Calibration of line structured light vision system based on camera’s projective center

Based on the characteristics of line structured light sensor, a speedy method for the calibration was established. With the coplanar reference target, the spacial pose between camera and optical plane can be calibrated by using of the camera’s projective center and the light’s information in the camera’s image surface. Without striction to the movement of the coplanar reference target and assistant adjustment equipment, this calibration method can be implemented. This method has been used and decreased the cost of calibration equipment, simplified the calibration procedure, improved calibration efficiency. Using experiment, the sensor can attain relative accuracy about 0.5%, which indicates the rationality and effectivity of this method.

Applying the reference-wavelength method to improve the precision of glucose measurement by near infrared spectroscopy

The reference-wavelength method is proposed to diminish the influence of noises on glucose measurement by differentially processing two signals at the reference and measuring wavelengths. At the reference wavelength, the radiation intensity is insensitive to the changes of glucose concentration. Therefore, it can be used as the internal reference to estimate the noise and then to extract the effective glucose signal at the other wavelengths. The validation experiments are constructed in the non-scattering samples with the reference wavelength of glucose at 1525 nm. The results show that the reference-wavelength-based glucose-specific signal extracting method can largely improve the glucose prediction precision from 17.56 to 8.87 mg/dL in the two-component experiment aad from 26.82 to 9.94 mg/dL in the three-component experiment.

Reference point of floating-reference method for blood glucose sensing

A problem in terms of the accuracy of noninvasive measurement of blood glucose with near-infrared（NIR） spectroscopy is mainly caused by the weak glucose signal and strong background variations.We report the existence of the radial reference point in a floating-reference method,which is supposed to solve this problem.Based on the analysis of the infinite diffusion theory,the local condition of the reference point is deduced theoretically.Then the experiments using the intralipid solutions are constructed to testify the existence of the reference point.In order to further validate our results,Monte Carlo simulations are performed to calculate the diffused light distribution according to the variation of the glucose concentration in the intralipid solutions.All the reference points existing in three-layer skin model are also listed at the wavelength of 1200-1700 nm.

Propulsion Principles of Water Striders in Sculling Forward through Shadow Method

Semi-aquatic arthropods skate on water surfaces with synergetic actions of their legs. The sculling forward locomotion of water striders was observed and analyzed in situ to understand and reproduce the abovementioned feature. The bright-edged elliptical shadows of the six legs of a water strider were recorded to derive the supporting force distributions on legs. The propulsion principles of water striders were quantitatively disclosed. A typical sculling forward process was accomplished within approximately 0.15 s. Water striders lifted their heads slightly and supported their weight mainly by the two driving legs to increase the propulsion force and reduce the water resistance during the process. The normalized thrust-area ratio （defined as the ratio of the propulsion force to the projected area） was usually lower than 0.4 after sculling for approximately 0.08 s. The entire normal supporting force remained nearly constant during a stroke to reduce the mass center fluctuation in the normal direction. In addition, water striders could easily control the locomotion direction and speed through the light swinging of the two hind legs as rudders. These sculling principles might inspire sophisticated biomimetic wa- ter-walking robots with high propulsion efficiency in the future.

Calibration error analysis of electro-optical detection systems

We present detailed analysis of calibration process error for electro-optical detection systems, which can be simplified as the plane rotation around a non-orthogonal axis. By means of octonions it firstly proves that the plane rotation around a non-orthogonal axis can be decomposed into rotations around two perpendicular axes. The rotation is further divided into three steps, and the calibration error is hence discussed and obtained. The simulation and test results indicate that there are large calibration errors in calibration process. The pointing error can be effectively improved after separating error components, which provides a more accurate set data for further comDensation.

A New Information Method to Determinate Corrosion Types from Electrochemical Noise

Electrochemical noise data in the presence of different corrosion types were analyzed. A correlation between the corrosion type of the observed noise and the entropy rate was recognized. A sequence was transformed from each set of original electrochemical noise signal, then the Markov process M and stationary distribution μ were calculated. Finally, entropy rate of each sequence was obtained by the definition. From the entropy rate, it has been observed that local corrosion showed a lower entropy rate, while uniform corrosion showed a higher entropy rate.

Net analyte signal with floating reference theory in non-invasive blood glucose sensing by near-infrared spectroscopy

Based on the floating reference theory, a new method for extracting the net analyte signal （NAS） is proposed. The noise background subspace is spanned by spectra at the floating radial reference point, and then, the spectra at the measurement point are projected on the subspace. Thereafter, the glucose concentrations in intralipid solutions are investigated through Monte Carlo simulation and experiments, and the partial least squares （PLS） models with and without NAS analysis are built. The root mean square errors of calibration and prediction reach to 28.87% and 27.33%, respectively. The results confirm the existence of information induced by glucose concentration variations as well as the validity of the floating reference theory.

Single-cell Analysis Technologies for Immuno-oncology Research:from Mechanistic Delineation to Biomarker Discovery

The successes with immune checkpoint blockade(ICB)and chimeric antigen receptor(CAR)-T-cell therapy in treating multiple cancer types have established immunotherapy as a powerful curative option for patients with advanced cancers.Unfortunately,many patients do not derive benefit or long-term responses,highlighting a pressing need to perform complete investigation of the underlying mechanisms and the immunotherapy-induced tumor regression or rejection.In recent years,a large number of single-cell technologies have leveraged advances in characterizing immune system,profiling tumor microenvironment,and identifying cellular heterogeneity,which establish the foundations for lifting the veil on the comprehensive crosstalk between cancer and immune system during immunotherapies.In this review,we introduce the applications of the most widely used single-cell technologies in furthering our understanding of immunotherapies in terms of underlying mechanisms and their association with therapeutic outcomes.We also discuss how single-cell analyses help to deliver new insights into biomarker discovery to predict patient response rate,monitor acquired resistance,and support prophylactic strategy development for toxicity management.Finally,we provide an overview of applying cutting-edge single-cell spatial-omics to point out the heterogeneity of tumor–immune interactions at higher level that can ultimately guide to the rational design of next-generation immunotherapies.