Rutin,a flavonoid found in fruits and vegetables,is a potential anticancer compound with strong anticancer activity.Therefore,electrochemical sensor was developed for the detection of rutin.In this study,CoWO_(4) nano...Rutin,a flavonoid found in fruits and vegetables,is a potential anticancer compound with strong anticancer activity.Therefore,electrochemical sensor was developed for the detection of rutin.In this study,CoWO_(4) nanosheets were synthesized via a hydrothermal method,and porous carbon(PC)was prepared via high-temperature pyrolysis.Successful preparation of the materials was confirmed,and characterization was performed by transmission electron microscopy,scanning electron microscopy,and X-ray photoelectron spectroscopy.A mixture of PC and CoWO_(4) nanosheets was used as an electrode modifier to fabricate the electrochemical sensor for the electrochemical determination of rutin.The 3D CoWO_(4) nanosheets exhibited high electrocatalytic activity and good stability.PC has a high surface-to-volume ratio and superior conductivity.Moreover,the hydrophobicity of PC allows large amounts of rutin to be adsorbed,thereby increasing the concentration of rutin at the electrode surface.Owing to the synergistic effect of the 3D CoWO_(4) nanosheets and PC,the developed electrochemical sensor was employed to quantitively determine rutin with high stability and sensitivity.The sensor showed a good linear range(5-5000 ng/mL)with a detection limit of 0.45 ng/mL.The developed sensor was successfully applied to the determination of rutin in crushed tablets and human serum samples.展开更多
The study of lipid metabolism relies on the characterization of the lipidome,which is quite complex due to the structure variations of the lipid species.New analytical tools have been developed recently for characteri...The study of lipid metabolism relies on the characterization of the lipidome,which is quite complex due to the structure variations of the lipid species.New analytical tools have been developed recently for characterizing fine structures of lipids,with C=C location identification as one of the major improvements.In this study,we studied the lipid metabolism reprograming by analyzing glycerol phospholipid compositions in breast cancer cell lines with structural specification extended to the C=C location level.Inhibition of the lipid desaturase,stearoyl-CoA desaturase 1,increased the proportion of n-10 isomers that are produced via an alternative fatty acid desaturase 2 pathway.However,there were different variations of the ratio of n-9/n-7 isomers in C18:1-containing glycerol phospholipids after stearoyl-CoA desaturase 1 inhibition,showing increased tendency in MCF-7 cells,MDA-MB-468 cells,and BT-474 cells,but decreased tendency in MDA-MB-231 cells.No consistent change of the ratio of n-9/n-7 isomers was observed in SK-BR-3 cells.This type of heterogeneity in reprogrammed lipid metabolism can be rationalized by considering both lipid desaturation and fatty acid oxidation,highlighting the critical roles of comprehensive lipid analysis in both fundamental and biomedical applications.展开更多
Gut microbiota plays an important role in coronary heart disease, but its compositional and functional changes in unstable angina(UA) remain unexplored. We performed metagenomic sequencing of 133 newly diagnosed UA pa...Gut microbiota plays an important role in coronary heart disease, but its compositional and functional changes in unstable angina(UA) remain unexplored. We performed metagenomic sequencing of 133 newly diagnosed UA patients and 133 sex-and age-matched controls, and profiled the fecal and plasma metabolomes in 30 case-control pairs. The alpha diversity of gut microbiota was increased in UA patients:the adjusted odds ratios(ORs) per standard deviation increase in Shannon and Simpson indices were 1.30(95% confidence interval, 1.01-1.70) and 1.36(1.05-1.81), respectively. Two common species(depleted Klebsiella pneumoniae and enriched Streptococcus parasanguinis;P ≤ 0.002) and three rare species(depleted Weissella confusa, enriched Granulicatella adiacens and Erysipelotrichaceae bacterium 6_1_45;P ≤ 0.005) were associated with UA. The UA-associated gut microbiota was depleted in the pathway of Lphenylalanine degradation(P = 0.001), primarily contributed by Klebsiella pneumoniae. Consistently, we found increased circulating phenylalanine in UA patients(OR = 2.76 [1.17-8.16]). Moreover, Streptococcus parasanguinis was negatively correlated with fecal citrulline(Spearman's r_(s)=-0.470, P = 0.009), a metabolite depleted in UA patients(OR = 0.26 [0.08-0.63]). These findings are informative to help understand the metabolic connection between gut microbiota and UA.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.:81872509)the Baoan TCM Development Foundation(Grant No.:2020KJCX-KTYJ-200)+7 种基金Internal Research Project of the Shenzhen Baoan Authentic TCM Therapy Hospital(Grant Nos.:BCZY2021003 and BCZY2021007)Baoan District Medical and Health Basic Research Project(Grant No.:2020JD491)Natural Science Foundation of Hubei Province(Grant No.:2019CFB429)Chinese Medicine Research Fund of Health Commission of Hubei Province(Grant Nos.:ZY2021M038 and ZY2021M051),the Youth Talent Project of Sinopharm Dongfeng General Hospital(Grant No.:2021Q03)the Science and Technology Key Program of Shiyan(Grant No.:21Y77)Baoan District Medical and Health Basic Research Project(Grant Nos.:2021JD143,2021JD281,and 2021JD290)Hubei Province Health and Family Planning Scientific Research Project(Grant Nos.:WJ2021M063 and WJ2021M062)Sanming Project of Medicine in Shenzhen(Grant No.:SZZYSM202106004).
文摘Rutin,a flavonoid found in fruits and vegetables,is a potential anticancer compound with strong anticancer activity.Therefore,electrochemical sensor was developed for the detection of rutin.In this study,CoWO_(4) nanosheets were synthesized via a hydrothermal method,and porous carbon(PC)was prepared via high-temperature pyrolysis.Successful preparation of the materials was confirmed,and characterization was performed by transmission electron microscopy,scanning electron microscopy,and X-ray photoelectron spectroscopy.A mixture of PC and CoWO_(4) nanosheets was used as an electrode modifier to fabricate the electrochemical sensor for the electrochemical determination of rutin.The 3D CoWO_(4) nanosheets exhibited high electrocatalytic activity and good stability.PC has a high surface-to-volume ratio and superior conductivity.Moreover,the hydrophobicity of PC allows large amounts of rutin to be adsorbed,thereby increasing the concentration of rutin at the electrode surface.Owing to the synergistic effect of the 3D CoWO_(4) nanosheets and PC,the developed electrochemical sensor was employed to quantitively determine rutin with high stability and sensitivity.The sensor showed a good linear range(5-5000 ng/mL)with a detection limit of 0.45 ng/mL.The developed sensor was successfully applied to the determination of rutin in crushed tablets and human serum samples.
基金This research is supported by the National Natural Science Foundation of China(Projects 21934003 and 21974077)the Natural Science Foundation of Hubei Provincial Department of Education(2019CFB429).
文摘The study of lipid metabolism relies on the characterization of the lipidome,which is quite complex due to the structure variations of the lipid species.New analytical tools have been developed recently for characterizing fine structures of lipids,with C=C location identification as one of the major improvements.In this study,we studied the lipid metabolism reprograming by analyzing glycerol phospholipid compositions in breast cancer cell lines with structural specification extended to the C=C location level.Inhibition of the lipid desaturase,stearoyl-CoA desaturase 1,increased the proportion of n-10 isomers that are produced via an alternative fatty acid desaturase 2 pathway.However,there were different variations of the ratio of n-9/n-7 isomers in C18:1-containing glycerol phospholipids after stearoyl-CoA desaturase 1 inhibition,showing increased tendency in MCF-7 cells,MDA-MB-468 cells,and BT-474 cells,but decreased tendency in MDA-MB-231 cells.No consistent change of the ratio of n-9/n-7 isomers was observed in SK-BR-3 cells.This type of heterogeneity in reprogrammed lipid metabolism can be rationalized by considering both lipid desaturation and fatty acid oxidation,highlighting the critical roles of comprehensive lipid analysis in both fundamental and biomedical applications.
基金supported by grants from Foundation of the National Key Research and Development Program of China (2016YFC0900800)the National Natural Science Foundation of China (81930092)+2 种基金the Fundamental Research Funds for the Central University (2019kfy XMBZ015)the 111 Projectthe Program for Changjiang Scholars and Innovative Research Team in University。
文摘Gut microbiota plays an important role in coronary heart disease, but its compositional and functional changes in unstable angina(UA) remain unexplored. We performed metagenomic sequencing of 133 newly diagnosed UA patients and 133 sex-and age-matched controls, and profiled the fecal and plasma metabolomes in 30 case-control pairs. The alpha diversity of gut microbiota was increased in UA patients:the adjusted odds ratios(ORs) per standard deviation increase in Shannon and Simpson indices were 1.30(95% confidence interval, 1.01-1.70) and 1.36(1.05-1.81), respectively. Two common species(depleted Klebsiella pneumoniae and enriched Streptococcus parasanguinis;P ≤ 0.002) and three rare species(depleted Weissella confusa, enriched Granulicatella adiacens and Erysipelotrichaceae bacterium 6_1_45;P ≤ 0.005) were associated with UA. The UA-associated gut microbiota was depleted in the pathway of Lphenylalanine degradation(P = 0.001), primarily contributed by Klebsiella pneumoniae. Consistently, we found increased circulating phenylalanine in UA patients(OR = 2.76 [1.17-8.16]). Moreover, Streptococcus parasanguinis was negatively correlated with fecal citrulline(Spearman's r_(s)=-0.470, P = 0.009), a metabolite depleted in UA patients(OR = 0.26 [0.08-0.63]). These findings are informative to help understand the metabolic connection between gut microbiota and UA.