AIM: To find out key genes responsible for hepatocarc inogenesis and to further understand the underlying molecular mechanism through investigating the differential gene expression between human normal liver tissue an...AIM: To find out key genes responsible for hepatocarc inogenesis and to further understand the underlying molecular mechanism through investigating the differential gene expression between human normal liver tissue and hepatocellular carcinoma (HCC).METHODS: DNA microarray was prepared by spotting PCR products of 1 000 human genes including 445 novel genes, 540 known genes as well as 12 positive (housekeeping) and 3 negative controls (plant gene) onto treated glass slides. cDNA probes were prepared by labeling normal liver tissue mRNA and cancer liver tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The arrays were hybridized against the cDNA probe and the fluorescent signals were scanned. The dataobtained from repeated experiments were analyzed. RESULTS: Among the 20 couple samples investigated (from cancerous liver tissue and normal liver tissue), 38 genes including 21 novel genes and 17 known genes exhibited different expressions. CONCLUSION: cDNA microarray technique is powerful to identify candidate target genes that may play important roles in human carcinogenesis. Further analysis of the obtained genes is helpful to understand the molecular changes in HCC progression and ultimately may lead to the identification of new targets for HCC diagnosis and intervention.展开更多
Multimode interference (MMI) devices are widely used in planar lightwave circuit (PLC). The device length can be well reduced with tapered multimode region. Traditional design formula shows large error for MMI devices...Multimode interference (MMI) devices are widely used in planar lightwave circuit (PLC). The device length can be well reduced with tapered multimode region. Traditional design formula shows large error for MMI devices based on weakly- restricted waveguide. Based on the analysis with mode width as a substitution of waveguide width, a design formula with better precision was presented. Comparison with software simulation verified its exactness.展开更多
Aiming at boosting the low ultraviolet (UV)re-sponsivity induced by thenegative impact of the surface 'dead layer' in silicon-based conventional photodiode (CPD), Siphotodiodes with five different structures, ...Aiming at boosting the low ultraviolet (UV)re-sponsivity induced by thenegative impact of the surface 'dead layer' in silicon-based conventional photodiode (CPD), Siphotodiodes with five different structures, including both the novel grid structurephotodiode(GSPD)and CPD, have been manufactured using thermal diffusion process and tested. The results show thatthe UV responsivity around 365 nm of GSPD could be as high as 6 times that of CPD, while the highvisible (VIS) responsivity is sharply suppressed by the employment of grid shaped junction (GSJ) inthe GSPD, which has realized the expectation of selective UV enhancement with prospect forapplication.展开更多
基金Supported by the Special Fund of Chinese Academy of Sciences, No. KSCX1-06
文摘AIM: To find out key genes responsible for hepatocarc inogenesis and to further understand the underlying molecular mechanism through investigating the differential gene expression between human normal liver tissue and hepatocellular carcinoma (HCC).METHODS: DNA microarray was prepared by spotting PCR products of 1 000 human genes including 445 novel genes, 540 known genes as well as 12 positive (housekeeping) and 3 negative controls (plant gene) onto treated glass slides. cDNA probes were prepared by labeling normal liver tissue mRNA and cancer liver tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The arrays were hybridized against the cDNA probe and the fluorescent signals were scanned. The dataobtained from repeated experiments were analyzed. RESULTS: Among the 20 couple samples investigated (from cancerous liver tissue and normal liver tissue), 38 genes including 21 novel genes and 17 known genes exhibited different expressions. CONCLUSION: cDNA microarray technique is powerful to identify candidate target genes that may play important roles in human carcinogenesis. Further analysis of the obtained genes is helpful to understand the molecular changes in HCC progression and ultimately may lead to the identification of new targets for HCC diagnosis and intervention.
文摘Multimode interference (MMI) devices are widely used in planar lightwave circuit (PLC). The device length can be well reduced with tapered multimode region. Traditional design formula shows large error for MMI devices based on weakly- restricted waveguide. Based on the analysis with mode width as a substitution of waveguide width, a design formula with better precision was presented. Comparison with software simulation verified its exactness.
文摘Aiming at boosting the low ultraviolet (UV)re-sponsivity induced by thenegative impact of the surface 'dead layer' in silicon-based conventional photodiode (CPD), Siphotodiodes with five different structures, including both the novel grid structurephotodiode(GSPD)and CPD, have been manufactured using thermal diffusion process and tested. The results show thatthe UV responsivity around 365 nm of GSPD could be as high as 6 times that of CPD, while the highvisible (VIS) responsivity is sharply suppressed by the employment of grid shaped junction (GSJ) inthe GSPD, which has realized the expectation of selective UV enhancement with prospect forapplication.
