPD （randomly amplified polymorphic DNA） cluster analysis at the similarity coefficient of 0.941. The results suggest that under the certain ecological conditions in Sichuan Province, China, most of the R. solanistrains were genetically stable, but a few changed drastically.
