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A reporter for noninvasively monitoring gene expression and plant transformation 被引量:14
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作者 Yubing He Tao Zhang +2 位作者 Hui Sun Huadong Zhan Yunde Zhao 《Horticulture Research》 SCIE 2020年第1期821-826,共6页
Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive treatments.Here... Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive treatments.Here,we construct a new reporter RUBY that converts tyrosine to vividly red betalain,which is clearly visible to naked eyes without the need of using special equipment or chemical treatments.We show that RUBY can be used to noninvasively monitor gene expression in plants.Furthermore,we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis.The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions. 展开更多
关键词 TRANSFORMATION REPORTER STERILE
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