Comparative optical study of biofluids(serum,urine,hemodialyste)by concentration change ofendogenous visible fluorescence substance(VFS)has been carried out.Biofluids were collectedfrom chronic kidney diseases(CKD)pat...Comparative optical study of biofluids(serum,urine,hemodialyste)by concentration change ofendogenous visible fluorescence substance(VFS)has been carried out.Biofluids were collectedfrom chronic kidney diseases(CKD)patients(Pts)_as well as from healthy controls(HCs).Exeitation/emission spectra are similar for all samples of biofluids differing only in intensity,which is higher for CKD Pts.Strong similarity enables the study of given biofluids from a unitedphysical platform,proposed earlier,i.e.,as nanoparticles approach.Specific spectral redistribu-tion of visible fluorescence(VF)intensity as a result of dilution is revealed.The concentrationchange of VFS by dilution of samples manifests in nonlinear dependences in the scales"VFintensity-concentration"for serum and urine and in perfect linearity for hemodialysate(HD).The latter fact can be used in monitoring of hemodialysis procedure.展开更多
Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth ...Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.展开更多
基金support for this work by the Archimedes project 3.2.1101.12-0011.
文摘Comparative optical study of biofluids(serum,urine,hemodialyste)by concentration change ofendogenous visible fluorescence substance(VFS)has been carried out.Biofluids were collectedfrom chronic kidney diseases(CKD)patients(Pts)_as well as from healthy controls(HCs).Exeitation/emission spectra are similar for all samples of biofluids differing only in intensity,which is higher for CKD Pts.Strong similarity enables the study of given biofluids from a unitedphysical platform,proposed earlier,i.e.,as nanoparticles approach.Specific spectral redistribu-tion of visible fluorescence(VF)intensity as a result of dilution is revealed.The concentrationchange of VFS by dilution of samples manifests in nonlinear dependences in the scales"VFintensity-concentration"for serum and urine and in perfect linearity for hemodialysate(HD).The latter fact can be used in monitoring of hemodialysis procedure.
基金support for this work by the Archimedes project 3.2.1101.12-0011supported in part by the institutional research funding IUT (IUT 2-26)of the Estonian Ministry of Education and Research.
文摘Solid phases of visible fuorescence substance(VFS)of biological fuids(blood,urine,hemodia-lysate)which was proposed earlier as a morbidity and mortality marker by renal failure and diabetes were investigated in-depth by the methods of electron and confocal microscopy,optical spectroscopy and matrix assisted laser desorption-ionization(MALDI)mass spectroscopy.It is shown that dry VFS exists predominantly in the form of carbon-oxygen-nitrogen(N≈8.7 wt.%)nanoparticles(NPs)(5≤d≤100nm).For the first time the existence of the threshold energy E_(g)≈2.15eV for excitation of VFS was observed experimentally and confirmed by semi empirical calculations of the bathochromic shift.A good accordance with the earlier autonomous theo-retical calculations was achieved.Thus,the long wavelength limit(575 nm)of the spectral range where the VFS can be used as a fluorescent marker was reliably determined.A pilot MALDI comparative study of graphene oxide(GO)and urine VFS was carried out.Six kinds of nitrogen-free particles(412≤M≤456 Da)were observed in each substance and possible computer models of those have been composed.It is established that along with nitrogen-containing advanced glycation end products(AGEs)also nitrogen-fee carbon-oxygen-lhydrogen particles(probably toxic)with the composition and structure related to GO can exist in biofuids.Both types of particles should be taken into account in search for the reasons of high mortality among end stage renal disease patients.
