We establish a system to measure the functional absorption cross section of photosystem lI （PSII） （O-PSII） and maximum quantum yield of photochemistry in PSII （Fv/Fm）. The system utilizes a sequence of high-freq...We establish a system to measure the functional absorption cross section of photosystem lI （PSII） （O-PSII） and maximum quantum yield of photochemistry in PSII （Fv/Fm）. The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters o-Psii and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation （RSD） of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.展开更多
Using a measurement system based on fluorescence induced by variable pulse light, photosynthesis parameters of chlorella pyrenoidosa are obtained, employing single-turnover and multiple-turnover protocols under darkad...Using a measurement system based on fluorescence induced by variable pulse light, photosynthesis parameters of chlorella pyrenoidosa are obtained, employing single-turnover and multiple-turnover protocols under darkadapted and light-adapted conditions. Under the light-adapted condition,σ’PSII is larger, and F’v/F’m（ST） and F’v/F’m（MT） are smaller than those of the dark-adapted condition, but the corresponding parameters possess good linear correlations.Fm（MT）, F’m（MT）, Fv/Fm（MT） and F’v/F’m（MT） which are measured using the multipleturnover protocol, are larger than those of the single-turnover protocol. The linear correlation coefficient between Fm（ST） and Fm（MT） is 0.984,and Fv/Fm（MT） = 1.18 Fv/Fm（ST） The linear correlation coefficient between F’m（ST） and F’m（MT） is 0.995, and F’v/F’m（MT） = 1.36 F’v/F’m/（ST）.展开更多
基金the Natural Science Foundation of Anhui Province(No.1408085MD72)the National"863"Program of China(Nos.2014AA06A509,2013AA065502,and 2009AA063005)+2 种基金the Science and Technology Planning Project of Anhui Province(No.1206c0805012)the National Natural Science Foundationof China(No.61378041)the Excellent Youth Foundation of Anhui Scientific Committee(No.1108085J19).
文摘We establish a system to measure the functional absorption cross section of photosystem lI （PSII） （O-PSII） and maximum quantum yield of photochemistry in PSII （Fv/Fm）. The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters o-Psii and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation （RSD） of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.
基金supported by the Natural Science Foundation of Anhui Province(No.1708085QD87)the Open Fund of Qingdao National Laboratory for Marine Science and Technology(No.QNLM2016ORP0312)+2 种基金the Natural Science Foundation of Higher Education Institutions of Anhui Province(Nos.KJ2017A530 and KJ2016A594)the National Key Research and Development Plan(No.2016YFC1400602)the Natural Science Foundation of China(No.31400317)
文摘Using a measurement system based on fluorescence induced by variable pulse light, photosynthesis parameters of chlorella pyrenoidosa are obtained, employing single-turnover and multiple-turnover protocols under darkadapted and light-adapted conditions. Under the light-adapted condition,σ’PSII is larger, and F’v/F’m（ST） and F’v/F’m（MT） are smaller than those of the dark-adapted condition, but the corresponding parameters possess good linear correlations.Fm（MT）, F’m（MT）, Fv/Fm（MT） and F’v/F’m（MT） which are measured using the multipleturnover protocol, are larger than those of the single-turnover protocol. The linear correlation coefficient between Fm（ST） and Fm（MT） is 0.984,and Fv/Fm（MT） = 1.18 Fv/Fm（ST） The linear correlation coefficient between F’m（ST） and F’m（MT） is 0.995, and F’v/F’m（MT） = 1.36 F’v/F’m/（ST）.
