Effects of Da Ding Feng Zhu Decoction in 30 Cases of Liver Fibrosis

To study the clinical effects of Da Ding Feng Zhu (大定风珠) Decoction on liver fibrosis. 56 patients withliver fibrosis due to chronic hepatitis B were randomly divided into a treatment group (30 cases treatedwith Da Ding Feng Zhu Decoction) and a control group (26 cases treated with colchicine). The serumlevels of hyaluronic acid (HA), procollagen Ⅲ (PC-Ⅲ), Ⅳ collagen (Ⅳ-C) and Laminin (LN) of thepatients were determined, compared and analyzed before treatment and after 3-month treatment in the twogroups. The results showed that in the treatment group, the levels of HA, PC-Ⅲ, Ⅳ-C and LN after3-month treatment were significantly lowered as compared to that before treatment (P＜0.01). In thecontrol group, only the HA level was obviously lowered (P＜0.05). There was an significant difference(P＜0.05) in PC-Ⅲ and Ⅳ-C and a very significant difference (P＜0.01) in HA after treatment between thetwo groups. It is therefore concluded that Da Ding Feng Zhu Decoction can lower serum indexes of liverfibrosis.

Evaluating the clinical efficacy of Thunder-Fire moxibustion combined with ovulation monitoring in the treatment of adenomyosis combined with infertility

目的:评价雷火灸联合排卵监测治疗子宫腺肌病合并不孕的临床疗效。方法:选择诊断符合子宫腺肌病合并不孕且中医辨证属于寒凝血瘀(具有经前综合症或经期下腹冷痛,得热痛减,肢端冰冷等临床表现)的患者120例,随机分为治疗组和对照组,每组各60例。治疗组予雷火灸联合排卵监测,对照组单纯予排卵监测,比较两组6个月经周期内的妊娠率,痛经和中医证候群的改善情况以及血清癌抗原125的水平。结果:治疗组6个月经周期内妊娠率与对照组相比有统计学意义(50.0%vs 23.3%,P=0.021)。与对照组相比,治疗组痛经与中医证候群改善明显(分别为3.87±2.03 vs.5.70±1.01,P=0.002 and 7.33±4.11 vs.10.52±2.33,P=0.006)。两组相比血清癌抗原125的水平有显著性差异(55.45±14.65 vs 63.34±11.41,P=0.031)。但是子宫平均直径与对照组相比差异无统计学意义(67.13±7.59 vs.69.89±5.30,P=0.137)。结论:雷火灸联合排卵监测能有效地提高子宫腺肌症不孕患者的受孕率。

Pathogenesis and treatment status of adenomyosis complicated with infertility

Adenomyosis (AD) refers to the endometrium (including glands and stroma) into myometrium gr owth of pathological changes, with the delay of women's childbea ring age, the pa ti ents with AD-associated infertility increased significantly. Although scholars disagreed about the effect of AD on infertility, more and more evidence showed that AD was closely related to the str uct ural and func ti onal defects of the eutopic endomet rium and myomet rial junc tional zone (MJZ), which may lead to embryo implantation failure and infertility. With the rapid development of imaging technology, the accuracy of diagnose AD has been enhanced, more and more infertility patients were diagnosed with AD. At the same time, a series of pathogenic hypothesis have been proposed to explain the relationship between AD. In addition, AD may influence the patient's reproductive function by affecting sperm transport, embryo implantation, altering uterine peristalsis, influencing the structure and function of endometrium, and changing the intrauterine environment. At present, the treatment of AD-associated infertility is similar to the treatment of AD. The commonly used treatment methods include drug therapy, conservative operative treatment, drug combination surgery, and Chinese medicine treatment and so on. The clinical and experimental studies of AD complicated with infertility and its possible pathogenesis and treatment in recent years are reviewed in this article.

Observation on TCM syndromes effect of Shujinjianyao Pill in the Treatment of Lumbar Spinal Stenosis (Ganshenbuzu and Fengshiyuzu Syndrome)

Objective:To observe the TCM(Traditonal Chinese Medcine)syndromes effect and safety of Shujinjianyao Pill in the treatment of LSS(Lumbar Spinal Stenosis)(Ganshenbuzu and Fengshiyuzu Syndrome).Methods:120 LSS patients fit the inclusion criteria were separated into two groups,90 in the experimental group and 30 in the control group.No significant difference was existed in the basic data between the two groups.The experimental group was given Shujinjianyao Pill and Danlutongdu Tablet Simulator orally,while the control group was given Shujinjianyao Pill Simulator and Danlutongdu Tablet orally for 4 weeks.The VAS score,total TCM(Traditonal Chinese Medcine)syndrome score and single TCM symptom score on day 0,14 and 28 were observed,and adverse reactions were recorded.Result:There were significant differences in total TCM syndrome score between the two groups at baseline,14 day and 28 day(P<0.01).There was a significant difference in total TCM syndrome score between the two groups at 28 day(P<0.05).Significant differences were existed between this two groups in total effective rate of total TCM syndromes and waist-knee pain-weak in single TCM syndromes on the 28 day(P<0.05).There was no significant difference in the incidence of adverse events and serious adverse events between the two groups(P>0.05).Conclusion:Shujinjianyao Pill is effective on TCM syndromes in the treatment of Ganshenbuzu and fengshiyuzu syndrome LSS,especially in improving the score of total TCM syndromes and waist-knee pain-weak of single TCM syndrome,and has high safety.

Effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients

Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.

Autophagy plays a pro-apoptotic role in arsenic trioxide-induced cell death of liver cancer

Objective: The effects of arsenic trioxide(As2O3) on hepatocellular carcinoma have been documented widely. Autophagy plays dual roles in the survival and death of cancer cells. Therefore, we investigated the exact role of autophagy in As2O3-induced apoptosis in liver cancer cells.Methods: The viability of hepatoma cells was determined using the MTT assay with or without fetal bovine serum. The rate of apoptosis in liver cancer cells treated with As2O3was evaluated using flow cytometry, Hoechst 33258 staining, and TUNEL assays. The rate of autophagy among liver cancer cells treated with As2O3was detected using immunofluorescence, Western blot assay and transmission electron microscopy.Results: Upon treatment with As2O3, the viability of HepG2 and SMMC-7721 cells was decreased in a time-and dose-dependent manner. The apoptosis rates of both liver cancer cell lines increased with the concentration of As2O3, as shown by flow cytometry. Apoptosis in liver cancer cells treated with As2O3was also shown by the activation of the caspase cascade and the regulation of Bcl-2/Bax expression.Furthermore, As2O3treatment induced autophagy in liver cancer cells;this finding was supported by Western blot, immunofluorescence of LC3-II and beclin 1, and transmission electron microscopy. In liver cancer cells, As2O3inhibited the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR) signal pathway that plays a vital role in both apoptosis and autophagy. The PI3K activator SC-79 partially reversed As2O3-induced autophagy and apoptosis. Furthermore, inhibiting autophagy with 3-methyladenine partially reversed the negative effects of As2O3on cell viability.Serum starvation increased autophagy and amplified the effect of As2O3on cell death.Conclusion: As2O3induces apoptosis and autophagy in liver cancer cells. Autophagy induced by As2O3may have a proapoptotic effect that helps to reduce the viability of liver cancer cells. This study provides novel insights into the effects of As2O3against liver cancer.

Efficacy and safety of LY01005 versus goserelin implant in Chinese patients with prostate cancer: A multicenter, randomized, open-label, phase III, non-inferiority trial

Background:LY01005(Goserelin acetate sustained-release microsphere injection)is a modified gonadotropin-releasing hormone(GnRH)agonist injected monthly.This phase III trial study aimed to evaluated the efficacy and safety of LY01005 in Chinese patients with prostate cancer.Methods:We conducted a randomized controlled,open-label,non-inferiority trial across 49 sites in China.This study included 290 patients with prostate cancer who received either LY01005 or goserelin implants every 28 days for three injections.The primary efficacy endpoints were the percentage of patients with testosterone suppression≤50 ng/dL at day 29 and the cumulative probability of testosterone≤50 ng/dL from day 29 to 85.Non-inferiority was prespecified at a margin of-10%.Secondary endpoints included significant castration(≤20 ng/dL),testosterone surge within 72 h following repeated dosing,and changes in luteinizing hormone,follicle-stimulating hormone,and prostate specific antigen levels.Results:On day 29,in the LY01005 and goserelin implant groups,testosterone concentrations fell below medical-castration levels in 99.3%(142/143)and 100%(140/140)of patients,respectively,with a difference of-0.7%(95%confidence interval[CI],-3.9%to 2.0%)between the two groups.The cumulative probabilities of maintaining castration from days 29 to 85 were 99.3%and 97.8%,respectively,with a between-group difference of 1.5%(95%CI,-1.3%to 4.4%).Both results met the criterion for non-inferiority.Secondary endpoints were similar between groups.Both treatments were well-tolerated.LY01005 was associated with fewer injection-site reactions than the goserelin implant(0%vs.1.4%[2/145]).Conclusion:LY01005 is as effective as goserelin implants in reducing testosterone to castration levels,with a similar safety profile.Trial registration:ClinicalTrials.gov,NCT04563936.

Thinking on Functional Mechanism of Acupuncture for Inflammatory Bowel Diseases Based on Metabolomics

Acupuncture has a good therapeutic effect in treatment of inflammatory bowel diseases(IBD), but its functional mechanism has not been systematically explained. Metabolomics is the scientific study of dynamic chemical processes involving metabolites as well as metabolic response of living organisms. Metabolomics, a research method with integrity and dynamics, corresponds to the overall regulatory effect of acupuncture and is in line with the overall concept of traditional Chinese medicine(TCM) and the concept of homeostasis. In the recent years, metabolomics has been extensively applied to the clinical and experimental study of IBD, and its potential applied value has been unanimously acknowledged by the researchers. In this article, the application status of metabolomics in acupuncture is summarized, and the research ideas to study the mechanism of acupuncture in the regulation and control of IBD by metabolomics are preliminarily explored.

Observations on the Efficacy of Mind-Calming and Brain-Refreshing Acupuncture and Moxibustion for Treating 42 Anxiety Patients

目的:观察针刺配合艾灸治疗焦虑症的疗效。方法:以针刺配合艾灸治疗焦虑症42例为治疗组,同时随机设西药治疗45例为对照组,进行对照观察。结果:治疗组总有效率92.9%,对照组71.1%,两组差异有显著性意义(P<0.01)。结论:治疗组明显优于对照组,且无副作用和药物依赖性。

Hypoxia-induced ROS aggravate tumor progression through HIF-1α-SERPINE1 signaling in glioblastoma

Hypoxia,as an important hallmark of the tumor microenvironment,is a major cause of oxidative stress and plays a central role in various malignant tumors,including glioblastoma.Elevated reactive oxygen species(ROS)in a hypoxic microenvironment promote glioblastoma progression;however,the underlying mechanism has not been clarified.Herein,we found that hypoxia promoted ROS production,and the proliferation,migration,and invasion of glioblastoma cells,while this promotion was restrained by ROS scavengers N-acetyl-L-cysteine(NAC)and diphenyleneiodonium chloride(DPI).Hypoxia-induced ROS activated hypoxia-inducible factor-1α(HIF-1α)signaling,which enhanced cell migration and invasion by epithelial-mesenchymal transition(EMT).Furthermore,the induction of serine protease inhibitor family E member 1(SERPINE1)was ROS-dependent under hypoxia,and HIF-1αmediated SERPINE1 increase induced by ROS via binding to the SERPINE1 promoter region,thereby facilitating glioblastoma migration and invasion.Taken together,our data revealed that hypoxia-induced ROS reinforce the hypoxic adaptation of glioblastoma by driving the HIF-1α-SERPINE1 signaling pathway,and that targeting ROS may be a promising therapeutic strategy for glioblastoma.

推拿结合远道取穴针刺治疗青少年假性近视(英文)

目的:观察推拿结合远道取穴针刺治疗青少年假性近视的临床疗效。方法:将64名青少年假性近视患者按随机数字表随机分为治疗组和对照组,每组32例(64只眼)。治疗组采用推拿结合远道取穴针刺治疗,对照组采用单纯推拿治疗,推拿手法、推拿部位及治疗时间均与治疗组相同。每日治疗1次,连续10次为1疗程,休息2 d后继续下一个疗程,共治疗3个疗程。两组患者均在接受3疗程治疗后进行标准对数视力表检测裸眼远视力和散瞳后检影验光近视屈光度检测,观察其近期疗效。结果:治疗3个疗程后,两组患者视力均较治疗前有改善;治疗组总有效率为92.2%,对照组为82.8%,两组总有效率差异有统计学意义(P<0.05),提示治疗组疗效优于对照组。结论:推拿结合远道取穴针刺治疗青少年假性近视疗效显著,而且没有针刺眼周穴位带来的恐惧,易于被青少年接受,值得在临床上推广应用。

电针夹脊穴配合中药治疗带状疱疹疗效观察(英文)

目的:观察电针夹脊穴配合中药治疗带状疱疹的临床疗效。方法:将62例患者随机分为治疗组和对照组,治疗组予电针夹脊穴配合中药辨证治疗,对照组采用口服阿昔洛韦片抗病毒和甲钴胺营养神经、皮疹外涂阿昔洛韦软膏治疗;治疗2星期后比较两组疗效。结果:治疗组治疗1星期后疗效显著优于对照组(P<0.01),治疗2星期后两组疗效亦有差异(P<0.05)。结论:电针夹脊穴配合中药治疗带状疱疹疗效优于西医抗病毒、营养神经的治疗。

针刺配合铜管灸治疗复发性周围性面瘫39例(英文)

目的:观察针刺配合铜管灸治疗复发性周围性面瘫临床疗效。方法:共纳入39例复发性周围性面瘫患者,给予针刺配合自制铜管灸器灸,每日治疗1次,10天为1疗程,疗程间休息3d,治疗4个疗程后统计疗效。结果:39例中痊愈18例,好转15例,未愈6例,总有效率84.6%。结论:针刺配合铜管灸治疗复发性周围性面瘫疗效肯定。

颈椎微调推拿手法配合舒颈定眩汤治疗颈性眩晕（英文）

目的:观察颈椎微调推拿手法配合舒颈定眩汤治疗颈性眩晕的疗效。方法:采用随机、开放、平行对照研究。治疗组予以颈椎微调推拿手法配合舒颈定眩汤治疗,对照组单以推拿治疗。两个疗程后进行统计。结果:治疗组和对照组治愈率分别为20.6%和14.7%;总有效率分别为88.2%和73.5%。两组治愈率及总有效率差异均有统计学意义(P<0.01)。结论:颈椎微调推拿手法配合舒颈定眩汤治疗颈性眩晕疗效优于单纯颈椎微调推拿手法治疗。