This study was performed in order to determine visitation at remote areas that would be unaffordable due to logistic reasons. Four TrafxTM vehicle counters, each programmed with different settings, were placed along t...This study was performed in order to determine visitation at remote areas that would be unaffordable due to logistic reasons. Four TrafxTM vehicle counters, each programmed with different settings, were placed along the lone access road to remotely sense the daily use activities and count accuracy at the New Underwood Lake Public Water Access Area. Use was corroborated during daylight hours with game cameras. Data was stratified between weekdays/weekends due to differences between the two periods. Two counter settings, threshold and delay, were best when set at a value of 8, but a value of 16 for delay provided almost equal results. Overall, there were 38 counts of use per day for a total of 2318 over the 61 day period. This study demonstrated how vehicle counters, in combination with game cameras for verification, can aid managers for determining use in remote access areas. Future work may lead to identifying details for producing a surrogate to traditional angler use surveys.展开更多
Objective To discuss the problems existing in the compensation of Chinese clinical trial participants and propose some suggestions for improving their rights.Methods The literature related to the participants’right t...Objective To discuss the problems existing in the compensation of Chinese clinical trial participants and propose some suggestions for improving their rights.Methods The literature related to the participants’right to compensation at home and abroad was searched to study the inadequacy of the compensation right for clinical trial participants in China from four aspects:insurance system,principle of attribution,legal relationship and compensation regulations.Then,some suggestions to improve the participants’right to compensation were proposed.Results and Conclusion China lacks clear legal norms for participants’right to compensation.There are problems such as unclear insurance rules and compensation rules,unclear contractual relationships between parties to clinical trials,and no laws and regulations to rely on for attribution and compensation.China should issue regulatory guidelines related to the right to compensation of participants in clinical trials,so that all parties in clinical trials can have rules to follow if there is the occurrence of injury,which can better protect the rights and interests of the participants.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharid...BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharides.MJF has been used in the clinical treatment of hepatitis,cirrhosis and HCC for more than 30 years.Few previous studies have focused on the mechanism of MJF on tumor immunology in the treatment of HCC.AIM To explore the mechanism of action of MJF on tumor immunology in the treatment of HCC.METHODS The absorbable ingredients of MJF were identified using Molecule Network related to High Performance Liquid Chromatography-Electron Spray Ionization-Time of Flight-Mass Spectrometry,and hub potential anti-HCC targets were screened using network pharmacology and pathway enrichment analysis.Forty male mice were randomly divided into the Blank,Model,and MJF groups(1.8,5.4,and 10.8 g/kg/d)following 7 d of oral administration.Average body weight gain,spleen and thymus indices were calculated,tumor tissues were stained with hematoxylin and eosin,and Interferon gamma(IFN-γ),Tumor necrosis factorα(TNF-α),Interleukin-2,aspartate aminotransferase,alanine aminotransferase,alpha-fetoprotein(AFP),Fas,and FasL were measured by Enzyme-linked Immunosorbent Assay.Relevant mRNA expression of Bax and Bcl2 was evaluated by Real Time Quantitative PCR(RTqPCR)and protein expression of Transforming growth factorβ1(TGF-β1)and Mothers against decapentaplegic homolog(SMAD)4 was assessed by Western blotting.The HepG2 cell line was treated with 10 mg/mL,20 mg/mL,30 mg/mL,40 mg/mL of MJF,and another 3 groups were treated with TGF-β1 inhibitor(LY364947)and different doses of MJF.Relevant mRNA expression of TNF-α,IFN-γ,Bax and Bcl2 was evaluated by RT-qPCR and protein expression of TGF-β1,SMAD2,p-SMAD2,SMAD4,and SMAD7 was assessed by Western blotting.RESULTS It was shown that MJF improved body weight gain and tumor inhibition rate in H22 tumorbearing mice,protected immune organs and liver function,reduced the HCC indicator AFP,affected immunity and apoptosis,and up-regulated the TGF-β1/SMAD signaling pathway,by increasing the relative expression of TGF-β1,SMAD2,p-SMAD2 and SMAD4 and decreasing SMAD7,reducing immune factors TNF-αand IFN-γ,decreasing apoptosis cytokines Fas,FasL and Bcl2/Bax,and inhibiting the effect of LY364947 in HepG2 cells.CONCLUSION MJF inhibits HCC by activating the TGF-β1/SMAD signaling pathway,and affecting immune and apoptotic cytokines,which may be due to MJF adjusting immune escape and apoptosis.展开更多
[目的/意义]专利价值评估涉及到专利技术引进、开发、实施、许可、转让和融资等企业专利技术管理的各个环节。因此,专利价值研究成果是企业专利技术管理研究的重要组成部分。[方法/过程]基于Web of Science数据库,利用CiteSpace软件,从...[目的/意义]专利价值评估涉及到专利技术引进、开发、实施、许可、转让和融资等企业专利技术管理的各个环节。因此,专利价值研究成果是企业专利技术管理研究的重要组成部分。[方法/过程]基于Web of Science数据库,利用CiteSpace软件,从发文时间分布、主要发文国家、被引频次、关键文献、高频关键词和突变术语等角度,对国内外关于专利价值的研究成果进行分析,进而探求专利技术管理的发展历程、核心问题、演进路径、研究热点和前沿趋势。[结果/结论]专利价值相关研究成果和人员主要来源于美国、中国、英国和德国等国家,专利价值研究是企业专利技术管理的重要构成部分,法律价值是专利价值的前提和保障,而专利引证是企业专利技术管理及应用的前沿与趋势。展开更多
文摘This study was performed in order to determine visitation at remote areas that would be unaffordable due to logistic reasons. Four TrafxTM vehicle counters, each programmed with different settings, were placed along the lone access road to remotely sense the daily use activities and count accuracy at the New Underwood Lake Public Water Access Area. Use was corroborated during daylight hours with game cameras. Data was stratified between weekdays/weekends due to differences between the two periods. Two counter settings, threshold and delay, were best when set at a value of 8, but a value of 16 for delay provided almost equal results. Overall, there were 38 counts of use per day for a total of 2318 over the 61 day period. This study demonstrated how vehicle counters, in combination with game cameras for verification, can aid managers for determining use in remote access areas. Future work may lead to identifying details for producing a surrogate to traditional angler use surveys.
基金Liaoning Pharmaceutical Industry Innovation and Development Strategy Research and Funding Project(2020lslktyb-095)National Medical Products Administration-Special Fund of Drug Regulatory Research Institute of Shenyang Pharmaceutical University(2021jgkx004)+1 种基金Shenzhen Maternity&Child Healthcare Hospital Science Foundation(2022(73))Shenzhen Health Economics Society Research Fund Project(202333).
文摘Objective To discuss the problems existing in the compensation of Chinese clinical trial participants and propose some suggestions for improving their rights.Methods The literature related to the participants’right to compensation at home and abroad was searched to study the inadequacy of the compensation right for clinical trial participants in China from four aspects:insurance system,principle of attribution,legal relationship and compensation regulations.Then,some suggestions to improve the participants’right to compensation were proposed.Results and Conclusion China lacks clear legal norms for participants’right to compensation.There are problems such as unclear insurance rules and compensation rules,unclear contractual relationships between parties to clinical trials,and no laws and regulations to rely on for attribution and compensation.China should issue regulatory guidelines related to the right to compensation of participants in clinical trials,so that all parties in clinical trials can have rules to follow if there is the occurrence of injury,which can better protect the rights and interests of the participants.
基金Supported by National Natural Science Foundation of China,No.81874342Natural Science Foundation of Liaoning Province,No.2020-MZLH-35.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharides.MJF has been used in the clinical treatment of hepatitis,cirrhosis and HCC for more than 30 years.Few previous studies have focused on the mechanism of MJF on tumor immunology in the treatment of HCC.AIM To explore the mechanism of action of MJF on tumor immunology in the treatment of HCC.METHODS The absorbable ingredients of MJF were identified using Molecule Network related to High Performance Liquid Chromatography-Electron Spray Ionization-Time of Flight-Mass Spectrometry,and hub potential anti-HCC targets were screened using network pharmacology and pathway enrichment analysis.Forty male mice were randomly divided into the Blank,Model,and MJF groups(1.8,5.4,and 10.8 g/kg/d)following 7 d of oral administration.Average body weight gain,spleen and thymus indices were calculated,tumor tissues were stained with hematoxylin and eosin,and Interferon gamma(IFN-γ),Tumor necrosis factorα(TNF-α),Interleukin-2,aspartate aminotransferase,alanine aminotransferase,alpha-fetoprotein(AFP),Fas,and FasL were measured by Enzyme-linked Immunosorbent Assay.Relevant mRNA expression of Bax and Bcl2 was evaluated by Real Time Quantitative PCR(RTqPCR)and protein expression of Transforming growth factorβ1(TGF-β1)and Mothers against decapentaplegic homolog(SMAD)4 was assessed by Western blotting.The HepG2 cell line was treated with 10 mg/mL,20 mg/mL,30 mg/mL,40 mg/mL of MJF,and another 3 groups were treated with TGF-β1 inhibitor(LY364947)and different doses of MJF.Relevant mRNA expression of TNF-α,IFN-γ,Bax and Bcl2 was evaluated by RT-qPCR and protein expression of TGF-β1,SMAD2,p-SMAD2,SMAD4,and SMAD7 was assessed by Western blotting.RESULTS It was shown that MJF improved body weight gain and tumor inhibition rate in H22 tumorbearing mice,protected immune organs and liver function,reduced the HCC indicator AFP,affected immunity and apoptosis,and up-regulated the TGF-β1/SMAD signaling pathway,by increasing the relative expression of TGF-β1,SMAD2,p-SMAD2 and SMAD4 and decreasing SMAD7,reducing immune factors TNF-αand IFN-γ,decreasing apoptosis cytokines Fas,FasL and Bcl2/Bax,and inhibiting the effect of LY364947 in HepG2 cells.CONCLUSION MJF inhibits HCC by activating the TGF-β1/SMAD signaling pathway,and affecting immune and apoptotic cytokines,which may be due to MJF adjusting immune escape and apoptosis.
文摘[目的/意义]专利价值评估涉及到专利技术引进、开发、实施、许可、转让和融资等企业专利技术管理的各个环节。因此,专利价值研究成果是企业专利技术管理研究的重要组成部分。[方法/过程]基于Web of Science数据库,利用CiteSpace软件,从发文时间分布、主要发文国家、被引频次、关键文献、高频关键词和突变术语等角度,对国内外关于专利价值的研究成果进行分析,进而探求专利技术管理的发展历程、核心问题、演进路径、研究热点和前沿趋势。[结果/结论]专利价值相关研究成果和人员主要来源于美国、中国、英国和德国等国家,专利价值研究是企业专利技术管理的重要构成部分,法律价值是专利价值的前提和保障,而专利引证是企业专利技术管理及应用的前沿与趋势。
