Biomimetic materials that use natural wisdom to solve practical problems are developing rapidly.The trend for systematic biomimicry is towards in-situ characterization of naturalcreatures with high spatial resolutions...Biomimetic materials that use natural wisdom to solve practical problems are developing rapidly.The trend for systematic biomimicry is towards in-situ characterization of naturalcreatures with high spatial resolutions.Furthermore,rapid reconstruction of digital twin models with the same complex features as the prototype is indispensable.However,it faces bottlenecks and limits in fast characterization and fabrication,precise parameter optimization,geometricdeviations control,and quality prediction.To solve these challenges,here,we demonstrate astate-of-the-art method taking advantage of micro-computed tomography and three-dimensional printing for the fast characterization of the pitcher plant Nepenthes x ventrata and fabrication of its biomimetic model to obtain a superior drainage controller with multiscale structures withprecise surface morphology optimization and geometric deviation control.Thefilm-rupture-based drainage dynamic and mechanisms are characterized by x-ray and high-speed videography,which determines the crucial structures for unique directionaldrainage.Then the optimized artificial pitchers are further developed into sustained drainage devices with novel applications,such as detection,reaction,and smoke control.展开更多
Optical and visual measurement technology is used widely in fields that involve geometric measurements,and among such technology are laser and vision-based displacement measuring modules(LVDMMs).The displacement trans...Optical and visual measurement technology is used widely in fields that involve geometric measurements,and among such technology are laser and vision-based displacement measuring modules(LVDMMs).The displacement transformation coefficient(DTC)of an LVDMM changes with the coordinates in the camera image coordinate system during the displacement measuring process,and these changes affect the displacement measurement accuracy of LVDMMs in the full field of view(FFOV).To give LVDMMs higher accuracy in the FFOV and make them adaptable to widely varying measurement demands,a new calibration method is proposed to improve the displacement measurement accuracy of LVDMMs in the FFOV.First,an image coordinate system,a pixel measurement coordinate system,and a displacement measurement coordinate system are established on the laser receiving screen of the LVDMM.In addition,marker spots in the FFOV are selected,and the DTCs at the marker spots are obtained from calibration experiments.Also,a fitting method based on locally weighted scatterplot smoothing(LOWESS)is selected,and with this fitting method the distribution functions of the DTCs in the FFOV are obtained based on the DTCs at the marker spots.Finally,the calibrated distribution functions of the DTCs are applied to the LVDMM,and experiments conducted to verify the displacement measurement accuracies are reported.The results show that the FFOV measurement accuracies for horizontal and vertical displacements are better than±15μm and±19μm,respectively,and that for oblique displacement is better than±24μm.Compared with the traditional calibration method,the displacement measurement error in the FFOV is now 90%smaller.This research on an improved calibration method has certain significance for improving the measurement accuracy of LVDMMs in the FFOV,and it provides a new method and idea for other vision-based fields in which camera parameters must be calibrated.展开更多
Using hexagonal boron nitride(h-BN)to prepare resistive switching devices is a promising strategy.Various doping methods have aroused great interest in the semiconductor field in recent years,but many researchers have...Using hexagonal boron nitride(h-BN)to prepare resistive switching devices is a promising strategy.Various doping methods have aroused great interest in the semiconductor field in recent years,but many researchers have overlooked the various repetitive anomalies that occur during the testing process.In this study,the basic electrical properties and additive protrusion behavior of Ga-ion-doped h-BN memristors at micro–nanoscale during the voltage scanning process are investigated via atomic force microscopy(AFM)and energy dispersive spectroscopy.The additive protrusion behavior is subjected to exploratory research,and it is concluded that it is caused by anodic oxidation.An approach is proposed that involves filling the AFM chamber with nitrogen gas to improve the stability of memristor testing,and this method provides a solution for enhanced testing stability of memristors.展开更多
Cold atom clocks have made remarkable progresses in the last two decades and played critical roles in precision measurements. Primary Cs fountain frequency standards have achieved a total uncertainty of a few parts in...Cold atom clocks have made remarkable progresses in the last two decades and played critical roles in precision measurements. Primary Cs fountain frequency standards have achieved a total uncertainty of a few parts in 1016, and the best optical clock has reached a type B uncertainty below 10-18. Besides applications in the metrology, navigation, etc.,ultra-stable and ultra-accurate atomic clocks have also become powerful tools in the basic scientific investigations. In this paper, we focus on the recent developments in the high-performance cold atomic clocks which can be used as frequency standards to calibrate atomic time scales. The basic principles, performances, and limitations of fountain clocks and optical clocks based on signal trapped ion or neutral atoms are summarized. Their applications in metrology and other areas are briefly introduced.展开更多
Dynamic tar simulator has been widely used in calibration and validation for star trackers.To achieve both high accuracy and large view field,a dynamic star simulatorbased on optical splicing technology with 2 1920 ...Dynamic tar simulator has been widely used in calibration and validation for star trackers.To achieve both high accuracy and large view field,a dynamic star simulatorbased on optical splicing technology with 2 1920 × 1080- LCDs and a half-transmission and half- reflection prisms was proposed in this paper.The physical principal and error model due to the splicing structure has been discussed and analyzed in detail.Based on the Tsinghua Micro star tracker,the error effect and system accuracy has been carefully tested and calibrated.The result showed that this simulator can get the accuracy of 10.4 "(3sigma) within a field of view of,which can meet the simulating requirement of modern star tracker,whose accuracy can reach 3"(3sigma) 。展开更多
DNA methylation has been extensively investigated in recent years,not least because of its known relationship with various diseases.Progress in analytical methods can greatly increase the relevance of DNA methylation ...DNA methylation has been extensively investigated in recent years,not least because of its known relationship with various diseases.Progress in analytical methods can greatly increase the relevance of DNA methylation studies to both clinical medicine and scientific research.Microflu-idic chips are excellent carriers for molecular analysis,and their use can provide improvements from multiple aspects.On-chip molecular analysis has received extensive attention owing to its advantages of portability,high throughput,low cost,and high efficiency.In recent years,the use of novel microfluidic chips for DNA methylation analysis has been widely reported and has shown obvious superiority to conventional methods.In this review,wefirst focus on DNA methylation and its applications.Then,we discuss advanced microfluidic-based methods for DNA methylation analysis and describe the great progress that has been made in recent years.Finally,we summarize the advantages that microfluidic technology brings to DNA methylation analysis and describe several challenges and perspectives for on-chip DNA methylation analysis.This review should help researchers improve their understanding and make progress in developing microfluidic-based methods for DNA methylation analysis.展开更多
This paper presents the design,fabrication,and characterization of cantilever-type resonators with a novel stacked structure.Aluminum nitride is adopted as the material for both the structural layer and the piezoelect...This paper presents the design,fabrication,and characterization of cantilever-type resonators with a novel stacked structure.Aluminum nitride is adopted as the material for both the structural layer and the piezoelectric layer;this simplifies the fabrication process and improves the quality factor of the resonator.Both in-plane and out-of-planeflexural modes were investigated.The effect of the structural dimensions and electrode patterns on the resonator’s performance were also studied.Finite-element simulations and experiments examining anchor loss and thermoelastic damping,which are the main loss mechanisms affecting the quality factor of these resonators,were carried out.The optimal structural dimensions and electrode patterns of the cantilever-type resonators are presented.A quality factor of 7922 with a motional impedance of 88.52 kΩand a quality factor of 8851 with a motional impedance of 67.03 kΩwere achieved for the in-plane and out-of-planeflexural-mode resonators,respectively.The proposed resonator design will contribute to the development of high-performance devices such as accelerometers,gyroscopes,and pressure sensors.展开更多
Low-fi'equency vertical vibration isolation systems play important roles in precision measurements to reduce seismic and environmental vibration noise. Several types of active vibration isolation systems have been de...Low-fi'equency vertical vibration isolation systems play important roles in precision measurements to reduce seismic and environmental vibration noise. Several types of active vibration isolation systems have been developed. However, few researches focus on how to optimize the test mass install position in order to improve the vibration transmissibility. An active low-frequency vertical vibration isolation system based on an earlier instrument, the Super Spring, is designed and implemented. The system, which is simple and compact, consists of two stages: a parallelogram-shaped linkage to ensure vertical motion, and a simple spring-mass system. The theoretical analysis of the vibration isolation system is presented, including terms erroneously ignored before. By carefully choosing the mechanical parameters according to the above analysis and using feedback control, the resonance frequency of the system is reduced from 2.3 to 0.03 Hz, a reduction by a factor of more than 75. The vibration isolation system is installed as an inertial reference in an absolute gravimeter, where it improved the scatter of the absolute gravity values by a factor of 5. The experimental results verifies the improved performance of the isolation system, making it particularly suitable for precision experiments. The improved vertical vibration isolation system can be used as a prototype for designing high-performance active vertical isolation systems. An improved theoretical model of this active vibration isolation system with beam-pivot configuration is proposed, providing fundamental guidelines for vibration isolator design and assembling.展开更多
The ultraviolet(UV) photoresponses of ZnO nanorods directly grown on and between two micro Au-electrodes by using electric-field-assisted wet chemical method are measured comprehensively under different conditions, ...The ultraviolet(UV) photoresponses of ZnO nanorods directly grown on and between two micro Au-electrodes by using electric-field-assisted wet chemical method are measured comprehensively under different conditions, including ambient environment, applied bias voltage, gate voltage and temperature. Experimental results indicate that the photoresponses of the ZnO nanorods can be modulated by surface oxygen adsorptions, applied voltages, as well as temperatures. A model taking into account both surface adsorbed oxygen and electron-hole activities inside ZnO nanorods is proposed. The enhancement effect of the bias voltage on photoresponse is also analyzed. Experimental results shows that the UV response time(to 63%) of ZnO nanorods in air and at 59°C could be shortened from 34.8 s to 0.24 s with a bias of 4 V applied between anode and cathode.展开更多
The 3D characteristic diagram of acoustically induced surface vibration was employed to study the influence of different buried landmines on the acoustic detection signal. By using the vehicular experimental system fo...The 3D characteristic diagram of acoustically induced surface vibration was employed to study the influence of different buried landmines on the acoustic detection signal. By using the vehicular experimental system for acoustic landmine detection and the method of scanning detection, the 3D characteristic diagrams of surface vibration were measured when different objects were buried underground, including big plastic landmine, small plastic landmine, big metal landmine and bricks. The results show that, under the given conditions, the surface vibration amplitudes of big plastic landmine, big metal landmine, small plastic landmine and bricks decrease in turn. The 3D characteristic diagrams of surface vibration can be used to further identify the locations of buried landmines.展开更多
Fatigue behaviour has important implications for engineering composite structures in sectors ranging from automotive to aerospace. Optical sensing technology displays excellent performance in these fields for monitori...Fatigue behaviour has important implications for engineering composite structures in sectors ranging from automotive to aerospace. Optical sensing technology displays excellent performance in these fields for monitoring. In this paper, temperature and residual strain during fatigue of a carbon fiber reinforced polymer(CFRP) are investigated. Four autoclaved CFRP beam specimens, with fiber Bragg grating(FBG) sensors and thermocouples embedded at selected locations, are subjected to three-point bending cyclic loading on the BOSE testing machine for fatigue testing. Thennocouples are used to measure the temperature while FBGs can sense the temperature and strain as well. Seven tests in total are conducted at different frequencies, and each test lasts for several days. From the experimental results, transient steep peaks of temperature increases (up to 2.3℃) are discovered at the beginning of the load. The following constant temperature increments are around 1.0℃, which is not relevant to frequencies from 0.1 Hz to 20 Hz and suspected due to fatigue. Residual strains of 1×10^-5-2×10^-5 during fatigue, fading away rapidly when unloading, are also reported. Embedded FBGs here are validated to sense temperature and strains in composite structures, which demonstrates promising potentials in structure monitoring fields. CFRP are verified to have an excellent performance during fatigue with low temperature increase and residual strain.展开更多
This paper has developed and characterized a method to produce a velocity-tunable ^87Rb cold atomic source for atomic interferometry application. Using a high speed fluorescence imaging technology, it reports that the...This paper has developed and characterized a method to produce a velocity-tunable ^87Rb cold atomic source for atomic interferometry application. Using a high speed fluorescence imaging technology, it reports that the dynamic process of the atomic source formation is observed and the source performances including the flux and the initial velocity are characterized. A tunable atomic source with the initial velocity of 1.4-2.6 m/s and the atomic source flux of 2× 10^8 - 6 × 10^9 atoms/s has been obtained with the built experimental setup.展开更多
To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the M...To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the MIR range of 3 200-700 cm-1 and NIR range of 9 000-4 000 cm-1.The spectral features in the two regions were analyzed.The MIR spectra of milk were characteristic due to the MIR inherent molecular specificity,whereas the NIR spectra were relatively characterless due to the NIR low selectivity.Partial least squares(PLS)regression models for fat and protein were developed by using both MIR and NIR spectra.MIR data with no pretreatment gave better results than NIR data.The square correlation coefficient(R2)and the root mean square error of prediction(RMSEP)were 0.98 and 0.10 g/dL for fat and 0.97 and 0.11 g/dL for protein.With NIR techniques,satisfactory results were not obtained with raw data.However,NIR data after pretreatment gave similarly good results to the ones using MIR method.This paper indicates that either of the MIR and NIR spectral methods is reliable for the determination of the fat and protein contents.展开更多
As actuator of the force-rebalanced servo loop, the electrostatic force generator of the micro-accelerometer shows high nonlinearity while the interpole of the micro-electro-mechanical system(MEMS) sensor is far awa...As actuator of the force-rebalanced servo loop, the electrostatic force generator of the micro-accelerometer shows high nonlinearity while the interpole of the micro-electro-mechanical system(MEMS) sensor is far away from its balance position. The control system cannot rebalance itself with the limited bandwidth after an external long overload, because the characteristics of the force generator differ from normal case. Although for similar problems, solutions with cascading lead-lag blocks, with the anti-windup(AW) technology, or with the sliding-mode control, are widely reported, the problems such as performance loss or difficulty to synthesize a digital controller still remain. Based on existing researches, remedies are developed by analyzing the characteristic of the system not only near the balance position, but also corresponding to the whole moveable range of the interpole, and a new controller is proposed. The solution is compared with the common solutions of cascading lead-lag blocks method, AW methods, and sliding mode methods. Comparison results show that the proposed solution avoid performance loss, compared to cascading lead-lag blocks solution; the proposed solution is easily synthesized and implemented in the analog servo loop of the micro-accelerometer, compared to digital AW methods; at the same time, the proposed solution avoids suffering the chattering effect problem but just utilize it, compared to the sliding-mode control solution. Nevertheless, comparison results show the solution is lack of commonality, since the solution is only more suitable to micro electrostatic force-rebalance system. The SIMULINK models with and without the proposed solution, taking typical micro-accelerometer parameters, have been set up for simulation; corresponding experiments utilizing electrometric method are also conducted after the successful simulations. Simulation and experiment results verify that the micro-accelerometer will reliably return to normal operation after external long overload with the proposed solution. Therefore, it is expected to design the analog servo loop of high performance micro electrostatic force-rebalance system so as to ensure the rebalance after long overload without performance loss.展开更多
External-cavity birefringence feedback effects of the microchip Nd:YAG laser are presented. When a birefringence element is placed in the external feedback cavity of the laser, two orthogonally polarized laser beams ...External-cavity birefringence feedback effects of the microchip Nd:YAG laser are presented. When a birefringence element is placed in the external feedback cavity of the laser, two orthogonally polarized laser beams with a phase difference are output. The phase difference is twice as large as the phase retardation in the external cavity along the two orthogonal directions. The variable extra-cavity birefringence, caused by rotation of the external-cavity birefringenee element, results in tunable phase difference between the two orthogonally polarized beams. This means that the roll angle information has been translated to phase difference of two output laser beams. A theoretical analysis based on the Fabry-Perot cavity equivalent model and refractive index ellipsoid is presented, which is in good agreement with the experimental results. This phenomenon has potential applications for roll angle measurement.展开更多
The surface acoustic wave (SAW) propagating in a sample of steel is simulated by using finite element method (FEM). The waves are excited by a load function with propagation properties such as phase velocity dispersio...The surface acoustic wave (SAW) propagating in a sample of steel is simulated by using finite element method (FEM). The waves are excited by a load function with propagation properties such as phase velocity dispersion and wide bandwidth. A two-dimensional model consisting of surface defects loaded with a wideband 50-200 MHz and short time 0.1 μs displacement function is investigated in the time and frequency domains. By transient dynamic analysis, Fourier transform and dispersion calculation, snapshots of propagating wave and responses from sensing points are presented. It is indicated that this supervision approach is sensitive to the surface cracks and reflections.展开更多
This paper reports on a method of assembling semiconducting ZnO nanowires onto a pair of Au electrodes to construct a metal--semiconductor metal (MSM) structure by dieleetrophoresis and studying on its electrical ch...This paper reports on a method of assembling semiconducting ZnO nanowires onto a pair of Au electrodes to construct a metal--semiconductor metal (MSM) structure by dieleetrophoresis and studying on its electrical characteristics by using current-voltage (Ⅰ - Ⅴ) measurements. An electronic model with two back to back Sehottky diodes in series with a semiconductor of nanowires was established to study the electrical transport of the MSM structures. By fitting the measured Ⅰ - Ⅴ characteristics using the proposed model, the parameters of the Schottky contacts and the resistance of nanowires could be acquired. The photoelectric properties of the MSM structures were also investigated by analysing the measurements of the electrical transports under various light intensities. The deduced results demonstrate that ZnO nanowires and their Schottky contacts with Au electrodes both contribute to photosensitivity and the MSM structures with ZnO nanowires are potentially applicable for photonic devices.展开更多
Alkaline phosphatase(ALP) plays an integral role in the metabolism of liver and development of the skeleton in humans. To date, the interactions between different-duration terahertz(THz) radiation and ALP activities, ...Alkaline phosphatase(ALP) plays an integral role in the metabolism of liver and development of the skeleton in humans. To date, the interactions between different-duration terahertz(THz) radiation and ALP activities, as well as the influence mechanism are still unclear. In this study, using the para-nitro-phenyl-phosphate(p NPP) method, we detect changes in ALP activities during 40-minute THz radiation(0.1 THz, 13 m W/cm^2). It is found that the activity of ALP decreases in the first 25 min, and subsequently increases in the later 15 min. Compared with the activity of ALP being heated, the results suggest that short-term terahertz radiation induces a decrease in enzyme activity through the non-thermal mechanism. In order to explore the non-thermal effects of THz radiation on ALP, we focus on the impacts of 0.1 THz radiation for 20 min on the activity of ALP in different concentrations. The results reveal that the activity of ALP decreases significantly after exposure to THz radiation. In addition, it could be deduced from fluorescence, ultraviolet-visible(UV-vis), and THz spectra results that THz radiation has induced changes in ALP structures. Our study unlocks non-thermal interactions between THz radiation and ALP, as well as suggests that THz spectroscopy is a promising technique to distinguish ALP structures.展开更多
基金provided by the National sKey R&D Program of China(2021YFA0716701)the National Natural Science Foundation of China(22005014,.22275007,22102204)+1 种基金Beihang University’s Young Talents(No.KG16164901)Open Foundation of the State Key Laboratory of Precision Measuring Technology and Instruments(No.pilab2106)。
文摘Biomimetic materials that use natural wisdom to solve practical problems are developing rapidly.The trend for systematic biomimicry is towards in-situ characterization of naturalcreatures with high spatial resolutions.Furthermore,rapid reconstruction of digital twin models with the same complex features as the prototype is indispensable.However,it faces bottlenecks and limits in fast characterization and fabrication,precise parameter optimization,geometricdeviations control,and quality prediction.To solve these challenges,here,we demonstrate astate-of-the-art method taking advantage of micro-computed tomography and three-dimensional printing for the fast characterization of the pitcher plant Nepenthes x ventrata and fabrication of its biomimetic model to obtain a superior drainage controller with multiscale structures withprecise surface morphology optimization and geometric deviation control.Thefilm-rupture-based drainage dynamic and mechanisms are characterized by x-ray and high-speed videography,which determines the crucial structures for unique directionaldrainage.Then the optimized artificial pitchers are further developed into sustained drainage devices with novel applications,such as detection,reaction,and smoke control.
基金supported financially by the National Natural Science Foundation of China (NSFC) (Grant No.51775378)the Key Projects in Tianjin Science&Technology Support Program (Grant No.19YFZC GX00890).
文摘Optical and visual measurement technology is used widely in fields that involve geometric measurements,and among such technology are laser and vision-based displacement measuring modules(LVDMMs).The displacement transformation coefficient(DTC)of an LVDMM changes with the coordinates in the camera image coordinate system during the displacement measuring process,and these changes affect the displacement measurement accuracy of LVDMMs in the full field of view(FFOV).To give LVDMMs higher accuracy in the FFOV and make them adaptable to widely varying measurement demands,a new calibration method is proposed to improve the displacement measurement accuracy of LVDMMs in the FFOV.First,an image coordinate system,a pixel measurement coordinate system,and a displacement measurement coordinate system are established on the laser receiving screen of the LVDMM.In addition,marker spots in the FFOV are selected,and the DTCs at the marker spots are obtained from calibration experiments.Also,a fitting method based on locally weighted scatterplot smoothing(LOWESS)is selected,and with this fitting method the distribution functions of the DTCs in the FFOV are obtained based on the DTCs at the marker spots.Finally,the calibrated distribution functions of the DTCs are applied to the LVDMM,and experiments conducted to verify the displacement measurement accuracies are reported.The results show that the FFOV measurement accuracies for horizontal and vertical displacements are better than±15μm and±19μm,respectively,and that for oblique displacement is better than±24μm.Compared with the traditional calibration method,the displacement measurement error in the FFOV is now 90%smaller.This research on an improved calibration method has certain significance for improving the measurement accuracy of LVDMMs in the FFOV,and it provides a new method and idea for other vision-based fields in which camera parameters must be calibrated.
基金supported by the Youth Fund of the National Natural Science Foundation of China(Grant No.622041701004267).
文摘Using hexagonal boron nitride(h-BN)to prepare resistive switching devices is a promising strategy.Various doping methods have aroused great interest in the semiconductor field in recent years,but many researchers have overlooked the various repetitive anomalies that occur during the testing process.In this study,the basic electrical properties and additive protrusion behavior of Ga-ion-doped h-BN memristors at micro–nanoscale during the voltage scanning process are investigated via atomic force microscopy(AFM)and energy dispersive spectroscopy.The additive protrusion behavior is subjected to exploratory research,and it is concluded that it is caused by anodic oxidation.An approach is proposed that involves filling the AFM chamber with nitrogen gas to improve the stability of memristor testing,and this method provides a solution for enhanced testing stability of memristors.
基金Project supported by the National Natural Science Foundation of China(Grant No.11873044)the National Key Research and Development Project of China(Grant No.2016YFF0200202)Consulting Research Project of Chinese Academy of Engineering(Grant No.2018-ZCQ-03)。
文摘Cold atom clocks have made remarkable progresses in the last two decades and played critical roles in precision measurements. Primary Cs fountain frequency standards have achieved a total uncertainty of a few parts in 1016, and the best optical clock has reached a type B uncertainty below 10-18. Besides applications in the metrology, navigation, etc.,ultra-stable and ultra-accurate atomic clocks have also become powerful tools in the basic scientific investigations. In this paper, we focus on the recent developments in the high-performance cold atomic clocks which can be used as frequency standards to calibrate atomic time scales. The basic principles, performances, and limitations of fountain clocks and optical clocks based on signal trapped ion or neutral atoms are summarized. Their applications in metrology and other areas are briefly introduced.
文摘Dynamic tar simulator has been widely used in calibration and validation for star trackers.To achieve both high accuracy and large view field,a dynamic star simulatorbased on optical splicing technology with 2 1920 × 1080- LCDs and a half-transmission and half- reflection prisms was proposed in this paper.The physical principal and error model due to the splicing structure has been discussed and analyzed in detail.Based on the Tsinghua Micro star tracker,the error effect and system accuracy has been carefully tested and calibrated.The result showed that this simulator can get the accuracy of 10.4 "(3sigma) within a field of view of,which can meet the simulating requirement of modern star tracker,whose accuracy can reach 3"(3sigma) 。
基金support from the National Key R&D Program of China(Grant No.2018YFE0118700)the National Natural Science Foundation of China(NSFC Grant No.62174119)+1 种基金the 111 Project(Grant No.B07014)the Foundation for Talent Scientists of Nanchang Institute for Microtechnology of Tianjin University.
文摘DNA methylation has been extensively investigated in recent years,not least because of its known relationship with various diseases.Progress in analytical methods can greatly increase the relevance of DNA methylation studies to both clinical medicine and scientific research.Microflu-idic chips are excellent carriers for molecular analysis,and their use can provide improvements from multiple aspects.On-chip molecular analysis has received extensive attention owing to its advantages of portability,high throughput,low cost,and high efficiency.In recent years,the use of novel microfluidic chips for DNA methylation analysis has been widely reported and has shown obvious superiority to conventional methods.In this review,wefirst focus on DNA methylation and its applications.Then,we discuss advanced microfluidic-based methods for DNA methylation analysis and describe the great progress that has been made in recent years.Finally,we summarize the advantages that microfluidic technology brings to DNA methylation analysis and describe several challenges and perspectives for on-chip DNA methylation analysis.This review should help researchers improve their understanding and make progress in developing microfluidic-based methods for DNA methylation analysis.
基金supported in part by the National Key Research and Development Program of China(Grant No.2020YFB2008800)in part by the Nanchang Institute for Microtechnology of Tianjin University.
文摘This paper presents the design,fabrication,and characterization of cantilever-type resonators with a novel stacked structure.Aluminum nitride is adopted as the material for both the structural layer and the piezoelectric layer;this simplifies the fabrication process and improves the quality factor of the resonator.Both in-plane and out-of-planeflexural modes were investigated.The effect of the structural dimensions and electrode patterns on the resonator’s performance were also studied.Finite-element simulations and experiments examining anchor loss and thermoelastic damping,which are the main loss mechanisms affecting the quality factor of these resonators,were carried out.The optimal structural dimensions and electrode patterns of the cantilever-type resonators are presented.A quality factor of 7922 with a motional impedance of 88.52 kΩand a quality factor of 8851 with a motional impedance of 67.03 kΩwere achieved for the in-plane and out-of-planeflexural-mode resonators,respectively.The proposed resonator design will contribute to the development of high-performance devices such as accelerometers,gyroscopes,and pressure sensors.
基金Supported by Tsinghua University’s Scientific Research Initiative Program,China(Grant No.2010THZ05)
文摘Low-fi'equency vertical vibration isolation systems play important roles in precision measurements to reduce seismic and environmental vibration noise. Several types of active vibration isolation systems have been developed. However, few researches focus on how to optimize the test mass install position in order to improve the vibration transmissibility. An active low-frequency vertical vibration isolation system based on an earlier instrument, the Super Spring, is designed and implemented. The system, which is simple and compact, consists of two stages: a parallelogram-shaped linkage to ensure vertical motion, and a simple spring-mass system. The theoretical analysis of the vibration isolation system is presented, including terms erroneously ignored before. By carefully choosing the mechanical parameters according to the above analysis and using feedback control, the resonance frequency of the system is reduced from 2.3 to 0.03 Hz, a reduction by a factor of more than 75. The vibration isolation system is installed as an inertial reference in an absolute gravimeter, where it improved the scatter of the absolute gravity values by a factor of 5. The experimental results verifies the improved performance of the isolation system, making it particularly suitable for precision experiments. The improved vertical vibration isolation system can be used as a prototype for designing high-performance active vertical isolation systems. An improved theoretical model of this active vibration isolation system with beam-pivot configuration is proposed, providing fundamental guidelines for vibration isolator design and assembling.
基金Project supported by the National Natural Science Foundation of China(Grant No.91123017)
文摘The ultraviolet(UV) photoresponses of ZnO nanorods directly grown on and between two micro Au-electrodes by using electric-field-assisted wet chemical method are measured comprehensively under different conditions, including ambient environment, applied bias voltage, gate voltage and temperature. Experimental results indicate that the photoresponses of the ZnO nanorods can be modulated by surface oxygen adsorptions, applied voltages, as well as temperatures. A model taking into account both surface adsorbed oxygen and electron-hole activities inside ZnO nanorods is proposed. The enhancement effect of the bias voltage on photoresponse is also analyzed. Experimental results shows that the UV response time(to 63%) of ZnO nanorods in air and at 59°C could be shortened from 34.8 s to 0.24 s with a bias of 4 V applied between anode and cathode.
基金Supported by the National Natural Science Foundation of China(No.61575119)Science and Technology on Near-Surface Detection Laboratory(No.TCGZ2015A005)State Key Laboratory of Precision Measuring Technology and Instruments(PIL1402)
文摘The 3D characteristic diagram of acoustically induced surface vibration was employed to study the influence of different buried landmines on the acoustic detection signal. By using the vehicular experimental system for acoustic landmine detection and the method of scanning detection, the 3D characteristic diagrams of surface vibration were measured when different objects were buried underground, including big plastic landmine, small plastic landmine, big metal landmine and bricks. The results show that, under the given conditions, the surface vibration amplitudes of big plastic landmine, big metal landmine, small plastic landmine and bricks decrease in turn. The 3D characteristic diagrams of surface vibration can be used to further identify the locations of buried landmines.
文摘Fatigue behaviour has important implications for engineering composite structures in sectors ranging from automotive to aerospace. Optical sensing technology displays excellent performance in these fields for monitoring. In this paper, temperature and residual strain during fatigue of a carbon fiber reinforced polymer(CFRP) are investigated. Four autoclaved CFRP beam specimens, with fiber Bragg grating(FBG) sensors and thermocouples embedded at selected locations, are subjected to three-point bending cyclic loading on the BOSE testing machine for fatigue testing. Thennocouples are used to measure the temperature while FBGs can sense the temperature and strain as well. Seven tests in total are conducted at different frequencies, and each test lasts for several days. From the experimental results, transient steep peaks of temperature increases (up to 2.3℃) are discovered at the beginning of the load. The following constant temperature increments are around 1.0℃, which is not relevant to frequencies from 0.1 Hz to 20 Hz and suspected due to fatigue. Residual strains of 1×10^-5-2×10^-5 during fatigue, fading away rapidly when unloading, are also reported. Embedded FBGs here are validated to sense temperature and strains in composite structures, which demonstrates promising potentials in structure monitoring fields. CFRP are verified to have an excellent performance during fatigue with low temperature increase and residual strain.
基金supported in part by National Natural Science Foundation of China (Grant No 50775127/E0525)National Basic Research Specialized Program of China (Grant No 2007CB306504)
文摘This paper has developed and characterized a method to produce a velocity-tunable ^87Rb cold atomic source for atomic interferometry application. Using a high speed fluorescence imaging technology, it reports that the dynamic process of the atomic source formation is observed and the source performances including the flux and the initial velocity are characterized. A tunable atomic source with the initial velocity of 1.4-2.6 m/s and the atomic source flux of 2× 10^8 - 6 × 10^9 atoms/s has been obtained with the built experimental setup.
基金Supported by National Natural Science Foundation of China(No.30170261)the 10th Five-Year Plan of China(No.2004BA706B12).
文摘To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the MIR range of 3 200-700 cm-1 and NIR range of 9 000-4 000 cm-1.The spectral features in the two regions were analyzed.The MIR spectra of milk were characteristic due to the MIR inherent molecular specificity,whereas the NIR spectra were relatively characterless due to the NIR low selectivity.Partial least squares(PLS)regression models for fat and protein were developed by using both MIR and NIR spectra.MIR data with no pretreatment gave better results than NIR data.The square correlation coefficient(R2)and the root mean square error of prediction(RMSEP)were 0.98 and 0.10 g/dL for fat and 0.97 and 0.11 g/dL for protein.With NIR techniques,satisfactory results were not obtained with raw data.However,NIR data after pretreatment gave similarly good results to the ones using MIR method.This paper indicates that either of the MIR and NIR spectral methods is reliable for the determination of the fat and protein contents.
基金supported by National Key Technologies R&D Program of China during the 11th Five-Year Plan (Grant No. 51309050208)
文摘As actuator of the force-rebalanced servo loop, the electrostatic force generator of the micro-accelerometer shows high nonlinearity while the interpole of the micro-electro-mechanical system(MEMS) sensor is far away from its balance position. The control system cannot rebalance itself with the limited bandwidth after an external long overload, because the characteristics of the force generator differ from normal case. Although for similar problems, solutions with cascading lead-lag blocks, with the anti-windup(AW) technology, or with the sliding-mode control, are widely reported, the problems such as performance loss or difficulty to synthesize a digital controller still remain. Based on existing researches, remedies are developed by analyzing the characteristic of the system not only near the balance position, but also corresponding to the whole moveable range of the interpole, and a new controller is proposed. The solution is compared with the common solutions of cascading lead-lag blocks method, AW methods, and sliding mode methods. Comparison results show that the proposed solution avoid performance loss, compared to cascading lead-lag blocks solution; the proposed solution is easily synthesized and implemented in the analog servo loop of the micro-accelerometer, compared to digital AW methods; at the same time, the proposed solution avoids suffering the chattering effect problem but just utilize it, compared to the sliding-mode control solution. Nevertheless, comparison results show the solution is lack of commonality, since the solution is only more suitable to micro electrostatic force-rebalance system. The SIMULINK models with and without the proposed solution, taking typical micro-accelerometer parameters, have been set up for simulation; corresponding experiments utilizing electrometric method are also conducted after the successful simulations. Simulation and experiment results verify that the micro-accelerometer will reliably return to normal operation after external long overload with the proposed solution. Therefore, it is expected to design the analog servo loop of high performance micro electrostatic force-rebalance system so as to ensure the rebalance after long overload without performance loss.
基金supported by the National Natural Science Foundation of China (Grant No 50575110)
文摘External-cavity birefringence feedback effects of the microchip Nd:YAG laser are presented. When a birefringence element is placed in the external feedback cavity of the laser, two orthogonally polarized laser beams with a phase difference are output. The phase difference is twice as large as the phase retardation in the external cavity along the two orthogonal directions. The variable extra-cavity birefringence, caused by rotation of the external-cavity birefringenee element, results in tunable phase difference between the two orthogonally polarized beams. This means that the roll angle information has been translated to phase difference of two output laser beams. A theoretical analysis based on the Fabry-Perot cavity equivalent model and refractive index ellipsoid is presented, which is in good agreement with the experimental results. This phenomenon has potential applications for roll angle measurement.
基金Supported by National Natural Science Foundation of China (No. 51075296)Sate Key Laboratory of Precision Measuring Technology and Instruments Project (No. PILT1106)
文摘The surface acoustic wave (SAW) propagating in a sample of steel is simulated by using finite element method (FEM). The waves are excited by a load function with propagation properties such as phase velocity dispersion and wide bandwidth. A two-dimensional model consisting of surface defects loaded with a wideband 50-200 MHz and short time 0.1 μs displacement function is investigated in the time and frequency domains. By transient dynamic analysis, Fourier transform and dispersion calculation, snapshots of propagating wave and responses from sensing points are presented. It is indicated that this supervision approach is sensitive to the surface cracks and reflections.
基金Project supported by the National Natural Science Foundation of China (Grant No 50575113)Program for NCET
文摘This paper reports on a method of assembling semiconducting ZnO nanowires onto a pair of Au electrodes to construct a metal--semiconductor metal (MSM) structure by dieleetrophoresis and studying on its electrical characteristics by using current-voltage (Ⅰ - Ⅴ) measurements. An electronic model with two back to back Sehottky diodes in series with a semiconductor of nanowires was established to study the electrical transport of the MSM structures. By fitting the measured Ⅰ - Ⅴ characteristics using the proposed model, the parameters of the Schottky contacts and the resistance of nanowires could be acquired. The photoelectric properties of the MSM structures were also investigated by analysing the measurements of the electrical transports under various light intensities. The deduced results demonstrate that ZnO nanowires and their Schottky contacts with Au electrodes both contribute to photosensitivity and the MSM structures with ZnO nanowires are potentially applicable for photonic devices.
基金Project supported by the National Natural Science Foundation of China(Grant No.61675151)
文摘Alkaline phosphatase(ALP) plays an integral role in the metabolism of liver and development of the skeleton in humans. To date, the interactions between different-duration terahertz(THz) radiation and ALP activities, as well as the influence mechanism are still unclear. In this study, using the para-nitro-phenyl-phosphate(p NPP) method, we detect changes in ALP activities during 40-minute THz radiation(0.1 THz, 13 m W/cm^2). It is found that the activity of ALP decreases in the first 25 min, and subsequently increases in the later 15 min. Compared with the activity of ALP being heated, the results suggest that short-term terahertz radiation induces a decrease in enzyme activity through the non-thermal mechanism. In order to explore the non-thermal effects of THz radiation on ALP, we focus on the impacts of 0.1 THz radiation for 20 min on the activity of ALP in different concentrations. The results reveal that the activity of ALP decreases significantly after exposure to THz radiation. In addition, it could be deduced from fluorescence, ultraviolet-visible(UV-vis), and THz spectra results that THz radiation has induced changes in ALP structures. Our study unlocks non-thermal interactions between THz radiation and ALP, as well as suggests that THz spectroscopy is a promising technique to distinguish ALP structures.