Arabidopsis thallana CERK1 is an essential receptor-like kinase in the chitin signal transduction pathway. The juxtamembrane (JM) domain of CERK1 regulates the kinase activity of this receptor. Here we demonstrate tha...Arabidopsis thallana CERK1 is an essential receptor-like kinase in the chitin signal transduction pathway. The juxtamembrane (JM) domain of CERK1 regulates the kinase activity of this receptor. Here we demonstrate that the JM domains of LysM RLKs, CERK1,and OsCERK1 play a functionally conserved role in the activation of chitin signaling in Arabidopsis. The Ctrmini of the JM domains of both CERK1 and OsCERK1 are indispensable for their function. Moreover, after replacing the JM domain of CERK1 with that of the non-homologous RLK, BAK1 (CJBa) or FL52 (CJFI), the chimeric CERK1 receptors maintained their ability to ac.tivate chitin signaling in Arabidopsis. Interestingly, theheterologous expression of CJBa and CJFI did not induce cell death in Nicotiand benthamiand leaves. These results suggest that the JM domains of CERK1, BAK1, and FLS2 play a conserved role in chitin signaling via a mechanism not related to sequence homology.展开更多
Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate pl...Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate plant development and immunity. However, little attention has been paid to their genetic association. This study found the bak1 bik1 double mutant of Arabidopsis displayed a severe dwarfism phenotype due to constitutive immunity and cell death in developing plants. These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death.展开更多
基金supported by the National Natural Science Foundation of China(31571249 and 31425003)。
文摘Arabidopsis thallana CERK1 is an essential receptor-like kinase in the chitin signal transduction pathway. The juxtamembrane (JM) domain of CERK1 regulates the kinase activity of this receptor. Here we demonstrate that the JM domains of LysM RLKs, CERK1,and OsCERK1 play a functionally conserved role in the activation of chitin signaling in Arabidopsis. The Ctrmini of the JM domains of both CERK1 and OsCERK1 are indispensable for their function. Moreover, after replacing the JM domain of CERK1 with that of the non-homologous RLK, BAK1 (CJBa) or FL52 (CJFI), the chimeric CERK1 receptors maintained their ability to ac.tivate chitin signaling in Arabidopsis. Interestingly, theheterologous expression of CJBa and CJFI did not induce cell death in Nicotiand benthamiand leaves. These results suggest that the JM domains of CERK1, BAK1, and FLS2 play a conserved role in chitin signaling via a mechanism not related to sequence homology.
基金supported by the National Natural Science Foundation of China(31571249 and 31425003)
文摘Summary In Arabidopsis, both the membrane-anchored receptor-like kinase （RLK） BAK1 and the receptor-like cytoplasmic kinase （RLCK） BIK1 are important mediators of transmembrane signal transduction that regulate plant development and immunity. However, little attention has been paid to their genetic association. This study found the bak1 bik1 double mutant of Arabidopsis displayed a severe dwarfism phenotype due to constitutive immunity and cell death in developing plants. These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death.
